Mutagenetix > Incidental Mutation

Incidental Mutation 'R8113:Vmn2r109'

631033

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r109

Ensembl Gene

ENSMUSG00000090572

Gene Name

vomeronasal 2, receptor 109

Synonyms

EG627814

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.066) question?

Stock #

R8113 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

20540517-20564756 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 20554467 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 209 (M209L)

Ref Sequence

ENSEMBL: ENSMUSP00000132641 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167093]

AlphaFold

K7N747

Predicted Effect

probably benign
Transcript: ENSMUST00000167093
AA Change: M209L

PolyPhen 2 Score 0.011 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000132641
Gene: ENSMUSG00000090572
AA Change: M209L

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	83	467	1.4e-35	PFAM
Pfam:NCD3G	510	563	3.1e-21	PFAM
Pfam:7tm_3	596	831	7.4e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

96% (72/75)

Allele List at MGI

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy7	T	C	8: 88,321,803	S625P	probably damaging	Het
Agpat1	T	C	17: 34,611,612	V170A	probably damaging	Het
Alg9	C	T	9: 50,808,780	Q519*	probably null	Het
Apol8	C	T	15: 77,750,136	R80H	probably benign	Het
Arhgap25	G	T	6: 87,488,305	S118*	probably null	Het
Arhgap30	T	A	1: 171,397,518	Y47*	probably null	Het
Arhgap42	C	T	9: 9,011,433	D451N	probably damaging	Het
Cabp2	T	A	19: 4,085,582	F91I	probably damaging	Het
Ccdc30	T	C	4: 119,373,746	E108G	probably benign	Het
Cfap99	T	C	5: 34,301,419	S53P	probably damaging	Het
Clec5a	C	T	6: 40,579,427	R135H	possibly damaging	Het
Cops8	T	G	1: 90,603,603	D18E	probably benign	Het
Cped1	A	T	6: 22,233,481	M779L	possibly damaging	Het
Cyp4x1	G	A	4: 115,110,066	H438Y	probably damaging	Het
Disc1	A	T	8: 125,088,275	T293S	probably benign	Het
Ercc1	T	C	7: 19,350,177	L113S	probably damaging	Het
Ermp1	G	T	19: 29,615,796	N765K	probably benign	Het
Fam83c	A	G	2: 155,834,820	I10T	probably benign	Het
Farp1	T	C	14: 121,275,596	V788A	probably benign	Het
Fbn1	C	G	2: 125,477,569	G92R	probably damaging	Het
Fsd1	T	A	17: 55,995,881	M328K	probably benign	Het
Fzd3	C	A	14: 65,202,813	S636I	probably benign	Het
Gapvd1	A	G	2: 34,704,318	S886P	probably damaging	Het
Gatad2b	T	A	3: 90,341,722	D22E	probably benign	Het
Gm10436	T	C	12: 88,177,080	Y329C	probably benign	Het
Gm30302	G	A	13: 49,786,072	R721C	probably benign	Het
Gm7168	A	T	17: 13,948,976	K202*	probably null	Het
Gmps	T	C	3: 63,980,269	I54T	probably damaging	Het
Gp6	A	G	7: 4,394,115	I107T	probably benign	Het
Gpat2	T	C	2: 127,431,347	V217A	possibly damaging	Het
Hdlbp	T	A	1: 93,417,195	I863F	probably damaging	Het
Hmcn1	A	C	1: 150,749,090	I1021M	possibly damaging	Het
Igkv6-14	C	T	6: 70,435,103	A66T	probably benign	Het
Ippk	C	T	13: 49,446,342	P226S		Het
Iqch	A	G	9: 63,454,573	I925T	probably benign	Het
Kcnt1	A	G	2: 25,901,211	E522G	possibly damaging	Het
Kremen2	C	G	17: 23,743,802	E103D	probably damaging	Het
Lig4	G	T	8: 9,973,485	N98K	probably benign	Het
Lrrc3b	T	G	14: 15,358,232	I125L	probably benign	Het
Lrrc4	T	C	6: 28,829,903	D148G	probably damaging	Het
Lrsam1	A	G	2: 32,947,889	Y218H	possibly damaging	Het
Mmrn2	G	A	14: 34,397,636	V191I	probably benign	Het
Nadk	T	A	4: 155,570,670		probably null	Het
Napb	C	A	2: 148,709,431	A61S	possibly damaging	Het
Ncan	T	A	8: 70,108,571	D582V	possibly damaging	Het
Ncl	T	C	1: 86,356,642	D244G	possibly damaging	Het
Nlrp14	A	G	7: 107,192,508	N800S	possibly damaging	Het
Nlrp9b	C	T	7: 20,019,335	T55M	probably benign	Het
Nobox	G	A	6: 43,306,017	T228I	probably benign	Het
Npffr1	C	A	10: 61,613,971	P8H	possibly damaging	Het
Nrg2	T	C	18: 36,021,103	Y587C	probably damaging	Het
Nsd1	T	G	13: 55,245,621	V448G	probably benign	Het
Olfr1016	A	G	2: 85,799,667	L201P	probably damaging	Het
Olfr228	T	G	2: 86,483,068	I225L	probably damaging	Het
Olfr263	T	A	13: 21,133,201	V142D	probably benign	Het
Olfr513	G	T	7: 108,755,231	C125F	probably damaging	Het
Peg10	T	TCCC	6: 4,756,451		probably benign	Het
Ppp5c	T	C	7: 17,009,007	T229A	probably benign	Het
Prr5	C	T	15: 84,693,792	R101C	probably damaging	Het
Psg22	T	C	7: 18,723,062	M290T	probably benign	Het
Pzp	C	T	6: 128,513,731		probably null	Het
Rapgef6	T	C	11: 54,625,958	V347A	probably benign	Het
Rttn	A	T	18: 89,010,916	H586L	probably damaging	Het
Sema3f	A	T	9: 107,688,076	I259K	possibly damaging	Het
Sgpp1	A	T	12: 75,716,600	L269Q	probably damaging	Het
Slc22a8	A	C	19: 8,605,539	I195L	probably benign	Het
Slc35a5	T	C	16: 45,142,188	T425A	unknown	Het
Stard13	G	A	5: 151,063,505	T180I	probably damaging	Het
Stard9	G	A	2: 120,704,430	G3723S	probably benign	Het
Svil	A	T	18: 5,062,385	T902S	probably damaging	Het
Tnfaip8l3	T	A	9: 54,027,137	R185W	probably damaging	Het
Ttn	A	T	2: 76,760,049	D21105E	probably benign	Het
Vmn2r125	A	G	4: 156,351,347	H340R	probably damaging	Het
Zfp946	T	C	17: 22,455,500	C412R	probably damaging	Het

Other mutations in Vmn2r109

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01313:Vmn2r109	APN	17	20550157	missense	probably damaging	1.00
IGL01383:Vmn2r109	APN	17	20541121	missense	possibly damaging	0.89
IGL01469:Vmn2r109	APN	17	20541409	missense	probably damaging	1.00
IGL01762:Vmn2r109	APN	17	20554392	missense	probably benign
IGL01864:Vmn2r109	APN	17	20541134	missense	probably benign	0.28
IGL02028:Vmn2r109	APN	17	20541080	missense	probably benign	0.28
IGL02074:Vmn2r109	APN	17	20554341	missense	probably benign	0.05
IGL02162:Vmn2r109	APN	17	20554160	missense	probably benign	0.01
IGL02474:Vmn2r109	APN	17	20540888	missense	probably benign
IGL02490:Vmn2r109	APN	17	20540984	missense	possibly damaging	0.78
IGL02604:Vmn2r109	APN	17	20540701	missense	probably damaging	1.00
IGL02669:Vmn2r109	APN	17	20554256	missense	possibly damaging	0.64
IGL02705:Vmn2r109	APN	17	20553800	missense	probably benign
IGL02745:Vmn2r109	APN	17	20541250	missense	probably damaging	0.99
PIT4142001:Vmn2r109	UTSW	17	20554577	critical splice acceptor site	probably null
R0389:Vmn2r109	UTSW	17	20541074	missense	probably damaging	1.00
R0470:Vmn2r109	UTSW	17	20552886	missense	probably benign	0.06
R0570:Vmn2r109	UTSW	17	20540675	missense	probably damaging	0.99
R0855:Vmn2r109	UTSW	17	20541408	nonsense	probably null
R0882:Vmn2r109	UTSW	17	20554580	splice site	probably benign
R1241:Vmn2r109	UTSW	17	20555241	missense	possibly damaging	0.86
R1587:Vmn2r109	UTSW	17	20540740	missense	probably damaging	1.00
R1931:Vmn2r109	UTSW	17	20553810	nonsense	probably null
R1957:Vmn2r109	UTSW	17	20564707	missense	probably benign	0.11
R1962:Vmn2r109	UTSW	17	20553923	missense	probably damaging	0.99
R2020:Vmn2r109	UTSW	17	20541186	nonsense	probably null
R2073:Vmn2r109	UTSW	17	20564712	missense	probably benign	0.00
R2436:Vmn2r109	UTSW	17	20554536	missense	probably damaging	0.99
R3123:Vmn2r109	UTSW	17	20540986	missense	probably damaging	1.00
R3839:Vmn2r109	UTSW	17	20554442	missense	probably damaging	1.00
R4019:Vmn2r109	UTSW	17	20553812	missense	probably benign
R4428:Vmn2r109	UTSW	17	20553024	missense	probably benign
R4584:Vmn2r109	UTSW	17	20554558	nonsense	probably null
R4652:Vmn2r109	UTSW	17	20541394	missense	probably damaging	1.00
R4708:Vmn2r109	UTSW	17	20541343	missense	probably damaging	0.97
R4823:Vmn2r109	UTSW	17	20553891	missense	probably damaging	1.00
R4831:Vmn2r109	UTSW	17	20541232	missense	probably benign	0.01
R4907:Vmn2r109	UTSW	17	20550086	missense	probably damaging	1.00
R5011:Vmn2r109	UTSW	17	20555189	missense	probably damaging	1.00
R5296:Vmn2r109	UTSW	17	20554341	missense	possibly damaging	0.90
R5600:Vmn2r109	UTSW	17	20540927	missense	probably damaging	1.00
R5602:Vmn2r109	UTSW	17	20540671	missense	possibly damaging	0.94
R5652:Vmn2r109	UTSW	17	20540519	makesense	probably null
R5702:Vmn2r109	UTSW	17	20554145	missense	probably benign	0.42
R5706:Vmn2r109	UTSW	17	20554305	missense	probably benign	0.16
R5714:Vmn2r109	UTSW	17	20552859	missense	probably damaging	1.00
R5832:Vmn2r109	UTSW	17	20541056	missense	probably benign	0.10
R6008:Vmn2r109	UTSW	17	20540719	missense	probably damaging	1.00
R6334:Vmn2r109	UTSW	17	20541178	missense	probably benign	0.18
R6377:Vmn2r109	UTSW	17	20564534	critical splice donor site	probably null
R6738:Vmn2r109	UTSW	17	20554523	missense	possibly damaging	0.52
R6857:Vmn2r109	UTSW	17	20540670	missense	probably benign	0.45
R6953:Vmn2r109	UTSW	17	20540711	missense	possibly damaging	0.95
R7108:Vmn2r109	UTSW	17	20564744	missense	probably benign	0.03
R7229:Vmn2r109	UTSW	17	20540963	missense	possibly damaging	0.80
R7238:Vmn2r109	UTSW	17	20541074	missense	probably damaging	1.00
R7244:Vmn2r109	UTSW	17	20540683	missense	possibly damaging	0.70
R7292:Vmn2r109	UTSW	17	20541438	missense	probably benign	0.05
R7354:Vmn2r109	UTSW	17	20540781	missense	probably damaging	1.00
R7357:Vmn2r109	UTSW	17	20541274	missense	probably damaging	1.00
R7522:Vmn2r109	UTSW	17	20554403	missense	probably benign	0.11
R7596:Vmn2r109	UTSW	17	20540680	missense	probably damaging	0.98
R7728:Vmn2r109	UTSW	17	20552855	missense	probably damaging	0.99
R7859:Vmn2r109	UTSW	17	20541174	missense	probably damaging	1.00
R7871:Vmn2r109	UTSW	17	20540520	missense	probably benign	0.08
R8153:Vmn2r109	UTSW	17	20564707	missense	probably benign	0.11
R8977:Vmn2r109	UTSW	17	20554269	missense	possibly damaging	0.96
R9687:Vmn2r109	UTSW	17	20555070	missense
Z1176:Vmn2r109	UTSW	17	20552994	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GACCTATGAGAGAATCAATGTCACC -3'
(R):5'- CAGGTTTGGCAGGAATTTCTGTAC -3'

Sequencing Primer
(F):5'- ATTTGTCTCATCCATATTTTCCCAG -3'
(R):5'- TGGCAGGAATTTCTGTACAATTC -3'

Posted On

2020-06-30