Mutagenetix > Incidental Mutation

Incidental Mutation 'R8023:Vmn2r16'

617522

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r16

Ensembl Gene

ENSMUSG00000092080

Gene Name

vomeronasal 2, receptor 16

Synonyms

EG384220

MMRRC Submission

067462-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.154) question?

Stock #

R8023 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

109478247-109512347 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to T at 109488272 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Stop codon at position 382 (Q382*)

Ref Sequence

ENSEMBL: ENSMUSP00000127838 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165180]

AlphaFold

A0A3B2WCY4

Predicted Effect

probably null
Transcript: ENSMUST00000165180
AA Change: Q382*

SMART Domains

Protein: ENSMUSP00000127838
Gene: ENSMUSG00000092080
AA Change: Q382*

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	76	425	2.3e-28	PFAM
Pfam:NCD3G	509	563	8.2e-19	PFAM
Pfam:7tm_3	596	831	3.5e-56	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsbg2	A	G	17: 57,152,448 (GRCm39)	Y665H	probably damaging	Het
Acte1	T	C	7: 143,445,528 (GRCm39)	S192P	probably damaging	Het
Agbl4	T	A	4: 111,474,345 (GRCm39)	V378E	probably benign	Het
Card9	T	C	2: 26,247,327 (GRCm39)	D274G	probably benign	Het
Ccnc	T	C	4: 21,747,578 (GRCm39)		probably null	Het
Col1a2	T	G	6: 4,533,847 (GRCm39)	S843A	unknown	Het
Cux1	T	A	5: 136,402,251 (GRCm39)	I111F	probably damaging	Het
Fam24b	A	C	7: 130,927,869 (GRCm39)	S107A	probably benign	Het
Fndc7	C	T	3: 108,774,461 (GRCm39)	C599Y	probably damaging	Het
Gm3604	G	A	13: 62,517,683 (GRCm39)	A225V	probably damaging	Het
Grhl3	A	G	4: 135,277,640 (GRCm39)	V475A	probably benign	Het
H2ac20	A	G	3: 96,128,106 (GRCm39)	S19P	unknown	Het
Heg1	T	A	16: 33,550,895 (GRCm39)	V958E	possibly damaging	Het
Hibch	T	C	1: 52,899,197 (GRCm39)	M30T	probably benign	Het
Hk2	T	A	6: 82,705,790 (GRCm39)	M838L	probably benign	Het
Hpd	A	G	5: 123,314,297 (GRCm39)	F206S	probably damaging	Het
Il34	C	T	8: 111,469,284 (GRCm39)	C177Y	probably damaging	Het
Il6ra	A	G	3: 89,820,260 (GRCm39)		probably null	Het
Itpr2	A	G	6: 146,088,988 (GRCm39)	I2240T	probably damaging	Het
Med24	A	G	11: 98,609,321 (GRCm39)		probably null	Het
Mpz	A	G	1: 170,987,602 (GRCm39)	D246G	probably damaging	Het
Ncam1	C	A	9: 49,421,057 (GRCm39)	A753S	probably benign	Het
Ndufs2	A	T	1: 171,064,263 (GRCm39)	M375K	probably damaging	Het
Obox2	A	G	7: 15,131,145 (GRCm39)	K84E	possibly damaging	Het
Or4c31	A	G	2: 88,292,022 (GRCm39)	I132V	probably benign	Het
Or51k1	A	G	7: 103,661,006 (GRCm39)	I301T	probably damaging	Het
Or7e178	C	T	9: 20,225,545 (GRCm39)	V224I	probably benign	Het
Pds5a	A	T	5: 65,795,241 (GRCm39)	L665Q	probably damaging	Het
Prr29	A	G	11: 106,267,099 (GRCm39)	E38G	probably benign	Het
Ptpn3	G	C	4: 57,248,688 (GRCm39)	D215E	probably benign	Het
Ptprq	G	A	10: 107,488,477 (GRCm39)	Q987*	probably null	Het
Ranbp6	T	C	19: 29,789,222 (GRCm39)	S377G	possibly damaging	Het
Rps6ka1	A	T	4: 133,594,506 (GRCm39)	L168Q	probably damaging	Het
Sall1	A	G	8: 89,759,171 (GRCm39)	I311T	probably damaging	Het
Satb2	T	C	1: 56,930,390 (GRCm39)	Y211C	probably damaging	Het
Sis	T	A	3: 72,859,813 (GRCm39)	Y314F	probably damaging	Het
Slc1a7	G	A	4: 107,869,473 (GRCm39)	V513M	probably benign	Het
Slc6a20a	T	C	9: 123,489,657 (GRCm39)	N129D	probably damaging	Het
Slu7	G	A	11: 43,336,975 (GRCm39)	R572Q	probably benign	Het
Tbx6	C	T	7: 126,382,031 (GRCm39)	A123V	possibly damaging	Het
Tcn2	A	T	11: 3,877,579 (GRCm39)	I23K	possibly damaging	Het
Tiparp	A	G	3: 65,439,224 (GRCm39)	D180G	probably benign	Het
Tln2	A	G	9: 67,131,346 (GRCm39)	L1400P	probably damaging	Het
Ttf2	T	C	3: 100,863,571 (GRCm39)	T588A	probably benign	Het
Ttn	A	G	2: 76,769,564 (GRCm39)	V2741A	unknown	Het
Txlna	A	T	4: 129,533,278 (GRCm39)	S83R	probably damaging	Het
Vmn2r76	A	G	7: 85,879,028 (GRCm39)	V424A	probably benign	Het
Zfc3h1	T	A	10: 115,256,553 (GRCm39)	L1508I	probably damaging	Het
Zfp189	G	A	4: 49,530,312 (GRCm39)	G472R	probably damaging	Het
Zfp462	G	A	4: 55,073,106 (GRCm39)		probably null	Het

Other mutations in Vmn2r16

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01116:Vmn2r16	APN	5	109,488,294 (GRCm39)	missense	probably damaging	1.00
IGL01374:Vmn2r16	APN	5	109,478,283 (GRCm39)	missense	probably benign	0.00
IGL01391:Vmn2r16	APN	5	109,511,627 (GRCm39)	missense	possibly damaging	0.50
IGL01419:Vmn2r16	APN	5	109,510,267 (GRCm39)	splice site	probably benign
IGL01634:Vmn2r16	APN	5	109,488,177 (GRCm39)	missense	probably benign	0.00
IGL01845:Vmn2r16	APN	5	109,511,762 (GRCm39)	missense	probably damaging	1.00
IGL01875:Vmn2r16	APN	5	109,478,277 (GRCm39)	missense	probably benign	0.01
IGL01910:Vmn2r16	APN	5	109,487,951 (GRCm39)	missense	probably damaging	1.00
IGL02217:Vmn2r16	APN	5	109,487,676 (GRCm39)	missense	probably damaging	0.98
IGL02327:Vmn2r16	APN	5	109,487,987 (GRCm39)	missense	probably benign	0.01
IGL02491:Vmn2r16	APN	5	109,487,703 (GRCm39)	nonsense	probably null
IGL02531:Vmn2r16	APN	5	109,488,134 (GRCm39)	missense	probably damaging	0.99
IGL02680:Vmn2r16	APN	5	109,487,948 (GRCm39)	missense	probably benign	0.44
IGL02884:Vmn2r16	APN	5	109,508,757 (GRCm39)	missense	possibly damaging	0.94
IGL03084:Vmn2r16	APN	5	109,478,292 (GRCm39)	missense	probably damaging	0.99
IGL03096:Vmn2r16	APN	5	109,487,751 (GRCm39)	missense	probably damaging	0.99
IGL03355:Vmn2r16	APN	5	109,511,580 (GRCm39)	missense	possibly damaging	0.74
R0280:Vmn2r16	UTSW	5	109,488,005 (GRCm39)	missense	possibly damaging	0.88
R0594:Vmn2r16	UTSW	5	109,511,762 (GRCm39)	missense	probably damaging	1.00
R1016:Vmn2r16	UTSW	5	109,487,754 (GRCm39)	missense	probably damaging	1.00
R1109:Vmn2r16	UTSW	5	109,487,652 (GRCm39)	missense	probably damaging	0.98
R1248:Vmn2r16	UTSW	5	109,508,643 (GRCm39)	missense	probably benign	0.10
R1626:Vmn2r16	UTSW	5	109,511,443 (GRCm39)	missense	probably damaging	1.00
R1909:Vmn2r16	UTSW	5	109,511,853 (GRCm39)	missense	probably benign	0.01
R1929:Vmn2r16	UTSW	5	109,487,124 (GRCm39)	missense	possibly damaging	0.92
R1982:Vmn2r16	UTSW	5	109,511,890 (GRCm39)	missense	probably benign	0.01
R3038:Vmn2r16	UTSW	5	109,487,199 (GRCm39)	missense	probably damaging	1.00
R3437:Vmn2r16	UTSW	5	109,478,362 (GRCm39)	missense	probably damaging	0.99
R3734:Vmn2r16	UTSW	5	109,478,280 (GRCm39)	missense	probably benign	0.11
R3820:Vmn2r16	UTSW	5	109,510,143 (GRCm39)	missense	probably benign	0.36
R3873:Vmn2r16	UTSW	5	109,488,177 (GRCm39)	missense	probably benign	0.33
R4165:Vmn2r16	UTSW	5	109,478,427 (GRCm39)	missense	possibly damaging	0.80
R4373:Vmn2r16	UTSW	5	109,511,667 (GRCm39)	missense	probably damaging	1.00
R4575:Vmn2r16	UTSW	5	109,511,665 (GRCm39)	missense	possibly damaging	0.81
R4576:Vmn2r16	UTSW	5	109,511,665 (GRCm39)	missense	possibly damaging	0.81
R4578:Vmn2r16	UTSW	5	109,511,665 (GRCm39)	missense	possibly damaging	0.81
R4637:Vmn2r16	UTSW	5	109,478,280 (GRCm39)	missense	probably benign	0.00
R4696:Vmn2r16	UTSW	5	109,487,168 (GRCm39)	missense	probably benign	0.01
R5026:Vmn2r16	UTSW	5	109,508,722 (GRCm39)	nonsense	probably null
R5180:Vmn2r16	UTSW	5	109,478,391 (GRCm39)	missense	probably benign	0.01
R5433:Vmn2r16	UTSW	5	109,511,708 (GRCm39)	missense	probably damaging	1.00
R5955:Vmn2r16	UTSW	5	109,511,613 (GRCm39)	missense	possibly damaging	0.78
R5958:Vmn2r16	UTSW	5	109,510,153 (GRCm39)	missense	possibly damaging	0.81
R6353:Vmn2r16	UTSW	5	109,488,119 (GRCm39)	missense	probably benign	0.33
R6389:Vmn2r16	UTSW	5	109,478,344 (GRCm39)	missense	probably benign	0.19
R6819:Vmn2r16	UTSW	5	109,488,412 (GRCm39)	missense	probably benign	0.04
R6994:Vmn2r16	UTSW	5	109,487,969 (GRCm39)	missense	probably damaging	1.00
R7061:Vmn2r16	UTSW	5	109,511,620 (GRCm39)	missense	probably damaging	0.99
R7063:Vmn2r16	UTSW	5	109,511,650 (GRCm39)	missense	probably damaging	1.00
R7220:Vmn2r16	UTSW	5	109,508,772 (GRCm39)	missense	probably damaging	0.97
R7268:Vmn2r16	UTSW	5	109,488,331 (GRCm39)	nonsense	probably null
R7420:Vmn2r16	UTSW	5	109,511,736 (GRCm39)	missense	probably damaging	0.96
R7591:Vmn2r16	UTSW	5	109,510,223 (GRCm39)	missense	probably damaging	0.99
R7644:Vmn2r16	UTSW	5	109,487,837 (GRCm39)	missense	probably damaging	1.00
R7939:Vmn2r16	UTSW	5	109,487,705 (GRCm39)	missense	possibly damaging	0.79
R7977:Vmn2r16	UTSW	5	109,488,015 (GRCm39)	missense	probably damaging	1.00
R7987:Vmn2r16	UTSW	5	109,488,015 (GRCm39)	missense	probably damaging	1.00
R8427:Vmn2r16	UTSW	5	109,488,138 (GRCm39)	missense	probably benign	0.03
R8436:Vmn2r16	UTSW	5	109,511,649 (GRCm39)	missense	probably damaging	1.00
R8475:Vmn2r16	UTSW	5	109,487,073 (GRCm39)	missense	probably benign	0.01
R8554:Vmn2r16	UTSW	5	109,511,997 (GRCm39)	missense	probably benign	0.13
R8771:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8774:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8774-TAIL:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8775:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8775-TAIL:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8777:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8777-TAIL:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8825:Vmn2r16	UTSW	5	109,487,019 (GRCm39)	missense	probably benign	0.41
R8865:Vmn2r16	UTSW	5	109,487,910 (GRCm39)	missense	probably benign	0.33
R8900:Vmn2r16	UTSW	5	109,511,619 (GRCm39)	missense	probably benign	0.04
R9365:Vmn2r16	UTSW	5	109,488,064 (GRCm39)	missense	probably damaging	0.99
R9683:Vmn2r16	UTSW	5	109,511,677 (GRCm39)	missense	probably damaging	1.00
X0027:Vmn2r16	UTSW	5	109,487,175 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r16	UTSW	5	109,511,779 (GRCm39)	frame shift	probably null
Z1088:Vmn2r16	UTSW	5	109,488,381 (GRCm39)	missense	probably benign	0.03
Z1177:Vmn2r16	UTSW	5	109,487,864 (GRCm39)	missense	possibly damaging	0.79

Predicted Primers

PCR Primer
(F):5'- CATTTGCCCACCACAATGG -3'
(R):5'- CTCTGTGGCCATTTGAAATATCAC -3'

Sequencing Primer
(F):5'- GCCCACCACAATGGCAGAATTG -3'
(R):5'- GTGGCCATTTGAAATATCACAATGAC -3'

Posted On

2020-01-23