|Institutional Source||Beutler Lab|
|Gene Name||eukaryotic translation initiation factor 4A3|
|Is this an essential gene?||Probably essential (E-score: 0.957)|
|Stock #||R8031 (G1)|
|Chromosomal Location||119288363-119300089 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to C at 119288905 bp (GRCm38)|
|Amino Acid Change||Tyrosine to Cysteine at position 352 (Y352C)|
|Ref Sequence||ENSEMBL: ENSMUSP00000026667 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000026666] [ENSMUST00000026667] [ENSMUST00000106253] [ENSMUST00000106259]|
AA Change: Y352C
PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
AA Change: Y352C
|Meta Mutation Damage Score||0.9628|
|Coding Region Coverage||
|Validation Efficiency||100% (60/60)|
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family. [provided by RefSeq, Jul 2008]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Eif4a3||
(F):5'- CCCTGAGAATCCGGATGTCATC -3'
(R):5'- GTTTTCCATCATGGCAGCAGG -3'
(F):5'- GAGAATCCGGATGTCATCATTCTTC -3'
(R):5'- CCATCATGGCAGCAGGTTTTG -3'