Mutagenetix > Incidental Mutation

Incidental Mutation 'R8111:Or10g3b'

630893

Institutional Source

Beutler Lab

Gene Symbol

Or10g3b

Ensembl Gene

ENSMUSG00000095030

Gene Name

olfactory receptor family 10 subfamily G member 3B

Synonyms

GA_x6K02T2RJGY-644134-645075, Olfr1513, MOR223-10, MOR223-7P

MMRRC Submission

067540-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.107) question?

Stock #

R8111 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

52586560-52587501 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 52587344 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Methionine at position 53 (T53M)

Ref Sequence

ENSEMBL: ENSMUSP00000149216 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000089739] [ENSMUST00000215147]

AlphaFold

L7N457

Predicted Effect

possibly damaging
Transcript: ENSMUST00000089739
AA Change: T53M

PolyPhen 2 Score 0.702 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000087171
Gene: ENSMUSG00000095030
AA Change: T53M

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	5.2e-49	PFAM
Pfam:7tm_1	41	291	1.6e-20	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000215147
AA Change: T53M

PolyPhen 2 Score 0.702 (Sensitivity: 0.86; Specificity: 0.92)

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.7%
20x: 93.2%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3425401B19Rik	C	T	14: 32,382,266 (GRCm39)	W1233*	probably null	Het
4930590J08Rik	A	G	6: 91,894,691 (GRCm39)	I247V	probably benign	Het
8030423J24Rik	T	A	13: 71,032,077 (GRCm39)	C50S	unknown	Het
Adam29	T	G	8: 56,324,585 (GRCm39)	H623P	probably benign	Het
Adamts5	A	G	16: 85,696,203 (GRCm39)	V318A	probably damaging	Het
Ano3	T	C	2: 110,614,058 (GRCm39)	D215G	possibly damaging	Het
Ap3b2	A	G	7: 81,113,530 (GRCm39)	I893T	unknown	Het
Apob	G	A	12: 8,058,801 (GRCm39)	A2428T	probably benign	Het
Armc3	T	C	2: 19,301,674 (GRCm39)	V660A	probably benign	Het
Atf7	G	T	15: 102,471,769 (GRCm39)	T42K	probably damaging	Het
Atg9a	A	T	1: 75,164,366 (GRCm39)	I160N	probably damaging	Het
Atp2b1	T	C	10: 98,832,786 (GRCm39)	V429A	possibly damaging	Het
Bpifb3	A	C	2: 153,764,609 (GRCm39)	H167P	probably benign	Het
Cacna1f	G	T	X: 7,487,326 (GRCm39)	E921D	probably damaging	Het
Ccdc57	T	A	11: 120,769,713 (GRCm39)	L713F	probably damaging	Het
Chd1l	C	T	3: 97,494,526 (GRCm39)	E385K	possibly damaging	Het
Csmd1	C	T	8: 15,967,306 (GRCm39)	V3186I	probably benign	Het
Dclre1c	T	A	2: 3,448,185 (GRCm39)	D349E	probably benign	Het
Dlg1	C	T	16: 31,661,620 (GRCm39)	T657M	possibly damaging	Het
Dync2h1	T	C	9: 7,148,688 (GRCm39)	I919V	probably benign	Het
Dync2i2	C	A	2: 29,921,859 (GRCm39)	A501S	possibly damaging	Het
Eml5	A	G	12: 98,758,773 (GRCm39)		probably null	Het
Epas1	C	A	17: 87,125,860 (GRCm39)	S286*	probably null	Het
Fat1	G	A	8: 45,479,095 (GRCm39)	V2714I	possibly damaging	Het
Fuca2	T	C	10: 13,390,545 (GRCm39)	M447T	probably benign	Het
Gm10800	CAAGAAAACTGAAAATCAAAGAAAACTGAAAATCA	CAAGAAAACTGAAAATCA	2: 98,497,361 (GRCm39)		probably null	Het
Gm11110	A	C	17: 57,410,427 (GRCm39)	C24G	probably null	Het
Gm6176	T	A	7: 21,750,593 (GRCm39)	I113F	probably benign	Het
Gmcl1	G	T	6: 86,698,408 (GRCm39)	A163E	probably damaging	Het
Gpat2	G	T	2: 127,275,777 (GRCm39)	L518F	probably damaging	Het
Gpr137b	T	C	13: 13,533,991 (GRCm39)	Y355C		Het
Hgsnat	C	T	8: 26,458,440 (GRCm39)	V195I	probably benign	Het
Hivep3	T	A	4: 119,955,583 (GRCm39)	S1300T	probably damaging	Het
Hs3st2	A	T	7: 120,992,362 (GRCm39)	H137L	probably damaging	Het
Iffo1	T	C	6: 125,122,781 (GRCm39)	S188P	possibly damaging	Het
Itih1	T	C	14: 30,654,225 (GRCm39)	D684G	probably damaging	Het
Lrba	C	A	3: 86,235,012 (GRCm39)	N852K	probably damaging	Het
Lrriq4	T	C	3: 30,709,930 (GRCm39)	S425P	possibly damaging	Het
Mdn1	T	A	4: 32,674,003 (GRCm39)	S562T	possibly damaging	Het
Mex3a	T	C	3: 88,444,064 (GRCm39)	V380A	probably benign	Het
Mgat4d	A	T	8: 84,094,776 (GRCm39)	N271I	probably damaging	Het
Mmp24	T	A	2: 155,649,345 (GRCm39)	V254E	possibly damaging	Het
Muc16	T	A	9: 18,503,925 (GRCm39)	R6455S	possibly damaging	Het
Npffr1	T	A	10: 61,459,128 (GRCm39)	V127E	probably damaging	Het
Obox5	T	A	7: 15,492,541 (GRCm39)	N165K	probably damaging	Het
Otulin	A	C	15: 27,606,381 (GRCm39)	V344G	probably damaging	Het
Pappa	A	G	4: 65,180,229 (GRCm39)	D1030G	probably damaging	Het
Pdzd2	G	A	15: 12,373,592 (GRCm39)	S2181L	probably benign	Het
Ppp2r3c	C	A	12: 55,344,634 (GRCm39)	M111I	probably benign	Het
Prss37	T	C	6: 40,494,747 (GRCm39)	T13A	probably benign	Het
Sfi1	TCGC	TC	11: 3,096,254 (GRCm39)		probably null	Het
Sgo2b	T	A	8: 64,396,138 (GRCm39)	K39N	probably damaging	Het
Sike1	T	C	3: 102,909,123 (GRCm39)	*208Q	probably null	Het
Spire1	A	G	18: 67,652,391 (GRCm39)	S229P	probably damaging	Het
Tmem132b	A	G	5: 125,699,857 (GRCm39)	I132V	probably benign	Het
Umodl1	T	C	17: 31,190,792 (GRCm39)	V213A	probably damaging	Het
Washc1	C	G	17: 66,423,033 (GRCm39)	Q116E	probably benign	Het
Zfp553	T	A	7: 126,836,093 (GRCm39)	C549*	probably null	Het
Zfp9	G	A	6: 118,441,561 (GRCm39)	P367L	probably damaging	Het

Other mutations in Or10g3b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00309:Or10g3b	APN	14	52,587,167 (GRCm39)	missense	probably benign	0.00
IGL02485:Or10g3b	APN	14	52,587,501 (GRCm39)	start codon destroyed	possibly damaging	0.89
IGL02828:Or10g3b	APN	14	52,586,799 (GRCm39)	missense	probably benign	0.02
R0744:Or10g3b	UTSW	14	52,586,835 (GRCm39)	missense	probably benign	0.00
R0833:Or10g3b	UTSW	14	52,586,835 (GRCm39)	missense	probably benign	0.00
R2316:Or10g3b	UTSW	14	52,587,395 (GRCm39)	missense	probably benign	0.00
R4898:Or10g3b	UTSW	14	52,586,999 (GRCm39)	missense	probably damaging	1.00
R5018:Or10g3b	UTSW	14	52,586,736 (GRCm39)	missense	possibly damaging	0.81
R5134:Or10g3b	UTSW	14	52,587,248 (GRCm39)	missense	probably benign	0.03
R5485:Or10g3b	UTSW	14	52,586,776 (GRCm39)	nonsense	probably null
R6819:Or10g3b	UTSW	14	52,587,156 (GRCm39)	missense	probably damaging	1.00
R6877:Or10g3b	UTSW	14	52,587,270 (GRCm39)	missense	possibly damaging	0.95
R7583:Or10g3b	UTSW	14	52,587,360 (GRCm39)	missense	possibly damaging	0.93
R7606:Or10g3b	UTSW	14	52,587,420 (GRCm39)	missense	probably benign	0.33
R7653:Or10g3b	UTSW	14	52,586,889 (GRCm39)	nonsense	probably null
R8262:Or10g3b	UTSW	14	52,586,625 (GRCm39)	missense	probably damaging	1.00
R8487:Or10g3b	UTSW	14	52,586,696 (GRCm39)	missense	probably damaging	1.00
R8786:Or10g3b	UTSW	14	52,587,021 (GRCm39)	missense	possibly damaging	0.74
R9003:Or10g3b	UTSW	14	52,586,768 (GRCm39)	missense	probably damaging	1.00
R9507:Or10g3b	UTSW	14	52,586,678 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTACTGCCCAGAAAGTGGTAG -3'
(R):5'- TGCCCCAGGTTTTCACAGTC -3'

Sequencing Primer
(F):5'- GTAGAAATAAAGCTGAGCCACGCATC -3'
(R):5'- AGCTTGCACGTCATTTATGCTAAC -3'

Posted On

2020-06-30