Mutagenetix > Incidental Mutation

Incidental Mutation 'R8040:Fat4'

618458

Institutional Source

Beutler Lab

Gene Symbol

Fat4

Ensembl Gene

ENSMUSG00000046743

Gene Name

FAT atypical cadherin 4

Synonyms

6030410K14Rik

MMRRC Submission

067477-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R8040 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

38886940-39011985 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 38981666 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Histidine at position 3156 (Y3156H)

Ref Sequence

ENSEMBL: ENSMUSP00000061836 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061260]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000061260
AA Change: Y3156H

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000061836
Gene: ENSMUSG00000046743
AA Change: Y3156H

Domain	Start	End	E-Value	Type
low complexity region	2	21	N/A	INTRINSIC
CA	60	133	4.09e-7	SMART
CA	157	248	4.51e-18	SMART
CA	272	351	7.66e-30	SMART
CA	380	473	2.55e-17	SMART
CA	497	580	8.27e-26	SMART
CA	605	687	6.46e-28	SMART
CA	711	791	1e-24	SMART
CA	815	891	3.78e-20	SMART
CA	915	994	8.6e-24	SMART
CA	1018	1098	7.09e-25	SMART
CA	1122	1208	6.78e-22	SMART
CA	1232	1313	2.63e-28	SMART
CA	1337	1418	7.25e-31	SMART
CA	1442	1527	4.58e-19	SMART
CA	1550	1629	4.52e-9	SMART
CA	1651	1738	1.3e-9	SMART
CA	1762	1839	2.01e-24	SMART
CA	1863	1942	3.11e-21	SMART
CA	1966	2049	5.85e-26	SMART
CA	2072	2152	1.88e-29	SMART
CA	2176	2257	3.06e-29	SMART
CA	2282	2362	2.61e-23	SMART
CA	2386	2466	2.99e-32	SMART
CA	2490	2568	9.92e-6	SMART
CA	2588	2669	6.58e-20	SMART
CA	2692	2773	7.25e-31	SMART
CA	2796	2872	1.69e-22	SMART
CA	2896	2983	3.16e-22	SMART
CA	3007	3089	1.01e-15	SMART
CA	3113	3194	1.25e-25	SMART
CA	3218	3298	7e-15	SMART
CA	3322	3405	3.96e-14	SMART
CA	3428	3510	3.41e-27	SMART
CA	3532	3614	5.64e-19	SMART
EGF	3807	3862	1.78e-2	SMART
EGF_CA	3864	3900	2.36e-16	SMART
EGF_CA	3902	3938	7.99e-14	SMART
EGF	3943	3976	1.24e-1	SMART
LamG	3996	4144	4.08e-19	SMART
EGF	4167	4200	5.88e-3	SMART
LamG	4244	4375	1.76e-23	SMART
EGF	4430	4464	1.41e-5	SMART
low complexity region	4514	4526	N/A	INTRINSIC
low complexity region	4533	4550	N/A	INTRINSIC
low complexity region	4840	4849	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

100% (47/47)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the protocadherin family. This gene may play a role in regulating planar cell polarity (PCP). Studies in mice suggest that loss of PCP signaling may cause cystic kidney disease, and mutations in this gene have been associated with Van Maldergem Syndrome 2. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Mar 2014]
PHENOTYPE: Homozygous inactivation of this gene leads to neonatal lethality, reduced birth body size, curly tails, kyphosis, small lungs, renal cysts, and defects in sternum and vertebrae morphology, neural tube width, cochlear elongation, stereocilia orientation, kidney development, and intestinal elongation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb1a	A	T	5: 8,715,035 (GRCm38)	D675V	probably benign	Het
Adam21	T	C	12: 81,560,437 (GRCm38)	R184G	probably benign	Het
Adcy10	A	G	1: 165,552,024 (GRCm38)	K952E	probably damaging	Het
Allc	A	T	12: 28,555,352 (GRCm38)	I316N	probably damaging	Het
Ano8	T	C	8: 71,482,168 (GRCm38)	T432A	probably benign	Het
Cadps2	C	A	6: 23,412,943 (GRCm38)		probably benign	Het
Camsap2	T	C	1: 136,281,247 (GRCm38)	S168G		Het
Ccl19	T	C	4: 42,756,297 (GRCm38)	S12G	probably damaging	Het
Cltc	A	G	11: 86,725,205 (GRCm38)	S412P	probably damaging	Het
Cwc25	A	G	11: 97,750,870 (GRCm38)		probably null	Het
Cxcr4	A	G	1: 128,589,798 (GRCm38)	F42S	probably damaging	Het
Cyp4a12a	A	G	4: 115,326,215 (GRCm38)	Q177R	probably benign	Het
Dnal4	T	C	15: 79,761,968 (GRCm38)	T112A	probably benign	Het
Dock9	C	A	14: 121,651,794 (GRCm38)	G304C	probably benign	Het
Evpl	T	C	11: 116,222,932 (GRCm38)	T1311A	probably damaging	Het
Golgb1	A	G	16: 36,913,479 (GRCm38)	I1070M	possibly damaging	Het
Hcls1	A	G	16: 36,951,149 (GRCm38)	D139G	probably damaging	Het
Ighv1-7	A	G	12: 114,538,770 (GRCm38)	S26P	probably benign	Het
Il1r1	A	T	1: 40,313,349 (GRCm38)	T563S	probably benign	Het
Kcna1	C	T	6: 126,642,740 (GRCm38)	D206N	probably benign	Het
Kcnk4	A	G	19: 6,927,627 (GRCm38)	Y219H	probably damaging	Het
Kcnt2	G	A	1: 140,450,217 (GRCm38)	A403T	probably damaging	Het
Maats1	A	G	16: 38,320,371 (GRCm38)	S407P	possibly damaging	Het
Mdp1	T	A	14: 55,660,066 (GRCm38)	N44I	probably benign	Het
Nceh1	A	G	3: 27,241,225 (GRCm38)	N212D	probably benign	Het
Nynrin	G	A	14: 55,871,525 (GRCm38)	G1363D	probably benign	Het
Olfr1165-ps	T	A	2: 88,101,359 (GRCm38)	R209S	unknown	Het
Olfr26	T	A	9: 38,855,164 (GRCm38)	I34N	probably damaging	Het
Olfr429	T	C	1: 174,089,157 (GRCm38)	I39T	possibly damaging	Het
Olfr986	A	G	9: 40,187,421 (GRCm38)	H102R	probably damaging	Het
Papd7	A	G	13: 69,500,481 (GRCm38)	Y719H	probably damaging	Het
Parp2	T	A	14: 50,810,173 (GRCm38)	M47K	probably benign	Het
Pfdn6	T	C	17: 33,939,977 (GRCm38)		probably benign	Het
Plat	G	T	8: 22,772,232 (GRCm38)	G91W	probably damaging	Het
Plb1	T	A	5: 32,273,069 (GRCm38)	H123Q	possibly damaging	Het
Pml	C	A	9: 58,234,685 (GRCm38)	R288L	probably benign	Het
Prodh2	A	G	7: 30,506,411 (GRCm38)	D238G	probably damaging	Het
Rad51ap2	T	A	12: 11,458,791 (GRCm38)	S905T	probably benign	Het
Sclt1	C	T	3: 41,657,376 (GRCm38)	R487H	probably damaging	Het
Serpina3b	A	G	12: 104,131,076 (GRCm38)	I205M	probably benign	Het
Sowahb	A	G	5: 93,043,433 (GRCm38)	S476P	possibly damaging	Het
Srgap3	C	T	6: 112,739,364 (GRCm38)	R625H	probably benign	Het
Tnxb	T	C	17: 34,716,558 (GRCm38)	Y2611H	probably damaging	Het
Uggt1	T	C	1: 36,211,473 (GRCm38)	E237G	possibly damaging	Het
Vmn1r87	T	A	7: 13,132,159 (GRCm38)	Y67F	possibly damaging	Het
Vmn2r107	G	A	17: 20,375,546 (GRCm38)	C787Y	probably damaging	Het
Zscan4-ps3	T	A	7: 11,612,673 (GRCm38)	I212N	probably damaging	Het

Other mutations in Fat4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00235:Fat4	APN	3	38,982,249 (GRCm38)	missense	probably damaging	1.00
IGL00509:Fat4	APN	3	38,889,039 (GRCm38)	missense	probably damaging	1.00
IGL00698:Fat4	APN	3	38,981,145 (GRCm38)	missense	probably benign	0.17
IGL00934:Fat4	APN	3	38,890,673 (GRCm38)	missense	probably damaging	1.00
IGL01063:Fat4	APN	3	38,890,579 (GRCm38)	missense	possibly damaging	0.80
IGL01123:Fat4	APN	3	38,957,269 (GRCm38)	missense	probably benign	0.00
IGL01313:Fat4	APN	3	39,007,201 (GRCm38)	missense	possibly damaging	0.53
IGL01328:Fat4	APN	3	38,980,658 (GRCm38)	missense	probably damaging	1.00
IGL01328:Fat4	APN	3	38,889,991 (GRCm38)	missense	probably damaging	1.00
IGL01374:Fat4	APN	3	38,887,498 (GRCm38)	missense	probably damaging	1.00
IGL01412:Fat4	APN	3	38,891,181 (GRCm38)	missense	probably benign	0.09
IGL01472:Fat4	APN	3	38,888,070 (GRCm38)	missense	probably damaging	1.00
IGL01514:Fat4	APN	3	38,949,534 (GRCm38)	missense	possibly damaging	0.89
IGL01548:Fat4	APN	3	39,009,257 (GRCm38)	missense	probably damaging	1.00
IGL01548:Fat4	APN	3	38,887,758 (GRCm38)	missense	probably damaging	0.99
IGL01576:Fat4	APN	3	38,888,947 (GRCm38)	missense	probably damaging	1.00
IGL01591:Fat4	APN	3	39,010,375 (GRCm38)	nonsense	probably null
IGL01626:Fat4	APN	3	38,951,032 (GRCm38)	missense	probably damaging	1.00
IGL01746:Fat4	APN	3	38,991,731 (GRCm38)	nonsense	probably null
IGL01800:Fat4	APN	3	38,981,729 (GRCm38)	missense	probably damaging	0.99
IGL01815:Fat4	APN	3	38,888,773 (GRCm38)	missense	probably damaging	1.00
IGL01863:Fat4	APN	3	38,970,619 (GRCm38)	splice site	probably benign
IGL01917:Fat4	APN	3	38,889,730 (GRCm38)	missense	possibly damaging	0.89
IGL01936:Fat4	APN	3	38,979,774 (GRCm38)	missense	probably benign	0.10
IGL02060:Fat4	APN	3	39,010,271 (GRCm38)	missense	probably damaging	1.00
IGL02103:Fat4	APN	3	38,889,199 (GRCm38)	missense	probably damaging	0.97
IGL02119:Fat4	APN	3	38,982,939 (GRCm38)	missense	probably benign	0.10
IGL02124:Fat4	APN	3	38,888,404 (GRCm38)	missense	probably damaging	1.00
IGL02164:Fat4	APN	3	38,996,205 (GRCm38)	critical splice donor site	probably null
IGL02182:Fat4	APN	3	38,890,546 (GRCm38)	missense	probably damaging	1.00
IGL02207:Fat4	APN	3	38,951,263 (GRCm38)	missense	probably benign	0.16
IGL02210:Fat4	APN	3	38,891,853 (GRCm38)	missense	probably benign	0.01
IGL02257:Fat4	APN	3	39,001,139 (GRCm38)	missense	probably benign	0.09
IGL02271:Fat4	APN	3	38,979,919 (GRCm38)	missense	probably benign	0.18
IGL02305:Fat4	APN	3	39,009,988 (GRCm38)	missense	probably damaging	1.00
IGL02314:Fat4	APN	3	38,887,630 (GRCm38)	missense	probably damaging	1.00
IGL02455:Fat4	APN	3	38,951,131 (GRCm38)	missense	possibly damaging	0.48
IGL02468:Fat4	APN	3	38,983,046 (GRCm38)	missense	probably benign
IGL02478:Fat4	APN	3	38,888,215 (GRCm38)	missense	probably damaging	1.00
IGL02480:Fat4	APN	3	39,010,430 (GRCm38)	missense	probably damaging	1.00
IGL02487:Fat4	APN	3	38,887,245 (GRCm38)	missense	probably damaging	1.00
IGL02632:Fat4	APN	3	39,002,764 (GRCm38)	missense	probably benign	0.04
IGL02665:Fat4	APN	3	39,002,836 (GRCm38)	missense	probably benign	0.08
IGL02674:Fat4	APN	3	38,983,337 (GRCm38)	missense	probably benign	0.35
IGL02692:Fat4	APN	3	38,951,086 (GRCm38)	missense	probably damaging	1.00
IGL02710:Fat4	APN	3	38,890,595 (GRCm38)	missense	probably damaging	1.00
IGL02803:Fat4	APN	3	38,889,295 (GRCm38)	missense	probably damaging	1.00
IGL02834:Fat4	APN	3	38,956,744 (GRCm38)	missense	probably damaging	1.00
IGL02891:Fat4	APN	3	38,951,273 (GRCm38)	missense	probably damaging	1.00
IGL02982:Fat4	APN	3	38,890,843 (GRCm38)	missense	probably damaging	1.00
IGL02993:Fat4	APN	3	38,957,155 (GRCm38)	missense	probably damaging	1.00
IGL02996:Fat4	APN	3	38,958,525 (GRCm38)	missense	probably damaging	1.00
IGL03029:Fat4	APN	3	38,982,591 (GRCm38)	missense	possibly damaging	0.46
IGL03124:Fat4	APN	3	38,981,552 (GRCm38)	missense	possibly damaging	0.61
IGL03144:Fat4	APN	3	38,956,859 (GRCm38)	missense	possibly damaging	0.68
IGL03149:Fat4	APN	3	38,991,685 (GRCm38)	missense	probably damaging	1.00
IGL03169:Fat4	APN	3	38,957,398 (GRCm38)	missense	probably benign	0.02
IGL03190:Fat4	APN	3	38,981,241 (GRCm38)	missense	probably damaging	1.00
IGL03272:Fat4	APN	3	39,009,703 (GRCm38)	missense	probably benign
IGL03371:Fat4	APN	3	38,983,187 (GRCm38)	missense	possibly damaging	0.65
IGL03372:Fat4	APN	3	38,889,134 (GRCm38)	missense	possibly damaging	0.88
IGL03388:Fat4	APN	3	38,957,227 (GRCm38)	missense	probably damaging	1.00
IGL03394:Fat4	APN	3	38,892,019 (GRCm38)	missense	probably damaging	0.99
IGL03394:Fat4	APN	3	39,009,364 (GRCm38)	missense	probably damaging	1.00
IGL03405:Fat4	APN	3	38,958,450 (GRCm38)	missense	probably benign	0.02
IGL03410:Fat4	APN	3	38,891,176 (GRCm38)	missense	probably damaging	1.00
Asahi	UTSW	3	38,981,819 (GRCm38)	missense	probably damaging	1.00
Expulsion	UTSW	3	38,889,649 (GRCm38)	missense	probably benign	0.00
heineken	UTSW	3	38,980,380 (GRCm38)	missense	probably damaging	1.00
schlitz	UTSW	3	38,980,659 (GRCm38)	missense	probably damaging	1.00
PIT4696001:Fat4	UTSW	3	38,982,357 (GRCm38)	missense	probably damaging	0.98
PIT4696001:Fat4	UTSW	3	38,889,004 (GRCm38)	missense	probably benign	0.04
R0015:Fat4	UTSW	3	38,982,503 (GRCm38)	missense	probably damaging	1.00
R0015:Fat4	UTSW	3	38,982,503 (GRCm38)	missense	probably damaging	1.00
R0078:Fat4	UTSW	3	38,888,931 (GRCm38)	missense	probably benign	0.35
R0100:Fat4	UTSW	3	38,980,248 (GRCm38)	missense	probably damaging	1.00
R0100:Fat4	UTSW	3	38,980,248 (GRCm38)	missense	probably damaging	1.00
R0201:Fat4	UTSW	3	38,891,596 (GRCm38)	missense	probably damaging	0.99
R0280:Fat4	UTSW	3	38,890,816 (GRCm38)	missense	probably benign
R0357:Fat4	UTSW	3	38,891,227 (GRCm38)	missense	probably damaging	1.00
R0409:Fat4	UTSW	3	38,977,413 (GRCm38)	missense	probably damaging	1.00
R0498:Fat4	UTSW	3	38,980,637 (GRCm38)	missense	probably benign	0.00
R0502:Fat4	UTSW	3	39,002,924 (GRCm38)	missense	probably damaging	0.98
R0506:Fat4	UTSW	3	38,888,314 (GRCm38)	missense	probably benign	0.00
R0532:Fat4	UTSW	3	38,981,721 (GRCm38)	missense	probably benign	0.02
R0616:Fat4	UTSW	3	38,942,870 (GRCm38)	missense	probably damaging	1.00
R0630:Fat4	UTSW	3	39,000,172 (GRCm38)	missense	probably damaging	1.00
R0678:Fat4	UTSW	3	38,889,694 (GRCm38)	missense	probably damaging	1.00
R0685:Fat4	UTSW	3	39,001,178 (GRCm38)	missense	probably benign
R0729:Fat4	UTSW	3	39,000,295 (GRCm38)	splice site	probably benign
R0748:Fat4	UTSW	3	38,887,828 (GRCm38)	missense	possibly damaging	0.67
R0811:Fat4	UTSW	3	38,957,474 (GRCm38)	missense	probably damaging	1.00
R0812:Fat4	UTSW	3	38,957,474 (GRCm38)	missense	probably damaging	1.00
R0830:Fat4	UTSW	3	38,999,109 (GRCm38)	missense	probably benign	0.26
R0841:Fat4	UTSW	3	38,995,998 (GRCm38)	missense	probably damaging	0.99
R0884:Fat4	UTSW	3	38,982,858 (GRCm38)	missense	possibly damaging	0.89
R1056:Fat4	UTSW	3	38,891,392 (GRCm38)	missense	probably damaging	1.00
R1066:Fat4	UTSW	3	38,957,227 (GRCm38)	missense	probably damaging	1.00
R1078:Fat4	UTSW	3	38,983,086 (GRCm38)	missense	probably benign	0.10
R1084:Fat4	UTSW	3	38,979,825 (GRCm38)	missense	possibly damaging	0.88
R1118:Fat4	UTSW	3	38,982,942 (GRCm38)	missense	possibly damaging	0.88
R1213:Fat4	UTSW	3	38,890,371 (GRCm38)	missense	probably benign	0.01
R1418:Fat4	UTSW	3	38,890,813 (GRCm38)	missense	probably damaging	1.00
R1475:Fat4	UTSW	3	38,888,323 (GRCm38)	missense	probably damaging	1.00
R1487:Fat4	UTSW	3	38,995,917 (GRCm38)	missense	possibly damaging	0.77
R1511:Fat4	UTSW	3	38,983,076 (GRCm38)	missense	probably damaging	0.97
R1534:Fat4	UTSW	3	38,890,089 (GRCm38)	missense	probably damaging	1.00
R1558:Fat4	UTSW	3	38,888,986 (GRCm38)	missense	probably damaging	1.00
R1586:Fat4	UTSW	3	38,888,860 (GRCm38)	missense	probably damaging	1.00
R1592:Fat4	UTSW	3	39,007,177 (GRCm38)	missense	probably damaging	0.99
R1655:Fat4	UTSW	3	38,957,318 (GRCm38)	missense	probably damaging	0.97
R1662:Fat4	UTSW	3	38,980,779 (GRCm38)	missense	probably damaging	1.00
R1710:Fat4	UTSW	3	38,951,155 (GRCm38)	missense	probably damaging	1.00
R1731:Fat4	UTSW	3	38,891,310 (GRCm38)	missense	probably damaging	1.00
R1761:Fat4	UTSW	3	38,887,489 (GRCm38)	missense	possibly damaging	0.61
R1770:Fat4	UTSW	3	39,010,268 (GRCm38)	missense	probably damaging	1.00
R1828:Fat4	UTSW	3	38,983,458 (GRCm38)	missense	probably damaging	1.00
R1835:Fat4	UTSW	3	38,983,571 (GRCm38)	missense	probably benign	0.00
R1846:Fat4	UTSW	3	38,982,383 (GRCm38)	missense	probably benign	0.00
R1861:Fat4	UTSW	3	39,010,484 (GRCm38)	missense	probably benign	0.09
R1871:Fat4	UTSW	3	38,981,072 (GRCm38)	missense	possibly damaging	0.63
R1981:Fat4	UTSW	3	38,991,664 (GRCm38)	missense	probably damaging	1.00
R1988:Fat4	UTSW	3	38,996,090 (GRCm38)	missense	probably damaging	1.00
R1988:Fat4	UTSW	3	38,887,115 (GRCm38)	missense	probably benign
R2056:Fat4	UTSW	3	38,891,170 (GRCm38)	missense	possibly damaging	0.88
R2058:Fat4	UTSW	3	38,891,170 (GRCm38)	missense	possibly damaging	0.88
R2059:Fat4	UTSW	3	38,891,170 (GRCm38)	missense	possibly damaging	0.88
R2070:Fat4	UTSW	3	39,010,655 (GRCm38)	missense	probably benign	0.00
R2078:Fat4	UTSW	3	38,889,673 (GRCm38)	missense	probably damaging	1.00
R2114:Fat4	UTSW	3	38,981,484 (GRCm38)	missense	probably benign	0.01
R2135:Fat4	UTSW	3	38,980,733 (GRCm38)	missense	probably damaging	0.98
R2152:Fat4	UTSW	3	38,983,395 (GRCm38)	missense	probably damaging	1.00
R2153:Fat4	UTSW	3	38,983,395 (GRCm38)	missense	probably damaging	1.00
R2154:Fat4	UTSW	3	38,887,539 (GRCm38)	missense	probably damaging	1.00
R2196:Fat4	UTSW	3	38,981,417 (GRCm38)	missense	probably benign	0.23
R2211:Fat4	UTSW	3	38,891,527 (GRCm38)	missense	possibly damaging	0.77
R2219:Fat4	UTSW	3	39,010,215 (GRCm38)	missense	probably damaging	1.00
R2247:Fat4	UTSW	3	38,892,049 (GRCm38)	missense	probably damaging	1.00
R2263:Fat4	UTSW	3	38,888,989 (GRCm38)	missense	possibly damaging	0.93
R2264:Fat4	UTSW	3	38,890,422 (GRCm38)	missense	probably benign	0.25
R2274:Fat4	UTSW	3	38,995,899 (GRCm38)	missense	possibly damaging	0.47
R2337:Fat4	UTSW	3	38,980,011 (GRCm38)	missense	probably damaging	1.00
R2343:Fat4	UTSW	3	38,957,105 (GRCm38)	missense	probably damaging	0.97
R2365:Fat4	UTSW	3	38,980,419 (GRCm38)	missense	probably benign
R2412:Fat4	UTSW	3	38,957,072 (GRCm38)	missense	probably benign	0.05
R2883:Fat4	UTSW	3	38,980,804 (GRCm38)	missense	probably damaging	1.00
R2942:Fat4	UTSW	3	38,982,336 (GRCm38)	missense	probably damaging	1.00
R2989:Fat4	UTSW	3	39,007,153 (GRCm38)	missense	probably benign
R3103:Fat4	UTSW	3	38,891,940 (GRCm38)	missense	probably benign	0.03
R3158:Fat4	UTSW	3	38,890,791 (GRCm38)	missense	possibly damaging	0.87
R3800:Fat4	UTSW	3	38,981,274 (GRCm38)	missense	possibly damaging	0.48
R3808:Fat4	UTSW	3	38,982,438 (GRCm38)	missense	possibly damaging	0.52
R3848:Fat4	UTSW	3	39,007,261 (GRCm38)	missense	probably benign	0.10
R3850:Fat4	UTSW	3	39,007,261 (GRCm38)	missense	probably benign	0.10
R3957:Fat4	UTSW	3	38,982,346 (GRCm38)	missense	probably benign
R4065:Fat4	UTSW	3	39,009,197 (GRCm38)	missense	probably benign	0.13
R4078:Fat4	UTSW	3	38,980,020 (GRCm38)	missense	probably damaging	1.00
R4096:Fat4	UTSW	3	38,887,875 (GRCm38)	missense	possibly damaging	0.46
R4161:Fat4	UTSW	3	38,942,809 (GRCm38)	missense	possibly damaging	0.95
R4273:Fat4	UTSW	3	38,891,627 (GRCm38)	missense	probably damaging	1.00
R4285:Fat4	UTSW	3	38,889,171 (GRCm38)	missense	probably benign	0.00
R4288:Fat4	UTSW	3	38,891,763 (GRCm38)	missense	probably damaging	1.00
R4407:Fat4	UTSW	3	38,958,540 (GRCm38)	missense	probably benign	0.05
R4528:Fat4	UTSW	3	38,891,294 (GRCm38)	missense	probably benign	0.01
R4547:Fat4	UTSW	3	38,951,283 (GRCm38)	missense	probably damaging	1.00
R4681:Fat4	UTSW	3	38,887,342 (GRCm38)	missense	probably damaging	1.00
R4826:Fat4	UTSW	3	38,982,957 (GRCm38)	missense	probably damaging	1.00
R4855:Fat4	UTSW	3	38,888,317 (GRCm38)	missense	probably benign
R4871:Fat4	UTSW	3	38,891,605 (GRCm38)	missense	probably damaging	1.00
R4897:Fat4	UTSW	3	38,980,632 (GRCm38)	missense	probably damaging	1.00
R4928:Fat4	UTSW	3	39,010,465 (GRCm38)	missense	probably damaging	1.00
R4932:Fat4	UTSW	3	39,007,203 (GRCm38)	missense	probably benign	0.00
R4941:Fat4	UTSW	3	38,957,452 (GRCm38)	missense	probably damaging	1.00
R4943:Fat4	UTSW	3	38,980,173 (GRCm38)	missense	probably benign	0.19
R4959:Fat4	UTSW	3	38,983,046 (GRCm38)	missense	probably benign	0.00
R4973:Fat4	UTSW	3	38,983,046 (GRCm38)	missense	probably benign	0.00
R5098:Fat4	UTSW	3	38,888,289 (GRCm38)	missense	probably benign	0.34
R5163:Fat4	UTSW	3	38,980,797 (GRCm38)	missense	probably damaging	1.00
R5213:Fat4	UTSW	3	38,980,191 (GRCm38)	missense	possibly damaging	0.56
R5328:Fat4	UTSW	3	38,956,868 (GRCm38)	missense	probably damaging	1.00
R5337:Fat4	UTSW	3	39,010,378 (GRCm38)	missense	probably benign	0.44
R5337:Fat4	UTSW	3	38,891,627 (GRCm38)	missense	probably damaging	1.00
R5363:Fat4	UTSW	3	38,888,005 (GRCm38)	missense	probably damaging	1.00
R5380:Fat4	UTSW	3	38,888,864 (GRCm38)	missense	probably damaging	1.00
R5384:Fat4	UTSW	3	38,995,946 (GRCm38)	missense	possibly damaging	0.87
R5422:Fat4	UTSW	3	38,887,245 (GRCm38)	missense	possibly damaging	0.92
R5436:Fat4	UTSW	3	38,891,346 (GRCm38)	missense	probably benign	0.00
R5443:Fat4	UTSW	3	39,010,370 (GRCm38)	missense	probably damaging	1.00
R5501:Fat4	UTSW	3	38,887,215 (GRCm38)	missense	probably benign	0.09
R5571:Fat4	UTSW	3	39,010,274 (GRCm38)	missense	probably damaging	1.00
R5625:Fat4	UTSW	3	38,888,934 (GRCm38)	missense	possibly damaging	0.78
R5652:Fat4	UTSW	3	39,002,968 (GRCm38)	missense	probably damaging	0.99
R5725:Fat4	UTSW	3	38,889,625 (GRCm38)	missense	probably damaging	1.00
R5735:Fat4	UTSW	3	38,949,576 (GRCm38)	missense	probably damaging	1.00
R5739:Fat4	UTSW	3	38,983,134 (GRCm38)	missense	probably benign	0.01
R5766:Fat4	UTSW	3	38,889,468 (GRCm38)	missense	probably damaging	1.00
R5780:Fat4	UTSW	3	38,980,955 (GRCm38)	missense	probably damaging	0.96
R5811:Fat4	UTSW	3	38,891,787 (GRCm38)	missense	probably damaging	1.00
R5829:Fat4	UTSW	3	39,007,305 (GRCm38)	missense	probably damaging	1.00
R5879:Fat4	UTSW	3	38,887,336 (GRCm38)	missense	probably benign
R5933:Fat4	UTSW	3	38,951,375 (GRCm38)	critical splice donor site	probably null
R5938:Fat4	UTSW	3	38,951,239 (GRCm38)	missense	probably damaging	1.00
R5940:Fat4	UTSW	3	38,889,649 (GRCm38)	missense	probably benign	0.00
R5945:Fat4	UTSW	3	38,983,206 (GRCm38)	missense	probably benign	0.19
R5963:Fat4	UTSW	3	39,010,547 (GRCm38)	missense	probably damaging	1.00
R6077:Fat4	UTSW	3	39,002,802 (GRCm38)	missense	probably damaging	1.00
R6158:Fat4	UTSW	3	38,983,262 (GRCm38)	missense	possibly damaging	0.95
R6246:Fat4	UTSW	3	38,891,721 (GRCm38)	missense	probably damaging	1.00
R6253:Fat4	UTSW	3	38,951,356 (GRCm38)	missense	probably damaging	0.99
R6259:Fat4	UTSW	3	39,007,246 (GRCm38)	missense	probably benign	0.18
R6295:Fat4	UTSW	3	39,007,080 (GRCm38)	splice site	probably null
R6387:Fat4	UTSW	3	38,983,785 (GRCm38)	missense	probably damaging	1.00
R6390:Fat4	UTSW	3	38,980,380 (GRCm38)	missense	probably damaging	1.00
R6456:Fat4	UTSW	3	38,983,979 (GRCm38)	missense	possibly damaging	0.90
R6493:Fat4	UTSW	3	38,890,887 (GRCm38)	missense	probably damaging	1.00
R6500:Fat4	UTSW	3	38,981,269 (GRCm38)	nonsense	probably null
R6503:Fat4	UTSW	3	38,982,257 (GRCm38)	missense	probably benign	0.00
R6519:Fat4	UTSW	3	39,002,871 (GRCm38)	missense	probably benign
R6566:Fat4	UTSW	3	38,957,126 (GRCm38)	missense	possibly damaging	0.78
R6576:Fat4	UTSW	3	38,979,690 (GRCm38)	missense	probably benign
R6590:Fat4	UTSW	3	38,983,539 (GRCm38)	missense	probably damaging	1.00
R6658:Fat4	UTSW	3	38,942,928 (GRCm38)	missense	probably benign	0.01
R6662:Fat4	UTSW	3	38,956,821 (GRCm38)	missense	possibly damaging	0.95
R6690:Fat4	UTSW	3	38,983,539 (GRCm38)	missense	probably damaging	1.00
R6807:Fat4	UTSW	3	38,982,440 (GRCm38)	missense	probably benign	0.18
R6823:Fat4	UTSW	3	38,983,939 (GRCm38)	missense	probably benign	0.05
R6824:Fat4	UTSW	3	38,957,525 (GRCm38)	missense	probably benign	0.00
R6830:Fat4	UTSW	3	38,981,817 (GRCm38)	missense	probably benign	0.00
R6925:Fat4	UTSW	3	38,996,204 (GRCm38)	critical splice donor site	probably null
R6948:Fat4	UTSW	3	39,009,446 (GRCm38)	missense	probably damaging	1.00
R6970:Fat4	UTSW	3	38,995,971 (GRCm38)	missense	probably damaging	1.00
R6970:Fat4	UTSW	3	38,981,775 (GRCm38)	missense	probably damaging	1.00
R7017:Fat4	UTSW	3	38,891,543 (GRCm38)	missense	probably benign
R7030:Fat4	UTSW	3	38,981,958 (GRCm38)	missense	probably damaging	1.00
R7044:Fat4	UTSW	3	39,010,811 (GRCm38)	missense	probably benign	0.02
R7044:Fat4	UTSW	3	39,010,810 (GRCm38)	missense	probably benign
R7045:Fat4	UTSW	3	38,888,601 (GRCm38)	missense	probably benign	0.01
R7094:Fat4	UTSW	3	38,889,874 (GRCm38)	missense	probably damaging	1.00
R7111:Fat4	UTSW	3	39,010,533 (GRCm38)	missense	probably damaging	1.00
R7130:Fat4	UTSW	3	38,980,787 (GRCm38)	missense	probably damaging	0.99
R7168:Fat4	UTSW	3	38,980,659 (GRCm38)	missense	probably damaging	1.00
R7192:Fat4	UTSW	3	38,980,464 (GRCm38)	missense	probably benign	0.04
R7194:Fat4	UTSW	3	38,983,895 (GRCm38)	missense	probably damaging	1.00
R7194:Fat4	UTSW	3	38,888,884 (GRCm38)	missense	probably damaging	1.00
R7199:Fat4	UTSW	3	38,977,362 (GRCm38)	missense	probably damaging	0.98
R7213:Fat4	UTSW	3	38,999,087 (GRCm38)	missense	possibly damaging	0.63
R7216:Fat4	UTSW	3	38,891,043 (GRCm38)	missense	probably damaging	1.00
R7225:Fat4	UTSW	3	38,980,176 (GRCm38)	missense	possibly damaging	0.50
R7238:Fat4	UTSW	3	38,890,413 (GRCm38)	missense	probably benign	0.31
R7239:Fat4	UTSW	3	38,983,840 (GRCm38)	missense	possibly damaging	0.85
R7283:Fat4	UTSW	3	38,889,693 (GRCm38)	missense	probably damaging	1.00
R7296:Fat4	UTSW	3	38,889,145 (GRCm38)	nonsense	probably null
R7372:Fat4	UTSW	3	38,890,209 (GRCm38)	missense	probably damaging	1.00
R7400:Fat4	UTSW	3	38,887,924 (GRCm38)	missense	probably damaging	1.00
R7419:Fat4	UTSW	3	39,000,236 (GRCm38)	missense	probably damaging	1.00
R7430:Fat4	UTSW	3	39,009,644 (GRCm38)	missense	probably damaging	1.00
R7430:Fat4	UTSW	3	38,887,450 (GRCm38)	missense	probably damaging	0.97
R7431:Fat4	UTSW	3	39,009,157 (GRCm38)	missense	possibly damaging	0.80
R7486:Fat4	UTSW	3	38,957,427 (GRCm38)	nonsense	probably null
R7501:Fat4	UTSW	3	38,958,448 (GRCm38)	nonsense	probably null
R7533:Fat4	UTSW	3	39,007,257 (GRCm38)	missense	probably benign	0.43
R7542:Fat4	UTSW	3	38,981,621 (GRCm38)	missense	possibly damaging	0.64
R7542:Fat4	UTSW	3	38,981,355 (GRCm38)	missense	possibly damaging	0.56
R7548:Fat4	UTSW	3	38,981,114 (GRCm38)	missense	probably benign	0.13
R7567:Fat4	UTSW	3	38,889,336 (GRCm38)	missense	probably damaging	1.00
R7644:Fat4	UTSW	3	39,010,241 (GRCm38)	missense	possibly damaging	0.64
R7660:Fat4	UTSW	3	38,981,160 (GRCm38)	missense	probably benign
R7665:Fat4	UTSW	3	38,889,178 (GRCm38)	missense	probably benign	0.00
R7676:Fat4	UTSW	3	38,891,697 (GRCm38)	missense	probably damaging	0.98
R7832:Fat4	UTSW	3	39,001,204 (GRCm38)	missense	probably benign	0.00
R7848:Fat4	UTSW	3	38,887,851 (GRCm38)	missense	probably benign
R7883:Fat4	UTSW	3	38,981,819 (GRCm38)	missense	probably damaging	1.00
R7892:Fat4	UTSW	3	38,949,439 (GRCm38)	critical splice acceptor site	probably null
R7904:Fat4	UTSW	3	38,887,541 (GRCm38)	missense	probably damaging	1.00
R7952:Fat4	UTSW	3	38,891,721 (GRCm38)	missense	probably damaging	0.98
R8015:Fat4	UTSW	3	38,981,916 (GRCm38)	missense	possibly damaging	0.79
R8142:Fat4	UTSW	3	38,891,203 (GRCm38)	missense	probably damaging	1.00
R8151:Fat4	UTSW	3	38,892,054 (GRCm38)	missense	probably damaging	0.99
R8163:Fat4	UTSW	3	38,979,732 (GRCm38)	missense	possibly damaging	0.88
R8317:Fat4	UTSW	3	38,958,510 (GRCm38)	missense	possibly damaging	0.80
R8413:Fat4	UTSW	3	39,008,979 (GRCm38)	critical splice acceptor site	probably null
R8447:Fat4	UTSW	3	38,979,675 (GRCm38)	missense	possibly damaging	0.88
R8458:Fat4	UTSW	3	38,981,553 (GRCm38)	missense	probably benign	0.25
R8509:Fat4	UTSW	3	38,981,903 (GRCm38)	missense	probably benign
R8543:Fat4	UTSW	3	38,977,494 (GRCm38)	missense	probably damaging	1.00
R8679:Fat4	UTSW	3	39,010,693 (GRCm38)	missense	probably damaging	1.00
R8726:Fat4	UTSW	3	39,010,498 (GRCm38)	missense	probably damaging	1.00
R8743:Fat4	UTSW	3	38,888,443 (GRCm38)	missense	probably benign	0.16
R8751:Fat4	UTSW	3	38,891,853 (GRCm38)	missense	probably benign	0.01
R8779:Fat4	UTSW	3	38,979,749 (GRCm38)	missense	probably damaging	1.00
R8797:Fat4	UTSW	3	38,999,129 (GRCm38)	missense	probably benign	0.01
R8860:Fat4	UTSW	3	38,892,120 (GRCm38)	missense	probably benign	0.26
R8955:Fat4	UTSW	3	38,983,629 (GRCm38)	missense	probably benign	0.01
R9053:Fat4	UTSW	3	38,887,175 (GRCm38)	nonsense	probably null
R9071:Fat4	UTSW	3	38,983,449 (GRCm38)	missense	probably benign	0.29
R9088:Fat4	UTSW	3	39,007,299 (GRCm38)	missense	probably benign	0.02
R9100:Fat4	UTSW	3	39,010,654 (GRCm38)	missense
R9180:Fat4	UTSW	3	38,888,407 (GRCm38)	missense	possibly damaging	0.78
R9184:Fat4	UTSW	3	38,982,443 (GRCm38)	missense	probably damaging	0.99
R9201:Fat4	UTSW	3	38,890,930 (GRCm38)	missense	probably damaging	1.00
R9206:Fat4	UTSW	3	39,009,241 (GRCm38)	missense	probably damaging	0.99
R9268:Fat4	UTSW	3	38,888,247 (GRCm38)	missense	probably damaging	1.00
R9278:Fat4	UTSW	3	38,891,022 (GRCm38)	missense	probably benign	0.44
R9287:Fat4	UTSW	3	38,891,632 (GRCm38)	missense	probably damaging	0.98
R9355:Fat4	UTSW	3	38,981,898 (GRCm38)	missense	probably damaging	1.00
R9437:Fat4	UTSW	3	38,891,268 (GRCm38)	missense	probably benign	0.00
R9455:Fat4	UTSW	3	38,891,263 (GRCm38)	missense
R9456:Fat4	UTSW	3	38,888,422 (GRCm38)	missense	possibly damaging	0.50
R9476:Fat4	UTSW	3	38,983,737 (GRCm38)	missense	probably benign	0.04
R9510:Fat4	UTSW	3	38,983,737 (GRCm38)	missense	probably benign	0.04
R9511:Fat4	UTSW	3	38,980,653 (GRCm38)	missense	probably damaging	0.98
R9540:Fat4	UTSW	3	39,009,197 (GRCm38)	missense	probably benign
R9568:Fat4	UTSW	3	38,892,007 (GRCm38)	missense	probably damaging	1.00
R9646:Fat4	UTSW	3	38,981,664 (GRCm38)	missense	probably damaging	1.00
R9683:Fat4	UTSW	3	38,889,183 (GRCm38)	missense	possibly damaging	0.52
R9711:Fat4	UTSW	3	39,001,225 (GRCm38)	missense	probably benign	0.00
X0017:Fat4	UTSW	3	39,009,106 (GRCm38)	missense	probably benign	0.00
X0019:Fat4	UTSW	3	38,981,040 (GRCm38)	missense	probably damaging	1.00
X0020:Fat4	UTSW	3	39,000,151 (GRCm38)	missense	probably damaging	1.00
X0024:Fat4	UTSW	3	38,942,902 (GRCm38)	missense	probably benign	0.43
X0064:Fat4	UTSW	3	38,970,752 (GRCm38)	missense	probably damaging	1.00
Z1088:Fat4	UTSW	3	38,958,492 (GRCm38)	missense	probably benign	0.00
Z1088:Fat4	UTSW	3	38,887,050 (GRCm38)	missense	possibly damaging	0.88
Z1176:Fat4	UTSW	3	38,983,815 (GRCm38)	missense	probably damaging	1.00
Z1176:Fat4	UTSW	3	38,983,359 (GRCm38)	missense	probably benign	0.00
Z1177:Fat4	UTSW	3	38,890,347 (GRCm38)	missense	probably damaging	1.00
Z1177:Fat4	UTSW	3	38,888,584 (GRCm38)	missense	probably damaging	1.00
Z1177:Fat4	UTSW	3	38,981,838 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TAAGTGCCACCATCCCTGAGAG -3'
(R):5'- ACGCAATCACAGCATTGGC -3'

Sequencing Primer
(F):5'- TCCCTGAGAGCCACAGCATTG -3'
(R):5'- TTAGGTGGATAACAGTGGTCCCAC -3'

Posted On

2020-01-23