Mutagenetix > Incidental Mutation

Incidental Mutation 'R8022:Oas3'

617443

Institutional Source

Beutler Lab

Gene Symbol

Oas3

Ensembl Gene

ENSMUSG00000032661

Gene Name

2'-5' oligoadenylate synthetase 3

Synonyms

Oasl10, 2'-5' oligoadenylate synthetase-like 10

MMRRC Submission

067461-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.092) question?

Stock #

R8022 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

120891163-120915726 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 120895031 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 986 (I986T)

Ref Sequence

ENSEMBL: ENSMUSP00000035588 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044833] [ENSMUST00000165820]

AlphaFold

Q8VI93

Predicted Effect

possibly damaging
Transcript: ENSMUST00000044833
AA Change: I986T

PolyPhen 2 Score 0.908 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000035588
Gene: ENSMUSG00000032661
AA Change: I986T

Domain	Start	End	E-Value	Type
Pfam:OAS1_C	159	341	6.3e-83	PFAM
Pfam:OAS1_C	610	795	3.1e-78	PFAM
Pfam:NTP_transf_2	831	920	4.5e-11	PFAM
Pfam:OAS1_C	954	1136	9e-85	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000165820

SMART Domains

Protein: ENSMUSP00000125873
Gene: ENSMUSG00000032661

Domain	Start	End	E-Value	Type
Pfam:OAS1_C	1	130	3.8e-53	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an enzyme included in the 2', 5' oligoadenylate synthase family. This enzyme is induced by interferons and catalyzes the 2', 5' oligomers of adenosine in order to bind and activate RNase L. This enzyme family plays a significant role in the inhibition of cellular protein synthesis and viral infection resistance. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 87 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acacb	A	T	5: 114,361,915 (GRCm39)	T1386S	probably benign	Het
AI661453	T	G	17: 47,777,161 (GRCm39)	S296A	unknown	Het
Ambp	G	T	4: 63,062,434 (GRCm39)	N268K	probably damaging	Het
Ankrd11	T	C	8: 123,614,332 (GRCm39)	K2503E	probably damaging	Het
Ap1g1	C	A	8: 110,559,367 (GRCm39)	R221S	possibly damaging	Het
Ap5z1	T	C	5: 142,455,904 (GRCm39)		probably null	Het
Aspa	A	C	11: 73,213,032 (GRCm39)	N103K	probably benign	Het
Bend7	G	A	2: 4,757,590 (GRCm39)	V211I	probably benign	Het
Bsn	A	G	9: 107,991,603 (GRCm39)	M1383T	probably benign	Het
Cad	G	T	5: 31,226,150 (GRCm39)	V1117F	probably damaging	Het
Cdh2	A	G	18: 16,723,358 (GRCm39)	L856S	probably damaging	Het
Cdh20	T	A	1: 109,988,838 (GRCm39)	S247T	probably benign	Het
Ces1b	A	G	8: 93,795,943 (GRCm39)		probably null	Het
Chd7	T	A	4: 8,751,605 (GRCm39)	V34E	unknown	Het
Clca3a2	A	T	3: 144,511,527 (GRCm39)	F623I	probably damaging	Het
Cope	T	G	8: 70,765,453 (GRCm39)	M217R	probably benign	Het
Crim1	T	A	17: 78,622,984 (GRCm39)	I394N	possibly damaging	Het
Crnkl1	A	T	2: 145,760,486 (GRCm39)	I644N	probably damaging	Het
Cry1	G	A	10: 84,982,266 (GRCm39)	A360V	probably damaging	Het
Ctcfl	A	T	2: 172,960,559 (GRCm39)	V8D	probably benign	Het
Cyp2d34	G	A	15: 82,500,315 (GRCm39)	Q475*	probably null	Het
Cyp39a1	T	C	17: 44,057,468 (GRCm39)	Y436H	probably damaging	Het
Cyp3a25	T	A	5: 145,914,478 (GRCm39)	Q484L	probably benign	Het
Dnah1	A	G	14: 30,986,971 (GRCm39)	F3607S	probably damaging	Het
Dnali1	T	A	4: 124,959,323 (GRCm39)	K23N	possibly damaging	Het
Ecel1	T	C	1: 87,081,052 (GRCm39)	I313V	probably benign	Het
Ehhadh	C	A	16: 21,596,570 (GRCm39)	A53S	probably benign	Het
Epcam	T	C	17: 87,953,736 (GRCm39)	S277P	probably benign	Het
Fbxo10	T	C	4: 45,062,062 (GRCm39)	I155V	possibly damaging	Het
Fgd6	A	T	10: 93,880,206 (GRCm39)	K353N	possibly damaging	Het
Glce	A	T	9: 61,967,873 (GRCm39)	M426K	probably benign	Het
Glmp	A	T	3: 88,233,827 (GRCm39)	N228I	probably damaging	Het
Gm4787	C	A	12: 81,424,494 (GRCm39)	V555F	possibly damaging	Het
Gm5114	T	A	7: 39,058,800 (GRCm39)	H273L	probably benign	Het
Gzmg	T	A	14: 56,394,903 (GRCm39)	T122S	probably benign	Het
Hace1	T	C	10: 45,577,066 (GRCm39)	V820A	probably damaging	Het
Igf2r	T	A	17: 12,937,682 (GRCm39)	D535V	probably damaging	Het
Kcnd3	A	G	3: 105,366,189 (GRCm39)	M20V	probably benign	Het
Kcnn3	T	G	3: 89,517,010 (GRCm39)	I473S	possibly damaging	Het
Klhl35	T	A	7: 99,122,446 (GRCm39)	F94Y	unknown	Het
Kmt2c	C	T	5: 25,486,678 (GRCm39)	V4712I	possibly damaging	Het
Lepr	A	G	4: 101,639,754 (GRCm39)	E740G	probably benign	Het
Lmod3	T	C	6: 97,225,260 (GRCm39)	D187G	probably benign	Het
Lsm3	C	T	6: 91,496,543 (GRCm39)	H49Y	probably benign	Het
Lypd10	T	A	7: 24,413,599 (GRCm39)	I205N	possibly damaging	Het
Magi1	T	C	6: 93,674,346 (GRCm39)	S962G	probably damaging	Het
Man2b1	G	A	8: 85,822,242 (GRCm39)	R782Q	probably damaging	Het
Mical2	A	T	7: 111,902,974 (GRCm39)	K148N	probably damaging	Het
Nbeal1	C	T	1: 60,299,431 (GRCm39)	Q1256*	probably null	Het
Ncam1	G	T	9: 49,476,192 (GRCm39)	A299D	possibly damaging	Het
Ncapg	A	G	5: 45,839,136 (GRCm39)	D512G	probably damaging	Het
Nkiras2	A	G	11: 100,515,113 (GRCm39)	N28D	probably benign	Het
Nprl2	A	C	9: 107,420,260 (GRCm39)	K53T	probably damaging	Het
Nr4a3	T	A	4: 48,051,510 (GRCm39)	I88N	probably damaging	Het
Or6c76	A	T	10: 129,612,654 (GRCm39)	L305F	possibly damaging	Het
Pcsk1	A	T	13: 75,247,412 (GRCm39)	Y187F	possibly damaging	Het
Pgc	A	G	17: 48,039,701 (GRCm39)	T32A	probably benign	Het
Ranbp2	A	C	10: 58,321,683 (GRCm39)	D2660A	possibly damaging	Het
Retreg1	A	G	15: 25,843,565 (GRCm39)	R46G		Het
Rrbp1	C	A	2: 143,798,712 (GRCm39)	K1100N	probably benign	Het
Rsph10b	A	T	5: 143,904,050 (GRCm39)	T676S	probably benign	Het
Setdb1	A	T	3: 95,245,910 (GRCm39)	F672I	probably damaging	Het
Setdb1	T	A	3: 95,254,396 (GRCm39)	D195V	probably damaging	Het
Slc12a8	A	G	16: 33,445,456 (GRCm39)	E450G	probably benign	Het
Slc1a7	G	A	4: 107,869,473 (GRCm39)	V513M	probably benign	Het
Slc25a12	A	T	2: 71,105,533 (GRCm39)	V667E	unknown	Het
Slc39a3	T	C	10: 80,867,111 (GRCm39)	T212A	probably benign	Het
Slc45a1	C	T	4: 150,722,766 (GRCm39)	G373S	possibly damaging	Het
Snx11	G	A	11: 96,663,680 (GRCm39)	T53M	probably damaging	Het
Snx33	A	G	9: 56,832,624 (GRCm39)	F482L	possibly damaging	Het
Srebf2	C	T	15: 82,062,966 (GRCm39)	R468C	probably damaging	Het
Stk32a	C	T	18: 43,448,166 (GRCm39)	Q382*	probably null	Het
Sun3	C	A	11: 8,973,376 (GRCm39)	S167I	probably damaging	Het
Sycp2	A	G	2: 177,996,855 (GRCm39)	L1116P	probably damaging	Het
Thbs4	T	G	13: 92,888,955 (GRCm39)	T913P	probably damaging	Het
Trav8d-1	C	T	14: 53,016,284 (GRCm39)	Q57*	probably null	Het
Trio	C	G	15: 27,749,952 (GRCm39)	V2250L	probably benign	Het
Unc119b	A	T	5: 115,265,102 (GRCm39)	I204N	probably damaging	Het
Usp4	A	G	9: 108,255,670 (GRCm39)	E576G	probably damaging	Het
Uvssa	T	A	5: 33,566,848 (GRCm39)	L515Q	probably damaging	Het
Xkr8	T	C	4: 132,459,649 (GRCm39)	Y43C	probably damaging	Het
Xpo6	G	A	7: 125,768,426 (GRCm39)	L94F	probably benign	Het
Zbtb11	A	G	16: 55,826,383 (GRCm39)	K804R	probably damaging	Het
Zfp616	A	G	11: 73,974,894 (GRCm39)	R479G	probably benign	Het
Zfp661	T	C	2: 127,419,844 (GRCm39)	T99A	probably benign	Het
Zfp839	A	T	12: 110,821,532 (GRCm39)	Q115H	probably damaging	Het
Zyg11a	C	T	4: 108,046,765 (GRCm39)		probably null	Het

Other mutations in Oas3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00547:Oas3	APN	5	120,915,507 (GRCm39)	splice site	probably benign
IGL01095:Oas3	APN	5	120,910,954 (GRCm39)	missense	probably damaging	1.00
IGL01835:Oas3	APN	5	120,904,193 (GRCm39)	nonsense	probably null
IGL02006:Oas3	APN	5	120,907,300 (GRCm39)	missense	probably benign	0.00
IGL02811:Oas3	APN	5	120,902,387 (GRCm39)	missense	unknown
IGL03194:Oas3	APN	5	120,897,018 (GRCm39)	missense	probably damaging	1.00
R0066:Oas3	UTSW	5	120,896,940 (GRCm39)	missense	probably damaging	1.00
R0195:Oas3	UTSW	5	120,894,210 (GRCm39)	missense	probably damaging	1.00
R0196:Oas3	UTSW	5	120,894,210 (GRCm39)	missense	probably damaging	1.00
R0197:Oas3	UTSW	5	120,894,210 (GRCm39)	missense	probably damaging	1.00
R0445:Oas3	UTSW	5	120,894,210 (GRCm39)	missense	probably damaging	1.00
R0523:Oas3	UTSW	5	120,904,209 (GRCm39)	missense	unknown
R0592:Oas3	UTSW	5	120,909,214 (GRCm39)	missense	probably damaging	1.00
R0946:Oas3	UTSW	5	120,907,128 (GRCm39)	missense	unknown
R1354:Oas3	UTSW	5	120,908,065 (GRCm39)	missense	possibly damaging	0.94
R1642:Oas3	UTSW	5	120,915,639 (GRCm39)	missense	possibly damaging	0.90
R1681:Oas3	UTSW	5	120,907,973 (GRCm39)	missense	probably benign	0.22
R1844:Oas3	UTSW	5	120,898,045 (GRCm39)	missense	probably damaging	0.99
R1981:Oas3	UTSW	5	120,899,900 (GRCm39)	splice site	probably benign
R2443:Oas3	UTSW	5	120,915,553 (GRCm39)	missense	probably benign	0.35
R2902:Oas3	UTSW	5	120,896,982 (GRCm39)	missense	probably damaging	1.00
R3034:Oas3	UTSW	5	120,909,121 (GRCm39)	missense	probably damaging	1.00
R4565:Oas3	UTSW	5	120,909,104 (GRCm39)	missense	probably damaging	1.00
R4692:Oas3	UTSW	5	120,907,420 (GRCm39)	missense	probably benign	0.03
R4723:Oas3	UTSW	5	120,904,321 (GRCm39)	missense	unknown
R4812:Oas3	UTSW	5	120,899,212 (GRCm39)	unclassified	probably benign
R5288:Oas3	UTSW	5	120,895,055 (GRCm39)	missense	probably damaging	1.00
R5343:Oas3	UTSW	5	120,894,303 (GRCm39)	missense	possibly damaging	0.70
R5494:Oas3	UTSW	5	120,899,709 (GRCm39)	missense	unknown
R5688:Oas3	UTSW	5	120,896,867 (GRCm39)	missense	probably benign	0.31
R5894:Oas3	UTSW	5	120,895,019 (GRCm39)	missense	probably damaging	1.00
R5921:Oas3	UTSW	5	120,908,046 (GRCm39)	missense	probably damaging	1.00
R6037:Oas3	UTSW	5	120,907,384 (GRCm39)	missense	probably benign	0.41
R6037:Oas3	UTSW	5	120,907,384 (GRCm39)	missense	probably benign	0.41
R6066:Oas3	UTSW	5	120,910,989 (GRCm39)	missense	probably damaging	0.97
R6104:Oas3	UTSW	5	120,899,758 (GRCm39)	missense	unknown
R6134:Oas3	UTSW	5	120,907,113 (GRCm39)	missense	unknown
R6255:Oas3	UTSW	5	120,909,295 (GRCm39)	missense	probably benign	0.04
R6257:Oas3	UTSW	5	120,899,200 (GRCm39)	unclassified	probably benign
R6776:Oas3	UTSW	5	120,896,939 (GRCm39)	missense	probably damaging	1.00
R8137:Oas3	UTSW	5	120,915,565 (GRCm39)	missense	probably benign	0.07
R8967:Oas3	UTSW	5	120,896,907 (GRCm39)	missense	probably damaging	0.99
R9124:Oas3	UTSW	5	120,912,170 (GRCm39)	missense	probably damaging	1.00
R9287:Oas3	UTSW	5	120,892,754 (GRCm39)	missense	probably damaging	1.00
R9661:Oas3	UTSW	5	120,904,230 (GRCm39)	missense	unknown
R9745:Oas3	UTSW	5	120,899,284 (GRCm39)	missense	unknown
RF006:Oas3	UTSW	5	120,912,165 (GRCm39)	missense	probably damaging	1.00
X0024:Oas3	UTSW	5	120,899,793 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- GACCCGTGTCTCCTCTAATG -3'
(R):5'- GGGTCTCAAATCTCAAGGGACG -3'

Sequencing Primer
(F):5'- GAAATTTACTCAGGGCACAGTATCTG -3'
(R):5'- ACGCTAAGGGTCTCTCAGAC -3'

Posted On

2020-01-23