Mutagenetix > Incidental Mutation

Incidental Mutation 'R8086:Mettl16'

629667

Institutional Source

Beutler Lab

Gene Symbol

Mettl16

Ensembl Gene

ENSMUSG00000010554

Gene Name

methyltransferase 16, N6-methyladenosine

Synonyms

2810013M15Rik, 2610100D03Rik, A830095F14Rik, Mett10d

MMRRC Submission

067519-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R8086 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

74661658-74716649 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 74696091 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 311 (T311A)

Ref Sequence

ENSEMBL: ENSMUSP00000010698 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000010698] [ENSMUST00000092912] [ENSMUST00000141755]

AlphaFold

Q9CQG2

Predicted Effect

probably benign
Transcript: ENSMUST00000010698
AA Change: T311A

PolyPhen 2 Score 0.026 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000010698
Gene: ENSMUSG00000010554
AA Change: T311A

Domain	Start	End	E-Value	Type
Pfam:Methyltransf_10	1	268	2.9e-114	PFAM
Pfam:MTS	92	202	1.8e-7	PFAM
Pfam:Methyltransf_10	276	331	5.5e-21	PFAM
low complexity region	421	437	N/A	INTRINSIC
low complexity region	497	518	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000092912

SMART Domains

Protein: ENSMUSP00000090590
Gene: ENSMUSG00000010554

Domain	Start	End	E-Value	Type
Pfam:Methyltransf_10	1	211	7.8e-97	PFAM
Pfam:PrmA	85	185	6.5e-7	PFAM
Pfam:MTS	92	200	1.4e-6	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000141755
AA Change: T271A

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000114682
Gene: ENSMUSG00000010554
AA Change: T271A

Domain	Start	End	E-Value	Type
Pfam:Methyltransf_10	1	291	4.8e-138	PFAM
low complexity region	381	397	N/A	INTRINSIC
low complexity region	457	478	N/A	INTRINSIC

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 98.9%
20x: 96.4%

Validation Efficiency

98% (51/52)

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2010315B03Rik	T	A	9: 124,055,808 (GRCm39)	H372L		Het
Abcb1a	A	T	5: 8,724,833 (GRCm39)	T89S	probably benign	Het
Ago1	A	G	4: 126,354,774 (GRCm39)	V146A	probably benign	Het
App	T	C	16: 84,917,428 (GRCm39)	Y72C	unknown	Het
Arhgap20	T	C	9: 51,760,563 (GRCm39)	S805P	probably benign	Het
Bak1	T	A	17: 27,239,911 (GRCm39)	R208S	probably benign	Het
Btnl4	A	C	17: 34,692,976 (GRCm39)		probably null	Het
Cacng7	T	C	7: 3,387,518 (GRCm39)	S134P	probably benign	Het
Capn9	A	T	8: 125,334,692 (GRCm39)		probably null	Het
Cox4i1	T	A	8: 121,400,779 (GRCm39)	M148K	probably damaging	Het
Ctnna1	A	G	18: 35,285,713 (GRCm39)	I20V	possibly damaging	Het
Dennd2c	T	A	3: 103,040,661 (GRCm39)	Y309N	possibly damaging	Het
Dnah14	C	A	1: 181,593,797 (GRCm39)	T3380K	probably damaging	Het
Dnajc3	G	T	14: 119,208,192 (GRCm39)	E276*	probably null	Het
Dock10	C	A	1: 80,481,707 (GRCm39)	C1772F	probably benign	Het
Fank1	A	T	7: 133,454,959 (GRCm39)	E26D	possibly damaging	Het
Fcgbp	G	T	7: 27,813,389 (GRCm39)	C2308F	probably damaging	Het
Fyco1	A	T	9: 123,659,471 (GRCm39)	M235K	probably damaging	Het
Gm7361	C	T	5: 26,465,446 (GRCm39)	R148C	probably damaging	Het
Hinfp	C	T	9: 44,210,286 (GRCm39)	R183Q	probably damaging	Het
Hpd	T	C	5: 123,314,252 (GRCm39)	Y221C	probably benign	Het
Hrnr	A	T	3: 93,230,728 (GRCm39)	H322L	unknown	Het
Il6st	T	A	13: 112,631,094 (GRCm39)		probably null	Het
Impa1	T	C	3: 10,387,988 (GRCm39)	K145E	probably benign	Het
Itga9	A	T	9: 118,679,869 (GRCm39)	M847L	probably benign	Het
Itgb6	A	G	2: 60,480,376 (GRCm39)	V320A	probably damaging	Het
Lrrfip1	T	A	1: 91,043,630 (GRCm39)	H678Q	probably benign	Het
Nefl	T	C	14: 68,323,480 (GRCm39)	Y369H	probably damaging	Het
Or1d2	C	A	11: 74,255,780 (GRCm39)	P95Q	probably benign	Het
Pkd1	A	G	17: 24,800,188 (GRCm39)	Y2983C	probably damaging	Het
Potegl	A	G	2: 23,130,934 (GRCm39)		probably null	Het
Prr5l	C	T	2: 101,571,709 (GRCm39)	E123K	probably benign	Het
Ptprq	A	T	10: 107,482,500 (GRCm39)	Y1024*	probably null	Het
Ramp2	T	A	11: 101,138,762 (GRCm39)	L147Q	probably damaging	Het
Rassf1	C	T	9: 107,435,173 (GRCm39)	R223C	probably benign	Het
Rcbtb2	T	C	14: 73,411,305 (GRCm39)	F357L	probably damaging	Het
Rnf24	A	G	2: 131,145,468 (GRCm39)	V114A	probably benign	Het
Slc7a1	T	G	5: 148,288,899 (GRCm39)	N116T	probably damaging	Het
Sstr2	T	C	11: 113,515,998 (GRCm39)	C306R	probably damaging	Het
Tatdn2	T	C	6: 113,686,482 (GRCm39)	S697P	probably damaging	Het
Tmem67	T	A	4: 12,040,738 (GRCm39)	N935I	probably damaging	Het
Trp73	G	A	4: 154,201,052 (GRCm39)	P4S	unknown	Het
Vmn1r237	T	G	17: 21,534,509 (GRCm39)	D77E	possibly damaging	Het
Vmn1r238	C	T	18: 3,123,250 (GRCm39)	A55T	probably damaging	Het
Vsig10l	C	A	7: 43,114,876 (GRCm39)	A359E	possibly damaging	Het
Wdr24	T	C	17: 26,045,101 (GRCm39)	Y279H	probably damaging	Het
Zbtb25	A	G	12: 76,395,923 (GRCm39)	V433A	probably benign	Het
Zfp180	C	A	7: 23,805,535 (GRCm39)	D651E	probably benign	Het
Zfp979	T	C	4: 147,698,004 (GRCm39)	D235G	probably damaging	Het

Other mutations in Mettl16

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00945:Mettl16	APN	11	74,708,192 (GRCm39)	missense	probably benign	0.11
IGL01538:Mettl16	APN	11	74,683,093 (GRCm39)	nonsense	probably null
IGL01893:Mettl16	APN	11	74,696,097 (GRCm39)	missense	possibly damaging	0.84
IGL02079:Mettl16	APN	11	74,708,450 (GRCm39)	missense	probably damaging	1.00
IGL03217:Mettl16	APN	11	74,708,529 (GRCm39)	missense	probably damaging	1.00
R2014:Mettl16	UTSW	11	74,708,195 (GRCm39)	missense	probably benign
R2117:Mettl16	UTSW	11	74,693,755 (GRCm39)	missense	probably benign
R4042:Mettl16	UTSW	11	74,683,118 (GRCm39)	missense	probably damaging	1.00
R4773:Mettl16	UTSW	11	74,708,127 (GRCm39)	missense	possibly damaging	0.49
R5031:Mettl16	UTSW	11	74,693,825 (GRCm39)	missense	probably benign
R5056:Mettl16	UTSW	11	74,707,766 (GRCm39)	missense	probably benign	0.34
R5951:Mettl16	UTSW	11	74,686,823 (GRCm39)	missense	possibly damaging	0.71
R5986:Mettl16	UTSW	11	74,683,063 (GRCm39)	missense	possibly damaging	0.51
R6012:Mettl16	UTSW	11	74,678,474 (GRCm39)	missense	probably damaging	1.00
R6314:Mettl16	UTSW	11	74,686,832 (GRCm39)	nonsense	probably null
R6450:Mettl16	UTSW	11	74,696,164 (GRCm39)	missense	probably benign	0.02
R7744:Mettl16	UTSW	11	74,693,829 (GRCm39)	missense	probably benign	0.08
R7959:Mettl16	UTSW	11	74,707,852 (GRCm39)	missense	probably benign	0.08
R8160:Mettl16	UTSW	11	74,708,505 (GRCm39)	missense	probably damaging	0.99
R9180:Mettl16	UTSW	11	74,693,826 (GRCm39)	missense	probably benign	0.21
R9257:Mettl16	UTSW	11	74,708,127 (GRCm39)	missense	possibly damaging	0.49
R9327:Mettl16	UTSW	11	74,696,089 (GRCm39)	missense	probably benign	0.25
R9496:Mettl16	UTSW	11	74,707,696 (GRCm39)	missense	possibly damaging	0.51
X0012:Mettl16	UTSW	11	74,707,851 (GRCm39)	missense	probably benign	0.02
X0028:Mettl16	UTSW	11	74,707,870 (GRCm39)	critical splice donor site	probably null

Predicted Primers

PCR Primer
(F):5'- CAACAGGAGTTAGCAGCCAG -3'
(R):5'- CCATAAACATCTGGATAAAACTTGAGG -3'

Sequencing Primer
(F):5'- CACGAGTAGAGAGGCTTTAGTGTG -3'
(R):5'- ACATCTGGATAAAACTTGAGGAAAAC -3'

Posted On

2020-06-30