Mutagenetix > Incidental Mutation

Incidental Mutation 'R8055:Tns3'

619329

Institutional Source

Beutler Lab

Gene Symbol

Tns3

Ensembl Gene

ENSMUSG00000020422

Gene Name

tensin 3

Synonyms

F830010I22Rik, Tens1, TEM6

MMRRC Submission

067492-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.327) question?

Stock #

R8055 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

8381652-8614681 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 8495343 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 70 (D70E)

Ref Sequence

ENSEMBL: ENSMUSP00000020695 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020695]

AlphaFold

Q5SSZ5

Predicted Effect

probably damaging
Transcript: ENSMUST00000020695
AA Change: D70E

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000020695
Gene: ENSMUSG00000020422
AA Change: D70E

Domain	Start	End	E-Value	Type
SCOP:d1d5ra2	1	171	5e-28	SMART
PTEN_C2	173	300	1.15e-48	SMART
low complexity region	854	864	N/A	INTRINSIC
low complexity region	1102	1126	N/A	INTRINSIC
SH2	1165	1268	1.32e-18	SMART
PTB	1301	1438	3.14e-24	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a null allele exhibit one third postnatal lethality, reduced body weight, growth retardation, smaller digestive tracts with defects in villi and enterocyte differentiation, abnormal lung morphology, and thinner bones with decreased chondrocyte proliferation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adrm1b	C	A	3: 92,336,139 (GRCm39)	G188W	unknown	Het
Ahcyl1	A	T	3: 107,576,047 (GRCm39)	D353E	probably benign	Het
Alk	G	T	17: 72,206,252 (GRCm39)	P1033T	probably benign	Het
Asb15	G	T	6: 24,556,565 (GRCm39)	C20F	probably benign	Het
BC049715	A	G	6: 136,816,913 (GRCm39)	N51S	possibly damaging	Het
Ccser1	G	A	6: 61,290,757 (GRCm39)	V480M	possibly damaging	Het
Cep295	T	C	9: 15,244,905 (GRCm39)	N1184D	probably benign	Het
Cers6	C	T	2: 68,777,625 (GRCm39)	R111W	probably damaging	Het
Clybl	T	A	14: 122,615,273 (GRCm39)	D204E	probably damaging	Het
Cxcl13	T	C	5: 96,107,763 (GRCm39)	V73A	probably benign	Het
Dchs2	A	G	3: 83,037,032 (GRCm39)	N593S	probably benign	Het
Esco2	A	T	14: 66,069,168 (GRCm39)	N47K	probably benign	Het
Fan1	T	A	7: 64,022,234 (GRCm39)	N340Y	probably damaging	Het
Fmo4	T	C	1: 162,636,015 (GRCm39)	T46A	probably benign	Het
Gm15217	T	C	14: 46,616,911 (GRCm39)		probably benign	Het
Gnb5	G	T	9: 75,250,826 (GRCm39)	A317S	probably benign	Het
Klf14	A	G	6: 30,934,722 (GRCm39)	V304A	probably benign	Het
Klrc2	A	T	6: 129,633,424 (GRCm39)	C209*	probably null	Het
Kmt2a	A	T	9: 44,732,378 (GRCm39)	N2646K	unknown	Het
Krt14	C	G	11: 100,095,584 (GRCm39)	V274L	possibly damaging	Het
Myo16	A	T	8: 10,612,186 (GRCm39)	D1277V	unknown	Het
Myo9a	A	T	9: 59,814,743 (GRCm39)	E2226D	probably damaging	Het
Osbpl8	T	C	10: 111,120,255 (GRCm39)	V631A	possibly damaging	Het
Piezo2	G	A	18: 63,175,882 (GRCm39)	S1833L	probably damaging	Het
Pkp4	T	C	2: 59,138,359 (GRCm39)	V203A	probably benign	Het
Primpol	T	C	8: 47,032,197 (GRCm39)	D459G	probably benign	Het
Prkdc	G	T	16: 15,634,749 (GRCm39)	R3631S	probably benign	Het
Rbbp8nl	T	C	2: 179,920,001 (GRCm39)	T558A	probably benign	Het
Rcc2	G	A	4: 140,429,586 (GRCm39)	C40Y	probably benign	Het
Rdx	G	A	9: 51,997,724 (GRCm39)	R566Q	probably damaging	Het
Rhbdf2	A	C	11: 116,498,191 (GRCm39)	S3A	probably benign	Het
Rpap1	A	T	2: 119,595,284 (GRCm39)	I1319N	probably benign	Het
Sbno2	T	A	10: 79,905,265 (GRCm39)	I206F	possibly damaging	Het
Scn1a	A	G	2: 66,149,845 (GRCm39)	V944A	probably damaging	Het
Scube1	C	T	15: 83,543,226 (GRCm39)		probably null	Het
Slc22a20	G	A	19: 6,021,439 (GRCm39)	A521V	probably benign	Het
Slc6a4	C	A	11: 76,901,424 (GRCm39)	T53K	probably benign	Het
Snapc2	G	A	8: 4,304,322 (GRCm39)	R75Q	probably damaging	Het
Sptan1	A	T	2: 29,884,351 (GRCm39)	K662I	probably benign	Het
Sycp3	T	C	10: 88,298,438 (GRCm39)	S55P	probably damaging	Het
Tet2	A	T	3: 133,173,753 (GRCm39)	V1503D	possibly damaging	Het
Tfrc	T	A	16: 32,437,474 (GRCm39)	N277K	probably benign	Het
Tlx3	A	T	11: 33,151,283 (GRCm39)	V291E	probably damaging	Het
Tnk1	A	T	11: 69,747,327 (GRCm39)	H101Q	probably benign	Het
Trip11	C	A	12: 101,803,924 (GRCm39)	G1938C	probably damaging	Het
Tubg1	T	C	11: 101,014,828 (GRCm39)	L190P	probably damaging	Het
Vmn2r79	A	T	7: 86,686,541 (GRCm39)	S641C	possibly damaging	Het
Wnt8b	A	G	19: 44,481,952 (GRCm39)		probably benign	Het

Other mutations in Tns3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00163:Tns3	APN	11	8,401,066 (GRCm39)	missense	probably benign	0.42
IGL00822:Tns3	APN	11	8,393,976 (GRCm39)	missense	probably damaging	0.99
IGL01075:Tns3	APN	11	8,428,399 (GRCm39)	missense	probably benign	0.45
IGL01286:Tns3	APN	11	8,442,617 (GRCm39)	missense	probably benign	0.01
IGL01680:Tns3	APN	11	8,498,937 (GRCm39)	missense	probably damaging	1.00
IGL01687:Tns3	APN	11	8,442,798 (GRCm39)	missense	probably damaging	1.00
IGL01734:Tns3	APN	11	8,469,192 (GRCm39)	splice site	probably benign
IGL01844:Tns3	APN	11	8,387,177 (GRCm39)	missense	possibly damaging	0.58
IGL01984:Tns3	APN	11	8,498,992 (GRCm39)	nonsense	probably null
IGL02137:Tns3	APN	11	8,442,578 (GRCm39)	missense	possibly damaging	0.93
IGL02273:Tns3	APN	11	8,384,531 (GRCm39)	missense	probably damaging	1.00
IGL02623:Tns3	APN	11	8,387,141 (GRCm39)	missense	probably damaging	1.00
IGL02697:Tns3	APN	11	8,442,346 (GRCm39)	missense	probably benign	0.00
IGL02829:Tns3	APN	11	8,469,564 (GRCm39)	missense	probably damaging	1.00
ANU74:Tns3	UTSW	11	8,442,149 (GRCm39)	missense	probably benign	0.38
R0020:Tns3	UTSW	11	8,495,227 (GRCm39)	critical splice donor site	probably null
R0064:Tns3	UTSW	11	8,385,856 (GRCm39)	nonsense	probably null
R0064:Tns3	UTSW	11	8,385,856 (GRCm39)	nonsense	probably null
R0370:Tns3	UTSW	11	8,395,730 (GRCm39)	missense	possibly damaging	0.80
R0388:Tns3	UTSW	11	8,395,703 (GRCm39)	missense	probably benign	0.07
R0410:Tns3	UTSW	11	8,385,852 (GRCm39)	missense	probably benign	0.02
R0496:Tns3	UTSW	11	8,497,262 (GRCm39)	splice site	probably benign
R0562:Tns3	UTSW	11	8,443,262 (GRCm39)	missense	possibly damaging	0.93
R0626:Tns3	UTSW	11	8,443,121 (GRCm39)	missense	probably benign	0.04
R0736:Tns3	UTSW	11	8,469,474 (GRCm39)	missense	possibly damaging	0.94
R0893:Tns3	UTSW	11	8,443,302 (GRCm39)	missense	probably damaging	1.00
R1367:Tns3	UTSW	11	8,398,704 (GRCm39)	missense	probably benign	0.01
R1386:Tns3	UTSW	11	8,468,261 (GRCm39)	missense	probably benign	0.02
R1975:Tns3	UTSW	11	8,385,738 (GRCm39)	missense	probably benign	0.04
R2205:Tns3	UTSW	11	8,481,719 (GRCm39)	missense	probably damaging	1.00
R2319:Tns3	UTSW	11	8,491,200 (GRCm39)	missense	probably damaging	1.00
R2830:Tns3	UTSW	11	8,385,870 (GRCm39)	missense	probably damaging	1.00
R3720:Tns3	UTSW	11	8,442,999 (GRCm39)	missense	probably damaging	1.00
R3765:Tns3	UTSW	11	8,401,133 (GRCm39)	missense	probably benign	0.00
R3817:Tns3	UTSW	11	8,384,619 (GRCm39)	missense	probably damaging	1.00
R4058:Tns3	UTSW	11	8,442,275 (GRCm39)	missense	probably damaging	1.00
R4599:Tns3	UTSW	11	8,481,747 (GRCm39)	missense	probably damaging	1.00
R4631:Tns3	UTSW	11	8,401,119 (GRCm39)	missense	probably benign	0.30
R4731:Tns3	UTSW	11	8,400,986 (GRCm39)	missense	probably benign	0.28
R4732:Tns3	UTSW	11	8,400,986 (GRCm39)	missense	probably benign	0.28
R4733:Tns3	UTSW	11	8,400,986 (GRCm39)	missense	probably benign	0.28
R5472:Tns3	UTSW	11	8,401,092 (GRCm39)	missense	probably benign
R5749:Tns3	UTSW	11	8,401,177 (GRCm39)	missense	probably benign	0.01
R5807:Tns3	UTSW	11	8,443,211 (GRCm39)	missense	probably damaging	1.00
R5844:Tns3	UTSW	11	8,384,580 (GRCm39)	missense	probably damaging	1.00
R5942:Tns3	UTSW	11	8,385,860 (GRCm39)	missense	probably damaging	1.00
R5982:Tns3	UTSW	11	8,442,245 (GRCm39)	missense	probably damaging	0.99
R6025:Tns3	UTSW	11	8,442,578 (GRCm39)	missense	possibly damaging	0.93
R6266:Tns3	UTSW	11	8,442,987 (GRCm39)	missense	probably damaging	1.00
R6322:Tns3	UTSW	11	8,442,147 (GRCm39)	missense	probably benign	0.01
R6536:Tns3	UTSW	11	8,384,531 (GRCm39)	missense	probably damaging	1.00
R6577:Tns3	UTSW	11	8,499,058 (GRCm39)	missense	probably damaging	1.00
R6577:Tns3	UTSW	11	8,499,057 (GRCm39)	missense	probably damaging	1.00
R6864:Tns3	UTSW	11	8,443,196 (GRCm39)	missense	probably damaging	1.00
R6897:Tns3	UTSW	11	8,481,743 (GRCm39)	missense	probably damaging	1.00
R7108:Tns3	UTSW	11	8,387,251 (GRCm39)	missense	probably benign	0.00
R7443:Tns3	UTSW	11	8,401,442 (GRCm39)	missense	probably benign	0.01
R7459:Tns3	UTSW	11	8,442,793 (GRCm39)	missense	probably benign	0.16
R7474:Tns3	UTSW	11	8,480,894 (GRCm39)	missense	probably damaging	1.00
R7576:Tns3	UTSW	11	8,491,192 (GRCm39)	missense	possibly damaging	0.78
R7979:Tns3	UTSW	11	8,442,701 (GRCm39)	missense	probably benign	0.01
R8057:Tns3	UTSW	11	8,442,773 (GRCm39)	missense	probably benign
R8077:Tns3	UTSW	11	8,395,667 (GRCm39)	missense	probably damaging	1.00
R8518:Tns3	UTSW	11	8,442,971 (GRCm39)	missense	probably damaging	0.96
R8523:Tns3	UTSW	11	8,398,779 (GRCm39)	missense	probably damaging	1.00
R8790:Tns3	UTSW	11	8,468,273 (GRCm39)	missense	probably damaging	0.99
R9228:Tns3	UTSW	11	8,400,094 (GRCm39)	missense	probably damaging	1.00
R9374:Tns3	UTSW	11	8,442,606 (GRCm39)	missense	probably damaging	1.00
R9476:Tns3	UTSW	11	8,395,702 (GRCm39)	missense	probably damaging	0.99
R9510:Tns3	UTSW	11	8,395,702 (GRCm39)	missense	probably damaging	0.99
R9594:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9595:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9596:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9624:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9629:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
T0975:Tns3	UTSW	11	8,401,146 (GRCm39)	missense	probably benign	0.00
T0975:Tns3	UTSW	11	8,499,100 (GRCm39)	start gained	probably benign
T0975:Tns3	UTSW	11	8,429,518 (GRCm39)	missense	probably benign
X0005:Tns3	UTSW	11	8,429,518 (GRCm39)	missense	probably benign
X0005:Tns3	UTSW	11	8,401,224 (GRCm39)	missense	probably benign	0.00
Z1177:Tns3	UTSW	11	8,401,014 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GGAAAATCAGTTGGTGCCTG -3'
(R):5'- GGTAGCCTGCATTTTCGTTC -3'

Sequencing Primer
(F):5'- AGCCTGATCTAATCCACGTGG -3'
(R):5'- AGCCTGCATTTTCGTTCCTATAAAGG -3'

Posted On

2020-01-23