Mutagenetix > Incidental Mutations

Incidental Mutation 'R8070:Madd'

620208

Institutional Source

Beutler Lab

Gene Symbol

Madd

Ensembl Gene

ENSMUSG00000040687

Gene Name

MAP-kinase activating death domain

Synonyms

Rab3 GEP, 9630059K23Rik

MMRRC Submission

Accession Numbers

Is this an essential gene?

Essential (E-score: 1.000) question?

Stock #

R8070 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

91137360-91183837 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 91158014 bp

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Stop codon at position 1223 (E1223*)

Ref Sequence

ENSEMBL: ENSMUSP00000107001 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000066420] [ENSMUST00000066473] [ENSMUST00000075269] [ENSMUST00000077941] [ENSMUST00000099723] [ENSMUST00000099725] [ENSMUST00000111369] [ENSMUST00000111370] [ENSMUST00000111371] [ENSMUST00000111372] [ENSMUST00000111373] [ENSMUST00000111375] [ENSMUST00000111376] [ENSMUST00000111381]

Predicted Effect

probably benign
Transcript: ENSMUST00000066420

SMART Domains

Protein: ENSMUSP00000067210
Gene: ENSMUSG00000040687

Domain	Start	End	E-Value	Type
uDENN	7	97	7.11e-26	SMART
low complexity region	124	139	N/A	INTRINSIC
DENN	171	401	2.19e-67	SMART
low complexity region	412	428	N/A	INTRINSIC
dDENN	484	554	6.71e-13	SMART
low complexity region	619	639	N/A	INTRINSIC
low complexity region	699	718	N/A	INTRINSIC
low complexity region	781	789	N/A	INTRINSIC
low complexity region	816	839	N/A	INTRINSIC
low complexity region	908	918	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000066473
AA Change: E1223*

SMART Domains

Protein: ENSMUSP00000069350
Gene: ENSMUSG00000040687
AA Change: E1223*

Domain	Start	End	E-Value	Type
uDENN	7	97	7.11e-26	SMART
low complexity region	124	139	N/A	INTRINSIC
DENN	171	401	2.19e-67	SMART
low complexity region	412	428	N/A	INTRINSIC
dDENN	484	554	6.71e-13	SMART
low complexity region	619	639	N/A	INTRINSIC
low complexity region	699	718	N/A	INTRINSIC
low complexity region	781	789	N/A	INTRINSIC
low complexity region	816	839	N/A	INTRINSIC
low complexity region	908	918	N/A	INTRINSIC
low complexity region	1334	1348	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000075269
AA Change: E1186*

SMART Domains

Protein: ENSMUSP00000074746
Gene: ENSMUSG00000040687
AA Change: E1186*

Domain	Start	End	E-Value	Type
uDENN	7	97	7.11e-26	SMART
low complexity region	124	139	N/A	INTRINSIC
DENN	171	401	2.19e-67	SMART
low complexity region	412	428	N/A	INTRINSIC
dDENN	484	554	6.71e-13	SMART
low complexity region	619	639	N/A	INTRINSIC
low complexity region	699	719	N/A	INTRINSIC
low complexity region	762	770	N/A	INTRINSIC
low complexity region	797	820	N/A	INTRINSIC
low complexity region	889	899	N/A	INTRINSIC
low complexity region	1276	1290	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000077941
AA Change: E1243*

SMART Domains

Protein: ENSMUSP00000077094
Gene: ENSMUSG00000040687
AA Change: E1243*

Domain	Start	End	E-Value	Type
uDENN	7	97	7.11e-26	SMART
low complexity region	124	139	N/A	INTRINSIC
DENN	171	401	2.19e-67	SMART
low complexity region	412	428	N/A	INTRINSIC
dDENN	484	554	6.71e-13	SMART
low complexity region	619	639	N/A	INTRINSIC
low complexity region	699	718	N/A	INTRINSIC
low complexity region	781	789	N/A	INTRINSIC
low complexity region	816	839	N/A	INTRINSIC
low complexity region	928	938	N/A	INTRINSIC
low complexity region	1354	1368	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000099723
AA Change: E1242*

SMART Domains

Protein: ENSMUSP00000097311
Gene: ENSMUSG00000040687
AA Change: E1242*

Domain	Start	End	E-Value	Type
uDENN	7	97	7.11e-26	SMART
low complexity region	124	139	N/A	INTRINSIC
DENN	171	401	2.19e-67	SMART
low complexity region	412	428	N/A	INTRINSIC
dDENN	484	554	6.71e-13	SMART
low complexity region	619	639	N/A	INTRINSIC
low complexity region	699	718	N/A	INTRINSIC
low complexity region	781	789	N/A	INTRINSIC
low complexity region	816	839	N/A	INTRINSIC
low complexity region	928	938	N/A	INTRINSIC
low complexity region	1189	1203	N/A	INTRINSIC
low complexity region	1353	1367	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000099725
AA Change: E1223*

SMART Domains

Protein: ENSMUSP00000097313
Gene: ENSMUSG00000040687
AA Change: E1223*

Domain	Start	End	E-Value	Type
uDENN	7	97	7.11e-26	SMART
low complexity region	124	139	N/A	INTRINSIC
DENN	171	401	2.19e-67	SMART
low complexity region	412	428	N/A	INTRINSIC
dDENN	484	554	6.71e-13	SMART
low complexity region	619	639	N/A	INTRINSIC
low complexity region	699	718	N/A	INTRINSIC
low complexity region	781	789	N/A	INTRINSIC
low complexity region	816	839	N/A	INTRINSIC
low complexity region	908	918	N/A	INTRINSIC
low complexity region	1334	1348	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000111369
AA Change: E1161*

SMART Domains

Protein: ENSMUSP00000107000
Gene: ENSMUSG00000040687
AA Change: E1161*

Domain	Start	End	E-Value	Type
uDENN	7	97	7.11e-26	SMART
low complexity region	124	139	N/A	INTRINSIC
DENN	171	401	2.19e-67	SMART
low complexity region	412	428	N/A	INTRINSIC
dDENN	484	554	6.71e-13	SMART
low complexity region	619	639	N/A	INTRINSIC
low complexity region	699	721	N/A	INTRINSIC
low complexity region	773	796	N/A	INTRINSIC
low complexity region	865	875	N/A	INTRINSIC
low complexity region	1108	1122	N/A	INTRINSIC
low complexity region	1251	1265	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000111370
AA Change: E1223*

SMART Domains

Protein: ENSMUSP00000107001
Gene: ENSMUSG00000040687
AA Change: E1223*

Domain	Start	End	E-Value	Type
uDENN	7	97	7.11e-26	SMART
low complexity region	124	139	N/A	INTRINSIC
DENN	171	401	2.19e-67	SMART
low complexity region	412	428	N/A	INTRINSIC
dDENN	484	554	6.71e-13	SMART
low complexity region	619	639	N/A	INTRINSIC
low complexity region	699	718	N/A	INTRINSIC
low complexity region	781	789	N/A	INTRINSIC
low complexity region	816	839	N/A	INTRINSIC
low complexity region	908	918	N/A	INTRINSIC
low complexity region	1334	1348	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000111371
AA Change: E1206*

SMART Domains

Protein: ENSMUSP00000107002
Gene: ENSMUSG00000040687
AA Change: E1206*

Domain	Start	End	E-Value	Type
uDENN	7	97	7.11e-26	SMART
low complexity region	124	139	N/A	INTRINSIC
DENN	171	401	2.19e-67	SMART
low complexity region	412	428	N/A	INTRINSIC
dDENN	484	554	6.71e-13	SMART
low complexity region	619	639	N/A	INTRINSIC
low complexity region	699	719	N/A	INTRINSIC
low complexity region	762	770	N/A	INTRINSIC
low complexity region	797	820	N/A	INTRINSIC
low complexity region	909	919	N/A	INTRINSIC
low complexity region	1296	1310	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000111372
AA Change: E1205*

SMART Domains

Protein: ENSMUSP00000107003
Gene: ENSMUSG00000040687
AA Change: E1205*

Domain	Start	End	E-Value	Type
uDENN	7	97	7.11e-26	SMART
low complexity region	124	139	N/A	INTRINSIC
DENN	171	401	2.19e-67	SMART
low complexity region	412	428	N/A	INTRINSIC
dDENN	484	554	6.71e-13	SMART
low complexity region	619	639	N/A	INTRINSIC
low complexity region	699	718	N/A	INTRINSIC
low complexity region	781	789	N/A	INTRINSIC
low complexity region	816	839	N/A	INTRINSIC
low complexity region	908	918	N/A	INTRINSIC
low complexity region	1295	1309	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000111373
AA Change: E1161*

SMART Domains

Protein: ENSMUSP00000107004
Gene: ENSMUSG00000040687
AA Change: E1161*

Domain	Start	End	E-Value	Type
uDENN	7	97	2.9e-29	SMART
low complexity region	124	139	N/A	INTRINSIC
DENN	171	401	8.7e-71	SMART
low complexity region	412	428	N/A	INTRINSIC
dDENN	484	554	2.8e-16	SMART
low complexity region	619	639	N/A	INTRINSIC
low complexity region	699	721	N/A	INTRINSIC
low complexity region	773	796	N/A	INTRINSIC
low complexity region	865	875	N/A	INTRINSIC
low complexity region	1108	1122	N/A	INTRINSIC
low complexity region	1251	1265	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000111375
AA Change: E1182*

SMART Domains

Protein: ENSMUSP00000107006
Gene: ENSMUSG00000040687
AA Change: E1182*

Domain	Start	End	E-Value	Type
uDENN	7	97	7.11e-26	SMART
low complexity region	124	139	N/A	INTRINSIC
DENN	171	401	2.19e-67	SMART
low complexity region	412	428	N/A	INTRINSIC
dDENN	484	554	6.71e-13	SMART
low complexity region	619	639	N/A	INTRINSIC
low complexity region	699	721	N/A	INTRINSIC
low complexity region	773	796	N/A	INTRINSIC
low complexity region	885	895	N/A	INTRINSIC
low complexity region	1272	1286	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000111376
AA Change: E1201*

SMART Domains

Protein: ENSMUSP00000107007
Gene: ENSMUSG00000040687
AA Change: E1201*

Domain	Start	End	E-Value	Type
uDENN	7	97	7.11e-26	SMART
low complexity region	124	139	N/A	INTRINSIC
DENN	171	401	2.19e-67	SMART
low complexity region	412	428	N/A	INTRINSIC
dDENN	484	554	6.71e-13	SMART
low complexity region	619	639	N/A	INTRINSIC
low complexity region	699	721	N/A	INTRINSIC
low complexity region	781	789	N/A	INTRINSIC
low complexity region	816	839	N/A	INTRINSIC
low complexity region	908	918	N/A	INTRINSIC
low complexity region	1151	1162	N/A	INTRINSIC
low complexity region	1312	1326	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000111381
AA Change: E1225*

SMART Domains

Protein: ENSMUSP00000107012
Gene: ENSMUSG00000040687
AA Change: E1225*

Domain	Start	End	E-Value	Type
uDENN	7	97	7.11e-26	SMART
low complexity region	124	139	N/A	INTRINSIC
DENN	171	401	2.19e-67	SMART
low complexity region	412	428	N/A	INTRINSIC
dDENN	484	554	6.71e-13	SMART
low complexity region	619	639	N/A	INTRINSIC
low complexity region	699	718	N/A	INTRINSIC
low complexity region	781	789	N/A	INTRINSIC
low complexity region	816	839	N/A	INTRINSIC
low complexity region	928	938	N/A	INTRINSIC
low complexity region	1315	1329	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. The protein encoded by this gene is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele die shortly after birth due to respiratory failure, are hyporesponsive to tactile stimuli, and exhibit defects in neurotransmitter release with impaired synaptic vesicle trafficking and depletion of synaptic vesicles at the neuromuscular junction. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610040J01Rik	A	G	5: 63,898,167	E82G	probably benign	Het
9830107B12Rik	A	G	17: 48,145,510	F86S	probably damaging	Het
Aamdc	A	T	7: 97,575,648	Y2*	probably null	Het
Acta1	T	A	8: 123,893,621	D26V	possibly damaging	Het
Adra1d	A	C	2: 131,561,582	L196R	probably damaging	Het
Agbl2	G	A	2: 90,791,565	C132Y	probably benign	Het
Amd1	A	G	10: 40,294,230	V92A	probably benign	Het
Arid4b	T	A	13: 14,136,259	I149K	probably benign	Het
Atg7	A	G	6: 114,697,080	M312V	probably benign	Het
Bap1	T	A	14: 31,256,686	V381E	probably damaging	Het
Cbfa2t3	C	T	8: 122,642,981	V207I	possibly damaging	Het
Cdh24	T	C	14: 54,632,573	Q629R	probably benign	Het
Cdhr2	G	T	13: 54,719,793	V479L	probably benign	Het
Chd2	A	G	7: 73,451,758	S1407P	probably benign	Het
Clstn2	A	G	9: 97,799,470	V39A	possibly damaging	Het
Cwh43	T	A	5: 73,421,463	M357K	possibly damaging	Het
Dcdc2a	T	A	13: 25,202,197	D351E	probably benign	Het
Dennd6b	T	C	15: 89,185,373	I517V	probably benign	Het
Dnah17	T	C	11: 118,024,671	E4374G	probably damaging	Het
Emsy	A	G	7: 98,626,715	S336P	possibly damaging	Het
Enoph1	A	T	5: 100,060,982	E65D	probably benign	Het
Fam83f	T	A	15: 80,672,080	L55Q	probably damaging	Het
Fry	T	C	5: 150,478,007	F379L		Het
Fscb	A	T	12: 64,474,608	M28K	probably benign	Het
Gas7	G	A	11: 67,683,434	V412M	probably damaging	Het
Gatad1	T	C	5: 3,643,540	R210G	probably benign	Het
Gcn1l1	T	A	5: 115,588,998	V638E	probably benign	Het
Ggt1	A	T	10: 75,578,899	I184F	probably damaging	Het
Gigyf2	A	G	1: 87,440,907	N1103S	probably benign	Het
Gm13757	G	T	2: 88,446,659	T93K	probably benign	Het
Gm5592	G	A	7: 41,286,463	A130T	possibly damaging	Het
Gys2	T	C	6: 142,448,504		probably null	Het
Hmcn1	C	A	1: 150,649,992	E3327*	probably null	Het
Ighv1-36	C	T	12: 114,880,036	G68E	probably damaging	Het
Igkv6-32	G	A	6: 70,074,105	T89M	probably damaging	Het
Ipo7	A	G	7: 110,052,807	D931G	probably benign	Het
Jakmip1	A	G	5: 37,173,287	E437G	probably benign	Het
Lingo3	C	T	10: 80,836,121		probably benign	Het
Lnpep	A	G	17: 17,538,638	S815P	probably damaging	Het
Ly6a	T	C	15: 74,997,600	D2G	probably damaging	Het
Mapk8ip3	A	T	17: 24,901,104		probably null	Het
Mecom	T	C	3: 29,979,838	E239G	probably damaging	Het
Mug1	T	C	6: 121,875,879	V889A	probably benign	Het
Myo18b	T	A	5: 112,791,120	N1675I	probably benign	Het
Ndrg4	T	C	8: 95,700,128	F50L	possibly damaging	Het
Ndst4	A	G	3: 125,714,644	Y286C	probably damaging	Het
Nrp2	C	T	1: 62,745,408	R239C	probably damaging	Het
Olfm3	A	G	3: 115,101,955	D195G	probably damaging	Het
Olfr1384	A	T	11: 49,514,114	T159S	probably damaging	Het
Pds5a	A	G	5: 65,652,398	L407P	possibly damaging	Het
Pgm1	A	G	5: 64,112,082	N504S	probably benign	Het
Plce1	A	G	19: 38,701,839	M656V	probably damaging	Het
Pou6f2	T	C	13: 18,239,624	T189A	unknown	Het
Ppp1r12b	T	C	1: 134,876,069	S451G	probably benign	Het
Prox1	T	G	1: 190,160,910	N446T	probably damaging	Het
Ralgapa2	T	A	2: 146,353,279	R1195S	probably damaging	Het
Rere	A	G	4: 150,617,375	D37G	probably damaging	Het
Tfip11	T	A	5: 112,334,930	M560K	possibly damaging	Het
Thop1	T	C	10: 81,079,486	V260A	probably damaging	Het
Tle6	A	G	10: 81,598,642	M41T	possibly damaging	Het
Trav13-4-dv7	T	C	14: 53,757,792	S68P	possibly damaging	Het
Trim24	A	G	6: 37,957,726	N826S	probably damaging	Het
Tuba3a	T	C	6: 125,278,470	E414G	probably damaging	Het
Vmn2r78	A	G	7: 86,922,487	I502V	probably benign	Het
Vmn2r79	A	T	7: 87,002,128	Y245F	probably benign	Het

Other mutations in Madd

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Madd	APN	2	91175766	unclassified	probably benign
IGL00781:Madd	APN	2	91146928	missense	probably benign	0.00
IGL00844:Madd	APN	2	91167868	missense	probably damaging	1.00
IGL00942:Madd	APN	2	91170578	missense	probably damaging	1.00
IGL01100:Madd	APN	2	91158040	missense	probably damaging	1.00
IGL01116:Madd	APN	2	91154543	splice site	probably benign
IGL01694:Madd	APN	2	91157975	splice site	probably benign
IGL01982:Madd	APN	2	91175707	missense	probably damaging	1.00
IGL02346:Madd	APN	2	91162491	missense	probably damaging	0.97
IGL02354:Madd	APN	2	91162198	missense	probably benign	0.17
IGL02361:Madd	APN	2	91162198	missense	probably benign	0.17
IGL02481:Madd	APN	2	91178036	missense	probably damaging	1.00
IGL02483:Madd	APN	2	91178036	missense	probably damaging	1.00
IGL02948:Madd	APN	2	91142827	missense	probably benign
IGL03338:Madd	APN	2	91162162	missense	possibly damaging	0.48
BB005:Madd	UTSW	2	91176888	missense	probably damaging	1.00
BB015:Madd	UTSW	2	91176888	missense	probably damaging	1.00
R0026:Madd	UTSW	2	91175708	missense	possibly damaging	0.88
R0026:Madd	UTSW	2	91175708	missense	possibly damaging	0.88
R0027:Madd	UTSW	2	91152549	missense	probably damaging	0.97
R0085:Madd	UTSW	2	91162738	missense	probably benign	0.00
R0577:Madd	UTSW	2	91138395	missense	possibly damaging	0.88
R0587:Madd	UTSW	2	91146885	missense	probably damaging	1.00
R1112:Madd	UTSW	2	91143599	missense	probably damaging	1.00
R1722:Madd	UTSW	2	91167637	missense	probably benign
R1750:Madd	UTSW	2	91167891	missense	probably damaging	0.98
R2061:Madd	UTSW	2	91161486	intron	probably benign
R2112:Madd	UTSW	2	91176976	missense	possibly damaging	0.89
R2114:Madd	UTSW	2	91164022	missense	probably damaging	1.00
R2140:Madd	UTSW	2	91152509	missense	possibly damaging	0.80
R2276:Madd	UTSW	2	91143683	missense	possibly damaging	0.67
R2277:Madd	UTSW	2	91143683	missense	possibly damaging	0.67
R2279:Madd	UTSW	2	91143683	missense	possibly damaging	0.67
R2424:Madd	UTSW	2	91166622	missense	probably damaging	1.00
R2904:Madd	UTSW	2	91175672	missense	probably damaging	1.00
R3122:Madd	UTSW	2	91176209	missense	probably damaging	1.00
R3836:Madd	UTSW	2	91154643	critical splice donor site	probably null
R3979:Madd	UTSW	2	91176828	missense	possibly damaging	0.81
R4151:Madd	UTSW	2	91143083	missense	probably benign	0.11
R4233:Madd	UTSW	2	91178236	missense	probably benign	0.26
R4236:Madd	UTSW	2	91167028	missense	probably benign	0.00
R4299:Madd	UTSW	2	91169803	missense	probably damaging	1.00
R4334:Madd	UTSW	2	91140572	missense	probably benign	0.08
R4413:Madd	UTSW	2	91167587	missense	probably damaging	1.00
R4595:Madd	UTSW	2	91167664	missense	possibly damaging	0.80
R4694:Madd	UTSW	2	91160328	missense	probably damaging	0.99
R5410:Madd	UTSW	2	91154514	missense	probably damaging	1.00
R5490:Madd	UTSW	2	91170635	missense	possibly damaging	0.80
R5560:Madd	UTSW	2	91163545	missense	probably damaging	1.00
R5661:Madd	UTSW	2	91154433	critical splice donor site	probably null
R5710:Madd	UTSW	2	91154476	missense	probably damaging	1.00
R5730:Madd	UTSW	2	91158109	missense	probably damaging	1.00
R5759:Madd	UTSW	2	91162075	missense	possibly damaging	0.94
R5768:Madd	UTSW	2	91167829	missense	probably damaging	1.00
R5822:Madd	UTSW	2	91152533	missense	probably damaging	1.00
R6125:Madd	UTSW	2	91152452	critical splice donor site	probably null
R6151:Madd	UTSW	2	91165457	nonsense	probably null
R6229:Madd	UTSW	2	91143670	missense	probably damaging	0.96
R6230:Madd	UTSW	2	91143521	critical splice donor site	probably null
R6245:Madd	UTSW	2	91178104	missense	probably benign	0.27
R6323:Madd	UTSW	2	91161438	splice site	probably null
R6456:Madd	UTSW	2	91178191	missense	probably benign
R6473:Madd	UTSW	2	91167059	missense	probably benign
R6878:Madd	UTSW	2	91169857	missense	probably damaging	1.00
R7060:Madd	UTSW	2	91177107	missense	probably damaging	1.00
R7065:Madd	UTSW	2	91155057	missense	probably benign	0.26
R7073:Madd	UTSW	2	91162509	missense	probably damaging	1.00
R7124:Madd	UTSW	2	91162048	missense	possibly damaging	0.94
R7251:Madd	UTSW	2	91162176	missense	probably benign	0.01
R7510:Madd	UTSW	2	91177976	missense	possibly damaging	0.80
R7605:Madd	UTSW	2	91169710	missense	possibly damaging	0.90
R7911:Madd	UTSW	2	91167508	missense	probably null	0.01
R7928:Madd	UTSW	2	91176888	missense	probably damaging	1.00
R7952:Madd	UTSW	2	91162541	missense	probably damaging	1.00
R8039:Madd	UTSW	2	91167061	missense	probably benign	0.17
R8047:Madd	UTSW	2	91179201	missense	probably damaging	1.00
R8048:Madd	UTSW	2	91154448	missense	probably damaging	0.99
R8090:Madd	UTSW	2	91155623	missense	probably benign	0.01
R8335:Madd	UTSW	2	91170239	missense	probably damaging	1.00
R8459:Madd	UTSW	2	91162526	missense	probably benign
R8678:Madd	UTSW	2	91176265	missense	probably damaging	1.00
X0067:Madd	UTSW	2	91152473	missense	probably damaging	1.00
Z1176:Madd	UTSW	2	91159272	missense	probably damaging	0.96
Z1177:Madd	UTSW	2	91142831	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCCTCAGGACTATGGGAAGG -3'
(R):5'- TAGCAGTCAGGGTTCTAGCTG -3'

Sequencing Primer
(F):5'- AGGCTCCCCTTTCCTCCG -3'
(R):5'- CTAGCTGGCTTTGGGAATCC -3'

Posted On

2020-01-23