|Institutional Source||Beutler Lab|
|Gene Name||cleavage and polyadenylation specific factor 7|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R0662 (G1)|
|Chromosomal Location||10525244-10547735 bp(+) (GRCm38)|
|Type of Mutation||start codon destroyed|
|DNA Base Change (assembly)||T to C at 10526008 bp|
|Amino Acid Change||Methionine to Threonine at position 1 (M1T)|
|Ref Sequence||ENSEMBL: ENSMUSP00000038958 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000025570] [ENSMUST00000038379]|
|Predicted Effect||probably benign
|Predicted Effect||probably null
AA Change: M1T
PolyPhen 2 Score 0.771 (Sensitivity: 0.85; Specificity: 0.92)
AA Change: M1T
|Coding Region Coverage||
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Cleavage factor Im (CFIm) is one of six factors necessary for correct cleavage and polyadenylation of pre-mRNAs. CFIm is composed of three different subunits of 25, 59, and 68 kDa, and it functions as a heterotetramer, with a dimer of the 25 kDa subunit binding to two of the 59 or 68 kDa subunits. The protein encoded by this gene represents the 59 kDa subunit, which can interact with the splicing factor U2 snRNP Auxiliary Factor (U2AF) 65 to link the splicing and polyadenylation complexes. [provided by RefSeq, Oct 2016]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Cpsf7||
(F):5'- ATGCTGCGTCTTGGGAGACTAGAG -3'
(R):5'- TTCATGGTACACCATTGGGAGGGG -3'
(F):5'- GAGACTAGAGCTTGTTGACCC -3'
(R):5'- GAATGGTCAGTCGTCTTCAAAGC -3'