Mutagenetix > Incidental Mutation

Incidental Mutation 'R8108:Or4g7'

630712

Institutional Source

Beutler Lab

Gene Symbol

Or4g7

Ensembl Gene

ENSMUSG00000044039

Gene Name

olfactory receptor family 4 subfamily G member 7

Synonyms

Olfr1288, GA_x6K02T2Q125-72530279-72531217, MOR245-9

MMRRC Submission

067537-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.071) question?

Stock #

R8108 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

111474101-111479994 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 111479234 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 150 (V150A)

Ref Sequence

ENSEMBL: ENSMUSP00000150331 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000104889] [ENSMUST00000120021] [ENSMUST00000207494] [ENSMUST00000214816]

AlphaFold

Q7TQY0

Predicted Effect

possibly damaging
Transcript: ENSMUST00000104889
AA Change: V150A

PolyPhen 2 Score 0.897 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000100485
Gene: ENSMUSG00000044039
AA Change: V150A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	304	1.3e-43	PFAM
Pfam:7tm_1	41	287	2.5e-20	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000120021

Predicted Effect

probably benign
Transcript: ENSMUST00000207494

Predicted Effect

possibly damaging
Transcript: ENSMUST00000214816
AA Change: V150A

PolyPhen 2 Score 0.897 (Sensitivity: 0.82; Specificity: 0.94)

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 99.0%
20x: 97.3%

Validation Efficiency

99% (72/73)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl4fm4	A	T	4: 144,670,669 (GRCm38)	N164K	probably benign	Het
Acot1	T	A	12: 84,017,361 (GRCm38)	H414Q	probably benign	Het
Actrt2	A	T	4: 154,667,036 (GRCm38)	D214E	probably benign	Het
Afg2a	T	C	3: 37,431,782 (GRCm38)	S218P	probably benign	Het
Ccdc7a	T	C	8: 128,980,153 (GRCm38)	T332A	unknown	Het
Chd9	C	T	8: 90,933,224 (GRCm38)	H271Y	unknown	Het
Depdc5	T	G	5: 32,945,049 (GRCm38)	D966E	probably benign	Het
Dolpp1	A	G	2: 30,396,246 (GRCm38)	Y119C	probably benign	Het
Dscam	G	C	16: 96,643,879 (GRCm38)	D1537E	probably benign	Het
Dusp16	A	G	6: 134,739,873 (GRCm38)	I157T	probably benign	Het
Dyrk2	T	C	10: 118,859,829 (GRCm38)	D508G	probably benign	Het
Endov	T	C	11: 119,507,411 (GRCm38)	V334A	probably benign	Het
Ep400	A	T	5: 110,687,883 (GRCm38)	V1956E	unknown	Het
Erich3	A	T	3: 154,720,115 (GRCm38)	Y83F	possibly damaging	Het
Fbrsl1	T	C	5: 110,378,379 (GRCm38)		probably null	Het
Gm1110	T	C	9: 26,920,661 (GRCm38)	I65V	probably damaging	Het
Gm28374	A	G	19: 6,082,200 (GRCm38)	V19A		Het
Gpr107	C	A	2: 31,184,869 (GRCm38)	H336Q	probably damaging	Het
Gpr137b	T	C	13: 13,359,406 (GRCm38)	Y355C		Het
Grm1	C	A	10: 10,720,132 (GRCm38)	R584L	probably benign	Het
Gse1	T	C	8: 120,229,810 (GRCm38)	S347P	unknown	Het
Ier3ip1	T	A	18: 76,940,525 (GRCm38)	I69K	possibly damaging	Het
Impact	T	G	18: 12,984,331 (GRCm38)	L154V	probably benign	Het
Katnb1	T	C	8: 95,093,945 (GRCm38)	F141S	possibly damaging	Het
Kifap3	T	A	1: 163,797,362 (GRCm38)	N162K	probably damaging	Het
Klhl5	A	G	5: 65,148,587 (GRCm38)		probably null	Het
Klre1	A	G	6: 129,584,222 (GRCm38)	D182G	probably benign	Het
Krt42	T	A	11: 100,266,957 (GRCm38)	Y227F	probably benign	Het
Lhcgr	T	A	17: 88,742,050 (GRCm38)	K683*	probably null	Het
Lrrc37a	G	A	11: 103,503,057 (GRCm38)	S514F	probably benign	Het
Lrrc9	G	T	12: 72,454,059 (GRCm38)	L186F	probably damaging	Het
Metrn	A	G	17: 25,795,030 (GRCm38)	V274A	probably benign	Het
Mettl25	A	T	10: 105,823,179 (GRCm38)	F414L	possibly damaging	Het
Mixl1	A	G	1: 180,696,702 (GRCm38)	V104A	probably damaging	Het
Myo9b	T	A	8: 71,348,342 (GRCm38)	M1047K	probably damaging	Het
Nbea	G	A	3: 55,819,315 (GRCm38)	A2081V	probably benign	Het
Ndufs1	A	T	1: 63,150,012 (GRCm38)	D551E	possibly damaging	Het
Nectin3	T	C	16: 46,464,121 (GRCm38)	T67A	possibly damaging	Het
Nme8	C	T	13: 19,650,960 (GRCm38)	V519I	probably benign	Het
Npat	G	T	9: 53,571,129 (GRCm38)	G1379V	probably benign	Het
Obscn	T	A	11: 59,061,634 (GRCm38)	T3870S	probably benign	Het
Or1e20-ps1	T	A	11: 73,433,854 (GRCm38)	H124L	unknown	Het
Or5g23	T	C	2: 85,608,749 (GRCm38)	R54G	possibly damaging	Het
Or5k14	T	C	16: 58,872,236 (GRCm38)	M305V	probably benign	Het
Or7h8	T	A	9: 20,212,451 (GRCm38)	M34K	possibly damaging	Het
Or8b46	A	G	9: 38,539,410 (GRCm38)	N172D	probably damaging	Het
Parp8	A	T	13: 116,867,073 (GRCm38)	Y769*	probably null	Het
Pcnx1	C	T	12: 81,918,819 (GRCm38)	R59*	probably null	Het
Pdzd2	G	A	15: 12,373,506 (GRCm38)	S2181L	probably benign	Het
Phldb1	T	C	9: 44,711,161 (GRCm38)	E65G	probably damaging	Het
Ppif	C	T	14: 25,698,326 (GRCm38)	T157M	probably damaging	Het
Ppp3ca	T	A	3: 136,932,225 (GRCm38)		probably null	Het
Proser1	T	A	3: 53,472,088 (GRCm38)		probably null	Het
Reg3d	T	A	6: 78,376,079 (GRCm38)	K174*	probably null	Het
Rubcn	A	T	16: 32,856,950 (GRCm38)	L55Q	probably damaging	Het
Sec14l3	T	C	11: 4,066,198 (GRCm38)	L39P	probably damaging	Het
Ska3	A	G	14: 57,826,102 (GRCm38)	I4T	probably damaging	Het
Slc30a10	A	G	1: 185,464,154 (GRCm38)	I338V	possibly damaging	Het
Slc35g1	C	G	19: 38,402,829 (GRCm38)	S186R	probably damaging	Het
Slc35g1	T	A	19: 38,402,831 (GRCm38)	L187H	probably damaging	Het
Tbc1d5	T	C	17: 50,742,086 (GRCm38)	I659V	probably benign	Het
Tbl3	G	T	17: 24,700,916 (GRCm38)	D751E	probably benign	Het
Tdrd3	T	A	14: 87,486,266 (GRCm38)	D311E	possibly damaging	Het
Tenm4	T	C	7: 96,854,728 (GRCm38)	F1335S	probably benign	Het
Tm2d1	A	T	4: 98,375,023 (GRCm38)	C83S	probably damaging	Het
Tnfrsf22	A	T	7: 143,638,373 (GRCm38)	V192D	unknown	Het
Trpm6	A	G	19: 18,811,790 (GRCm38)	T575A	probably damaging	Het
Uri1	T	C	7: 37,981,673 (GRCm38)	D102G	possibly damaging	Het
Vmn2r1	T	A	3: 64,103,050 (GRCm38)	C570S	probably damaging	Het
Vps13a	C	T	19: 16,640,787 (GRCm38)	V2906I	probably damaging	Het
Zfp472	T	C	17: 32,978,003 (GRCm38)	Y351H	possibly damaging	Het
Zfp874a	G	T	13: 67,443,234 (GRCm38)	Y110*	probably null	Het
Zfp986	T	A	4: 145,899,305 (GRCm38)	N178K	probably benign	Het

Other mutations in Or4g7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01800:Or4g7	APN	2	111,478,864 (GRCm38)	missense	probably benign
IGL02021:Or4g7	APN	2	111,479,480 (GRCm38)	missense	probably benign	0.03
IGL02061:Or4g7	APN	2	111,479,269 (GRCm38)	missense	possibly damaging	0.94
R2016:Or4g7	UTSW	2	111,479,187 (GRCm38)	missense	probably benign	0.00
R2017:Or4g7	UTSW	2	111,479,187 (GRCm38)	missense	probably benign	0.00
R2848:Or4g7	UTSW	2	111,479,354 (GRCm38)	missense	probably benign	0.00
R2849:Or4g7	UTSW	2	111,479,354 (GRCm38)	missense	probably benign	0.00
R3421:Or4g7	UTSW	2	111,478,952 (GRCm38)	missense	probably benign	0.12
R4223:Or4g7	UTSW	2	111,479,144 (GRCm38)	missense	probably benign	0.00
R4432:Or4g7	UTSW	2	111,479,412 (GRCm38)	nonsense	probably null
R4433:Or4g7	UTSW	2	111,479,412 (GRCm38)	nonsense	probably null
R4476:Or4g7	UTSW	2	111,479,664 (GRCm38)	missense	possibly damaging	0.58
R4631:Or4g7	UTSW	2	111,479,563 (GRCm38)	missense	probably damaging	1.00
R6029:Or4g7	UTSW	2	111,478,965 (GRCm38)	nonsense	probably null
R6036:Or4g7	UTSW	2	111,478,988 (GRCm38)	missense	probably damaging	1.00
R6036:Or4g7	UTSW	2	111,478,988 (GRCm38)	missense	probably damaging	1.00
R6084:Or4g7	UTSW	2	111,479,389 (GRCm38)	missense	probably damaging	1.00
R6329:Or4g7	UTSW	2	111,479,228 (GRCm38)	missense	possibly damaging	0.90
R7307:Or4g7	UTSW	2	111,478,760 (GRCm38)	start gained	probably benign
R7516:Or4g7	UTSW	2	111,478,937 (GRCm38)	missense	probably benign	0.01
R7577:Or4g7	UTSW	2	111,479,132 (GRCm38)	missense	probably damaging	0.98
R8210:Or4g7	UTSW	2	111,479,408 (GRCm38)	missense	possibly damaging	0.89
R8465:Or4g7	UTSW	2	111,479,080 (GRCm38)	missense	probably benign	0.01
R8717:Or4g7	UTSW	2	111,479,647 (GRCm38)	missense	probably damaging	1.00
R8730:Or4g7	UTSW	2	111,479,589 (GRCm38)	missense	probably damaging	1.00
R9360:Or4g7	UTSW	2	111,479,339 (GRCm38)	missense	probably benign	0.10
Z1177:Or4g7	UTSW	2	111,479,207 (GRCm38)	missense	probably damaging	1.00
Z1177:Or4g7	UTSW	2	111,478,814 (GRCm38)	missense	probably benign	0.06

Predicted Primers

PCR Primer
(F):5'- TTCCACAGCTGCATGACAC -3'
(R):5'- GAATGTTGTCGTACACTGGCC -3'

Sequencing Primer
(F):5'- CAGCTGCATGACACAAATGTTCTTC -3'
(R):5'- TCGTACACTGGCCAGGATAAAAATG -3'

Posted On

2020-06-30