Mutagenetix > Incidental Mutation

Incidental Mutation 'R8015:Vmn2r23'

617124

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r23

Ensembl Gene

ENSMUSG00000091620

Gene Name

vomeronasal 2, receptor 23

Synonyms

EG435916

MMRRC Submission

067455-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R8015 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

123702821-123742291 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 123704541 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Asparagine at position 136 (S136N)

Ref Sequence

ENSEMBL: ENSMUSP00000126682 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000172391]

AlphaFold

E9PXI5

Predicted Effect

probably benign
Transcript: ENSMUST00000172391
AA Change: S136N

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000126682
Gene: ENSMUSG00000091620
AA Change: S136N

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	79	461	1.7e-31	PFAM
Pfam:NCD3G	513	566	1.2e-23	PFAM
Pfam:7tm_3	596	834	1.5e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

100% (51/51)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Alad	A	G	4: 62,511,922 (GRCm38)	V121A	probably damaging	Het
Anapc2	T	A	2: 25,284,676 (GRCm38)	Y684N	probably benign	Het
Arntl2	T	C	6: 146,820,590 (GRCm38)	S220P	probably damaging	Het
Atp10a	G	T	7: 58,803,497 (GRCm38)	R808L	probably benign	Het
Best2	A	G	8: 85,009,354 (GRCm38)	V317A	probably damaging	Het
Cacnb4	A	G	2: 52,464,643 (GRCm38)	L241P	probably damaging	Het
Col6a5	T	C	9: 105,881,741 (GRCm38)	T2089A	possibly damaging	Het
Cttnbp2	G	C	6: 18,426,093 (GRCm38)	A762G	possibly damaging	Het
Defb13	G	T	8: 21,946,812 (GRCm38)	V8F	possibly damaging	Het
Dpp6	C	T	5: 26,817,810 (GRCm38)		probably benign	Het
Fam126b	A	G	1: 58,535,482 (GRCm38)	V300A	possibly damaging	Het
Fat4	A	G	3: 38,981,916 (GRCm38)	D3239G	possibly damaging	Het
Gm16486	C	T	8: 70,716,792 (GRCm38)	S1177L	probably benign	Het
Gm5615	A	G	9: 36,534,954 (GRCm38)	V54A	probably benign	Het
Hmcn1	T	G	1: 150,598,311 (GRCm38)	R4793S	possibly damaging	Het
Hspa1a	T	A	17: 34,970,649 (GRCm38)	Q426L	probably damaging	Het
Il20rb	T	A	9: 100,474,894 (GRCm38)	Y61F	probably damaging	Het
Itgb1	T	A	8: 128,722,401 (GRCm38)	V496D	possibly damaging	Het
Jakmip1	T	C	5: 37,159,765 (GRCm38)	S102P	unknown	Het
Jmjd8	T	A	17: 25,829,328 (GRCm38)	F108I	probably damaging	Het
Krtap15	A	G	16: 88,829,209 (GRCm38)	T55A	possibly damaging	Het
Lce3d	A	T	3: 92,958,503 (GRCm38)		probably benign	Het
Lnpep	C	A	17: 17,546,499 (GRCm38)	V702F	probably damaging	Het
Lox	G	A	18: 52,528,348 (GRCm38)	A218V	probably benign	Het
Mcrs1	T	A	15: 99,248,854 (GRCm38)	K99*	probably null	Het
Mfsd8	G	A	3: 40,846,835 (GRCm38)		probably benign	Het
Msto1	G	T	3: 88,911,556 (GRCm38)	P264Q	probably damaging	Het
Nrp2	C	T	1: 62,745,408 (GRCm38)	R239C	probably damaging	Het
Olfr1002	G	T	2: 85,647,792 (GRCm38)	H176Q	probably damaging	Het
Olfr1368	T	C	13: 21,142,133 (GRCm38)	D308G	probably benign	Het
Pate2	A	T	9: 35,670,518 (GRCm38)	H36L	probably damaging	Het
Phf20l1	T	G	15: 66,639,948 (GRCm38)	N925K	possibly damaging	Het
Pnliprp2	G	T	19: 58,766,282 (GRCm38)	V253F	probably damaging	Het
Polr2b	A	G	5: 77,336,506 (GRCm38)	D745G	probably damaging	Het
Ptdss1	T	A	13: 66,963,343 (GRCm38)	W158R	possibly damaging	Het
Rcn3	T	A	7: 45,084,907 (GRCm38)	I226F	probably damaging	Het
Rhbdl1	C	G	17: 25,835,851 (GRCm38)	V157L	probably damaging	Het
Slc30a2	A	G	4: 134,347,450 (GRCm38)	T173A	probably benign	Het
Spata16	A	G	3: 26,667,659 (GRCm38)	T110A	probably benign	Het
Specc1l	A	G	10: 75,241,068 (GRCm38)	K53E	probably benign	Het
Speg	G	T	1: 75,415,421 (GRCm38)		probably benign	Het
Spta1	T	A	1: 174,240,171 (GRCm38)	C2055S	probably damaging	Het
Tex14	G	A	11: 87,509,600 (GRCm38)	R406Q	probably benign	Het
Tmem150b	C	T	7: 4,716,328 (GRCm38)	G198S	probably null	Het
Tnfrsf14	T	G	4: 154,926,661 (GRCm38)	Q81P	probably damaging	Het
Txndc17	A	G	11: 72,207,742 (GRCm38)	K40R	probably benign	Het
Ube3a	T	A	7: 59,284,756 (GRCm38)	V560E	probably damaging	Het
Vmn1r14	A	G	6: 57,234,030 (GRCm38)	M198V	probably damaging	Het
Vmn2r31	C	T	7: 7,384,200 (GRCm38)	V791I	probably damaging	Het
Wdfy4	T	C	14: 33,107,747 (GRCm38)	Y984C		Het
Zfp386	T	G	12: 116,059,407 (GRCm38)	D248E	probably damaging	Het

Other mutations in Vmn2r23

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00324:Vmn2r23	APN	6	123,729,725 (GRCm38)	missense	possibly damaging	0.89
IGL01012:Vmn2r23	APN	6	123,729,596 (GRCm38)	missense	probably benign
IGL01073:Vmn2r23	APN	6	123,712,800 (GRCm38)	missense	possibly damaging	0.82
IGL01547:Vmn2r23	APN	6	123,704,424 (GRCm38)	missense	possibly damaging	0.88
IGL01571:Vmn2r23	APN	6	123,704,407 (GRCm38)	missense	probably damaging	1.00
IGL01950:Vmn2r23	APN	6	123,741,886 (GRCm38)	missense	possibly damaging	0.80
IGL02028:Vmn2r23	APN	6	123,741,860 (GRCm38)	missense	probably damaging	1.00
IGL02248:Vmn2r23	APN	6	123,741,744 (GRCm38)	missense	probably damaging	0.96
IGL02318:Vmn2r23	APN	6	123,741,836 (GRCm38)	missense	probably benign	0.10
IGL02649:Vmn2r23	APN	6	123,704,478 (GRCm38)	missense	probably benign
IGL02831:Vmn2r23	APN	6	123,704,385 (GRCm38)	missense	probably benign	0.22
IGL02832:Vmn2r23	APN	6	123,704,396 (GRCm38)	missense	probably benign	0.00
IGL02865:Vmn2r23	APN	6	123,741,619 (GRCm38)	missense	probably damaging	1.00
IGL02964:Vmn2r23	APN	6	123,741,782 (GRCm38)	missense	possibly damaging	0.93
IGL03347:Vmn2r23	APN	6	123,704,374 (GRCm38)	missense	probably benign	0.01
IGL03396:Vmn2r23	APN	6	123,729,626 (GRCm38)	missense	probably damaging	1.00
PIT4472001:Vmn2r23	UTSW	6	123,712,977 (GRCm38)	missense	possibly damaging	0.62
R0597:Vmn2r23	UTSW	6	123,729,721 (GRCm38)	missense	probably benign	0.08
R0677:Vmn2r23	UTSW	6	123,713,451 (GRCm38)	missense	probably benign	0.00
R0904:Vmn2r23	UTSW	6	123,742,135 (GRCm38)	missense	probably damaging	1.00
R1330:Vmn2r23	UTSW	6	123,742,004 (GRCm38)	missense	probably damaging	1.00
R1424:Vmn2r23	UTSW	6	123,713,270 (GRCm38)	nonsense	probably null
R1629:Vmn2r23	UTSW	6	123,713,427 (GRCm38)	missense	probably benign	0.05
R1842:Vmn2r23	UTSW	6	123,729,690 (GRCm38)	missense	possibly damaging	0.77
R1867:Vmn2r23	UTSW	6	123,702,915 (GRCm38)	missense	probably damaging	1.00
R1919:Vmn2r23	UTSW	6	123,713,010 (GRCm38)	missense	possibly damaging	0.94
R2087:Vmn2r23	UTSW	6	123,741,499 (GRCm38)	missense	probably benign	0.00
R2338:Vmn2r23	UTSW	6	123,704,425 (GRCm38)	missense	possibly damaging	0.88
R2568:Vmn2r23	UTSW	6	123,742,188 (GRCm38)	nonsense	probably null
R2867:Vmn2r23	UTSW	6	123,713,164 (GRCm38)	missense	possibly damaging	0.94
R2867:Vmn2r23	UTSW	6	123,713,164 (GRCm38)	missense	possibly damaging	0.94
R3500:Vmn2r23	UTSW	6	123,713,170 (GRCm38)	missense	possibly damaging	0.81
R3789:Vmn2r23	UTSW	6	123,741,389 (GRCm38)	missense	probably damaging	1.00
R4164:Vmn2r23	UTSW	6	123,729,738 (GRCm38)	missense	probably benign
R4506:Vmn2r23	UTSW	6	123,702,925 (GRCm38)	missense	probably damaging	1.00
R4652:Vmn2r23	UTSW	6	123,741,730 (GRCm38)	missense	probably damaging	1.00
R4697:Vmn2r23	UTSW	6	123,741,826 (GRCm38)	missense	probably damaging	1.00
R4840:Vmn2r23	UTSW	6	123,713,074 (GRCm38)	missense	probably damaging	1.00
R4983:Vmn2r23	UTSW	6	123,733,349 (GRCm38)	missense	probably damaging	1.00
R5276:Vmn2r23	UTSW	6	123,712,977 (GRCm38)	missense	possibly damaging	0.62
R5392:Vmn2r23	UTSW	6	123,704,364 (GRCm38)	missense	probably benign	0.36
R5528:Vmn2r23	UTSW	6	123,713,002 (GRCm38)	missense	probably damaging	1.00
R5529:Vmn2r23	UTSW	6	123,713,451 (GRCm38)	missense	probably benign	0.00
R5664:Vmn2r23	UTSW	6	123,713,074 (GRCm38)	missense	probably damaging	1.00
R5749:Vmn2r23	UTSW	6	123,733,273 (GRCm38)	missense	probably benign
R5761:Vmn2r23	UTSW	6	123,712,759 (GRCm38)	missense	probably benign	0.39
R5762:Vmn2r23	UTSW	6	123,733,393 (GRCm38)	missense	probably damaging	1.00
R5868:Vmn2r23	UTSW	6	123,712,942 (GRCm38)	missense	probably benign	0.12
R5935:Vmn2r23	UTSW	6	123,741,895 (GRCm38)	missense	possibly damaging	0.94
R6242:Vmn2r23	UTSW	6	123,704,400 (GRCm38)	missense	possibly damaging	0.82
R6416:Vmn2r23	UTSW	6	123,712,902 (GRCm38)	missense	probably damaging	1.00
R6524:Vmn2r23	UTSW	6	123,713,425 (GRCm38)	missense	probably damaging	1.00
R6576:Vmn2r23	UTSW	6	123,733,273 (GRCm38)	missense	probably benign
R6925:Vmn2r23	UTSW	6	123,704,553 (GRCm38)	missense	probably damaging	1.00
R7148:Vmn2r23	UTSW	6	123,713,022 (GRCm38)	missense	probably benign
R7215:Vmn2r23	UTSW	6	123,704,364 (GRCm38)	missense	probably benign	0.36
R7252:Vmn2r23	UTSW	6	123,741,581 (GRCm38)	missense	probably damaging	0.97
R7403:Vmn2r23	UTSW	6	123,704,579 (GRCm38)	missense	probably benign	0.01
R8143:Vmn2r23	UTSW	6	123,741,353 (GRCm38)	missense	probably damaging	0.99
R8474:Vmn2r23	UTSW	6	123,704,640 (GRCm38)	missense	probably benign	0.36
R8520:Vmn2r23	UTSW	6	123,741,656 (GRCm38)	missense	probably damaging	0.99
R8679:Vmn2r23	UTSW	6	123,713,472 (GRCm38)	missense	probably damaging	0.99
R8713:Vmn2r23	UTSW	6	123,703,032 (GRCm38)	missense
R8966:Vmn2r23	UTSW	6	123,742,120 (GRCm38)	missense	possibly damaging	0.94
R9124:Vmn2r23	UTSW	6	123,742,079 (GRCm38)	missense	possibly damaging	0.57
R9163:Vmn2r23	UTSW	6	123,741,823 (GRCm38)	missense	probably damaging	1.00
R9189:Vmn2r23	UTSW	6	123,704,364 (GRCm38)	missense	probably benign	0.36
R9451:Vmn2r23	UTSW	6	123,733,393 (GRCm38)	missense	probably damaging	1.00
R9495:Vmn2r23	UTSW	6	123,712,713 (GRCm38)	missense	probably benign	0.30
R9514:Vmn2r23	UTSW	6	123,712,713 (GRCm38)	missense	probably benign	0.30
RF018:Vmn2r23	UTSW	6	123,713,116 (GRCm38)	missense	probably benign	0.00
T0975:Vmn2r23	UTSW	6	123,713,161 (GRCm38)	missense	probably benign	0.00
Z1088:Vmn2r23	UTSW	6	123,742,108 (GRCm38)	missense	probably damaging	0.98
Z1177:Vmn2r23	UTSW	6	123,729,725 (GRCm38)	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- CAGCACATGTTGGCAATGG -3'
(R):5'- GCAAAGGACATTTTCTTGGGAGTG -3'

Sequencing Primer
(F):5'- CAGCACATGTTGGCAATGGTTTTTG -3'
(R):5'- CATTTTCTTGGGAGTGGTAAGAATAC -3'

Posted On

2020-01-23