Mutagenetix > Incidental Mutation

Incidental Mutation 'R8075:Zfp867'

628976

Institutional Source

Beutler Lab

Gene Symbol

Zfp867

Ensembl Gene

ENSMUSG00000054519

Gene Name

zinc finger protein 867

Synonyms

MMRRC Submission

067509-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.438) question?

Stock #

R8075 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

59352023-59363300 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 59355066 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 88 (S88P)

Ref Sequence

ENSEMBL: ENSMUSP00000050746 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000057799]

AlphaFold

E9Q2M4

Predicted Effect

probably benign
Transcript: ENSMUST00000057799
AA Change: S88P

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000050746
Gene: ENSMUSG00000054519
AA Change: S88P

Domain	Start	End	E-Value	Type
KRAB	12	70	1.36e-16	SMART
low complexity region	84	90	N/A	INTRINSIC
ZnF_C2H2	121	143	4.47e-3	SMART
ZnF_C2H2	149	171	5.59e-4	SMART
ZnF_C2H2	177	199	5.5e-3	SMART
ZnF_C2H2	205	227	5.14e-3	SMART
ZnF_C2H2	233	255	1.2e-3	SMART
ZnF_C2H2	261	283	5.5e-3	SMART
ZnF_C2H2	289	311	1.38e-3	SMART
ZnF_C2H2	317	339	5.42e-2	SMART
ZnF_C2H2	346	368	1.47e-3	SMART
ZnF_C2H2	374	396	7.11e0	SMART
ZnF_C2H2	402	424	1.69e-3	SMART

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 98.8%
20x: 95.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acad10	A	T	5: 121,790,148 (GRCm39)	H69Q	probably benign	Het
Aif1	T	A	17: 35,390,811 (GRCm39)	N87Y	unknown	Het
Caprin2	A	T	6: 148,770,590 (GRCm39)	V468E	probably benign	Het
Cep68	A	T	11: 20,189,335 (GRCm39)	V559D	probably benign	Het
Chrna4	A	C	2: 180,680,859 (GRCm39)	I3S	unknown	Het
Cog3	T	C	14: 75,968,142 (GRCm39)	Y407C	probably damaging	Het
Col15a1	A	G	4: 47,208,359 (GRCm39)	H3R	probably benign	Het
Ctxn1	A	G	8: 4,308,553 (GRCm39)	V26A	probably benign	Het
Cyp27b1	A	C	10: 126,887,382 (GRCm39)	T405P	probably damaging	Het
Cyp2c65	A	G	19: 39,060,682 (GRCm39)	I181V	probably benign	Het
Dusp6	T	A	10: 99,100,810 (GRCm39)	S269T	possibly damaging	Het
Efcab6	A	T	15: 83,851,824 (GRCm39)	D351E	probably damaging	Het
Fam181a	A	T	12: 103,282,296 (GRCm39)	H67L	possibly damaging	Het
Fbxl18	A	G	5: 142,871,861 (GRCm39)	L458P	probably damaging	Het
Flnb	T	C	14: 7,913,048 (GRCm38)	I1439T	probably benign	Het
Foxj2	C	T	6: 122,815,055 (GRCm39)	Q364*	probably null	Het
Gm3138	A	T	14: 4,250,532 (GRCm38)	N55Y	probably damaging	Het
Hmcn2	A	G	2: 31,279,403 (GRCm39)	T1802A	possibly damaging	Het
Hoxc6	T	C	15: 102,919,325 (GRCm39)	I187T	probably damaging	Het
Ighg3	T	A	12: 113,321,097 (GRCm39)	I387F		Het
Insr	A	T	8: 3,205,862 (GRCm39)	M1309K	probably benign	Het
Itsn1	A	T	16: 91,686,097 (GRCm39)	N1290I	unknown	Het
Jag2	C	A	12: 112,878,894 (GRCm39)	R509L	probably benign	Het
Kctd11	T	A	11: 69,771,095 (GRCm39)		probably benign	Het
Med13	C	A	11: 86,163,296 (GRCm39)	V2126F	probably damaging	Het
Mknk2	G	A	10: 80,507,982 (GRCm39)		probably benign	Het
Oga	A	T	19: 45,749,621 (GRCm39)	N699K	probably damaging	Het
Or52l1	T	G	7: 104,830,343 (GRCm39)	H74P	probably damaging	Het
Or5m13b	A	G	2: 85,754,470 (GRCm39)	N286S	probably benign	Het
Pdzrn4	T	C	15: 92,575,605 (GRCm39)	V337A	probably damaging	Het
Pik3c3	A	G	18: 30,438,082 (GRCm39)	N491D	probably damaging	Het
Pla2g12b	T	A	10: 59,257,274 (GRCm39)	S96T	unknown	Het
Rab37	G	T	11: 114,982,759 (GRCm39)		probably null	Het
Rp9	G	A	9: 22,368,788 (GRCm39)	T57M	probably damaging	Het
Rrp12	A	G	19: 41,851,713 (GRCm39)	V1274A	probably damaging	Het
Serpinb11	G	A	1: 107,298,519 (GRCm39)	V57M	probably damaging	Het
Slc1a7	G	A	4: 107,869,473 (GRCm39)	V513M	probably benign	Het
Slc39a14	A	T	14: 70,546,247 (GRCm39)	I392N	possibly damaging	Het
Sowahb	G	A	5: 93,192,276 (GRCm39)	Q148*	probably null	Het
Spon1	T	C	7: 113,616,026 (GRCm39)		probably null	Het
Susd4	C	A	1: 182,592,748 (GRCm39)	T48K	possibly damaging	Het
Taf4b	T	C	18: 14,916,749 (GRCm39)	V33A	possibly damaging	Het
Tas2r139	T	A	6: 42,118,154 (GRCm39)	N95K	probably benign	Het
Tle3	T	C	9: 61,281,841 (GRCm39)	M57T	probably benign	Het
Usp13	T	A	3: 32,985,852 (GRCm39)	M815K	probably damaging	Het
Vps8	A	G	16: 21,340,644 (GRCm39)	D796G	probably damaging	Het
Wnk1	C	T	6: 119,909,675 (GRCm39)	G41S	probably damaging	Het

Other mutations in Zfp867

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03156:Zfp867	APN	11	59,355,834 (GRCm39)	splice site	probably benign
IGL03338:Zfp867	APN	11	59,355,003 (GRCm39)	nonsense	probably null
R0040:Zfp867	UTSW	11	59,354,691 (GRCm39)	missense	possibly damaging	0.88
R1997:Zfp867	UTSW	11	59,354,417 (GRCm39)	missense	probably damaging	0.98
R2251:Zfp867	UTSW	11	59,356,319 (GRCm39)	nonsense	probably null
R4015:Zfp867	UTSW	11	59,354,520 (GRCm39)	missense	probably damaging	1.00
R4429:Zfp867	UTSW	11	59,355,863 (GRCm39)	missense	possibly damaging	0.55
R4697:Zfp867	UTSW	11	59,354,487 (GRCm39)	missense	probably damaging	1.00
R7428:Zfp867	UTSW	11	59,354,760 (GRCm39)	missense	probably benign	0.03
R7736:Zfp867	UTSW	11	59,354,016 (GRCm39)	missense	probably damaging	1.00
R8774:Zfp867	UTSW	11	59,354,837 (GRCm39)	missense	probably damaging	0.96
R8774-TAIL:Zfp867	UTSW	11	59,354,837 (GRCm39)	missense	probably damaging	0.96
R9674:Zfp867	UTSW	11	59,355,850 (GRCm39)	missense	probably benign	0.06

Predicted Primers

PCR Primer
(F):5'- CCCCAGTGTGAGTTCTTAAATGG -3'
(R):5'- AGTAAACCAACCTTCACTATGTGC -3'

Sequencing Primer
(F):5'- AATGGATTCTGAACTCACTGGG -3'
(R):5'- GTGCTTACCTTTTGTACAGAAGTC -3'

Posted On

2020-06-30