Incidental Mutation 'R0716:Gm5444'
ID 62855
Institutional Source Beutler Lab
Gene Symbol Gm5444
Ensembl Gene ENSMUSG00000053499
Gene Name predicted gene 5444
Synonyms
Accession Numbers
Essential gene? Probably non essential (E-score: 0.077) question?
Stock # R0716 (G1)
Quality Score 99
Status Not validated
Chromosome 13
Chromosomal Location 4821650-4886484 bp(+) (GRCm39)
Type of Mutation exon
DNA Base Change (assembly) A to G at 4884192 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change
Gene Model predicted gene model for transcript(s):
AlphaFold no structure available at present
Predicted Effect noncoding transcript
Transcript: ENSMUST00000065956
SMART Domains Protein: ENSMUSP00000070493
Gene: ENSMUSG00000053499

DomainStartEndE-ValueType
transmembrane domain 7 29 N/A INTRINSIC
low complexity region 83 103 N/A INTRINSIC
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.7%
  • 10x: 97.3%
  • 20x: 94.8%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 5 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Bmal2 A G 6: 146,731,218 (GRCm39) S508G possibly damaging Het
Pkd1l2 A T 8: 117,777,839 (GRCm39) V904E probably damaging Het
Prl3b1 A G 13: 27,427,779 (GRCm39) T30A probably benign Het
Ssb A G 2: 69,697,703 (GRCm39) T145A probably benign Het
Vmn2r110 T C 17: 20,794,165 (GRCm39) R835G probably damaging Het
Other mutations in Gm5444
AlleleSourceChrCoordTypePredicted EffectPPH Score
R2332:Gm5444 UTSW 13 4,883,624 (GRCm39) exon noncoding transcript
R2341:Gm5444 UTSW 13 4,884,344 (GRCm39) exon noncoding transcript
R3901:Gm5444 UTSW 13 4,884,278 (GRCm39) exon noncoding transcript
R4520:Gm5444 UTSW 13 4,884,225 (GRCm39) exon noncoding transcript
R5148:Gm5444 UTSW 13 4,884,314 (GRCm39) exon noncoding transcript
R5856:Gm5444 UTSW 13 4,821,683 (GRCm39) exon noncoding transcript
Predicted Primers PCR Primer
(F):5'- GCTCCAGCCCCACTAGCTTCAG -3'
(R):5'- GAGCCTTTGTCATTGCTTCCAGATTTAC -3'

Sequencing Primer
(F):5'- ccaccatgcttggatttgaac -3'
(R):5'- gccagaaagcagagatgtgaag -3'
Posted On 2013-07-30