Mutagenetix > Incidental Mutation

Incidental Mutation 'R0661:Prdm15'

61860

Institutional Source

Beutler Lab

Gene Symbol

Prdm15

Ensembl Gene

ENSMUSG00000014039

Gene Name

PR domain containing 15

Synonyms

Zfp298, E130018M06Rik

MMRRC Submission

038846-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0661 (G1)

Quality Score

126

Status

Not validated

Chromosome

Chromosomal Location

97791467-97851850 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 97829682 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 190 (V190E)

Ref Sequence

ENSEMBL: ENSMUSP00000113791 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095849] [ENSMUST00000121584] [ENSMUST00000142295]

AlphaFold

E9Q8T2

Predicted Effect

probably benign
Transcript: ENSMUST00000095849
AA Change: V216E

PolyPhen 2 Score 0.342 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000093533
Gene: ENSMUSG00000014039
AA Change: V216E

Domain	Start	End	E-Value	Type
SET	75	191	5.96e-1	SMART
ZnF_C2H2	223	245	3.99e0	SMART
low complexity region	290	303	N/A	INTRINSIC
ZnF_C2H2	402	424	3.89e-3	SMART
ZnF_C2H2	434	457	2.75e-3	SMART
ZnF_C2H2	468	488	1.88e2	SMART
ZnF_C2H2	495	517	5.42e-2	SMART
ZnF_C2H2	522	544	1.36e-2	SMART
ZnF_C2H2	571	593	6.23e-2	SMART
ZnF_C2H2	598	620	2.75e-3	SMART
low complexity region	642	657	N/A	INTRINSIC
ZnF_C2H2	661	684	2.17e-1	SMART
ZnF_C2H2	689	711	3.24e0	SMART
ZnF_C2H2	725	747	1.38e-3	SMART
ZnF_C2H2	753	775	5.67e-5	SMART
ZnF_C2H2	781	803	3.11e-2	SMART
ZnF_C2H2	809	831	8.34e-3	SMART
ZnF_C2H2	837	859	4.79e-3	SMART
ZnF_C2H2	865	888	4.79e-3	SMART
ZnF_C2H2	894	917	5.06e-2	SMART
low complexity region	948	959	N/A	INTRINSIC
low complexity region	1148	1170	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000121584
AA Change: V190E

PolyPhen 2 Score 0.342 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000113791
Gene: ENSMUSG00000014039
AA Change: V190E

Domain	Start	End	E-Value	Type
SET	49	165	5.96e-1	SMART
ZnF_C2H2	197	219	3.99e0	SMART
low complexity region	264	277	N/A	INTRINSIC
ZnF_C2H2	376	398	3.89e-3	SMART
ZnF_C2H2	408	431	2.75e-3	SMART
ZnF_C2H2	442	462	1.88e2	SMART
ZnF_C2H2	469	491	5.42e-2	SMART
ZnF_C2H2	496	518	1.36e-2	SMART
ZnF_C2H2	545	567	6.23e-2	SMART
ZnF_C2H2	572	594	2.75e-3	SMART
low complexity region	616	631	N/A	INTRINSIC
ZnF_C2H2	635	658	2.17e-1	SMART
ZnF_C2H2	663	685	3.24e0	SMART
ZnF_C2H2	699	721	1.38e-3	SMART
ZnF_C2H2	727	749	5.67e-5	SMART
ZnF_C2H2	755	777	3.11e-2	SMART
ZnF_C2H2	783	805	8.34e-3	SMART
ZnF_C2H2	811	833	4.79e-3	SMART
ZnF_C2H2	839	862	4.79e-3	SMART
ZnF_C2H2	868	891	5.06e-2	SMART
low complexity region	922	933	N/A	INTRINSIC
low complexity region	1122	1144	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000128917

Predicted Effect

probably benign
Transcript: ENSMUST00000142295

SMART Domains

Protein: ENSMUSP00000120497
Gene: ENSMUSG00000014039

Domain	Start	End	E-Value	Type
SET	49	165	5.96e-1	SMART
low complexity region	230	243	N/A	INTRINSIC
ZnF_C2H2	342	364	3.89e-3	SMART
ZnF_C2H2	369	392	2.75e-3	SMART
ZnF_C2H2	403	423	1.88e2	SMART

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.5%
20x: 95.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agtr1b	T	A	3: 20,315,999	T148S	possibly damaging	Het
Anks3	A	G	16: 4,948,334	F124L	probably damaging	Het
Ar	T	A	X: 98,150,565	Y262N	probably damaging	Het
Asxl1	T	A	2: 153,400,724	S1065T	possibly damaging	Het
BC051142	A	T	17: 34,459,913	I217F	possibly damaging	Het
Brip1	A	T	11: 86,110,363	I749N	possibly damaging	Het
C1ra	T	A	6: 124,522,377	H507Q	probably benign	Het
Cdk9	G	A	2: 32,709,820	T135I	probably damaging	Het
Col1a1	A	G	11: 94,949,389	T1088A	unknown	Het
Cpne2	T	C	8: 94,556,039	I283T	possibly damaging	Het
Dcaf17	T	C	2: 71,088,435	L451P	probably damaging	Het
Dhx57	C	T	17: 80,268,864	C599Y	probably damaging	Het
Drd1	T	A	13: 54,053,038	N379Y	possibly damaging	Het
Fsip2	A	G	2: 82,986,169	D4082G	possibly damaging	Het
Grin2a	G	T	16: 9,992,472	P21Q	probably damaging	Het
Heyl	G	T	4: 123,246,031	V128F	probably damaging	Het
Hoxd12	A	G	2: 74,675,892	E216G	probably damaging	Het
Inpp4b	C	A	8: 81,741,462	A18E	possibly damaging	Het
Invs	G	A	4: 48,421,861	R831H	probably benign	Het
Lrrk2	T	C	15: 91,787,016	V2000A	probably damaging	Het
Msh3	T	C	13: 92,345,096	N303D	possibly damaging	Het
Olfr1431	T	C	19: 12,209,704	L46P	probably damaging	Het
Olfr1458	G	A	19: 13,103,278	R3C	possibly damaging	Het
Olfr743	A	G	14: 50,534,095	T228A	probably benign	Het
Pcdh18	A	C	3: 49,753,318	S902R	possibly damaging	Het
Ranbp2	T	G	10: 58,478,733	S1758R	probably benign	Het
Rimbp2	A	G	5: 128,786,710	V738A	probably benign	Het
Rtl5	T	C	X: 102,070,450	H138R	possibly damaging	Het
Sec11a	A	G	7: 80,935,039	V50A	probably damaging	Het
Shroom1	T	C	11: 53,466,937	S772P	possibly damaging	Het
Slc26a6	T	C	9: 108,859,113		probably null	Het
Slf1	A	G	13: 77,083,596	W555R	probably benign	Het
Spx	A	G	6: 142,413,839	S5G	possibly damaging	Het
Tcp1	T	C	17: 12,923,313	V398A	probably benign	Het
Tm6sf1	G	A	7: 81,865,345		probably null	Het
Ufsp2	T	A	8: 45,979,233	M1K	probably null	Het
Usf1	G	A	1: 171,417,499	R196Q	probably damaging	Het
Vmn2r75	G	A	7: 86,165,658	A209V	probably benign	Het
Yme1l1	T	A	2: 23,191,042	M442K	probably damaging	Het
Zfand3	A	G	17: 30,135,398	E63G	probably damaging	Het
Zfp740	A	G	15: 102,212,659	T136A	possibly damaging	Het

Other mutations in Prdm15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00973:Prdm15	APN	16	97806167	splice site	probably benign
IGL01325:Prdm15	APN	16	97806517	missense	probably damaging	1.00
IGL02195:Prdm15	APN	16	97835829	missense	probably damaging	1.00
IGL02473:Prdm15	APN	16	97837605	splice site	probably null
IGL02502:Prdm15	APN	16	97839339	missense	probably damaging	1.00
IGL02604:Prdm15	APN	16	97821942	missense	probably benign
R0408:Prdm15	UTSW	16	97835786	missense	possibly damaging	0.92
R0437:Prdm15	UTSW	16	97812559	missense	probably benign	0.00
R0497:Prdm15	UTSW	16	97794334	missense	possibly damaging	0.63
R0590:Prdm15	UTSW	16	97797761	missense	possibly damaging	0.95
R0630:Prdm15	UTSW	16	97837707	missense	probably null	1.00
R0718:Prdm15	UTSW	16	97812633	missense	possibly damaging	0.89
R1144:Prdm15	UTSW	16	97808708	missense	probably damaging	1.00
R1240:Prdm15	UTSW	16	97837600	missense	probably damaging	0.98
R1605:Prdm15	UTSW	16	97839306	missense	probably damaging	1.00
R1908:Prdm15	UTSW	16	97837685	missense	probably benign	0.27
R2081:Prdm15	UTSW	16	97803780	nonsense	probably null
R2208:Prdm15	UTSW	16	97799264	splice site	probably null
R3787:Prdm15	UTSW	16	97797745	missense	probably benign	0.00
R3890:Prdm15	UTSW	16	97799571	missense	probably damaging	1.00
R4326:Prdm15	UTSW	16	97806515	missense	probably damaging	1.00
R4728:Prdm15	UTSW	16	97821786	missense	probably benign	0.04
R4952:Prdm15	UTSW	16	97806077	missense	probably damaging	0.99
R4998:Prdm15	UTSW	16	97794489	missense	probably damaging	0.97
R5225:Prdm15	UTSW	16	97808675	missense	probably damaging	1.00
R5505:Prdm15	UTSW	16	97816983	missense	possibly damaging	0.76
R5628:Prdm15	UTSW	16	97799623	missense	probably damaging	0.98
R5721:Prdm15	UTSW	16	97807096	missense	possibly damaging	0.74
R5873:Prdm15	UTSW	16	97808689	missense	probably damaging	1.00
R5980:Prdm15	UTSW	16	97812570	nonsense	probably null
R6311:Prdm15	UTSW	16	97799055	missense	probably null	0.08
R6540:Prdm15	UTSW	16	97835805	missense	probably benign	0.13
R7053:Prdm15	UTSW	16	97794542	nonsense	probably null
R7241:Prdm15	UTSW	16	97795741	missense	possibly damaging	0.50
R7468:Prdm15	UTSW	16	97835642	nonsense	probably null
R7473:Prdm15	UTSW	16	97821846	missense	possibly damaging	0.68
R7762:Prdm15	UTSW	16	97818273	missense	probably benign	0.00
R7911:Prdm15	UTSW	16	97812592	missense	probably benign	0.35
R8053:Prdm15	UTSW	16	97835607	missense	probably benign	0.17
R8127:Prdm15	UTSW	16	97837710	missense	probably benign	0.24
R8213:Prdm15	UTSW	16	97807060	missense	probably damaging	1.00
R8708:Prdm15	UTSW	16	97816866	missense	unknown
R8768:Prdm15	UTSW	16	97837688	missense	probably benign
R9000:Prdm15	UTSW	16	97794270	missense	probably benign	0.03
R9513:Prdm15	UTSW	16	97806504	missense	probably damaging	1.00
R9583:Prdm15	UTSW	16	97821942	missense	probably benign
RF002:Prdm15	UTSW	16	97799629	missense	probably damaging	1.00
RF021:Prdm15	UTSW	16	97808756	missense	probably damaging	1.00
Z1177:Prdm15	UTSW	16	97816959	missense	possibly damaging	0.54

Predicted Primers

PCR Primer
(F):5'- GCTTATGTCCACCATATCCAGAGCC -3'
(R):5'- GTGCATGAGACTGAGGATAGTGCC -3'

Sequencing Primer
(F):5'- agtgaatagcccacatgcc -3'
(R):5'- AGTGCCTAGAATACAGTTGCTC -3'

Posted On

2013-07-30