Mutagenetix > Incidental Mutation

Incidental Mutation 'R8099:Vmn2r8'

630389

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r8

Ensembl Gene

ENSMUSG00000090961

Gene Name

vomeronasal 2, receptor 8

Synonyms

EG627479

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R8099 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

108797193-108808754 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 108801834 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 382 (N382K)

Ref Sequence

ENSEMBL: ENSMUSP00000126953 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000172140]

AlphaFold

L7N472

Predicted Effect

probably benign
Transcript: ENSMUST00000172140
AA Change: N382K

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000126953
Gene: ENSMUSG00000090961
AA Change: N382K

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	78	419	1.1e-28	PFAM
Pfam:NCD3G	507	561	8.2e-18	PFAM
Pfam:7tm_3	594	829	1.1e-54	PFAM

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.3%
20x: 98.1%

Validation Efficiency

97% (60/62)

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9930012K11Rik	T	C	14: 70,157,520	R62G	probably benign	Het
Abcb9	A	G	5: 124,077,245	F478L	probably benign	Het
Abcc9	A	T	6: 142,675,531	N494K	probably damaging	Het
Atp11a	T	G	8: 12,861,973	M124R		Het
C1ra	G	A	6: 124,517,725	E316K	probably benign	Het
Ccdc110	T	A	8: 45,942,093	H340Q	probably damaging	Het
Celsr1	A	G	15: 86,031,600	L724P	probably damaging	Het
Cfap74	A	G	4: 155,455,032	N938S	unknown	Het
Chil3	T	G	3: 106,148,668	D363A	probably damaging	Het
Ciart	G	A	3: 95,881,344	P61L	probably damaging	Het
Cpne3	A	G	4: 19,525,169	V442A	possibly damaging	Het
Ctsh	T	C	9: 90,064,247	V130A	probably damaging	Het
Cyp3a44	T	C	5: 145,788,402	K330E	probably benign	Het
Dgcr2	A	T	16: 17,849,769	D275E	probably damaging	Het
Dnah1	A	T	14: 31,302,364	D919E	probably benign	Het
Ell3	TCTCCTC	TCTC	2: 121,439,456		probably benign	Het
Epb41l3	T	A	17: 69,247,688	F261Y	possibly damaging	Het
Fam189b	T	C	3: 89,183,943	F74L	probably damaging	Het
Fbxo3	A	C	2: 104,054,935	H383P	probably damaging	Het
Foxp1	C	T	6: 98,945,549	V441M	unknown	Het
Gm29106	C	T	1: 118,198,791	S71F	probably benign	Het
Golga3	T	A	5: 110,188,707	L364*	probably null	Het
Herc1	T	A	9: 66,372,140	L110H	probably damaging	Het
Hist1h1a	C	T	13: 23,763,849	L45F	probably damaging	Het
Igkv4-53	A	T	6: 69,648,914	S90T	possibly damaging	Het
Kctd17	CAGCTGGAGGAGC	CAGC	15: 78,436,913		probably benign	Het
Kti12	G	T	4: 108,848,374	G162W	probably damaging	Het
Lama3	T	A	18: 12,534,063	I763N	probably damaging	Het
Macf1	A	T	4: 123,476,129	I1613K	probably benign	Het
Mkrn1	T	C	6: 39,410,097	D102G	probably benign	Het
Muc2	T	A	7: 141,745,438		probably null	Het
Nipal4	T	C	11: 46,162,021	E39G	probably benign	Het
Nr1h2	T	C	7: 44,550,322	Y391C	possibly damaging	Het
Odam	T	C	5: 87,892,440	I255T	possibly damaging	Het
Olfr1154	T	A	2: 87,903,508	H56L	probably damaging	Het
Olfr279	A	G	15: 98,497,813	M114V	probably benign	Het
Olfr781	T	A	10: 129,333,127	I82K	probably damaging	Het
Per3	A	T	4: 151,012,557	M837K	possibly damaging	Het
Pitpnm3	T	A	11: 72,070,318	H357L	possibly damaging	Het
Pkd1l3	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	8: 109,624,195		probably benign	Het
Plcb1	T	C	2: 135,251,734	Y156H	possibly damaging	Het
Ppm1d	C	T	11: 85,339,666	P370L	possibly damaging	Het
Prrc2b	T	C	2: 32,208,674	V666A	probably benign	Het
Ptpn3	A	T	4: 57,204,985	C725*	probably null	Het
Rapgefl1	T	C	11: 98,847,383	W437R	probably damaging	Het
Sema4g	T	C	19: 44,992,528	L5P	probably damaging	Het
Sipa1l1	C	T	12: 82,433,826	P1529L	probably benign	Het
Slc12a2	T	C	18: 57,899,392	L388P	probably damaging	Het
Sltm	T	A	9: 70,586,078	S786T	probably damaging	Het
Srsf1	T	C	11: 88,049,256	I198T	probably benign	Het
Ssh2	C	T	11: 77,454,929	R1247*	probably null	Het
Ssu2	T	C	6: 112,376,477	T229A	probably benign	Het
Stra6	T	A	9: 58,152,494	H579Q	probably damaging	Het
Tanc2	T	A	11: 105,864,007	V559E	probably benign	Het
Trp53bp1	A	G	2: 121,199,749	Y1762H	probably damaging	Het
Usp15	T	C	10: 123,146,921	Y263C	possibly damaging	Het
Usp17ld	A	T	7: 103,250,288	V479E	probably damaging	Het
Vmn2r15	A	T	5: 109,293,319	S224R	possibly damaging	Het
Vmn2r94	T	C	17: 18,257,397	I251V	probably benign	Het
Wnt2b	A	T	3: 104,947,092	V350D	possibly damaging	Het

Other mutations in Vmn2r8

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02957:Vmn2r8	APN	5	108802225	missense	probably benign	0.01
R0324:Vmn2r8	UTSW	5	108797941	splice site	probably null
R0335:Vmn2r8	UTSW	5	108797451	splice site	probably null
R0394:Vmn2r8	UTSW	5	108802072	missense	probably benign	0.12
R0501:Vmn2r8	UTSW	5	108803183	missense	probably benign	0.03
R0615:Vmn2r8	UTSW	5	108799329	missense	probably damaging	1.00
R0678:Vmn2r8	UTSW	5	108800546	missense	probably benign	0.00
R1167:Vmn2r8	UTSW	5	108803176	missense	probably benign	0.01
R1187:Vmn2r8	UTSW	5	108803219	nonsense	probably null
R1406:Vmn2r8	UTSW	5	108802368	missense	probably benign
R1406:Vmn2r8	UTSW	5	108802368	missense	probably benign
R1451:Vmn2r8	UTSW	5	108798067	missense	probably damaging	1.00
R1535:Vmn2r8	UTSW	5	108802174	missense	probably damaging	1.00
R1795:Vmn2r8	UTSW	5	108803106	missense	probably benign
R1874:Vmn2r8	UTSW	5	108802418	missense	possibly damaging	0.74
R1908:Vmn2r8	UTSW	5	108797570	missense	probably benign	0.03
R1925:Vmn2r8	UTSW	5	108802153	missense	probably damaging	0.97
R1960:Vmn2r8	UTSW	5	108799286	missense	probably damaging	0.99
R1961:Vmn2r8	UTSW	5	108798095	missense	probably benign	0.45
R1967:Vmn2r8	UTSW	5	108802383	missense	probably benign	0.01
R2095:Vmn2r8	UTSW	5	108808621	missense	possibly damaging	0.94
R2159:Vmn2r8	UTSW	5	108802303	missense	probably benign	0.22
R4240:Vmn2r8	UTSW	5	108797503	missense	probably damaging	0.99
R4581:Vmn2r8	UTSW	5	108801704	missense	probably benign	0.03
R4744:Vmn2r8	UTSW	5	108808581	missense	probably benign	0.00
R4755:Vmn2r8	UTSW	5	108801700	missense	probably benign	0.03
R4917:Vmn2r8	UTSW	5	108797398	missense	probably damaging	1.00
R4957:Vmn2r8	UTSW	5	108799263	missense	probably benign	0.16
R5141:Vmn2r8	UTSW	5	108808706	missense	probably damaging	0.96
R5481:Vmn2r8	UTSW	5	108801770	missense	probably benign	0.09
R5571:Vmn2r8	UTSW	5	108802240	missense	probably damaging	1.00
R5624:Vmn2r8	UTSW	5	108802459	missense	probably damaging	0.99
R6003:Vmn2r8	UTSW	5	108797382	missense	probably damaging	1.00
R6243:Vmn2r8	UTSW	5	108799345	missense	probably benign	0.01
R6265:Vmn2r8	UTSW	5	108808597	missense	probably benign
R6315:Vmn2r8	UTSW	5	108801891	missense	probably benign
R6413:Vmn2r8	UTSW	5	108801723	missense	probably benign	0.09
R7120:Vmn2r8	UTSW	5	108808638	missense	possibly damaging	0.56
R7406:Vmn2r8	UTSW	5	108800576	missense	probably benign	0.00
R7409:Vmn2r8	UTSW	5	108808583	nonsense	probably null
R7489:Vmn2r8	UTSW	5	108797656	missense	possibly damaging	0.95
R7532:Vmn2r8	UTSW	5	108802240	missense	probably benign	0.22
R7534:Vmn2r8	UTSW	5	108802174	missense	possibly damaging	0.94
R7739:Vmn2r8	UTSW	5	108802177	missense	probably damaging	1.00
R8245:Vmn2r8	UTSW	5	108798070	missense	probably damaging	1.00
R8711:Vmn2r8	UTSW	5	108798096	missense	possibly damaging	0.89
R8781:Vmn2r8	UTSW	5	108797731	missense	possibly damaging	0.95
R8874:Vmn2r8	UTSW	5	108808751	missense	probably damaging	1.00
R8927:Vmn2r8	UTSW	5	108802265	missense
R8928:Vmn2r8	UTSW	5	108802265	missense
R9288:Vmn2r8	UTSW	5	108802319	missense	probably benign	0.39
R9596:Vmn2r8	UTSW	5	108799330	missense	possibly damaging	0.94
R9652:Vmn2r8	UTSW	5	108803241	missense	probably benign	0.18
Z1088:Vmn2r8	UTSW	5	108801998	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TGGGTCCGTTTAAAAGATCCCATTG -3'
(R):5'- ACTTTTGAACACCACCACAGTG -3'

Sequencing Primer
(F):5'- CTGCTGACAGTCAGTGAA -3'
(R):5'- ACCACAGTGAGATTGCTAAATTTAAG -3'

Posted On

2020-06-30