Incidental Mutation 'R8037:Ccr8'
ID 618297
Institutional Source Beutler Lab
Gene Symbol Ccr8
Ensembl Gene ENSMUSG00000042262
Gene Name C-C motif chemokine receptor 8
Synonyms Cmkbr8
MMRRC Submission 067474-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R8037 (G1)
Quality Score 225.009
Status Not validated
Chromosome 9
Chromosomal Location 119921199-119923972 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 119923436 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Phenylalanine to Leucine at position 184 (F184L)
Ref Sequence ENSEMBL: ENSMUSP00000038473 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000048777]
AlphaFold P56484
Predicted Effect probably benign
Transcript: ENSMUST00000048777
AA Change: F184L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000038473
Gene: ENSMUSG00000042262
AA Change: F184L

Pfam:7TM_GPCR_Srsx 44 313 2.4e-8 PFAM
Pfam:7tm_1 50 298 3.3e-44 PFAM
low complexity region 338 347 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.8%
  • 20x: 99.3%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are important for the migration of various cell types into the inflammatory sites. This receptor protein preferentially expresses in the thymus. I-309, thymus activation-regulated cytokine (TARC) and macrophage inflammatory protein-1 beta (MIP-1 beta) have been identified as ligands of this receptor. Studies of this receptor and its ligands suggested its role in regulation of monocyte chemotaxis and thymic cell apoptosis. More specifically, this receptor may contribute to the proper positioning of activated T cells within the antigenic challenge sites and specialized areas of lymphoid tissues. This gene is located at the chemokine receptor gene cluster region. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for either of two independently generated knock-out alleles show normal lung eosinophilia and Th2 cytokine responses in OVA-elicited asthma models. Mice homozygous for a third knock-out allele show a delay in onset of clinical signs of experimental autoimmune encephalomyelitis. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 47 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca13 G T 11: 9,243,904 (GRCm39) W1922C probably damaging Het
Acad11 T A 9: 103,953,035 (GRCm39) I88N possibly damaging Het
Adgrf3 A T 5: 30,404,510 (GRCm39) C309S probably damaging Het
Ankhd1 T A 18: 36,771,676 (GRCm39) V1343E probably damaging Het
Aqr A T 2: 113,992,161 (GRCm39) Y72N probably damaging Het
Bptf T C 11: 106,946,776 (GRCm39) T2339A probably damaging Het
Ccdc121rt3 A G 5: 112,502,882 (GRCm39) V274A probably benign Het
Dbh A G 2: 27,055,700 (GRCm39) D58G probably damaging Het
Depdc5 T C 5: 33,116,692 (GRCm39) probably null Het
Dhdds G A 4: 133,724,158 (GRCm39) T52I probably benign Het
Dnmt1 A C 9: 20,852,860 (GRCm39) V82G probably damaging Het
Efemp2 C T 19: 5,530,141 (GRCm39) Q290* probably null Het
Eipr1 A G 12: 28,914,676 (GRCm39) S277G probably benign Het
Fam110b A G 4: 5,799,511 (GRCm39) I310V possibly damaging Het
Foxn4 T C 5: 114,394,658 (GRCm39) D423G probably damaging Het
Fsip2 T A 2: 82,816,322 (GRCm39) N4018K possibly damaging Het
Gm12887 T A 4: 121,472,887 (GRCm39) D85V probably damaging Het
Gys2 A G 6: 142,394,119 (GRCm39) V473A probably benign Het
Hnrnpu A T 1: 178,159,917 (GRCm39) F388Y unknown Het
Hoxa5 A G 6: 52,181,309 (GRCm39) S8P probably damaging Het
Ier5 A T 1: 154,975,175 (GRCm39) M1K probably null Het
Ighv1-52 A G 12: 115,109,210 (GRCm39) F83S probably damaging Het
Lrch1 T C 14: 75,023,794 (GRCm39) D577G probably damaging Het
Lrrk1 A T 7: 65,935,089 (GRCm39) M1010K probably benign Het
Mttp T G 3: 137,796,883 (GRCm39) N873T probably damaging Het
Nphs2 A G 1: 156,138,400 (GRCm39) R15G possibly damaging Het
Or4a70 A T 2: 89,324,055 (GRCm39) N200K possibly damaging Het
Or5d16 T C 2: 87,773,319 (GRCm39) T218A probably benign Het
Or8d6 T A 9: 39,854,177 (GRCm39) V207D probably damaging Het
Pcdhga8 T C 18: 37,860,071 (GRCm39) S376P probably damaging Het
Pde3a A G 6: 141,429,650 (GRCm39) N737S possibly damaging Het
Pgap6 T C 17: 26,336,509 (GRCm39) L209P possibly damaging Het
Pira1 C G 7: 3,740,319 (GRCm39) A301P probably damaging Het
Rin1 T A 19: 5,101,852 (GRCm39) L179Q probably damaging Het
Sec63 T A 10: 42,659,483 (GRCm39) M57K probably benign Het
Senp2 C T 16: 21,832,888 (GRCm39) Q59* probably null Het
St8sia5 G A 18: 77,336,238 (GRCm39) V224I possibly damaging Het
Sycp2 A T 2: 178,045,571 (GRCm39) D16E probably damaging Het
Tab1 A G 15: 80,044,471 (GRCm39) T500A probably benign Het
Tead1 A G 7: 112,358,727 (GRCm39) D13G possibly damaging Het
Tecpr2 T C 12: 110,902,854 (GRCm39) F860L probably benign Het
Tg A G 15: 66,560,724 (GRCm39) M1029V probably benign Het
Tnni2 A G 7: 141,997,691 (GRCm39) R109G probably damaging Het
Unc79 A G 12: 103,016,178 (GRCm39) K320R probably damaging Het
Vmn1r9 T A 6: 57,047,988 (GRCm39) V21D probably benign Het
Zfp64 A G 2: 168,741,932 (GRCm39) F332S probably damaging Het
Other mutations in Ccr8
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01511:Ccr8 APN 9 119,923,691 (GRCm39) missense probably damaging 1.00
IGL02558:Ccr8 APN 9 119,923,724 (GRCm39) missense probably benign 0.01
IGL02966:Ccr8 APN 9 119,923,206 (GRCm39) missense probably damaging 0.96
IGL03135:Ccr8 APN 9 119,923,689 (GRCm39) missense possibly damaging 0.89
R0402:Ccr8 UTSW 9 119,923,976 (GRCm39) splice site probably null
R0739:Ccr8 UTSW 9 119,923,415 (GRCm39) missense probably damaging 1.00
R1069:Ccr8 UTSW 9 119,923,283 (GRCm39) missense probably benign 0.00
R4766:Ccr8 UTSW 9 119,923,530 (GRCm39) missense probably damaging 1.00
R4934:Ccr8 UTSW 9 119,923,815 (GRCm39) missense probably benign
R5116:Ccr8 UTSW 9 119,923,095 (GRCm39) missense probably benign 0.39
R5942:Ccr8 UTSW 9 119,923,772 (GRCm39) missense probably damaging 0.99
R5957:Ccr8 UTSW 9 119,922,893 (GRCm39) missense probably damaging 0.99
R5996:Ccr8 UTSW 9 119,923,529 (GRCm39) missense probably damaging 1.00
R7223:Ccr8 UTSW 9 119,923,683 (GRCm39) missense probably damaging 0.99
R8332:Ccr8 UTSW 9 119,923,440 (GRCm39) missense probably damaging 1.00
R8962:Ccr8 UTSW 9 119,923,613 (GRCm39) missense possibly damaging 0.56
R9344:Ccr8 UTSW 9 119,923,133 (GRCm39) missense probably damaging 1.00
Z1177:Ccr8 UTSW 9 119,923,565 (GRCm39) missense probably benign 0.27
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2020-01-23