Mutagenetix > Incidental Mutation

Incidental Mutation 'R8030:Helz2'

617876

Institutional Source

Beutler Lab

Gene Symbol

Helz2

Ensembl Gene

ENSMUSG00000027580

Gene Name

helicase with zinc finger 2, transcriptional coactivator

Synonyms

BC006779

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8030 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

181227615-181242027 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 181237896 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Tyrosine at position 643 (F643Y)

Ref Sequence

ENSEMBL: ENSMUSP00000091756 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094203] [ENSMUST00000108831] [ENSMUST00000121484]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000094203
AA Change: F643Y

PolyPhen 2 Score 0.546 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000091756
Gene: ENSMUSG00000027580
AA Change: F643Y

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
AAA	782	973	1.41e-2	SMART
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
AAA	2462	2713	1.48e0	SMART
SCOP:d1pjr_2	2793	2838	2e-6	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000108831
AA Change: F643Y

PolyPhen 2 Score 0.608 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000104459
Gene: ENSMUSG00000027580
AA Change: F643Y

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
AAA	782	973	1.41e-2	SMART
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
AAA	2462	2713	1.48e0	SMART
SCOP:d1pjr_2	2793	2838	2e-6	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000121484
AA Change: F643Y

PolyPhen 2 Score 0.621 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000112917
Gene: ENSMUSG00000027580
AA Change: F643Y

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
Pfam:AAA_11	761	877	3.9e-10	PFAM
Pfam:AAA_19	780	849	1.7e-7	PFAM
Pfam:AAA_11	870	952	2e-15	PFAM
Pfam:AAA_12	958	1162	3.8e-26	PFAM
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
Pfam:AAA_11	2400	2653	4e-42	PFAM
Pfam:AAA_12	2660	2866	2e-47	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a nuclear transcriptional co-activator for peroxisome proliferator activated receptor alpha. The encoded protein contains a zinc finger and is a helicase that appears to be part of the peroxisome proliferator activated receptor alpha interacting complex. This gene is a member of the DNA2/NAM7 helicase gene family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit slower weight gain, hyperleptinemia, increased oxygen consumption, decreased respiratory quotient, decreased liver triglyceride level and ameliorated hyperlipidemia and hepatosteatosis when fed a high-fat diet. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700012B09Rik	A	G	9: 14,761,674 (GRCm38)	S98P	probably benign	Het
2410089E03Rik	G	T	15: 8,230,303 (GRCm38)	G2383V	probably damaging	Het
Abca9	C	T	11: 110,120,708 (GRCm38)	V1170I	probably benign	Het
Acacb	A	G	5: 114,233,167 (GRCm38)	T1786A	probably damaging	Het
Acmsd	T	C	1: 127,749,161 (GRCm38)	I141T	possibly damaging	Het
Akr1c12	A	G	13: 4,272,245 (GRCm38)	V266A	possibly damaging	Het
Arhgef18	A	T	8: 3,439,600 (GRCm38)	I311F	probably damaging	Het
Armc2	T	A	10: 41,966,742 (GRCm38)	N355I	possibly damaging	Het
Armh1	T	A	4: 117,229,987 (GRCm38)	K160N	probably benign	Het
Asic1	A	T	15: 99,694,841 (GRCm38)	T236S	possibly damaging	Het
Avl9	T	A	6: 56,741,422 (GRCm38)	D424E	probably damaging	Het
Cbfa2t2	A	T	2: 154,515,896 (GRCm38)	Q197L	probably damaging	Het
Ccdc136	T	C	6: 29,417,142 (GRCm38)	V654A	probably benign	Het
Ccdc155	CGGCTCAGGCTCAGGCTCAGGCTCAGGCTCAGGCTCAGGCTCAGGCTC	CGGCTCAGGCTCAGGCTCAGGCTCAGGCTCAGGCTCAGGCTCAGGCTCAGGCTC	7: 45,188,184 (GRCm38)		probably benign	Het
Cd177	C	T	7: 24,756,169 (GRCm38)	W309*	probably null	Het
Cracr2a	A	G	6: 127,611,423 (GRCm38)	K182E	probably damaging	Het
Dpys	T	C	15: 39,828,090 (GRCm38)	T279A	possibly damaging	Het
Dsc1	A	T	18: 20,089,571 (GRCm38)	S615T	probably benign	Het
Dsc2	C	T	18: 20,032,274 (GRCm38)	G881R	possibly damaging	Het
Efcab14	A	G	4: 115,766,402 (GRCm38)	Q390R	probably benign	Het
Eif4ebp2	G	A	10: 61,435,046 (GRCm38)	A68V	probably damaging	Het
Fam81a	A	G	9: 70,102,909 (GRCm38)	S149P	probably benign	Het
Ffar4	A	G	19: 38,107,391 (GRCm38)	I193V	possibly damaging	Het
Flvcr2	T	A	12: 85,798,538 (GRCm38)	V377D	probably damaging	Het
Fscn1	C	T	5: 142,961,001 (GRCm38)	R185C	possibly damaging	Het
Gucy1b2	C	T	14: 62,392,870 (GRCm38)	S809N	probably benign	Het
H60b	T	A	10: 22,287,121 (GRCm38)	N198K	probably damaging	Het
Kif28	A	G	1: 179,699,064 (GRCm38)	V846A	probably benign	Het
Kirrel	C	A	3: 87,097,775 (GRCm38)	G89W	probably damaging	Het
Krt42	G	C	11: 100,265,039 (GRCm38)	R294G	possibly damaging	Het
Mb21d2	A	G	16: 28,827,803 (GRCm38)	F473S	probably damaging	Het
Mcrip2	G	A	17: 25,864,332 (GRCm38)	Q111*	probably null	Het
Msh5	A	G	17: 35,029,748 (GRCm38)	Y741H	possibly damaging	Het
Myo7b	A	G	18: 31,998,082 (GRCm38)	I544T	probably damaging	Het
Nav1	T	C	1: 135,537,239 (GRCm38)	E276G	probably damaging	Het
Nr2f1	T	C	13: 78,195,446 (GRCm38)	N233S	probably benign	Het
Nrip2	A	T	6: 128,406,521 (GRCm38)	D124V	possibly damaging	Het
Olfr1008	T	A	2: 85,689,719 (GRCm38)	C97S	probably damaging	Het
Olfr1042	C	T	2: 86,160,242 (GRCm38)	V43I	probably benign	Het
Panx2	G	T	15: 89,068,079 (GRCm38)	A250S	probably damaging	Het
Pdc	T	C	1: 150,333,213 (GRCm38)	L149P	probably damaging	Het
Pex5l	T	A	3: 32,954,419 (GRCm38)	I445F	possibly damaging	Het
Pigc	T	C	1: 161,970,547 (GRCm38)	F33L	probably damaging	Het
Pkp2	A	T	16: 16,246,910 (GRCm38)	M433L	probably benign	Het
Rbfox2	A	G	15: 77,085,576 (GRCm38)		probably null	Het
Rd3l	A	G	12: 111,980,150 (GRCm38)	L64P	possibly damaging	Het
Rnf220	A	G	4: 117,277,828 (GRCm38)	Y409H	probably damaging	Het
Rsf1	G	GACGGCGGCC	7: 97,579,909 (GRCm38)		probably benign	Het
Sel1l2	T	C	2: 140,241,018 (GRCm38)	T567A	probably damaging	Het
Slc22a8	T	C	19: 8,610,007 (GRCm38)	I477T	probably damaging	Het
Sltm	C	T	9: 70,585,979 (GRCm38)	R753*	probably null	Het
Specc1l	T	A	10: 75,248,555 (GRCm38)	M687K	probably damaging	Het
Spock3	T	G	8: 63,352,198 (GRCm38)	C338G	probably damaging	Het
Ssh2	C	A	11: 77,454,506 (GRCm38)	Q1106K	probably benign	Het
Sycp2l	A	G	13: 41,172,670 (GRCm38)	M251V	not run	Het
Tdrd5	C	A	1: 156,270,595 (GRCm38)	E711*	probably null	Het
Thop1	T	C	10: 81,075,616 (GRCm38)	M112T	possibly damaging	Het
Tln1	C	T	4: 43,535,737 (GRCm38)		probably null	Het
Ttc28	A	C	5: 111,286,056 (GRCm38)	I2319L	possibly damaging	Het
Ttll11	A	G	2: 35,902,673 (GRCm38)	I386T	probably damaging	Het
Txndc8	T	C	4: 57,984,178 (GRCm38)	E151G	probably damaging	Het
Ubr1	T	C	2: 120,934,374 (GRCm38)	E533G	probably damaging	Het
Zscan4f	A	G	7: 11,401,363 (GRCm38)	H232R	probably benign	Het

Other mutations in Helz2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Helz2	APN	2	181,229,702 (GRCm38)	missense	probably damaging	1.00
IGL00515:Helz2	APN	2	181,233,006 (GRCm38)	nonsense	probably null
IGL00704:Helz2	APN	2	181,234,385 (GRCm38)	missense	probably damaging	1.00
IGL00847:Helz2	APN	2	181,232,245 (GRCm38)	missense	possibly damaging	0.73
IGL01448:Helz2	APN	2	181,233,977 (GRCm38)	missense	probably damaging	1.00
IGL01783:Helz2	APN	2	181,232,881 (GRCm38)	missense	probably damaging	1.00
IGL01790:Helz2	APN	2	181,238,481 (GRCm38)	missense	probably benign	0.29
IGL02116:Helz2	APN	2	181,232,185 (GRCm38)	missense	probably damaging	1.00
IGL02226:Helz2	APN	2	181,231,690 (GRCm38)	missense	probably damaging	1.00
IGL02402:Helz2	APN	2	181,230,911 (GRCm38)	missense	probably damaging	1.00
IGL02403:Helz2	APN	2	181,231,022 (GRCm38)	missense	probably damaging	1.00
IGL02733:Helz2	APN	2	181,235,026 (GRCm38)	missense	probably benign	0.14
IGL02869:Helz2	APN	2	181,231,146 (GRCm38)	intron	probably benign
IGL03003:Helz2	APN	2	181,240,253 (GRCm38)	missense	probably damaging	1.00
IGL03060:Helz2	APN	2	181,229,222 (GRCm38)	critical splice donor site	probably null
IGL03310:Helz2	APN	2	181,231,804 (GRCm38)	missense	probably benign	0.00
Colby	UTSW	2	181,233,202 (GRCm38)	missense	probably damaging	1.00
ANU74:Helz2	UTSW	2	181,234,834 (GRCm38)	missense	probably benign	0.03
R0013:Helz2	UTSW	2	181,240,959 (GRCm38)	missense	probably benign
R0013:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0014:Helz2	UTSW	2	181,240,511 (GRCm38)	missense	probably damaging	1.00
R0014:Helz2	UTSW	2	181,240,511 (GRCm38)	missense	probably damaging	1.00
R0016:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0018:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0019:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0019:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0055:Helz2	UTSW	2	181,228,821 (GRCm38)	missense	possibly damaging	0.47
R0055:Helz2	UTSW	2	181,228,821 (GRCm38)	missense	possibly damaging	0.47
R0071:Helz2	UTSW	2	181,236,407 (GRCm38)	missense	probably damaging	1.00
R0071:Helz2	UTSW	2	181,236,407 (GRCm38)	missense	probably damaging	1.00
R0111:Helz2	UTSW	2	181,237,802 (GRCm38)	missense	probably benign	0.30
R0117:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0135:Helz2	UTSW	2	181,232,269 (GRCm38)	missense	probably damaging	1.00
R0194:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0242:Helz2	UTSW	2	181,230,430 (GRCm38)	missense	probably damaging	1.00
R0242:Helz2	UTSW	2	181,230,430 (GRCm38)	missense	probably damaging	1.00
R0254:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0410:Helz2	UTSW	2	181,230,593 (GRCm38)	missense	probably damaging	1.00
R0442:Helz2	UTSW	2	181,232,209 (GRCm38)	missense	probably damaging	0.97
R0497:Helz2	UTSW	2	181,229,656 (GRCm38)	missense	probably damaging	0.97
R0517:Helz2	UTSW	2	181,227,770 (GRCm38)	missense	probably benign	0.00
R0541:Helz2	UTSW	2	181,234,825 (GRCm38)	missense	possibly damaging	0.89
R0542:Helz2	UTSW	2	181,232,089 (GRCm38)	missense	probably damaging	1.00
R0591:Helz2	UTSW	2	181,232,116 (GRCm38)	missense	probably damaging	0.96
R0692:Helz2	UTSW	2	181,240,881 (GRCm38)	missense	probably benign
R0826:Helz2	UTSW	2	181,240,853 (GRCm38)	missense	possibly damaging	0.51
R0834:Helz2	UTSW	2	181,230,777 (GRCm38)	missense	probably damaging	1.00
R0880:Helz2	UTSW	2	181,236,135 (GRCm38)	missense	probably benign
R1170:Helz2	UTSW	2	181,229,815 (GRCm38)	missense	probably damaging	1.00
R1186:Helz2	UTSW	2	181,231,128 (GRCm38)	missense	probably damaging	1.00
R1344:Helz2	UTSW	2	181,237,596 (GRCm38)	missense	possibly damaging	0.89
R1358:Helz2	UTSW	2	181,232,981 (GRCm38)	missense	probably damaging	1.00
R1436:Helz2	UTSW	2	181,235,524 (GRCm38)	missense	probably damaging	0.99
R1464:Helz2	UTSW	2	181,239,654 (GRCm38)	missense	probably damaging	1.00
R1464:Helz2	UTSW	2	181,239,654 (GRCm38)	missense	probably damaging	1.00
R1466:Helz2	UTSW	2	181,236,297 (GRCm38)	missense	probably damaging	1.00
R1466:Helz2	UTSW	2	181,236,297 (GRCm38)	missense	probably damaging	1.00
R1477:Helz2	UTSW	2	181,232,804 (GRCm38)	missense	probably benign	0.00
R1564:Helz2	UTSW	2	181,233,228 (GRCm38)	missense	probably benign	0.01
R1584:Helz2	UTSW	2	181,236,297 (GRCm38)	missense	probably damaging	1.00
R1655:Helz2	UTSW	2	181,234,147 (GRCm38)	missense	probably damaging	0.99
R1757:Helz2	UTSW	2	181,236,263 (GRCm38)	missense	probably damaging	1.00
R1779:Helz2	UTSW	2	181,238,459 (GRCm38)	missense	possibly damaging	0.84
R1779:Helz2	UTSW	2	181,234,987 (GRCm38)	missense	probably benign
R1837:Helz2	UTSW	2	181,229,289 (GRCm38)	missense	probably damaging	1.00
R1845:Helz2	UTSW	2	181,232,085 (GRCm38)	missense	probably benign	0.02
R1894:Helz2	UTSW	2	181,234,289 (GRCm38)	missense	probably damaging	1.00
R1913:Helz2	UTSW	2	181,233,750 (GRCm38)	missense	probably damaging	1.00
R2005:Helz2	UTSW	2	181,231,329 (GRCm38)	missense	probably benign	0.45
R2034:Helz2	UTSW	2	181,232,578 (GRCm38)	missense	probably damaging	1.00
R2036:Helz2	UTSW	2	181,237,479 (GRCm38)	missense	probably benign	0.03
R2061:Helz2	UTSW	2	181,240,544 (GRCm38)	missense	probably damaging	1.00
R2088:Helz2	UTSW	2	181,235,102 (GRCm38)	missense	probably benign	0.07
R2142:Helz2	UTSW	2	181,231,380 (GRCm38)	missense	probably benign
R2180:Helz2	UTSW	2	181,233,732 (GRCm38)	missense	probably damaging	1.00
R2192:Helz2	UTSW	2	181,229,048 (GRCm38)	nonsense	probably null
R2248:Helz2	UTSW	2	181,233,433 (GRCm38)	missense	probably benign	0.33
R2495:Helz2	UTSW	2	181,232,912 (GRCm38)	missense	probably damaging	0.99
R2886:Helz2	UTSW	2	181,240,742 (GRCm38)	missense	probably benign
R3617:Helz2	UTSW	2	181,233,061 (GRCm38)	missense	probably damaging	1.00
R3776:Helz2	UTSW	2	181,240,389 (GRCm38)	nonsense	probably null
R3803:Helz2	UTSW	2	181,239,996 (GRCm38)	missense	probably damaging	0.96
R4043:Helz2	UTSW	2	181,229,710 (GRCm38)	missense	probably benign	0.00
R4052:Helz2	UTSW	2	181,240,475 (GRCm38)	missense	probably damaging	1.00
R4232:Helz2	UTSW	2	181,229,902 (GRCm38)	missense	probably damaging	1.00
R4521:Helz2	UTSW	2	181,228,833 (GRCm38)	missense	probably benign
R4624:Helz2	UTSW	2	181,239,308 (GRCm38)	missense	probably damaging	0.99
R4720:Helz2	UTSW	2	181,238,417 (GRCm38)	missense	probably damaging	1.00
R4831:Helz2	UTSW	2	181,237,417 (GRCm38)	missense	probably damaging	1.00
R4852:Helz2	UTSW	2	181,230,120 (GRCm38)	missense	probably damaging	1.00
R4894:Helz2	UTSW	2	181,236,147 (GRCm38)	missense	probably benign	0.01
R4915:Helz2	UTSW	2	181,232,438 (GRCm38)	missense	possibly damaging	0.80
R4965:Helz2	UTSW	2	181,240,916 (GRCm38)	missense	possibly damaging	0.79
R5022:Helz2	UTSW	2	181,240,569 (GRCm38)	missense	probably benign
R5089:Helz2	UTSW	2	181,235,149 (GRCm38)	missense	probably benign	0.14
R5190:Helz2	UTSW	2	181,230,757 (GRCm38)	critical splice donor site	probably null
R5309:Helz2	UTSW	2	181,234,846 (GRCm38)	missense	probably benign	0.08
R5358:Helz2	UTSW	2	181,235,528 (GRCm38)	missense	probably damaging	1.00
R5379:Helz2	UTSW	2	181,235,069 (GRCm38)	missense	probably benign
R5559:Helz2	UTSW	2	181,230,126 (GRCm38)	missense	probably damaging	0.98
R5591:Helz2	UTSW	2	181,240,258 (GRCm38)	missense	probably damaging	0.99
R5596:Helz2	UTSW	2	181,237,289 (GRCm38)	intron	probably benign
R5805:Helz2	UTSW	2	181,240,508 (GRCm38)	missense	probably damaging	1.00
R5823:Helz2	UTSW	2	181,236,396 (GRCm38)	missense	possibly damaging	0.92
R5825:Helz2	UTSW	2	181,232,656 (GRCm38)	missense	probably benign	0.02
R5873:Helz2	UTSW	2	181,234,028 (GRCm38)	missense	possibly damaging	0.78
R5928:Helz2	UTSW	2	181,230,384 (GRCm38)	missense	possibly damaging	0.82
R5936:Helz2	UTSW	2	181,230,767 (GRCm38)	missense	probably damaging	1.00
R5975:Helz2	UTSW	2	181,231,050 (GRCm38)	missense	probably benign	0.08
R6045:Helz2	UTSW	2	181,240,313 (GRCm38)	missense	probably benign	0.03
R6077:Helz2	UTSW	2	181,233,038 (GRCm38)	missense	probably benign	0.41
R6218:Helz2	UTSW	2	181,232,294 (GRCm38)	missense	probably benign	0.03
R6218:Helz2	UTSW	2	181,235,945 (GRCm38)	missense	probably damaging	1.00
R6315:Helz2	UTSW	2	181,233,202 (GRCm38)	missense	probably damaging	1.00
R6346:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6371:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6372:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6373:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6385:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6464:Helz2	UTSW	2	181,235,069 (GRCm38)	missense	probably benign
R6581:Helz2	UTSW	2	181,229,379 (GRCm38)	missense	probably damaging	0.99
R6651:Helz2	UTSW	2	181,239,557 (GRCm38)	nonsense	probably null
R6964:Helz2	UTSW	2	181,230,428 (GRCm38)	missense	probably damaging	1.00
R7061:Helz2	UTSW	2	181,240,514 (GRCm38)	missense	probably damaging	1.00
R7153:Helz2	UTSW	2	181,231,285 (GRCm38)	missense	probably benign	0.00
R7372:Helz2	UTSW	2	181,238,423 (GRCm38)	missense	possibly damaging	0.61
R7512:Helz2	UTSW	2	181,235,600 (GRCm38)	splice site	probably null
R7512:Helz2	UTSW	2	181,230,854 (GRCm38)	missense	probably benign	0.00
R7583:Helz2	UTSW	2	181,237,572 (GRCm38)	missense	probably benign	0.06
R7724:Helz2	UTSW	2	181,231,996 (GRCm38)	missense	probably damaging	1.00
R7733:Helz2	UTSW	2	181,230,355 (GRCm38)	missense	possibly damaging	0.63
R7748:Helz2	UTSW	2	181,234,531 (GRCm38)	missense	probably damaging	1.00
R7774:Helz2	UTSW	2	181,233,991 (GRCm38)	missense	probably benign
R7799:Helz2	UTSW	2	181,237,989 (GRCm38)	missense	probably benign	0.15
R7841:Helz2	UTSW	2	181,232,902 (GRCm38)	missense	probably damaging	1.00
R7939:Helz2	UTSW	2	181,237,750 (GRCm38)	missense	probably damaging	0.99
R8026:Helz2	UTSW	2	181,240,205 (GRCm38)	missense	probably benign	0.34
R8080:Helz2	UTSW	2	181,238,262 (GRCm38)	missense	probably damaging	0.99
R8237:Helz2	UTSW	2	181,229,331 (GRCm38)	missense	possibly damaging	0.65
R8245:Helz2	UTSW	2	181,238,102 (GRCm38)	missense	probably damaging	1.00
R8304:Helz2	UTSW	2	181,230,157 (GRCm38)	missense	probably benign	0.03
R8486:Helz2	UTSW	2	181,229,331 (GRCm38)	missense	probably damaging	1.00
R8556:Helz2	UTSW	2	181,229,557 (GRCm38)	missense	probably damaging	1.00
R8878:Helz2	UTSW	2	181,232,767 (GRCm38)	missense	possibly damaging	0.67
R8907:Helz2	UTSW	2	181,233,127 (GRCm38)	missense	possibly damaging	0.47
R8911:Helz2	UTSW	2	181,238,380 (GRCm38)	missense
R8953:Helz2	UTSW	2	181,233,091 (GRCm38)	missense	probably damaging	1.00
R8963:Helz2	UTSW	2	181,229,614 (GRCm38)	missense	probably damaging	1.00
R8969:Helz2	UTSW	2	181,237,788 (GRCm38)	missense	probably benign	0.19
R8976:Helz2	UTSW	2	181,234,693 (GRCm38)	missense	possibly damaging	0.46
R9015:Helz2	UTSW	2	181,228,999 (GRCm38)	missense	probably damaging	1.00
R9031:Helz2	UTSW	2	181,232,468 (GRCm38)	missense	possibly damaging	0.78
R9052:Helz2	UTSW	2	181,240,175 (GRCm38)	missense	possibly damaging	0.78
R9089:Helz2	UTSW	2	181,239,640 (GRCm38)	missense	probably damaging	1.00
R9145:Helz2	UTSW	2	181,240,055 (GRCm38)	missense	probably damaging	1.00
R9185:Helz2	UTSW	2	181,230,090 (GRCm38)	missense	probably benign
R9186:Helz2	UTSW	2	181,234,664 (GRCm38)	missense	possibly damaging	0.57
R9373:Helz2	UTSW	2	181,240,948 (GRCm38)	missense	probably benign
R9407:Helz2	UTSW	2	181,240,182 (GRCm38)	missense	probably benign	0.01
R9465:Helz2	UTSW	2	181,232,917 (GRCm38)	missense	probably benign	0.01
R9502:Helz2	UTSW	2	181,236,452 (GRCm38)	missense	possibly damaging	0.47
R9538:Helz2	UTSW	2	181,240,221 (GRCm38)	missense	probably damaging	1.00
R9554:Helz2	UTSW	2	181,240,677 (GRCm38)	missense	probably damaging	0.96
R9659:Helz2	UTSW	2	181,240,232 (GRCm38)	missense	probably benign	0.00
R9800:Helz2	UTSW	2	181,240,823 (GRCm38)	missense	probably damaging	0.99
X0064:Helz2	UTSW	2	181,231,741 (GRCm38)	missense	probably damaging	1.00
Z1176:Helz2	UTSW	2	181,237,564 (GRCm38)	missense	probably benign	0.39
Z1177:Helz2	UTSW	2	181,235,961 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AACTGCACCTCCAAGATGGG -3'
(R):5'- GCTAGCAGGAGTGGCTAAAGTC -3'

Sequencing Primer
(F):5'- CCTCCAAGATGGGGCTGTCTTG -3'
(R):5'- CTGGACTTAGAGATAGGTCTTTGAC -3'

Posted On

2020-01-23