Mutagenetix > Incidental Mutation

Incidental Mutation 'R8076:Or10ag2'

629003

Institutional Source

Beutler Lab

Gene Symbol

Or10ag2

Ensembl Gene

ENSMUSG00000043274

Gene Name

olfactory receptor family 10 subfamily AG member 2

Synonyms

Olfr1123, MOR264-17, GA_x6K02T2Q125-48917235-48918206

MMRRC Submission

067510-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.281) question?

Stock #

R8076 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

87248394-87249365 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 87248889 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Leucine at position 164 (I164L)

Ref Sequence

ENSEMBL: ENSMUSP00000154459 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000054974] [ENSMUST00000216208]

AlphaFold

A2AT85

Predicted Effect

probably benign
Transcript: ENSMUST00000054974
AA Change: I166L

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000058786
Gene: ENSMUSG00000043274
AA Change: I166L

Domain	Start	End	E-Value	Type
Pfam:7tm_4	44	321	4.4e-52	PFAM
Pfam:7tm_1	54	303	2.6e-21	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000216208
AA Change: I164L

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.8%
20x: 95.1%

Validation Efficiency

98% (62/63)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam9	T	A	8: 25,452,938 (GRCm39)	R790*	probably null	Het
Adamts13	C	A	2: 26,880,624 (GRCm39)	P736T	probably benign	Het
Aldh3b2	T	A	19: 4,028,859 (GRCm39)	F176L	possibly damaging	Het
Aldoart2	A	G	12: 55,612,696 (GRCm39)	E207G	probably benign	Het
Arid4b	A	T	13: 14,361,535 (GRCm39)	E753D	probably benign	Het
Cd160	T	A	3: 96,709,662 (GRCm39)	T158S	probably benign	Het
Cd209g	T	A	8: 4,185,195 (GRCm39)	M1K	probably null	Het
Chrm4	A	G	2: 91,758,204 (GRCm39)	Y204C	probably damaging	Het
Cyld	T	A	8: 89,456,346 (GRCm39)	N465K	probably benign	Het
Dchs1	A	T	7: 105,405,128 (GRCm39)	H2471Q	possibly damaging	Het
Dchs1	A	G	7: 105,411,189 (GRCm39)	V1612A	probably damaging	Het
Ddx24	A	G	12: 103,382,477 (GRCm39)	V702A	probably damaging	Het
Dnah8	A	G	17: 31,003,127 (GRCm39)	T3609A	possibly damaging	Het
Dock5	A	T	14: 68,040,426 (GRCm39)		probably null	Het
Dpy19l2	C	T	9: 24,591,988 (GRCm39)	R205H	probably damaging	Het
Eif3a	A	T	19: 60,762,363 (GRCm39)	D473E	probably damaging	Het
Elapor1	G	T	3: 108,399,398 (GRCm39)	T64K	probably benign	Het
Elobl	T	C	11: 88,855,796 (GRCm39)	E89G	possibly damaging	Het
Fam186a	A	T	15: 99,841,351 (GRCm39)	I1631N	possibly damaging	Het
Fbn2	C	T	18: 58,159,496 (GRCm39)	W2411*	probably null	Het
Fbxl17	A	G	17: 63,367,360 (GRCm39)	I671T	probably damaging	Het
Glul	C	T	1: 153,782,868 (GRCm39)	T193I	possibly damaging	Het
Grm7	A	G	6: 111,543,000 (GRCm39)	Y907C	probably damaging	Het
H4c16	A	G	6: 136,781,124 (GRCm39)	M85T	probably benign	Het
Hr	A	G	14: 70,795,381 (GRCm39)	T309A	probably benign	Het
Ighg3	A	G	12: 113,324,158 (GRCm39)	S110P	probably benign	Het
Llgl2	A	G	11: 115,737,755 (GRCm39)	E180G	possibly damaging	Het
Mat2b	A	T	11: 40,576,092 (GRCm39)	L112Q	probably damaging	Het
Mcoln2	T	C	3: 145,896,169 (GRCm39)	M497T	probably damaging	Het
Mmp24	C	A	2: 155,649,481 (GRCm39)	Y299*	probably null	Het
Mtor	T	C	4: 148,610,260 (GRCm39)		probably null	Het
Olig2	C	A	16: 91,023,299 (GRCm39)	D4E	probably damaging	Het
Pard3	T	C	8: 128,142,077 (GRCm39)	S878P	probably damaging	Het
Pcgf5	C	T	19: 36,417,483 (GRCm39)	P137L	probably damaging	Het
Pck1	A	G	2: 172,997,071 (GRCm39)	N238D	probably damaging	Het
Pstpip1	T	C	9: 56,035,064 (GRCm39)	S346P	probably benign	Het
Qsox2	G	A	2: 26,114,897 (GRCm39)	H40Y	possibly damaging	Het
Rbpj	T	C	5: 53,799,479 (GRCm39)	I129T	probably damaging	Het
Rsf1	ATGGCG	ATGGCGACGGTGGCG	7: 97,229,111 (GRCm39)		probably benign	Het
Saal1	G	A	7: 46,360,031 (GRCm39)	P9L	probably benign	Het
Sema3c	A	G	5: 17,932,362 (GRCm39)	I622V	probably benign	Het
Sigirr	C	T	7: 140,671,785 (GRCm39)	V333M	probably benign	Het
Sort1	T	C	3: 108,246,183 (GRCm39)	S387P	probably damaging	Het
Spata31h1	A	C	10: 82,132,520 (GRCm39)	Y163*	probably null	Het
Spta1	T	A	1: 174,014,797 (GRCm39)	S426T	probably benign	Het
Stag3	G	A	5: 138,281,404 (GRCm39)	S124N	probably damaging	Het
Stard10	A	G	7: 100,993,176 (GRCm39)	Y244C	probably damaging	Het
Tcea3	T	A	4: 135,995,440 (GRCm39)	I261N	probably damaging	Het
Tdrd1	A	G	19: 56,832,267 (GRCm39)	K395E	probably damaging	Het
Tenm2	T	A	11: 35,918,048 (GRCm39)	D1905V	probably benign	Het
Trim12c	T	C	7: 103,990,037 (GRCm39)	Y480C	unknown	Het
Trip11	A	G	12: 101,849,741 (GRCm39)	V1441A	probably damaging	Het
Ttc21a	G	A	9: 119,795,392 (GRCm39)	R1107Q	probably benign	Het
Wdr41	A	G	13: 95,153,838 (GRCm39)	I296V	probably benign	Het
Zdbf2	T	A	1: 63,345,260 (GRCm39)	V1213E	possibly damaging	Het
Zfp37	T	C	4: 62,109,553 (GRCm39)	N545D	possibly damaging	Het

Other mutations in Or10ag2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00231:Or10ag2	APN	2	87,248,910 (GRCm39)	missense	possibly damaging	0.75
IGL01067:Or10ag2	APN	2	87,248,714 (GRCm39)	missense	probably benign	0.00
IGL01526:Or10ag2	APN	2	87,249,319 (GRCm39)	missense	probably damaging	0.99
IGL01580:Or10ag2	APN	2	87,248,880 (GRCm39)	missense	probably benign	0.06
IGL01986:Or10ag2	APN	2	87,248,880 (GRCm39)	missense	probably benign	0.00
IGL02503:Or10ag2	APN	2	87,248,636 (GRCm39)	missense	probably benign	0.03
IGL02527:Or10ag2	APN	2	87,249,181 (GRCm39)	missense	probably damaging	1.00
IGL02731:Or10ag2	APN	2	87,249,051 (GRCm39)	missense	probably benign	0.00
IGL03215:Or10ag2	APN	2	87,248,412 (GRCm39)	missense	probably benign	0.05
IGL03366:Or10ag2	APN	2	87,248,587 (GRCm39)	missense	possibly damaging	0.88
R0645:Or10ag2	UTSW	2	87,248,612 (GRCm39)	nonsense	probably null
R1857:Or10ag2	UTSW	2	87,248,992 (GRCm39)	missense	probably damaging	1.00
R2175:Or10ag2	UTSW	2	87,248,500 (GRCm39)	missense	probably damaging	1.00
R3691:Or10ag2	UTSW	2	87,248,514 (GRCm39)	missense	probably benign	0.20
R4082:Or10ag2	UTSW	2	87,248,801 (GRCm39)	nonsense	probably null
R4635:Or10ag2	UTSW	2	87,249,043 (GRCm39)	missense	probably benign	0.05
R4877:Or10ag2	UTSW	2	87,248,907 (GRCm39)	nonsense	probably null
R5190:Or10ag2	UTSW	2	87,249,187 (GRCm39)	missense	probably damaging	1.00
R5253:Or10ag2	UTSW	2	87,249,012 (GRCm39)	missense	possibly damaging	0.64
R6266:Or10ag2	UTSW	2	87,249,350 (GRCm39)	missense	probably benign	0.01
R6784:Or10ag2	UTSW	2	87,248,796 (GRCm39)	missense	probably benign	0.20
R6909:Or10ag2	UTSW	2	87,248,959 (GRCm39)	missense	probably damaging	1.00
R7255:Or10ag2	UTSW	2	87,249,286 (GRCm39)	missense	probably damaging	0.96
R8116:Or10ag2	UTSW	2	87,249,081 (GRCm39)	missense	probably damaging	1.00
R8315:Or10ag2	UTSW	2	87,248,995 (GRCm39)	missense	probably damaging	1.00
R8888:Or10ag2	UTSW	2	87,248,659 (GRCm39)	missense	probably benign	0.00
R8895:Or10ag2	UTSW	2	87,248,659 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GCCTGTGCCACTCAAATGTG -3'
(R):5'- GCTGATGACAGTTTCAGAATACTG -3'

Sequencing Primer
(F):5'- GTGCCACTCAAATGTGTTTTATCC -3'
(R):5'- TTTGCAATAGGAGAAAATGATCAGC -3'

Posted On

2020-06-30