Mutagenetix > Incidental Mutations

Incidental Mutation 'R0711:Zfp850'

62735

Institutional Source

Beutler Lab

Gene Symbol

Zfp850

Ensembl Gene

ENSMUSG00000096916

Gene Name

zinc finger protein 850

Synonyms

C130069I09Rik, Gm4636

MMRRC Submission

038894-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.066) question?

Stock #

R0711 (G1)

Quality Score

120

Status

Validated

Chromosome

Chromosomal Location

27984854-28014115 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 27990273 bp

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 170 (N170S)

Ref Sequence

ENSEMBL: ENSMUSP00000141063 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099111] [ENSMUST00000180024] [ENSMUST00000180502]

Predicted Effect

probably benign
Transcript: ENSMUST00000099111

Predicted Effect

probably benign
Transcript: ENSMUST00000180024
AA Change: N170S

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000137192
Gene: ENSMUSG00000096916
AA Change: N170S

Domain	Start	End	E-Value	Type
KRAB	14	75	1.56e-34	SMART
ZnF_C2H2	172	194	7.18e1	SMART
ZnF_C2H2	200	222	3.63e-3	SMART
ZnF_C2H2	228	250	8.94e-3	SMART
ZnF_C2H2	256	278	7.49e-5	SMART
ZnF_C2H2	313	335	1.01e-1	SMART
ZnF_C2H2	341	363	4.4e-2	SMART
ZnF_C2H2	369	391	7.37e-4	SMART
ZnF_C2H2	397	419	8.47e-4	SMART
ZnF_C2H2	425	447	1.92e-2	SMART
ZnF_C2H2	453	475	2.99e-4	SMART
ZnF_C2H2	481	503	7.78e-3	SMART
ZnF_C2H2	509	531	1.95e-3	SMART
ZnF_C2H2	537	559	1.92e-2	SMART
ZnF_C2H2	565	587	2.99e-4	SMART
ZnF_C2H2	593	615	1.79e-2	SMART
ZnF_C2H2	621	643	7.37e-4	SMART
ZnF_C2H2	649	671	4.4e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000180502
AA Change: N170S

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000141063
Gene: ENSMUSG00000096916
AA Change: N170S

Domain	Start	End	E-Value	Type
KRAB	14	75	6.5e-37	SMART
ZnF_C2H2	172	194	3e-1	SMART
ZnF_C2H2	200	222	1.5e-5	SMART
ZnF_C2H2	228	250	3.8e-5	SMART
ZnF_C2H2	256	274	2.5e-1	SMART

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.4%
20x: 91.4%

Validation Efficiency

100% (91/91)

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	T	A	3: 138,068,225	D1058E	probably damaging	Het
4933405L10Rik	A	T	8: 105,708,931		probably null	Het
Adamtsl3	T	A	7: 82,465,699		probably benign	Het
Afdn	C	T	17: 13,852,436	P874S	probably damaging	Het
Ankrd6	G	A	4: 32,815,326	A391V	probably damaging	Het
Arhgef28	T	G	13: 97,931,254	T1388P	probably damaging	Het
Asxl3	G	T	18: 22,524,451	M1839I	probably benign	Het
BC005537	T	C	13: 24,805,940	F129L	probably damaging	Het
Celf2	A	G	2: 6,721,415		probably null	Het
Chid1	C	T	7: 141,496,677	V325I	probably benign	Het
Cnn3	T	A	3: 121,449,984	D31E	probably benign	Het
Col12a1	G	A	9: 79,652,035	P1857L	probably damaging	Het
Cpeb1	T	A	7: 81,351,870	R430W	probably benign	Het
Daw1	T	C	1: 83,191,338		probably benign	Het
Dcaf13	A	G	15: 39,138,089	Y264C	probably damaging	Het
Dnah6	T	C	6: 73,087,602	I2666V	probably damaging	Het
Dnaic2	A	C	11: 114,754,332	D531A	probably benign	Het
Dock10	A	T	1: 80,523,975	F1833I	probably damaging	Het
Efhd2	C	T	4: 141,859,872	A200T	probably damaging	Het
Epb41l5	T	A	1: 119,623,911		probably benign	Het
Ermp1	A	G	19: 29,631,388	Y164H	possibly damaging	Het
Gkn2	T	C	6: 87,373,419		probably benign	Het
Golgb1	A	T	16: 36,918,790	Q2497L	probably damaging	Het
Gzme	A	T	14: 56,117,739	M245K	probably damaging	Het
Iars2	A	T	1: 185,322,388		probably benign	Het
Icosl	T	A	10: 78,073,941	V240D	probably damaging	Het
Igsf3	T	C	3: 101,427,393	M262T	probably benign	Het
Ing3	G	T	6: 21,971,237	E336*	probably null	Het
Kat2a	A	T	11: 100,706,471	V625E	probably damaging	Het
Ksr1	A	G	11: 79,038,247		probably benign	Het
Lypd8	A	T	11: 58,386,757	M122L	probably benign	Het
Mdfi	A	T	17: 47,832,930		probably benign	Het
Med13	A	G	11: 86,301,353		probably benign	Het
Msh6	C	T	17: 87,986,684	R956C	probably damaging	Het
Myo15b	A	G	11: 115,883,838	E670G	probably damaging	Het
Myo1d	A	G	11: 80,484,332	L972P	probably damaging	Het
Olfr1193	A	G	2: 88,678,674	D266G	probably damaging	Het
Olfr632	G	A	7: 103,937,817	A146T	probably benign	Het
Olfr834	T	A	9: 18,988,151	N54K	probably benign	Het
Pde8b	C	G	13: 95,107,817	S143T	possibly damaging	Het
Pias4	G	T	10: 81,157,530		probably benign	Het
Prkca	A	G	11: 107,981,654	Y427H	probably benign	Het
Psg25	G	A	7: 18,529,560	Q113*	probably null	Het
Rab3gap2	T	A	1: 185,249,926	S392T	probably damaging	Het
Scrib	A	G	15: 76,066,907		probably benign	Het
Sdk2	A	G	11: 113,903,144		probably benign	Het
Serpinb1c	T	A	13: 32,886,283		probably benign	Het
Serpinb9f	T	A	13: 33,327,921	W136R	probably damaging	Het
Skint10	C	A	4: 112,715,905		probably benign	Het
Slc25a13	T	C	6: 6,117,128	T196A	probably damaging	Het
Slc26a5	T	C	5: 21,847,232	H33R	probably damaging	Het
Slc27a6	T	C	18: 58,598,757		probably benign	Het
Slitrk6	A	T	14: 110,749,819	Y819N	probably damaging	Het
Spata46	C	T	1: 170,312,034	Q201*	probably null	Het
Sptbn1	A	T	11: 30,114,739	V1920E	probably damaging	Het
Taf6l	A	G	19: 8,778,517	F256L	probably benign	Het
Tmco3	T	A	8: 13,292,039	N104K	probably damaging	Het
Tmem200c	A	G	17: 68,842,254	T611A	probably damaging	Het
Tmem202	T	G	9: 59,525,372	Y24S	probably damaging	Het
Tpp1	A	G	7: 105,749,419	L230P	probably damaging	Het
Trim56	C	T	5: 137,112,992	E557K	probably benign	Het
Trrap	C	T	5: 144,853,499	L3590F	probably damaging	Het
Ttc37	C	T	13: 76,182,891	P1480L	probably damaging	Het
Tulp4	A	G	17: 6,139,112	T70A	possibly damaging	Het
Vcp	G	C	4: 42,986,201	A297G	probably benign	Het
Vwf	T	A	6: 125,626,271	H861Q	probably benign	Het
Wdr64	T	C	1: 175,772,185	I536T	probably benign	Het
Zfp72	G	A	13: 74,376,425		probably benign	Het

Other mutations in Zfp850

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02278:Zfp850	APN	7	28008397	missense	probably damaging	0.96
R0610:Zfp850	UTSW	7	27989394	missense	probably damaging	0.99
R0690:Zfp850	UTSW	7	27985217	missense	possibly damaging	0.67
R1310:Zfp850	UTSW	7	27989459	missense	probably benign	0.40
R1594:Zfp850	UTSW	7	27989391	missense	probably benign	0.00
R1771:Zfp850	UTSW	7	27985275	nonsense	probably null
R2189:Zfp850	UTSW	7	27989055	missense	probably benign	0.02
R2192:Zfp850	UTSW	7	27985195	missense	probably damaging	1.00
R2417:Zfp850	UTSW	7	27989183	missense	possibly damaging	0.54
R4321:Zfp850	UTSW	7	27989400	missense	probably damaging	0.99
R4770:Zfp850	UTSW	7	27984986	splice site	probably null
R4970:Zfp850	UTSW	7	27990233	nonsense	probably null
R5112:Zfp850	UTSW	7	27990233	nonsense	probably null
R5166:Zfp850	UTSW	7	27990356	nonsense	probably null
R5303:Zfp850	UTSW	7	28008413	missense	probably damaging	1.00
R5315:Zfp850	UTSW	7	27990318	missense	probably benign	0.02
R5496:Zfp850	UTSW	7	28007346	missense	probably damaging	0.98
R5547:Zfp850	UTSW	7	27989419	missense	probably damaging	1.00
R5677:Zfp850	UTSW	7	27989088	missense	probably damaging	1.00
R5927:Zfp850	UTSW	7	27990195	missense	probably benign	0.17
R6654:Zfp850	UTSW	7	27985215	nonsense	probably null
R6950:Zfp850	UTSW	7	27990514	missense	possibly damaging	0.86
R6987:Zfp850	UTSW	7	27990001	missense	probably damaging	1.00
R6990:Zfp850	UTSW	7	27990376	missense	probably benign	0.09
R7640:Zfp850	UTSW	7	27989209	missense	probably benign	0.05
R7856:Zfp850	UTSW	7	27990474	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- GCTGAGTGATGAGCTGTGCTAGAAC -3'
(R):5'- AGTGAAACTTTTGAACTCAAGGGCTCC -3'

Sequencing Primer
(F):5'- ggctttcccacattcattacattc -3'
(R):5'- GAACTCAAGGGCTCCAGTTTTAG -3'

Posted On

2013-07-30