Incidental Mutation 'R8058:Orc3'
ID 619512
Institutional Source Beutler Lab
Gene Symbol Orc3
Ensembl Gene ENSMUSG00000040044
Gene Name origin recognition complex, subunit 3
Synonyms Orc3l
MMRRC Submission 067495-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R8058 (G1)
Quality Score 225.009
Status Not validated
Chromosome 4
Chromosomal Location 34570796-34614944 bp(-) (GRCm39)
Type of Mutation nonsense
DNA Base Change (assembly) C to A at 34595223 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glutamic Acid to Stop codon at position 249 (E249*)
Ref Sequence ENSEMBL: ENSMUSP00000048319 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000048706] [ENSMUST00000108142] [ENSMUST00000140334]
AlphaFold Q9JK30
Predicted Effect probably null
Transcript: ENSMUST00000048706
AA Change: E249*
SMART Domains Protein: ENSMUSP00000048319
Gene: ENSMUSG00000040044
AA Change: E249*

DomainStartEndE-ValueType
Pfam:ORC3_N 25 350 3e-130 PFAM
low complexity region 653 664 N/A INTRINSIC
Predicted Effect probably null
Transcript: ENSMUST00000108142
AA Change: E249*
SMART Domains Protein: ENSMUSP00000103777
Gene: ENSMUSG00000040044
AA Change: E249*

DomainStartEndE-ValueType
Pfam:ORC3_N 24 350 7.7e-136 PFAM
low complexity region 652 663 N/A INTRINSIC
Predicted Effect probably null
Transcript: ENSMUST00000140334
AA Change: E191*
SMART Domains Protein: ENSMUSP00000119335
Gene: ENSMUSG00000040044
AA Change: E191*

DomainStartEndE-ValueType
Pfam:ORC3_N 2 278 1.4e-117 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.6%
  • 20x: 98.8%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Studies of a similar gene in Drosophila suggested a possible role of this protein in neuronal proliferation and olfactory memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a conditional allele activated in the neural cells exhibit reduced neuronal precursor proliferation and reduced radial glial cell. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 78 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca1 C T 4: 53,081,954 (GRCm39) D769N possibly damaging Het
Abcb6 A G 1: 75,156,653 (GRCm39) L37P possibly damaging Het
Abcg1 G T 17: 31,324,504 (GRCm39) A250S probably benign Het
Acsf3 C T 8: 123,540,373 (GRCm39) H524Y possibly damaging Het
Agbl4 A G 4: 110,518,039 (GRCm39) K110R unknown Het
Arfgap2 G A 2: 91,096,644 (GRCm39) probably null Het
B4galt4 T C 16: 38,586,383 (GRCm39) probably null Het
Bex6 G T 16: 32,005,224 (GRCm39) D11Y probably damaging Het
Bhlhe40 TG TGG 6: 108,641,818 (GRCm39) 254 probably null Het
C8b A T 4: 104,647,811 (GRCm39) D334V probably damaging Het
Capn11 T C 17: 45,954,681 (GRCm39) Q152R probably null Het
Ccdc171 C A 4: 83,499,003 (GRCm39) Q234K probably damaging Het
Cemip2 C A 19: 21,829,695 (GRCm39) A1268E probably benign Het
Clec2h A G 6: 128,650,966 (GRCm39) D82G probably benign Het
Creld2 A G 15: 88,710,632 (GRCm39) D349G probably damaging Het
D430041D05Rik A G 2: 103,979,128 (GRCm39) *1415Q probably null Het
Dnmt3a T C 12: 3,952,768 (GRCm39) V755A possibly damaging Het
Doc2a G T 7: 126,450,164 (GRCm39) V201L probably benign Het
Fan1 T A 7: 64,022,234 (GRCm39) N340Y probably damaging Het
Fbn1 G T 2: 125,193,889 (GRCm39) D1359E possibly damaging Het
Fcnb T C 2: 27,969,707 (GRCm39) Y120C probably damaging Het
Fras1 T A 5: 96,842,778 (GRCm39) D1665E probably benign Het
Fry A T 5: 150,419,232 (GRCm39) D524V Het
Garem1 A G 18: 21,281,621 (GRCm39) L245P probably damaging Het
Gdf6 A G 4: 9,859,712 (GRCm39) S265G probably benign Het
Gm5591 T C 7: 38,218,363 (GRCm39) I837V probably benign Het
Gpr61 T C 3: 108,058,211 (GRCm39) Y150C probably damaging Het
Grin2b A T 6: 135,710,225 (GRCm39) L1107Q probably damaging Het
Helb A G 10: 119,941,483 (GRCm39) S402P probably benign Het
Htt A G 5: 34,977,444 (GRCm39) T777A probably benign Het
Iffo2 A G 4: 139,341,164 (GRCm39) D383G probably benign Het
Ikzf3 G T 11: 98,407,753 (GRCm39) Y29* probably null Het
Ints2 A T 11: 86,146,179 (GRCm39) M143K probably benign Het
Jmjd1c T C 10: 67,090,274 (GRCm39) V2292A not run Het
Katnip A G 7: 125,442,188 (GRCm39) E725G probably benign Het
Lamb1 C A 12: 31,353,046 (GRCm39) Q916K probably benign Het
Lrrtm4 G A 6: 79,999,528 (GRCm39) M313I probably benign Het
Lum A G 10: 97,404,425 (GRCm39) I107V probably benign Het
Ly6h T C 15: 75,437,061 (GRCm39) E126G probably benign Het
Map3k5 G A 10: 20,007,860 (GRCm39) V1230M probably damaging Het
Mcoln1 T C 8: 3,558,378 (GRCm39) F211L probably benign Het
Mink1 G A 11: 70,494,594 (GRCm39) W258* probably null Het
Muc16 G T 9: 18,571,298 (GRCm39) S407* probably null Het
Ngef G A 1: 87,473,744 (GRCm39) Q13* probably null Het
Nxpe5 T A 5: 138,237,573 (GRCm39) Y44* probably null Het
Or13d1 T A 4: 52,971,106 (GRCm39) L162M probably benign Het
Or4a77 A G 2: 89,487,671 (GRCm39) V38A probably benign Het
Or5t9 A G 2: 86,660,052 (GRCm39) R319G probably benign Het
Or7e178 T A 9: 20,225,476 (GRCm39) I247F probably damaging Het
Or8g24 A T 9: 38,989,862 (GRCm39) Y60N probably damaging Het
Or8h8 C A 2: 86,753,151 (GRCm39) A242S probably benign Het
Otogl A T 10: 107,598,287 (GRCm39) C2288S probably damaging Het
Pde1b A G 15: 103,433,238 (GRCm39) E249G probably damaging Het
Phf2 T C 13: 48,976,558 (GRCm39) E219G unknown Het
Rbm19 T A 5: 120,278,440 (GRCm39) probably null Het
Rcc2 G A 4: 140,429,586 (GRCm39) C40Y probably benign Het
Sbk2 A G 7: 4,960,289 (GRCm39) Y294H possibly damaging Het
Selenoo T C 15: 88,976,942 (GRCm39) I198T possibly damaging Het
Sema3f T C 9: 107,559,800 (GRCm39) E729G probably benign Het
Serpinb1b A T 13: 33,269,293 (GRCm39) T9S probably benign Het
Sfxn4 T C 19: 60,832,690 (GRCm39) T235A probably damaging Het
Shc4 A T 2: 125,491,154 (GRCm39) Y461* probably null Het
Slc22a15 T C 3: 101,771,926 (GRCm39) D391G probably benign Het
Slc22a23 G T 13: 34,489,167 (GRCm39) Y239* probably null Het
Slc24a2 G A 4: 86,909,750 (GRCm39) A656V probably damaging Het
Slc2a5 A T 4: 150,227,590 (GRCm39) I470F probably damaging Het
Sptbn4 T C 7: 27,063,694 (GRCm39) E2249G possibly damaging Het
Tas2r139 A T 6: 42,118,753 (GRCm39) Q295L probably benign Het
Tdrd7 C A 4: 46,034,309 (GRCm39) S1051R probably benign Het
Tnfrsf18 G A 4: 156,112,802 (GRCm39) A163T probably benign Het
Ube3b T C 5: 114,544,846 (GRCm39) V596A possibly damaging Het
Vmn2r10 T A 5: 109,149,955 (GRCm39) N363I probably benign Het
Vmn2r15 T A 5: 109,440,956 (GRCm39) I301F probably damaging Het
Yod1 C T 1: 130,646,806 (GRCm39) Q228* probably null Het
Zfp433 A T 10: 81,556,124 (GRCm39) K209* probably null Het
Zfp865 G A 7: 5,033,445 (GRCm39) V477I probably benign Het
Zfp937 T A 2: 150,081,421 (GRCm39) Y484N probably benign Het
Zfp951 T G 5: 104,962,312 (GRCm39) H418P probably damaging Het
Other mutations in Orc3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01636:Orc3 APN 4 34,595,096 (GRCm39) missense probably damaging 1.00
IGL03293:Orc3 APN 4 34,595,210 (GRCm39) missense probably damaging 0.96
IGL02991:Orc3 UTSW 4 34,593,083 (GRCm39) missense probably damaging 1.00
R0157:Orc3 UTSW 4 34,607,130 (GRCm39) critical splice donor site probably null
R0708:Orc3 UTSW 4 34,597,368 (GRCm39) missense probably damaging 1.00
R1331:Orc3 UTSW 4 34,599,748 (GRCm39) missense probably benign 0.01
R1481:Orc3 UTSW 4 34,607,228 (GRCm39) missense possibly damaging 0.50
R1755:Orc3 UTSW 4 34,575,114 (GRCm39) missense possibly damaging 0.67
R1886:Orc3 UTSW 4 34,584,829 (GRCm39) missense probably damaging 1.00
R2008:Orc3 UTSW 4 34,611,049 (GRCm39) splice site probably null
R2054:Orc3 UTSW 4 34,584,846 (GRCm39) missense probably damaging 0.97
R2307:Orc3 UTSW 4 34,586,503 (GRCm39) missense probably damaging 1.00
R3001:Orc3 UTSW 4 34,571,790 (GRCm39) missense probably benign 0.10
R3002:Orc3 UTSW 4 34,571,790 (GRCm39) missense probably benign 0.10
R3153:Orc3 UTSW 4 34,575,124 (GRCm39) missense probably damaging 0.99
R4044:Orc3 UTSW 4 34,587,055 (GRCm39) nonsense probably null
R4814:Orc3 UTSW 4 34,572,450 (GRCm39) splice site probably benign
R4825:Orc3 UTSW 4 34,571,774 (GRCm39) missense possibly damaging 0.95
R4939:Orc3 UTSW 4 34,593,126 (GRCm39) nonsense probably null
R6314:Orc3 UTSW 4 34,579,797 (GRCm39) missense possibly damaging 0.85
R6867:Orc3 UTSW 4 34,605,539 (GRCm39) missense probably damaging 1.00
R7227:Orc3 UTSW 4 34,572,542 (GRCm39) missense probably benign 0.00
R7417:Orc3 UTSW 4 34,595,136 (GRCm39) missense probably damaging 1.00
R7655:Orc3 UTSW 4 34,587,032 (GRCm39) nonsense probably null
R7656:Orc3 UTSW 4 34,587,032 (GRCm39) nonsense probably null
R7707:Orc3 UTSW 4 34,598,691 (GRCm39) nonsense probably null
R7856:Orc3 UTSW 4 34,585,647 (GRCm39) missense probably benign
R7967:Orc3 UTSW 4 34,598,645 (GRCm39) missense probably damaging 0.98
R8443:Orc3 UTSW 4 34,593,173 (GRCm39) missense probably damaging 1.00
R8670:Orc3 UTSW 4 34,572,529 (GRCm39) missense probably damaging 1.00
R8738:Orc3 UTSW 4 34,599,778 (GRCm39) missense possibly damaging 0.91
R8827:Orc3 UTSW 4 34,605,569 (GRCm39) missense probably benign 0.01
R9303:Orc3 UTSW 4 34,607,181 (GRCm39) nonsense probably null
R9305:Orc3 UTSW 4 34,607,181 (GRCm39) nonsense probably null
R9684:Orc3 UTSW 4 34,607,135 (GRCm39) missense probably benign 0.01
Predicted Primers PCR Primer
(F):5'- GTTTTACTCTGTCATTGGTAAGCAC -3'
(R):5'- CAGCAGGAGCATTTGGTCTTTC -3'

Sequencing Primer
(F):5'- ACTCTGTCATTGGTAAGCACTTTTTC -3'
(R):5'- GCATTCATTAATGGCTTGATTTCAGG -3'
Posted On 2020-01-23