Mutagenetix > Incidental Mutation

Incidental Mutation 'R8052:Olfr146'

619122

Institutional Source

Beutler Lab

Gene Symbol

Olfr146

Ensembl Gene

ENSMUSG00000058820

Gene Name

olfactory receptor 146

Synonyms

GA_x6K02T2PVTD-32715386-32714466, MOR171-10, M15

MMRRC Submission

067489-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.066) question?

Stock #

R8052 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

39018073-39023377 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 39019487 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Lysine at position 18 (T18K)

Ref Sequence

ENSEMBL: ENSMUSP00000151064 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073671] [ENSMUST00000214369] [ENSMUST00000214410] [ENSMUST00000215383]

AlphaFold

Q60884

Predicted Effect

probably damaging
Transcript: ENSMUST00000073671
AA Change: T18K

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000073352
Gene: ENSMUSG00000058820
AA Change: T18K

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	306	7.6e-54	PFAM
Pfam:7tm_1	41	290	8.1e-21	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000214369
AA Change: T18K

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

Predicted Effect

probably damaging
Transcript: ENSMUST00000214410
AA Change: T18K

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

Predicted Effect

probably damaging
Transcript: ENSMUST00000215383
AA Change: T18K

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.5%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700123L14Rik	TCC	TCCCCC	6: 96,165,103 (GRCm38)		probably benign	Het
1700123L14Rik	TCC	TCCGCC	6: 96,165,097 (GRCm38)		probably benign	Het
2010111I01Rik	G	A	13: 63,068,251 (GRCm38)	V446I	probably damaging	Het
Abi1	T	C	2: 22,953,543 (GRCm38)	T297A	probably benign	Het
Acadl	G	T	1: 66,853,178 (GRCm38)	T162K	probably benign	Het
Aldoart2	A	T	12: 55,565,751 (GRCm38)	I154F	probably damaging	Het
Alkbh8	G	A	9: 3,385,478 (GRCm38)	R625H	probably damaging	Het
Ankrd31	T	A	13: 96,832,528 (GRCm38)	V891E	probably benign	Het
Atp8b5	C	T	4: 43,356,982 (GRCm38)	R577*	probably null	Het
Capn3	G	T	2: 120,486,386 (GRCm38)	E285D	probably benign	Het
Cd79b	G	T	11: 106,313,700 (GRCm38)	P87T	probably damaging	Het
Celsr2	T	G	3: 108,412,655 (GRCm38)	D947A	probably damaging	Het
Csmd3	A	C	15: 47,706,387 (GRCm38)	S1213R		Het
Cyp2j5	T	C	4: 96,664,004 (GRCm38)	M3V	probably benign	Het
Ddi1	C	T	9: 6,265,787 (GRCm38)	R194K	probably benign	Het
Decr1	T	C	4: 15,933,019 (GRCm38)	K49R	probably benign	Het
Dnah10	A	C	5: 124,828,511 (GRCm38)	E4130A	probably benign	Het
Dst	A	G	1: 34,284,363 (GRCm38)	D4648G	probably damaging	Het
Ecd	A	G	14: 20,329,952 (GRCm38)		probably null	Het
Erbin	A	T	13: 103,834,356 (GRCm38)	Y917*	probably null	Het
Evpl	T	C	11: 116,223,163 (GRCm38)	K1234E	probably benign	Het
F11r	T	A	1: 171,461,623 (GRCm38)	Y218N	possibly damaging	Het
Fam43a	C	G	16: 30,601,804 (GRCm38)	T402S	probably benign	Het
Frem2	T	C	3: 53,549,643 (GRCm38)	N2096S	probably benign	Het
Gm7697	A	T	8: 69,522,752 (GRCm38)	Y21N	possibly damaging	Het
Gpd2	T	A	2: 57,306,950 (GRCm38)	Y172*	probably null	Het
Hscb	A	G	5: 110,835,978 (GRCm38)	V90A	probably benign	Het
Iqgap2	T	C	13: 95,657,879 (GRCm38)	D1195G	probably damaging	Het
Map2k2	T	C	10: 81,115,066 (GRCm38)	I115T	probably damaging	Het
Mast2	C	T	4: 116,312,975 (GRCm38)	R707H	probably damaging	Het
Mindy4	A	G	6: 55,300,992 (GRCm38)	N607S	probably damaging	Het
Mrpl9	T	A	3: 94,443,743 (GRCm38)	Y77N	probably damaging	Het
Muc16	G	A	9: 18,659,051 (GRCm38)	T724I	unknown	Het
Nat3	A	T	8: 67,547,826 (GRCm38)	Y119F	possibly damaging	Het
Nol7	T	C	13: 43,401,514 (GRCm38)	S208P	probably damaging	Het
Notch3	A	T	17: 32,146,571 (GRCm38)	C1056S	probably damaging	Het
Olfr1039	A	T	2: 86,131,377 (GRCm38)	Y95*	probably null	Het
Olfr399	T	A	11: 74,054,475 (GRCm38)	I95F	probably benign	Het
Olfr71	A	T	4: 43,705,884 (GRCm38)	V228E	probably damaging	Het
Osbpl3	A	C	6: 50,346,015 (GRCm38)	L288R	probably damaging	Het
Oscp1	A	C	4: 126,088,323 (GRCm38)	D352A	possibly damaging	Het
Pcdh9	G	A	14: 93,885,786 (GRCm38)	R983C	probably benign	Het
Pcdhgb8	T	C	18: 37,763,502 (GRCm38)	S542P	probably benign	Het
Pi4ka	T	C	16: 17,356,166 (GRCm38)	T490A		Het
Pkd1l1	A	T	11: 8,947,315 (GRCm38)	D531E		Het
Prr3	G	T	17: 35,979,161 (GRCm38)	D26E	possibly damaging	Het
Psmd8	T	C	7: 29,180,576 (GRCm38)	K24E	probably benign	Het
Rasgrp4	G	A	7: 29,149,937 (GRCm38)	C583Y	probably damaging	Het
Rest	A	G	5: 77,268,324 (GRCm38)	I128M	probably benign	Het
Rftn1	A	T	17: 50,086,579 (GRCm38)	F144I	probably damaging	Het
Rusc2	T	C	4: 43,421,851 (GRCm38)	F757S	probably benign	Het
Ryr1	A	G	7: 29,083,385 (GRCm38)	S1942P	probably benign	Het
Sdk2	C	T	11: 113,854,351 (GRCm38)	R706Q	probably damaging	Het
Sergef	G	T	7: 46,614,638 (GRCm38)	T275K	probably damaging	Het
Serpina10	T	C	12: 103,628,310 (GRCm38)	T217A	probably damaging	Het
Shd	G	C	17: 55,976,235 (GRCm38)	S288T	probably damaging	Het
Siglec15	C	A	18: 78,048,588 (GRCm38)	A133S	possibly damaging	Het
Stat4	C	T	1: 52,079,773 (GRCm38)	P325L	probably damaging	Het
Syt8	G	A	7: 142,440,144 (GRCm38)	G344D	probably damaging	Het
Tes	A	G	6: 17,097,292 (GRCm38)	E133G	probably benign	Het
Tmprss2	T	C	16: 97,568,416 (GRCm38)	Y386C	probably damaging	Het
Tnfrsf11b	A	T	15: 54,252,106 (GRCm38)	L365Q	probably damaging	Het
Tns1	T	A	1: 73,953,437 (GRCm38)	D67V	probably damaging	Het
Tns2	T	C	15: 102,112,845 (GRCm38)	S982P	probably damaging	Het
Tpcn2	A	T	7: 145,260,946 (GRCm38)	F473I	probably benign	Het
Tsg101	A	T	7: 46,892,509 (GRCm38)	I232N	probably damaging	Het
Ttc3	T	C	16: 94,467,989 (GRCm38)	S1977P	probably benign	Het
Ttll11	T	A	2: 35,979,515 (GRCm38)	E37V	unknown	Het
Ttn	A	T	2: 76,818,816 (GRCm38)	V12716E	possibly damaging	Het
Vmn2r70	A	C	7: 85,563,715 (GRCm38)	S495A	probably benign	Het
Zfp974	A	G	7: 27,911,272 (GRCm38)	C343R	probably damaging	Het
Zfy1	A	T	Y: 726,004 (GRCm38)	I587N	possibly damaging	Het

Other mutations in Olfr146

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01533:Olfr146	APN	9	39,018,801 (GRCm38)	missense	probably damaging	0.98
IGL01655:Olfr146	APN	9	39,018,918 (GRCm38)	missense	probably benign	0.00
IGL01804:Olfr146	APN	9	39,019,401 (GRCm38)	missense	probably benign	0.13
IGL02098:Olfr146	APN	9	39,018,891 (GRCm38)	missense	probably damaging	1.00
IGL02719:Olfr146	APN	9	39,019,016 (GRCm38)	missense	probably benign	0.11
R0531:Olfr146	UTSW	9	39,019,176 (GRCm38)	missense	probably damaging	0.97
R1270:Olfr146	UTSW	9	39,019,247 (GRCm38)	missense	possibly damaging	0.90
R1511:Olfr146	UTSW	9	39,019,025 (GRCm38)	missense	probably benign	0.03
R1590:Olfr146	UTSW	9	39,018,957 (GRCm38)	missense	probably benign	0.09
R1649:Olfr146	UTSW	9	39,019,480 (GRCm38)	missense	probably benign	0.03
R3419:Olfr146	UTSW	9	39,019,076 (GRCm38)	missense	probably benign	0.03
R4669:Olfr146	UTSW	9	39,019,379 (GRCm38)	missense	probably benign	0.10
R4788:Olfr146	UTSW	9	39,018,921 (GRCm38)	missense	probably benign	0.07
R5184:Olfr146	UTSW	9	39,018,702 (GRCm38)	missense	probably damaging	0.98
R5581:Olfr146	UTSW	9	39,018,702 (GRCm38)	missense	probably damaging	0.98
R6032:Olfr146	UTSW	9	39,018,965 (GRCm38)	missense	probably benign	0.00
R6032:Olfr146	UTSW	9	39,018,965 (GRCm38)	missense	probably benign	0.00
R6319:Olfr146	UTSW	9	39,019,514 (GRCm38)	missense	probably damaging	1.00
R6626:Olfr146	UTSW	9	39,019,106 (GRCm38)	missense	possibly damaging	0.63
R6693:Olfr146	UTSW	9	39,018,801 (GRCm38)	missense	probably damaging	0.98
R7165:Olfr146	UTSW	9	39,023,270 (GRCm38)	start gained	probably benign
R7947:Olfr146	UTSW	9	39,019,451 (GRCm38)	missense	probably damaging	0.99
R7957:Olfr146	UTSW	9	39,019,053 (GRCm38)	missense	probably benign
R8162:Olfr146	UTSW	9	39,018,953 (GRCm38)	missense	probably benign	0.01
R9004:Olfr146	UTSW	9	39,019,284 (GRCm38)	missense	probably benign	0.01
R9083:Olfr146	UTSW	9	39,018,720 (GRCm38)	missense	probably damaging	1.00
R9584:Olfr146	UTSW	9	39,019,166 (GRCm38)	missense	probably damaging	1.00
Z1088:Olfr146	UTSW	9	39,018,789 (GRCm38)	missense	probably damaging	1.00
Z1191:Olfr146	UTSW	9	39,018,933 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGGGTAGGAGATTTCATTCTTCTC -3'
(R):5'- TCTACAAAGTGAAGATGGTCCAG -3'

Sequencing Primer
(F):5'- CACCAGCATTTTTGGGGTAATGAC -3'
(R):5'- TGAAGATGGTCCAGTAAGAAGTAGTC -3'

Posted On

2020-01-23