Mutagenetix > Incidental Mutation

Incidental Mutation 'R7848:Gstm7'

628675

Institutional Source

Beutler Lab

Gene Symbol

Gstm7

Ensembl Gene

ENSMUSG00000004035

Gene Name

glutathione S-transferase, mu 7

Synonyms

Cd203c, 0610005A07Rik

MMRRC Submission

045902-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.194) question?

Stock #

R7848 (G1)

Quality Score

103.008

Status

Validated

Chromosome

Chromosomal Location

107833650-107839133 bp(-) (GRCm39)

Type of Mutation

splice site (284 bp from exon)

DNA Base Change (assembly)

A to T at 107835902 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000102299 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000004137] [ENSMUST00000106687] [ENSMUST00000106688] [ENSMUST00000124215] [ENSMUST00000133947]

AlphaFold

Q80W21

Predicted Effect

probably null
Transcript: ENSMUST00000004137

SMART Domains

Protein: ENSMUSP00000004137
Gene: ENSMUSG00000004035

Domain	Start	End	E-Value	Type
Pfam:GST_N	3	82	2.2e-23	PFAM
Pfam:GST_C_3	42	190	1.2e-9	PFAM
Pfam:GST_C	104	191	5.3e-15	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000106687

SMART Domains

Protein: ENSMUSP00000102298
Gene: ENSMUSG00000004035

Domain	Start	End	E-Value	Type
Pfam:GST_N	3	82	6.8e-24	PFAM
Pfam:GST_N_3	11	93	1.1e-6	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000106688

SMART Domains

Protein: ENSMUSP00000102299
Gene: ENSMUSG00000004035

Domain	Start	End	E-Value	Type
Pfam:GST_N_3	4	89	8.8e-7	PFAM
Pfam:GST_N	5	78	4.2e-19	PFAM
Pfam:GST_C	100	188	5.6e-16	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000124215

SMART Domains

Protein: ENSMUSP00000118707
Gene: ENSMUSG00000004035

Domain	Start	End	E-Value	Type
Pfam:GST_N	1	72	8.6e-20	PFAM
Pfam:GST_N_3	3	83	4e-7	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000133947

SMART Domains

Protein: ENSMUSP00000122567
Gene: ENSMUSG00000004035

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:GST_N	45	123	2.6e-19	PFAM
Pfam:GST_N_3	54	134	1.4e-6	PFAM

Meta Mutation Damage Score

0.9756

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (62/62)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2900026A02Rik	C	T	5: 113,340,007 (GRCm39)	A2T	probably damaging	Het
Abca3	G	T	17: 24,603,506 (GRCm39)	G566V	probably damaging	Het
Aff4	C	A	11: 53,295,339 (GRCm39)	N846K	probably benign	Het
Aldh1l2	C	T	10: 83,335,707 (GRCm39)	R714Q	probably benign	Het
Cbln1	T	C	8: 88,198,328 (GRCm39)	T126A	probably damaging	Het
Ccdc85a	A	G	11: 28,346,123 (GRCm39)	S446P	possibly damaging	Het
Ccdc87	A	G	19: 4,891,536 (GRCm39)	Q676R	probably damaging	Het
Cenatac	A	G	9: 44,324,939 (GRCm39)	S139P	probably damaging	Het
Col26a1	T	C	5: 136,775,907 (GRCm39)	K349E	possibly damaging	Het
Col6a5	T	C	9: 105,805,385 (GRCm39)	I1174V	unknown	Het
Cyth1	TGGGCAA	T	11: 118,074,749 (GRCm39)		probably null	Het
Dmxl1	A	G	18: 49,973,557 (GRCm39)	D64G	possibly damaging	Het
Dnajc15	T	C	14: 78,077,643 (GRCm39)	H114R	probably damaging	Het
Espl1	T	C	15: 102,224,961 (GRCm39)	F1390S	probably damaging	Het
F5	T	C	1: 163,989,446 (GRCm39)	I116T	possibly damaging	Het
Fam120b	T	C	17: 15,626,036 (GRCm39)	V463A	possibly damaging	Het
Fat4	A	G	3: 38,942,000 (GRCm39)	M298V	probably benign	Het
Fhad1	A	T	4: 141,632,913 (GRCm39)	M1197K	probably benign	Het
Fn1	T	A	1: 71,689,760 (GRCm39)	I127F	probably damaging	Het
Frmd4a	A	G	2: 4,596,728 (GRCm39)		probably benign	Het
Gabpb2	A	T	3: 95,097,959 (GRCm39)	V238E	probably damaging	Het
Gnpat	C	A	8: 125,613,630 (GRCm39)	Q626K	possibly damaging	Het
Gys2	G	T	6: 142,391,741 (GRCm39)	S507*	probably null	Het
Ippk	T	A	13: 49,596,972 (GRCm39)		probably null	Het
Itga8	T	A	2: 12,196,548 (GRCm39)	N623I	probably damaging	Het
Kcnb1	A	G	2: 166,948,188 (GRCm39)	F220S	probably damaging	Het
Kiz	T	A	2: 146,731,100 (GRCm39)	S197T	probably benign	Het
Klhl42	A	G	6: 147,009,598 (GRCm39)	N479S	probably damaging	Het
Lims2	A	G	18: 32,091,301 (GRCm39)	*60W	probably null	Het
Lrch4	A	G	5: 137,632,116 (GRCm39)	N124S	probably damaging	Het
Man2a2	G	C	7: 80,018,613 (GRCm39)	A82G	probably benign	Het
Map3k13	T	C	16: 21,724,621 (GRCm39)	V373A	probably damaging	Het
Mapkapk5	A	G	5: 121,683,232 (GRCm39)	I11T	probably benign	Het
Mroh2b	C	T	15: 4,967,861 (GRCm39)	Q967*	probably null	Het
Mthfd1l	A	G	10: 4,033,739 (GRCm39)	T709A	possibly damaging	Het
Muc6	T	C	7: 141,232,188 (GRCm39)	T939A	possibly damaging	Het
Ncam2	C	T	16: 81,287,267 (GRCm39)	H394Y	probably benign	Het
Ncoa7	A	T	10: 30,524,414 (GRCm39)	N161K	possibly damaging	Het
Nr2c1	C	T	10: 94,026,508 (GRCm39)	S461L	probably benign	Het
Nrg3	T	C	14: 38,390,240 (GRCm39)	E323G	probably damaging	Het
Nufip1	G	A	14: 76,351,661 (GRCm39)	R172H	probably damaging	Het
Nup210l	A	G	3: 90,111,212 (GRCm39)	T1705A	probably benign	Het
Oaf	G	A	9: 43,134,077 (GRCm39)	R215C	probably damaging	Het
Ogfr	T	C	2: 180,234,226 (GRCm39)	L99P	probably damaging	Het
Or10am5	T	C	7: 6,517,861 (GRCm39)	D189G	probably damaging	Het
Or51a43	T	A	7: 103,717,302 (GRCm39)	N312I	probably benign	Het
Pcare	T	C	17: 72,056,188 (GRCm39)	D1163G	probably benign	Het
Pcdh18	A	T	3: 49,710,446 (GRCm39)	S290T	possibly damaging	Het
Pklr	T	G	3: 89,050,285 (GRCm39)	I378S	possibly damaging	Het
Rbm12	A	T	2: 155,938,136 (GRCm39)	M712K	probably benign	Het
Rnase10	T	C	14: 51,246,970 (GRCm39)	V116A	possibly damaging	Het
Scube3	T	C	17: 28,384,569 (GRCm39)	L621P	probably benign	Het
Slc35e1	T	C	8: 73,246,280 (GRCm39)	I51V	probably benign	Het
Smcr8	A	G	11: 60,670,750 (GRCm39)	T633A	probably benign	Het
St18	T	C	1: 6,927,669 (GRCm39)		probably null	Het
Tm9sf4	T	A	2: 153,044,275 (GRCm39)	I509N	probably damaging	Het
Tmcc2	T	C	1: 132,288,359 (GRCm39)	K443E	probably damaging	Het
Tmem98	T	G	11: 80,710,758 (GRCm39)	V139G	probably damaging	Het
Trgv5	G	A	13: 19,376,849 (GRCm39)	V99I	probably damaging	Het
Ttll1	T	C	15: 83,381,573 (GRCm39)	E232G	probably damaging	Het
Zfp442	T	C	2: 150,253,146 (GRCm39)	N39D	possibly damaging	Het

Other mutations in Gstm7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02215:Gstm7	APN	3	107,837,594 (GRCm39)	missense	possibly damaging	0.93
PIT4142001:Gstm7	UTSW	3	107,838,799 (GRCm39)	frame shift	probably null
R0095:Gstm7	UTSW	3	107,837,879 (GRCm39)	splice site	probably benign
R0961:Gstm7	UTSW	3	107,834,302 (GRCm39)	unclassified	probably benign
R1052:Gstm7	UTSW	3	107,834,266 (GRCm39)	missense	probably benign	0.05
R2121:Gstm7	UTSW	3	107,834,230 (GRCm39)	missense	probably benign	0.00
R4610:Gstm7	UTSW	3	107,834,235 (GRCm39)	missense	possibly damaging	0.65
R5966:Gstm7	UTSW	3	107,838,747 (GRCm39)	intron	probably benign
R6393:Gstm7	UTSW	3	107,838,142 (GRCm39)	critical splice donor site	probably null
R7014:Gstm7	UTSW	3	107,834,278 (GRCm39)	missense	probably benign	0.00
R7052:Gstm7	UTSW	3	107,838,633 (GRCm39)	missense	probably damaging	0.96
R7741:Gstm7	UTSW	3	107,838,963 (GRCm39)	missense	possibly damaging	0.90
R7988:Gstm7	UTSW	3	107,834,271 (GRCm39)	missense	possibly damaging	0.82
R7998:Gstm7	UTSW	3	107,837,657 (GRCm39)	missense	probably damaging	1.00
R8952:Gstm7	UTSW	3	107,838,757 (GRCm39)	intron	probably benign

Predicted Primers

PCR Primer
(F):5'- TGGTATTGTCTACACGCGGC -3'
(R):5'- GCTTAAAATCAGCAAAAGCCAGTAG -3'

Sequencing Primer
(F):5'- TACACGCGGCTATTTCACAC -3'
(R):5'- AGCATGTACTGAGTGCTTGC -3'

Posted On

2020-06-09