Mutagenetix > Incidental Mutation

Incidental Mutation 'R8081:Lats2'

629376

Institutional Source

Beutler Lab

Gene Symbol

Lats2

Ensembl Gene

ENSMUSG00000021959

Gene Name

large tumor suppressor 2

Synonyms

MMRRC Submission

067514-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R8081 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

57927119-57983669 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 57937968 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Cysteine at position 174 (G174C)

Ref Sequence

ENSEMBL: ENSMUSP00000022531 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022531] [ENSMUST00000173964] [ENSMUST00000173990] [ENSMUST00000174166] [ENSMUST00000174213] [ENSMUST00000174694]

AlphaFold

Q7TSJ6

PDB Structure

Solution structure of RSGI RUH-038, a UBA domain from Mouse LATS2 (Large Tumor Suppressor homolog 2) [SOLUTION NMR]

Predicted Effect

probably damaging
Transcript: ENSMUST00000022531
AA Change: G174C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000022531
Gene: ENSMUSG00000021959
AA Change: G174C

Domain	Start	End	E-Value	Type
PDB:2COS\|A	91	138	3e-20	PDB
low complexity region	210	223	N/A	INTRINSIC
low complexity region	401	408	N/A	INTRINSIC
low complexity region	437	444	N/A	INTRINSIC
low complexity region	471	482	N/A	INTRINSIC
low complexity region	517	529	N/A	INTRINSIC
S_TKc	626	931	2.94e-94	SMART
S_TK_X	932	1002	1.21e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000173964

SMART Domains

Protein: ENSMUSP00000134142
Gene: ENSMUSG00000021959

Domain	Start	End	E-Value	Type
low complexity region	21	28	N/A	INTRINSIC
low complexity region	57	64	N/A	INTRINSIC
low complexity region	91	102	N/A	INTRINSIC
low complexity region	137	149	N/A	INTRINSIC
Pfam:Pkinase	233	288	2.3e-5	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000173990
AA Change: G174C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000133976
Gene: ENSMUSG00000021959
AA Change: G174C

Domain	Start	End	E-Value	Type
PDB:2COS\|A	91	138	8e-22	PDB
low complexity region	210	223	N/A	INTRINSIC
low complexity region	401	408	N/A	INTRINSIC
low complexity region	437	444	N/A	INTRINSIC
low complexity region	471	482	N/A	INTRINSIC
low complexity region	517	529	N/A	INTRINSIC
S_TKc	626	893	7.75e-71	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000174166

SMART Domains

Protein: ENSMUSP00000133379
Gene: ENSMUSG00000021959

Domain	Start	End	E-Value	Type
PDB:2COS\|A	91	114	5e-7	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000174213

SMART Domains

Protein: ENSMUSP00000134321
Gene: ENSMUSG00000021959

Domain	Start	End	E-Value	Type
PDB:2COS\|A	91	114	2e-6	PDB

Predicted Effect

probably damaging
Transcript: ENSMUST00000174694
AA Change: G174C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000133680
Gene: ENSMUSG00000114942
AA Change: G174C

Domain	Start	End	E-Value	Type
PDB:2COS\|A	91	138	7e-22	PDB
low complexity region	210	223	N/A	INTRINSIC
low complexity region	401	408	N/A	INTRINSIC
low complexity region	437	444	N/A	INTRINSIC
low complexity region	471	482	N/A	INTRINSIC
low complexity region	517	529	N/A	INTRINSIC
Pfam:Pkinase	626	792	2.2e-38	PFAM
Pfam:Pkinase_Tyr	626	795	2.8e-21	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.9%
20x: 95.7%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous null mice display embryonic lethality with decreased cell proliferation, chromosomal instability, atrial hyperplasia, ventricular hypoplasia, delayed embryonic development, an irregular kinked neural tube, and hemorrhages. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700003E16Rik	A	T	6: 83,138,313 (GRCm39)	E119V	probably damaging	Het
Adamts2	A	T	11: 50,668,004 (GRCm39)	D522V	probably damaging	Het
Agtpbp1	A	T	13: 59,676,221 (GRCm39)	L183*	probably null	Het
Arhgap10	T	C	8: 78,109,375 (GRCm39)	I403V	possibly damaging	Het
Arhgef11	T	C	3: 87,632,949 (GRCm39)	S687P	probably damaging	Het
Cdhr1	C	T	14: 36,815,967 (GRCm39)	V144I	probably benign	Het
Cdk20	A	G	13: 64,586,766 (GRCm39)	I339V	probably benign	Het
Ceacam13	A	G	7: 17,747,113 (GRCm39)	K189E	probably damaging	Het
Cep290	C	T	10: 100,394,038 (GRCm39)	Q2082*	probably null	Het
Cgrrf1	A	G	14: 47,091,468 (GRCm39)	T331A	probably benign	Het
Cntn4	G	A	6: 106,651,568 (GRCm39)	V706I	possibly damaging	Het
Csrnp2	T	C	15: 100,387,462 (GRCm39)	D2G	probably damaging	Het
Cux2	A	G	5: 122,007,519 (GRCm39)	I714T	probably benign	Het
Cx3cr1	C	T	9: 119,880,878 (GRCm39)	E175K	possibly damaging	Het
Dennd1c	G	A	17: 57,381,139 (GRCm39)	P163L	possibly damaging	Het
Dhtkd1	C	T	2: 5,928,919 (GRCm39)	E251K	probably damaging	Het
Epas1	C	T	17: 87,136,797 (GRCm39)	P787S	probably benign	Het
Epha2	T	C	4: 141,049,605 (GRCm39)	V737A	probably damaging	Het
Fam50b	G	A	13: 34,931,084 (GRCm39)	E187K	possibly damaging	Het
Fcsk	T	A	8: 111,615,783 (GRCm39)	R515S	probably benign	Het
Fscb	A	T	12: 64,518,802 (GRCm39)	M888K	unknown	Het
Gldc	ACGACC	AC	19: 30,135,987 (GRCm39)		probably null	Het
Gm14443	A	T	2: 175,012,238 (GRCm39)	C69*	probably null	Het
Gm19668	G	A	10: 77,634,420 (GRCm39)	A183V	unknown	Het
Gm49368	A	G	7: 127,726,280 (GRCm39)	R1231G	unknown	Het
Grin2c	T	C	11: 115,140,719 (GRCm39)	Y1133C	probably damaging	Het
H3c13	C	A	3: 96,176,309 (GRCm39)	Y100*	probably null	Het
Hydin	T	C	8: 111,092,101 (GRCm39)	S425P	possibly damaging	Het
Ifnl3	T	C	7: 28,223,682 (GRCm39)	S173P	probably damaging	Het
Igkv4-63	A	T	6: 69,355,018 (GRCm39)	S88T	possibly damaging	Het
Klhl5	C	A	5: 65,320,268 (GRCm39)	N607K	possibly damaging	Het
Lag3	A	T	6: 124,882,410 (GRCm39)	L362*	probably null	Het
Mcm6	T	C	1: 128,265,905 (GRCm39)	E622G	probably damaging	Het
Med13l	C	A	5: 118,866,333 (GRCm39)	H462Q	probably damaging	Het
Mrpl53	T	A	6: 83,086,159 (GRCm39)	F23I	probably damaging	Het
Muc5b	T	G	7: 141,417,743 (GRCm39)	I3563S	possibly damaging	Het
Myo16	A	C	8: 10,372,743 (GRCm39)	L147F	unknown	Het
Npc1l1	G	C	11: 6,167,768 (GRCm39)	Q1008E	probably benign	Het
Npy4r	A	G	14: 33,868,524 (GRCm39)	S255P	probably damaging	Het
Nup210	T	C	6: 91,053,657 (GRCm39)	N287D	probably benign	Het
Opn3	T	A	1: 175,493,135 (GRCm39)	H143L	probably damaging	Het
Or12e7	G	T	2: 87,287,513 (GRCm39)	M1I	probably null	Het
Or12j3	A	T	7: 139,952,972 (GRCm39)	L184M	probably damaging	Het
Or12j5	A	T	7: 140,084,369 (GRCm39)	M1K	probably null	Het
Or4k2	T	A	14: 50,423,825 (GRCm39)	N284I	probably damaging	Het
Or5ap2b-ps1	C	T	2: 85,693,839 (GRCm39)	P28L	probably damaging	Het
Pdcd7	C	T	9: 65,253,967 (GRCm39)	R182C	probably damaging	Het
Peg10	GC	GCTCC	6: 4,756,452 (GRCm39)		probably benign	Het
Picalm	T	A	7: 89,840,451 (GRCm39)	L540*	probably null	Het
Ppp1r21	T	A	17: 88,866,272 (GRCm39)	I356N	probably damaging	Het
Rgs6	C	T	12: 83,094,347 (GRCm39)	Q67*	probably null	Het
Ripor2	C	A	13: 24,897,683 (GRCm39)	Q794K	probably benign	Het
Rnf216	A	T	5: 143,013,719 (GRCm39)	I702N	probably damaging	Het
Robo4	T	C	9: 37,316,936 (GRCm39)	L417P	probably damaging	Het
Samhd1	A	T	2: 156,943,358 (GRCm39)	C605*	probably null	Het
Scaper	C	T	9: 55,823,330 (GRCm39)	G22D	unknown	Het
Senp5	T	C	16: 31,784,577 (GRCm39)	T692A	probably damaging	Het
Six6	G	T	12: 72,986,875 (GRCm39)	G16W	probably damaging	Het
Slc38a8	A	T	8: 120,212,269 (GRCm39)	M358K	possibly damaging	Het
Snx2	T	C	18: 53,349,459 (GRCm39)	F407L	probably benign	Het
Srrm2	T	A	17: 24,039,219 (GRCm39)	N1954K	probably damaging	Het
Syk	A	T	13: 52,792,195 (GRCm39)	M429L	probably benign	Het
Tln2	T	A	9: 67,264,029 (GRCm39)	N537Y	probably damaging	Het
Usp29	T	A	7: 6,966,629 (GRCm39)	M824K	probably benign	Het
Utrn	A	G	10: 12,423,803 (GRCm39)		probably benign	Het
Wdr37	C	T	13: 8,885,406 (GRCm39)	D346N	probably damaging	Het
Zfpm2	T	A	15: 40,965,644 (GRCm39)	C710S	probably damaging	Het

Other mutations in Lats2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00272:Lats2	APN	14	57,929,026 (GRCm39)	missense	probably benign	0.09
IGL02104:Lats2	APN	14	57,971,469 (GRCm39)	missense	probably damaging	1.00
IGL02173:Lats2	APN	14	57,934,717 (GRCm39)	missense	probably damaging	1.00
IGL02377:Lats2	APN	14	57,929,052 (GRCm39)	missense	probably damaging	1.00
IGL02995:Lats2	APN	14	57,937,805 (GRCm39)	missense	probably damaging	1.00
Morpheus	UTSW	14	57,933,591 (GRCm39)	missense	probably damaging	1.00
PIT4472001:Lats2	UTSW	14	57,936,814 (GRCm39)	nonsense	probably null
R0653:Lats2	UTSW	14	57,937,653 (GRCm39)	nonsense	probably null
R0780:Lats2	UTSW	14	57,928,753 (GRCm39)	missense	probably damaging	1.00
R1129:Lats2	UTSW	14	57,937,790 (GRCm39)	missense	possibly damaging	0.71
R1851:Lats2	UTSW	14	57,934,912 (GRCm39)	missense	probably damaging	1.00
R1882:Lats2	UTSW	14	57,934,811 (GRCm39)	missense	probably damaging	1.00
R2184:Lats2	UTSW	14	57,929,016 (GRCm39)	missense	probably damaging	0.99
R3498:Lats2	UTSW	14	57,959,923 (GRCm39)	missense	possibly damaging	0.95
R3692:Lats2	UTSW	14	57,928,998 (GRCm39)	missense	probably damaging	1.00
R4212:Lats2	UTSW	14	57,933,712 (GRCm39)	missense	possibly damaging	0.82
R4357:Lats2	UTSW	14	57,936,840 (GRCm39)	missense	probably damaging	1.00
R4962:Lats2	UTSW	14	57,937,049 (GRCm39)	missense	probably damaging	1.00
R5394:Lats2	UTSW	14	57,928,810 (GRCm39)	missense	probably benign	0.10
R5477:Lats2	UTSW	14	57,937,010 (GRCm39)	missense	probably benign	0.00
R5729:Lats2	UTSW	14	57,960,192 (GRCm39)	missense	probably benign	0.04
R5802:Lats2	UTSW	14	57,931,875 (GRCm39)	missense	probably damaging	0.99
R5931:Lats2	UTSW	14	57,933,588 (GRCm39)	missense	probably damaging	1.00
R6016:Lats2	UTSW	14	57,971,632 (GRCm39)	missense	probably damaging	1.00
R6376:Lats2	UTSW	14	57,959,966 (GRCm39)	missense	probably benign	0.00
R6624:Lats2	UTSW	14	57,931,769 (GRCm39)	critical splice donor site	probably null
R6638:Lats2	UTSW	14	57,936,822 (GRCm39)	missense	probably damaging	1.00
R6846:Lats2	UTSW	14	57,933,591 (GRCm39)	missense	probably damaging	1.00
R7198:Lats2	UTSW	14	57,934,582 (GRCm39)	missense	probably damaging	1.00
R7233:Lats2	UTSW	14	57,960,151 (GRCm39)	splice site	probably null
R7883:Lats2	UTSW	14	57,934,657 (GRCm39)	missense	probably damaging	1.00
R8356:Lats2	UTSW	14	57,934,867 (GRCm39)	missense	probably damaging	1.00
R8508:Lats2	UTSW	14	57,960,162 (GRCm39)	missense	probably benign	0.08
R8536:Lats2	UTSW	14	57,940,495 (GRCm39)	missense	probably damaging	1.00
R8767:Lats2	UTSW	14	57,931,781 (GRCm39)	missense	probably damaging	1.00
R9579:Lats2	UTSW	14	57,937,191 (GRCm39)	missense	probably damaging	1.00
R9643:Lats2	UTSW	14	57,936,875 (GRCm39)	missense	possibly damaging	0.94

Predicted Primers

PCR Primer
(F):5'- ATAGTGTGTGCCCGGAAAG -3'
(R):5'- TGGTACTGGACATACCCTGC -3'

Sequencing Primer
(F):5'- AGTGCGCACTTGGCTCTACTG -3'
(R):5'- CCGGGAATCTTGAGAAGACCTTG -3'

Posted On

2020-06-30