Mutagenetix > Incidental Mutations

Incidental Mutation 'R8088:Mettl4'

629815

Institutional Source

Beutler Lab

Gene Symbol

Mettl4

Ensembl Gene

ENSMUSG00000055660

Gene Name

methyltransferase like 4

Synonyms

HsT661, A730091E08Rik, 2410198H06Rik

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.216) question?

Stock #

R8088 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

94727080-94749892 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 94735367 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 347 (V347A)

Ref Sequence

ENSEMBL: ENSMUSP00000127142 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171284]

AlphaFold

Q3U034

Predicted Effect

probably damaging
Transcript: ENSMUST00000171284
AA Change: V347A

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000127142
Gene: ENSMUSG00000055660
AA Change: V347A

Domain	Start	End	E-Value	Type
Pfam:MT-A70	280	454	9.9e-43	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.1%
20x: 96.8%

Validation Efficiency

100% (69/69)

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2410089E03Rik	A	G	15: 8,186,318	M453V	probably benign	Het
Atp2c1	A	T	9: 105,452,569		probably null	Het
Ccdc134	T	A	15: 82,131,789		probably benign	Het
Ccdc162	G	T	10: 41,623,414	H1066Q	possibly damaging	Het
Ccna1	A	G	3: 55,051,071	S64P	probably benign	Het
Defb12	T	C	8: 19,112,821		probably null	Het
Dhx9	C	A	1: 153,462,697	V738L	probably benign	Het
Dnah10	A	G	5: 124,754,266	N843S	probably benign	Het
Dspp	A	G	5: 104,177,256	D495G	unknown	Het
Dus2	A	G	8: 106,030,441	M88V	probably benign	Het
Dusp13	A	G	14: 21,741,237	V49A	probably benign	Het
Ehbp1	G	A	11: 22,089,572	H843Y	probably null	Het
Entpd3	A	C	9: 120,554,173	R91S	probably benign	Het
Fam207a	T	C	10: 77,514,395	N53S	probably benign	Het
Fam83c	C	A	2: 155,831,639	V210F	probably damaging	Het
Flot1	G	A	17: 35,829,978	A287T	probably damaging	Het
Gm11639	A	T	11: 104,998,246	I4163L	probably benign	Het
Gm19410	A	G	8: 35,806,841	K1412E	probably benign	Het
Gsn	G	A	2: 35,292,647	V241M	possibly damaging	Het
Gstcd	C	T	3: 133,082,107	V277M	probably damaging	Het
Heatr5a	T	C	12: 51,947,996	D451G	possibly damaging	Het
Hmcn2	G	A	2: 31,426,903	W3831*	probably null	Het
Il3	A	G	11: 54,265,549	V119A	probably benign	Het
Jam3	T	C	9: 27,098,860	K276R	probably benign	Het
Kcnt1	G	A	2: 25,894,314	G277D	possibly damaging	Het
Kif28	T	A	1: 179,700,354	D744V	probably damaging	Het
Ldlrad1	G	A	4: 107,209,491	A8T	probably benign	Het
Loxhd1	T	C	18: 77,342,013	I535T	possibly damaging	Het
Lzts1	A	C	8: 69,135,822	L494R	probably benign	Het
Mff	A	G	1: 82,751,649	E270G	probably damaging	Het
Mroh2b	A	T	15: 4,900,503	I24F	possibly damaging	Het
Muc16	A	T	9: 18,519,300	Y8102*	probably null	Het
Myh14	A	T	7: 44,665,496	M1K	probably null	Het
Myo18b	T	A	5: 112,879,510		probably benign	Het
Neurl1a	C	A	19: 47,257,434	P502T	probably damaging	Het
Nrp1	G	A	8: 128,468,516	W484*	probably null	Het
Olfr131	T	A	17: 38,082,561	H139L	possibly damaging	Het
Olfr1317	T	A	2: 112,142,257	I104N	probably benign	Het
Pcdhac1	T	C	18: 37,091,754	V540A	possibly damaging	Het
Pcdhga9	A	G	18: 37,736,954		probably benign	Het
Pm20d1	A	C	1: 131,801,763	N176T	probably benign	Het
Pnldc1	T	C	17: 12,897,302	D271G	probably damaging	Het
Pold3	A	G	7: 100,112,301	V63A	probably damaging	Het
Rbm18	A	C	2: 36,127,184	S61A	probably benign	Het
Rdh14	T	C	12: 10,394,551	L134P	probably damaging	Het
Repin1	G	T	6: 48,597,345	E403*	probably null	Het
Rtel1	A	G	2: 181,322,345	T46A	probably damaging	Het
Rtn3	T	C	19: 7,434,998	I823V	probably damaging	Het
S1pr1	A	G	3: 115,712,034	S304P	probably damaging	Het
Scfd2	A	G	5: 74,531,363	V86A	probably benign	Het
Scgb1a1	C	T	19: 9,085,235	V66M	probably damaging	Het
Sec31a	A	T	5: 100,378,862	M46K		Het
Sgsm1	A	G	5: 113,255,268	M971T	probably damaging	Het
Slc5a11	T	C	7: 123,265,728	I419T	probably benign	Het
Slco6d1	A	T	1: 98,466,706	T372S	possibly damaging	Het
Spata31	T	A	13: 64,920,865	Y276N	probably benign	Het
Sprr2k	C	T	3: 92,433,489	R49W	unknown	Het
Sspo	G	A	6: 48,457,613	C1013Y	probably damaging	Het
Ssr2	A	G	3: 88,579,883	R2G	possibly damaging	Het
St18	C	A	1: 6,828,005	T677K	probably benign	Het
Thap11	A	T	8: 105,855,895	I179F	probably damaging	Het
Trf	C	A	9: 103,211,931	G586C	probably damaging	Het
Umodl1	A	G	17: 30,973,796	N299S	probably benign	Het
Xrcc5	C	A	1: 72,312,436	A55E	probably damaging	Het

Other mutations in Mettl4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03376:Mettl4	APN	17	94735371	missense	probably damaging	1.00
R1938:Mettl4	UTSW	17	94747857	missense	possibly damaging	0.76
R2172:Mettl4	UTSW	17	94733163	missense	probably benign
R2370:Mettl4	UTSW	17	94733148	missense	probably damaging	1.00
R3621:Mettl4	UTSW	17	94735508	missense	probably damaging	1.00
R3913:Mettl4	UTSW	17	94740532	missense	probably benign	0.06
R4155:Mettl4	UTSW	17	94740575	missense	probably benign
R4536:Mettl4	UTSW	17	94735505	missense	possibly damaging	0.79
R4946:Mettl4	UTSW	17	94740532	missense	probably benign	0.06
R5263:Mettl4	UTSW	17	94740509	nonsense	probably null
R5397:Mettl4	UTSW	17	94727277	nonsense	probably null
R6242:Mettl4	UTSW	17	94735374	missense	probably damaging	1.00
R6508:Mettl4	UTSW	17	94743945	missense	probably damaging	0.98
R7069:Mettl4	UTSW	17	94733633	missense	probably damaging	0.98
R7941:Mettl4	UTSW	17	94733194	splice site	probably null
R8373:Mettl4	UTSW	17	94733649	missense	probably damaging	1.00
R8710:Mettl4	UTSW	17	94733644	missense	probably damaging	0.99
R9055:Mettl4	UTSW	17	94740415	missense	possibly damaging	0.65
R9130:Mettl4	UTSW	17	94735485	missense	possibly damaging	0.61
R9335:Mettl4	UTSW	17	94735508	missense	probably damaging	1.00
Z1176:Mettl4	UTSW	17	94733563	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GTGTTCAGATACAAAGCATGAACAG -3'
(R):5'- ATTTAGCTGCATGTGAGCGG -3'

Sequencing Primer
(F):5'- GCATGAACAGTTCTTGTCAGAGCC -3'
(R):5'- TGCTGCTGACTGTCTCAT -3'

Posted On

2020-06-30