Mutagenetix > Incidental Mutation

Incidental Mutation 'R8056:Brca2'

619372

Institutional Source

Beutler Lab

Gene Symbol

Brca2

Ensembl Gene

ENSMUSG00000041147

Gene Name

breast cancer 2, early onset

Synonyms

Fancd1, RAB163

MMRRC Submission

067493-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R8056 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

150522630-150570329 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 150569306 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 3262 (V3262A)

Ref Sequence

ENSEMBL: ENSMUSP00000144150 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000016279] [ENSMUST00000044620] [ENSMUST00000202031] [ENSMUST00000202279] [ENSMUST00000202291] [ENSMUST00000202313]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000016279

SMART Domains

Protein: ENSMUSP00000016279
Gene: ENSMUSG00000041132

Domain	Start	End	E-Value	Type
low complexity region	15	35	N/A	INTRINSIC
Pfam:AAA_33	42	176	6.1e-19	PFAM
Pfam:AAA_17	42	198	3.2e-8	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000044620
AA Change: V3262A

PolyPhen 2 Score 0.845 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000038576
Gene: ENSMUSG00000041147
AA Change: V3262A

Domain	Start	End	E-Value	Type
low complexity region	36	51	N/A	INTRINSIC
low complexity region	100	123	N/A	INTRINSIC
low complexity region	187	199	N/A	INTRINSIC
low complexity region	746	761	N/A	INTRINSIC
low complexity region	904	917	N/A	INTRINSIC
Pfam:BRCA2	982	1014	2.6e-13	PFAM
Pfam:BRCA2	1193	1225	3.9e-16	PFAM
low complexity region	1239	1252	N/A	INTRINSIC
Pfam:BRCA2	1395	1425	1.4e-13	PFAM
Pfam:BRCA2	1492	1524	1.8e-13	PFAM
Pfam:BRCA2	1624	1655	8.4e-12	PFAM
Pfam:BRCA2	1925	1957	8e-15	PFAM
Pfam:BRCA2	2005	2037	1.7e-11	PFAM
Pfam:BRCA-2_helical	2402	2588	1.3e-94	PFAM
Pfam:BRCA-2_OB1	2591	2717	5.3e-44	PFAM
Tower	2752	2793	2.37e-18	SMART
Pfam:BRCA-2_OB3	2971	3104	1.5e-49	PFAM
low complexity region	3197	3208	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000202031

SMART Domains

Protein: ENSMUSP00000144164
Gene: ENSMUSG00000041132

Domain	Start	End	E-Value	Type
SCOP:d1ly1a_	8	71	3e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000202279

SMART Domains

Protein: ENSMUSP00000143792
Gene: ENSMUSG00000041132

Domain	Start	End	E-Value	Type
low complexity region	15	35	N/A	INTRINSIC
SCOP:d1l4ua_	42	58	1e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000202291

Predicted Effect

possibly damaging
Transcript: ENSMUST00000202313
AA Change: V3262A

PolyPhen 2 Score 0.845 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000144150
Gene: ENSMUSG00000041147
AA Change: V3262A

Domain	Start	End	E-Value	Type
low complexity region	36	51	N/A	INTRINSIC
low complexity region	100	123	N/A	INTRINSIC
low complexity region	187	199	N/A	INTRINSIC
low complexity region	746	761	N/A	INTRINSIC
low complexity region	904	917	N/A	INTRINSIC
Pfam:BRCA2	982	1014	2.6e-13	PFAM
Pfam:BRCA2	1193	1225	3.9e-16	PFAM
low complexity region	1239	1252	N/A	INTRINSIC
Pfam:BRCA2	1395	1425	1.4e-13	PFAM
Pfam:BRCA2	1492	1524	1.8e-13	PFAM
Pfam:BRCA2	1624	1655	8.4e-12	PFAM
Pfam:BRCA2	1925	1957	8e-15	PFAM
Pfam:BRCA2	2005	2037	1.7e-11	PFAM
Pfam:BRCA-2_helical	2402	2588	1.3e-94	PFAM
Pfam:BRCA-2_OB1	2591	2717	5.3e-44	PFAM
Tower	2752	2793	2.37e-18	SMART
Pfam:BRCA-2_OB3	2971	3104	1.5e-49	PFAM
low complexity region	3197	3208	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (75/75)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq, Dec 2008]
PHENOTYPE: Homozygous null mutants are embryonic lethal with abnormalities including growth retardation, neural tube defects, and mesoderm abnormalities; conditional mutations cause genetic instability and enhanced tumor formation; mutants with truncated BRCA2 protein survive, are small, infertile, show improper tissue differentiation and develop lymphomas and carcinomas. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1300017J02Rik	A	G	9: 103,266,224 (GRCm38)	C438R	probably damaging	Het
2900011O08Rik	T	A	16: 14,038,316 (GRCm38)		probably benign	Het
9930111J21Rik2	T	C	11: 49,020,082 (GRCm38)	E508G	probably benign	Het
Acsl3	C	T	1: 78,681,894 (GRCm38)	L88F	probably damaging	Het
Adamtsl1	C	T	4: 86,342,032 (GRCm38)	P835S	possibly damaging	Het
Agrn	A	G	4: 156,170,411 (GRCm38)	V1616A	probably benign	Het
Bsn	T	C	9: 108,105,307 (GRCm38)	D977G		Het
Cic	G	T	7: 25,290,941 (GRCm38)	A1956S	possibly damaging	Het
Clip4	C	A	17: 71,803,592 (GRCm38)	T189K	probably damaging	Het
Col12a1	T	C	9: 79,599,938 (GRCm38)	E279G		Het
Col24a1	A	C	3: 145,314,164 (GRCm38)	I99L	possibly damaging	Het
Cpne3	A	G	4: 19,532,426 (GRCm38)	V329A	possibly damaging	Het
Cyp17a1	T	C	19: 46,670,591 (GRCm38)	I204V	possibly damaging	Het
Cyp2c66	A	G	19: 39,142,041 (GRCm38)	I107V	probably benign	Het
Dennd4c	A	G	4: 86,844,976 (GRCm38)	R1840G	probably null	Het
Dhx36	A	T	3: 62,488,591 (GRCm38)	L465Q	possibly damaging	Het
Edc4	A	G	8: 105,890,484 (GRCm38)		probably benign	Het
Fam133b	A	T	5: 3,565,744 (GRCm38)	R215S	unknown	Het
Fbxo18	G	A	2: 11,743,630 (GRCm38)	T985I	probably benign	Het
Fras1	A	G	5: 96,744,774 (GRCm38)	D2911G	probably damaging	Het
Gm11639	A	G	11: 104,909,070 (GRCm38)	T2931A	probably damaging	Het
Gm13889	A	T	2: 93,956,675 (GRCm38)	D151E	probably damaging	Het
Gosr2	C	A	11: 103,697,704 (GRCm38)		probably benign	Het
Igkv8-26	T	A	6: 70,193,722 (GRCm38)	L99H	probably damaging	Het
Kif16b	A	G	2: 142,712,842 (GRCm38)	F679L	probably damaging	Het
Kif1a	G	C	1: 93,054,701 (GRCm38)		probably benign	Het
Kif3b	G	C	2: 153,330,059 (GRCm38)	R716S	possibly damaging	Het
Lilra6	A	T	7: 3,912,552 (GRCm38)	C395S	probably damaging	Het
Lrp5	A	G	19: 3,597,337 (GRCm38)	F1302L	probably damaging	Het
Map2	T	G	1: 66,415,620 (GRCm38)	V1223G	probably damaging	Het
Mical1	T	A	10: 41,481,172 (GRCm38)	N324K	probably damaging	Het
Morc3	T	A	16: 93,845,176 (GRCm38)	H94Q	probably benign	Het
Mpped1	T	C	15: 83,836,462 (GRCm38)	V199A	possibly damaging	Het
Msx1	G	A	5: 37,824,200 (GRCm38)	T45I	probably benign	Het
Myh7	G	A	14: 54,973,319 (GRCm38)	Q1714*	probably null	Het
Narf	T	C	11: 121,245,344 (GRCm38)	V182A	possibly damaging	Het
Ncapd2	G	A	6: 125,171,043 (GRCm38)	T1107M	probably damaging	Het
Nek3	A	G	8: 22,129,343 (GRCm38)		probably null	Het
Neurog1	G	T	13: 56,251,410 (GRCm38)	P175T	probably damaging	Het
Nlrp9c	T	C	7: 26,385,687 (GRCm38)	T156A	probably damaging	Het
Olfr1377	T	A	11: 50,985,541 (GRCm38)	V280E	probably damaging	Het
Olfr434	A	T	6: 43,217,044 (GRCm38)	I44F	probably damaging	Het
Olfr788	G	A	10: 129,473,192 (GRCm38)	A167T	probably benign	Het
Orm3	A	G	4: 63,359,357 (GRCm38)	E194G	probably benign	Het
Pga5	A	C	19: 10,676,797 (GRCm38)		probably benign	Het
Pik3r2	G	T	8: 70,772,367 (GRCm38)	P151T	probably benign	Het
Pkp2	T	C	16: 16,213,400 (GRCm38)	C10R	probably benign	Het
Plxdc1	T	A	11: 97,978,517 (GRCm38)	R82W	probably damaging	Het
Polr2c	G	A	8: 94,860,267 (GRCm38)	A54T	probably benign	Het
Rab31	T	C	17: 65,717,508 (GRCm38)	I59V	probably benign	Het
Rasgrf2	T	C	13: 92,030,813 (GRCm38)	M251V	probably damaging	Het
Rcc2	G	A	4: 140,702,275 (GRCm38)	C40Y	probably benign	Het
Rfc1	A	C	5: 65,294,093 (GRCm38)		probably benign	Het
Rhag	T	C	17: 40,828,788 (GRCm38)	I137T	probably damaging	Het
Rnf216	T	C	5: 142,992,861 (GRCm38)	M841V	probably benign	Het
Scaf11	C	A	15: 96,414,817 (GRCm38)	E1448*	probably null	Het
Sept11	T	C	5: 93,167,576 (GRCm38)	L388P	unknown	Het
Serpina1b	A	T	12: 103,817,878 (GRCm38)		probably benign	Het
Skint10	A	G	4: 112,715,813 (GRCm38)	I262T	probably benign	Het
Slc13a4	C	T	6: 35,268,952 (GRCm38)	G586E	probably damaging	Het
Slc8a1	C	T	17: 81,647,923 (GRCm38)	G562E	probably damaging	Het
Slc8b1	T	A	5: 120,520,617 (GRCm38)	L126Q	probably damaging	Het
Smg1	A	G	7: 118,160,366 (GRCm38)	Y2086H	unknown	Het
Taf1c	A	T	8: 119,603,463 (GRCm38)	D47E	probably benign	Het
Tbx5	A	C	5: 119,853,613 (GRCm38)	M250L	probably benign	Het
Tekt3	T	C	11: 63,083,959 (GRCm38)		probably null	Het
Tex43	A	G	18: 56,594,691 (GRCm38)	N79S		Het
Topors	T	C	4: 40,262,221 (GRCm38)	I354M	probably benign	Het
Ttn	C	A	2: 76,948,230 (GRCm38)	G1309V	unknown	Het
Ttyh1	C	T	7: 4,124,623 (GRCm38)		probably benign	Het
Ubac1	A	G	2: 26,007,897 (GRCm38)	I237T	probably benign	Het
Vmn1r197	T	A	13: 22,328,218 (GRCm38)	V103D	probably damaging	Het
Zfp184	T	C	13: 21,958,838 (GRCm38)	F238S	probably damaging	Het
Zfp445	A	G	9: 122,851,967 (GRCm38)	F970L	possibly damaging	Het
Zfp90	A	T	8: 106,424,480 (GRCm38)	E275V	probably damaging	Het
Zscan12	C	G	13: 21,369,322 (GRCm38)	Q439E	probably benign	Het

Other mutations in Brca2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00332:Brca2	APN	5	150,539,898 (GRCm38)	missense	probably benign	0.18
IGL00392:Brca2	APN	5	150,541,240 (GRCm38)	missense	probably benign	0.02
IGL00557:Brca2	APN	5	150,560,538 (GRCm38)	missense	probably benign
IGL00798:Brca2	APN	5	150,539,463 (GRCm38)	missense	probably benign	0.30
IGL00933:Brca2	APN	5	150,542,404 (GRCm38)	missense	probably benign	0.04
IGL00964:Brca2	APN	5	150,532,310 (GRCm38)	missense	probably damaging	1.00
IGL01152:Brca2	APN	5	150,542,390 (GRCm38)	missense	probably damaging	0.99
IGL01577:Brca2	APN	5	150,541,620 (GRCm38)	nonsense	probably null
IGL01585:Brca2	APN	5	150,539,516 (GRCm38)	missense	possibly damaging	0.76
IGL01732:Brca2	APN	5	150,542,387 (GRCm38)	missense	probably benign	0.13
IGL01809:Brca2	APN	5	150,531,061 (GRCm38)	splice site	probably null
IGL01911:Brca2	APN	5	150,567,613 (GRCm38)	missense	probably damaging	0.96
IGL02113:Brca2	APN	5	150,540,979 (GRCm38)	missense	possibly damaging	0.95
IGL02313:Brca2	APN	5	150,538,661 (GRCm38)	missense	probably damaging	1.00
IGL02342:Brca2	APN	5	150,542,824 (GRCm38)	missense	possibly damaging	0.94
IGL02508:Brca2	APN	5	150,543,308 (GRCm38)	missense	possibly damaging	0.85
IGL02532:Brca2	APN	5	150,550,862 (GRCm38)	missense	probably damaging	1.00
IGL02646:Brca2	APN	5	150,560,790 (GRCm38)	missense	possibly damaging	0.89
IGL02738:Brca2	APN	5	150,567,035 (GRCm38)	missense	probably damaging	1.00
IGL02833:Brca2	APN	5	150,541,790 (GRCm38)	missense	possibly damaging	0.83
IGL02871:Brca2	APN	5	150,542,552 (GRCm38)	missense	probably benign	0.13
IGL02995:Brca2	APN	5	150,529,488 (GRCm38)	missense	probably damaging	1.00
IGL03105:Brca2	APN	5	150,560,485 (GRCm38)	missense	probably benign	0.02
BB007:Brca2	UTSW	5	150,558,510 (GRCm38)	missense	probably damaging	0.96
BB017:Brca2	UTSW	5	150,558,510 (GRCm38)	missense	probably damaging	0.96
R0219:Brca2	UTSW	5	150,523,175 (GRCm38)	splice site	probably benign
R0416:Brca2	UTSW	5	150,569,392 (GRCm38)	missense	possibly damaging	0.93
R0441:Brca2	UTSW	5	150,541,857 (GRCm38)	missense	probably damaging	0.96
R0548:Brca2	UTSW	5	150,544,935 (GRCm38)	missense	probably damaging	0.96
R0745:Brca2	UTSW	5	150,544,882 (GRCm38)	splice site	probably benign
R0799:Brca2	UTSW	5	150,560,193 (GRCm38)	missense	probably damaging	0.99
R1165:Brca2	UTSW	5	150,542,747 (GRCm38)	missense	probably damaging	0.98
R1247:Brca2	UTSW	5	150,541,274 (GRCm38)	missense	probably damaging	1.00
R1403:Brca2	UTSW	5	150,542,649 (GRCm38)	missense	probably benign	0.22
R1403:Brca2	UTSW	5	150,542,649 (GRCm38)	missense	probably benign	0.22
R1444:Brca2	UTSW	5	150,542,450 (GRCm38)	missense	probably benign
R1466:Brca2	UTSW	5	150,552,258 (GRCm38)	missense	probably damaging	0.99
R1466:Brca2	UTSW	5	150,552,258 (GRCm38)	missense	probably damaging	0.99
R1584:Brca2	UTSW	5	150,552,258 (GRCm38)	missense	probably damaging	0.99
R1599:Brca2	UTSW	5	150,548,713 (GRCm38)	nonsense	probably null
R1600:Brca2	UTSW	5	150,560,830 (GRCm38)	splice site	probably benign
R1822:Brca2	UTSW	5	150,540,198 (GRCm38)	missense	probably benign	0.06
R1824:Brca2	UTSW	5	150,536,922 (GRCm38)	missense	possibly damaging	0.94
R2037:Brca2	UTSW	5	150,540,669 (GRCm38)	missense	probably benign
R2131:Brca2	UTSW	5	150,557,129 (GRCm38)	missense	probably damaging	1.00
R2203:Brca2	UTSW	5	150,539,502 (GRCm38)	missense	possibly damaging	0.58
R2208:Brca2	UTSW	5	150,532,344 (GRCm38)	missense	probably damaging	0.96
R2293:Brca2	UTSW	5	150,560,534 (GRCm38)	missense	possibly damaging	0.86
R2517:Brca2	UTSW	5	150,539,672 (GRCm38)	missense	probably benign	0.04
R2566:Brca2	UTSW	5	150,541,762 (GRCm38)	missense	probably benign	0.03
R3422:Brca2	UTSW	5	150,543,121 (GRCm38)	missense	possibly damaging	0.91
R3917:Brca2	UTSW	5	150,540,827 (GRCm38)	missense	probably damaging	0.96
R3946:Brca2	UTSW	5	150,536,704 (GRCm38)	missense	probably damaging	0.96
R4176:Brca2	UTSW	5	150,539,633 (GRCm38)	nonsense	probably null
R4255:Brca2	UTSW	5	150,541,169 (GRCm38)	missense	possibly damaging	0.92
R4450:Brca2	UTSW	5	150,536,053 (GRCm38)	missense	probably damaging	0.96
R4603:Brca2	UTSW	5	150,536,165 (GRCm38)	missense	possibly damaging	0.86
R4681:Brca2	UTSW	5	150,552,398 (GRCm38)	splice site	probably null
R4755:Brca2	UTSW	5	150,559,987 (GRCm38)	splice site	probably null
R4762:Brca2	UTSW	5	150,531,116 (GRCm38)	missense	probably benign	0.00
R4824:Brca2	UTSW	5	150,539,735 (GRCm38)	missense	probably damaging	1.00
R4887:Brca2	UTSW	5	150,556,937 (GRCm38)	missense	probably damaging	1.00
R5020:Brca2	UTSW	5	150,560,436 (GRCm38)	missense	probably damaging	1.00
R5159:Brca2	UTSW	5	150,542,108 (GRCm38)	missense	possibly damaging	0.93
R5216:Brca2	UTSW	5	150,542,980 (GRCm38)	missense	probably damaging	0.99
R5269:Brca2	UTSW	5	150,539,223 (GRCm38)	missense	possibly damaging	0.75
R5274:Brca2	UTSW	5	150,539,689 (GRCm38)	missense	probably benign	0.00
R5589:Brca2	UTSW	5	150,557,132 (GRCm38)	missense	possibly damaging	0.67
R5619:Brca2	UTSW	5	150,557,114 (GRCm38)	missense	probably damaging	0.96
R5641:Brca2	UTSW	5	150,556,899 (GRCm38)	missense	probably damaging	1.00
R5686:Brca2	UTSW	5	150,540,904 (GRCm38)	missense	probably benign	0.00
R5730:Brca2	UTSW	5	150,569,005 (GRCm38)	missense	possibly damaging	0.85
R5763:Brca2	UTSW	5	150,548,006 (GRCm38)	missense	possibly damaging	0.85
R5877:Brca2	UTSW	5	150,543,221 (GRCm38)	missense	possibly damaging	0.53
R5893:Brca2	UTSW	5	150,569,138 (GRCm38)	missense	probably benign	0.02
R5900:Brca2	UTSW	5	150,541,132 (GRCm38)	missense	probably benign	0.01
R5926:Brca2	UTSW	5	150,534,622 (GRCm38)	missense	probably benign	0.07
R5966:Brca2	UTSW	5	150,543,251 (GRCm38)	missense	probably damaging	0.99
R6025:Brca2	UTSW	5	150,541,575 (GRCm38)	frame shift	probably null
R6062:Brca2	UTSW	5	150,556,889 (GRCm38)	missense	probably damaging	0.96
R6141:Brca2	UTSW	5	150,540,637 (GRCm38)	missense	possibly damaging	0.91
R6244:Brca2	UTSW	5	150,566,978 (GRCm38)	missense	probably benign	0.08
R6508:Brca2	UTSW	5	150,536,593 (GRCm38)	missense	possibly damaging	0.91
R6519:Brca2	UTSW	5	150,540,979 (GRCm38)	missense	probably damaging	0.99
R6611:Brca2	UTSW	5	150,536,193 (GRCm38)	missense	probably damaging	0.99
R6698:Brca2	UTSW	5	150,532,394 (GRCm38)	missense	probably damaging	1.00
R6856:Brca2	UTSW	5	150,540,208 (GRCm38)	missense	possibly damaging	0.68
R6912:Brca2	UTSW	5	150,541,742 (GRCm38)	missense	probably damaging	0.99
R7002:Brca2	UTSW	5	150,539,918 (GRCm38)	missense	probably benign
R7025:Brca2	UTSW	5	150,540,478 (GRCm38)	missense	probably benign	0.39
R7151:Brca2	UTSW	5	150,541,436 (GRCm38)	missense	probably benign	0.12
R7202:Brca2	UTSW	5	150,532,354 (GRCm38)	missense	probably benign	0.03
R7365:Brca2	UTSW	5	150,532,337 (GRCm38)	missense	probably damaging	0.99
R7510:Brca2	UTSW	5	150,536,691 (GRCm38)	missense	possibly damaging	0.85
R7612:Brca2	UTSW	5	150,540,611 (GRCm38)	missense	probably benign	0.03
R7682:Brca2	UTSW	5	150,543,153 (GRCm38)	missense	probably benign
R7890:Brca2	UTSW	5	150,539,381 (GRCm38)	missense	possibly damaging	0.83
R7930:Brca2	UTSW	5	150,558,510 (GRCm38)	missense	probably damaging	0.96
R7940:Brca2	UTSW	5	150,538,733 (GRCm38)	missense	probably benign
R8054:Brca2	UTSW	5	150,536,504 (GRCm38)	missense	probably benign	0.02
R8080:Brca2	UTSW	5	150,539,892 (GRCm38)	missense	probably benign	0.11
R8094:Brca2	UTSW	5	150,536,169 (GRCm38)	missense	possibly damaging	0.85
R8306:Brca2	UTSW	5	150,536,663 (GRCm38)	missense	possibly damaging	0.91
R8401:Brca2	UTSW	5	150,552,352 (GRCm38)	missense	probably damaging	1.00
R8523:Brca2	UTSW	5	150,560,148 (GRCm38)	missense	possibly damaging	0.75
R8784:Brca2	UTSW	5	150,548,661 (GRCm38)	nonsense	probably null
R8791:Brca2	UTSW	5	150,542,596 (GRCm38)	missense	possibly damaging	0.92
R8832:Brca2	UTSW	5	150,542,146 (GRCm38)	missense	possibly damaging	0.91
R8838:Brca2	UTSW	5	150,541,540 (GRCm38)	missense	possibly damaging	0.91
R8845:Brca2	UTSW	5	150,543,382 (GRCm38)	missense	possibly damaging	0.85
R8898:Brca2	UTSW	5	150,569,033 (GRCm38)	missense	possibly damaging	0.53
R8914:Brca2	UTSW	5	150,541,743 (GRCm38)	missense	probably damaging	0.96
R8935:Brca2	UTSW	5	150,568,981 (GRCm38)	missense	possibly damaging	0.70
R9014:Brca2	UTSW	5	150,541,754 (GRCm38)	missense	probably benign
R9023:Brca2	UTSW	5	150,541,895 (GRCm38)	missense	probably benign	0.07
R9094:Brca2	UTSW	5	150,552,305 (GRCm38)	missense	probably benign	0.08
R9195:Brca2	UTSW	5	150,539,953 (GRCm38)	missense	possibly damaging	0.83
R9198:Brca2	UTSW	5	150,536,512 (GRCm38)	missense	possibly damaging	0.91
R9314:Brca2	UTSW	5	150,550,894 (GRCm38)	missense	probably damaging	0.96
R9408:Brca2	UTSW	5	150,541,517 (GRCm38)	missense	probably damaging	1.00
R9459:Brca2	UTSW	5	150,540,629 (GRCm38)	missense	probably damaging	0.98
R9512:Brca2	UTSW	5	150,531,081 (GRCm38)	missense	probably benign	0.40
R9622:Brca2	UTSW	5	150,556,945 (GRCm38)	missense	probably damaging	0.96
R9777:Brca2	UTSW	5	150,557,114 (GRCm38)	missense	probably damaging	0.99
Z1088:Brca2	UTSW	5	150,542,763 (GRCm38)	missense	probably damaging	0.96
Z1186:Brca2	UTSW	5	150,536,583 (GRCm38)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TGGATTTCCTAAGTCGGCTGC -3'
(R):5'- CAACAGCTAATTTCTCACTGCTG -3'

Sequencing Primer
(F):5'- TAAGTCGGCTGCCCTTACCG -3'
(R):5'- GTCGCCTCTACAGTCCCTGAG -3'

Posted On

2020-01-23