|Institutional Source||Beutler Lab|
|Gene Name||guanylate binding protein 2b|
|Synonyms||Gbp1, Mpa1, Mag-1, Gbp-1, Mpa-1|
|Essential gene?||Non essential (E-score: 0.000)|
|Stock #||R8039 (G1)|
|Chromosomal Location||142594847-142619179 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to T at 142618164 bp (GRCm38)|
|Amino Acid Change||Isoleucine to Phenylalanine at position 577 (I577F)|
|Ref Sequence||ENSEMBL: ENSMUSP00000029936 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000029936] [ENSMUST00000165774] [ENSMUST00000169572]|
|AlphaFold||no structure available at present|
AA Change: I577F
PolyPhen 2 Score 0.024 (Sensitivity: 0.95; Specificity: 0.81)
AA Change: I577F
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the guanylate-binding protein (GBP) family. GBPs specifically bind guanine nucleotides (GMP, GDP, and GTP) and contain two of the three consensus motifs found in typical GTP-binding proteins. The encoded protein interacts with a member of the germinal center kinase family. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2017]
PHENOTYPE: Mice homozygous for a targeted allele exhibit increased susceptibility to bacterial infection. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Gbp2b||
(F):5'- CTGGTGTTCAGAACTGTCAGTC -3'
(R):5'- TGAACTGAGTCACCTCATAAGCC -3'
(F):5'- GTTCAGAACTGTCAGTCTACATTTC -3'
(R):5'- TCACATATAAACTCCACTTTGTGAAC -3'