Mutagenetix > Incidental Mutation

Incidental Mutation 'R7705:Fbxw24'

628348

Institutional Source

Beutler Lab

Gene Symbol

Fbxw24

Ensembl Gene

ENSMUSG00000062275

Gene Name

F-box and WD-40 domain protein 24

Synonyms

Gm5162

MMRRC Submission

045766-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.050) question?

Stock #

R7705 (G1)

Quality Score

101.008

Status

Validated

Chromosome

Chromosomal Location

109430184-109455125 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

A to T at 109437516 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000073617 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073962]

AlphaFold

E9PXM9

Predicted Effect

probably null
Transcript: ENSMUST00000073962

SMART Domains

Protein: ENSMUSP00000073617
Gene: ENSMUSG00000062275

Domain	Start	End	E-Value	Type
FBOX	8	45	3.25e-4	SMART
SCOP:d1gxra_	87	227	8e-7	SMART
Blast:WD40	137	176	1e-6	BLAST

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (48/48)

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl2fm3	G	A	3: 59,784,168 (GRCm39)	A214T	probably benign	Het
Abcb9	G	T	5: 124,220,018 (GRCm39)	Y342*	probably null	Het
Acad9	C	T	3: 36,142,675 (GRCm39)	T470M	probably benign	Het
Ankmy2	A	G	12: 36,245,107 (GRCm39)	E379G	probably benign	Het
Cand1	A	G	10: 119,048,343 (GRCm39)		probably null	Het
Ccdc171	A	G	4: 83,476,193 (GRCm39)	T203A	possibly damaging	Het
Cpeb4	A	G	11: 31,822,327 (GRCm39)	T14A	probably damaging	Het
Cplane1	T	G	15: 8,211,736 (GRCm39)	F359V	probably damaging	Het
Crtap	A	T	9: 114,210,747 (GRCm39)	C276S	probably damaging	Het
Csf2rb2	T	C	15: 78,168,774 (GRCm39)	I794V	probably benign	Het
Ctsf	T	G	19: 4,906,567 (GRCm39)	F165V	probably damaging	Het
Cux2	T	A	5: 122,007,736 (GRCm39)	M642L	probably benign	Het
Dpep1	T	C	8: 123,927,460 (GRCm39)	V338A	possibly damaging	Het
Dytn	A	G	1: 63,717,948 (GRCm39)	L29P	probably damaging	Het
E2f3	G	A	13: 30,169,306 (GRCm39)	R116C	probably benign	Het
Edc3	T	C	9: 57,647,197 (GRCm39)	V282A	probably benign	Het
Epha1	A	G	6: 42,339,602 (GRCm39)	V630A	probably damaging	Het
Erc1	A	G	6: 119,801,564 (GRCm39)	I151T	probably benign	Het
Fam83h	C	T	15: 75,875,699 (GRCm39)	R546H	probably damaging	Het
Fbxl14	A	T	6: 119,457,742 (GRCm39)	K308*	probably null	Het
Gldn	T	A	9: 54,245,976 (GRCm39)	M509K	probably benign	Het
Gpr139	T	C	7: 118,743,866 (GRCm39)	I240V	probably benign	Het
Herc1	G	T	9: 66,347,116 (GRCm39)	M1990I	possibly damaging	Het
Hmgcr	A	G	13: 96,793,231 (GRCm39)	I467T	probably benign	Het
Isl2	T	C	9: 55,449,685 (GRCm39)	F85L	probably benign	Het
Lat	T	C	7: 125,963,612 (GRCm39)	N197D	probably damaging	Het
Marchf1	T	C	8: 66,921,169 (GRCm39)	V282A	probably benign	Het
Meiosin	T	A	7: 18,835,044 (GRCm39)	D393V	unknown	Het
Mrpl9	A	G	3: 94,351,075 (GRCm39)	H85R	possibly damaging	Het
Mterf2	G	T	10: 84,956,381 (GRCm39)	A81E	probably damaging	Het
Mtr	A	G	13: 12,264,782 (GRCm39)	C107R	probably benign	Het
Nlrp4c	T	A	7: 6,075,635 (GRCm39)	V642E	probably damaging	Het
Or5b12b	A	G	19: 12,861,871 (GRCm39)	I209V	probably benign	Het
Or6c212	T	A	10: 129,559,018 (GRCm39)	I132F	probably benign	Het
Pdik1l	A	G	4: 134,006,804 (GRCm39)	S112P	unknown	Het
Pex19	GTCTCTTGTCTCCGAAGGTGCTCTTGATGATTTCTCTTGTCTCCGAAGGTGCTCTTGATGATTTC	GTCTCTTGTCTCCGAAGGTGCTCTTGATGATTTC	1: 171,956,150 (GRCm39)		probably null	Het
Rest	T	A	5: 77,416,119 (GRCm39)	L111Q	probably damaging	Het
Slc28a2b	A	C	2: 122,352,110 (GRCm39)		probably null	Het
Spg7	A	C	8: 123,800,617 (GRCm39)	D169A	possibly damaging	Het
Syt3	T	C	7: 44,042,083 (GRCm39)	V314A	possibly damaging	Het
Tbc1d20	G	C	2: 152,150,004 (GRCm39)	G144R	probably damaging	Het
Tlk1	A	T	2: 70,617,016 (GRCm39)		probably null	Het
Tmem147	T	G	7: 30,427,716 (GRCm39)		probably null	Het
Trip12	A	T	1: 84,755,170 (GRCm39)	D401E	probably damaging	Het
Ulbp1	A	G	10: 7,395,685 (GRCm39)	S291P	unknown	Het
Usp19	A	G	9: 108,379,112 (GRCm39)	D1286G	possibly damaging	Het
Usp49	T	C	17: 47,989,873 (GRCm39)	F553L	probably damaging	Het
Zfp352	A	G	4: 90,113,512 (GRCm39)	T551A	possibly damaging	Het

Other mutations in Fbxw24

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01093:Fbxw24	APN	9	109,434,041 (GRCm39)	missense	probably benign	0.00
IGL01373:Fbxw24	APN	9	109,452,701 (GRCm39)	missense	probably damaging	0.98
IGL01996:Fbxw24	APN	9	109,434,440 (GRCm39)	missense	possibly damaging	0.84
IGL02179:Fbxw24	APN	9	109,438,973 (GRCm39)	nonsense	probably null
IGL02718:Fbxw24	APN	9	109,453,858 (GRCm39)	missense	possibly damaging	0.55
IGL02936:Fbxw24	APN	9	109,454,026 (GRCm39)	splice site	probably null
IGL03010:Fbxw24	APN	9	109,452,678 (GRCm39)	missense	probably benign	0.07
IGL03350:Fbxw24	APN	9	109,436,081 (GRCm39)	missense	probably damaging	1.00
IGL03402:Fbxw24	APN	9	109,430,316 (GRCm39)	missense	probably damaging	1.00
R0140:Fbxw24	UTSW	9	109,434,482 (GRCm39)	missense	possibly damaging	0.73
R0718:Fbxw24	UTSW	9	109,452,577 (GRCm39)	splice site	probably benign
R1166:Fbxw24	UTSW	9	109,436,066 (GRCm39)	missense	probably benign	0.00
R1550:Fbxw24	UTSW	9	109,436,112 (GRCm39)	missense	probably benign	0.00
R1950:Fbxw24	UTSW	9	109,434,481 (GRCm39)	missense	probably benign	0.02
R1986:Fbxw24	UTSW	9	109,436,124 (GRCm39)	missense	probably damaging	0.97
R2244:Fbxw24	UTSW	9	109,434,117 (GRCm39)	missense	possibly damaging	0.66
R3683:Fbxw24	UTSW	9	109,437,110 (GRCm39)	missense	possibly damaging	0.51
R4324:Fbxw24	UTSW	9	109,434,013 (GRCm39)	critical splice donor site	probably null
R4387:Fbxw24	UTSW	9	109,439,053 (GRCm39)	missense	probably damaging	1.00
R4409:Fbxw24	UTSW	9	109,437,256 (GRCm39)	missense	probably damaging	1.00
R4410:Fbxw24	UTSW	9	109,437,256 (GRCm39)	missense	probably damaging	1.00
R4803:Fbxw24	UTSW	9	109,453,910 (GRCm39)	missense	probably benign	0.02
R5571:Fbxw24	UTSW	9	109,436,066 (GRCm39)	missense	probably benign	0.00
R6042:Fbxw24	UTSW	9	109,436,079 (GRCm39)	missense	probably benign	0.09
R6523:Fbxw24	UTSW	9	109,434,048 (GRCm39)	nonsense	probably null
R6799:Fbxw24	UTSW	9	109,453,998 (GRCm39)	missense	probably damaging	1.00
R7122:Fbxw24	UTSW	9	109,430,328 (GRCm39)	missense	probably benign	0.03
R7239:Fbxw24	UTSW	9	109,434,598 (GRCm39)	missense	possibly damaging	0.71
R7405:Fbxw24	UTSW	9	109,436,136 (GRCm39)	missense	possibly damaging	0.46
R8536:Fbxw24	UTSW	9	109,452,599 (GRCm39)	missense	probably damaging	1.00
R8873:Fbxw24	UTSW	9	109,453,996 (GRCm39)	missense	probably damaging	1.00
R9332:Fbxw24	UTSW	9	109,452,681 (GRCm39)	missense	probably damaging	1.00
R9430:Fbxw24	UTSW	9	109,439,038 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CCACTGTGTCTTCAAGTCCAG -3'
(R):5'- GGGCTTGGAAAATCTTTGCATATAC -3'

Sequencing Primer
(F):5'- GTGTCTTCAAGTCCAGTAATGAGACC -3'
(R):5'- AAATACTATTGGAGGCCCTGGGG -3'

Posted On

2020-03-09