Mutagenetix > Incidental Mutation

Incidental Mutation 'R8114:Odr4'

631045

Institutional Source

Beutler Lab

Gene Symbol

Odr4

Ensembl Gene

ENSMUSG00000006010

Gene Name

odr4 GPCR localization factor homolog

Synonyms

BC003331, 1810053E15Rik

MMRRC Submission

067543-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.746) question?

Stock #

R8114 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

150237056-150268831 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 150264308 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Stop codon at position 67 (L67*)

Ref Sequence

ENSEMBL: ENSMUSP00000107544 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000006167] [ENSMUST00000094477] [ENSMUST00000097546] [ENSMUST00000097547] [ENSMUST00000111913] [ENSMUST00000119161] [ENSMUST00000124973]

AlphaFold

Q4PJX1

Predicted Effect

probably null
Transcript: ENSMUST00000006167
AA Change: L67*

SMART Domains

Protein: ENSMUSP00000006167
Gene: ENSMUSG00000006010
AA Change: L67*

Domain	Start	End	E-Value	Type
Pfam:ODR4-like	28	364	1.8e-107	PFAM
transmembrane domain	402	424	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000094477
AA Change: L67*

SMART Domains

Protein: ENSMUSP00000092050
Gene: ENSMUSG00000006010
AA Change: L67*

Domain	Start	End	E-Value	Type
Pfam:ODR4-like	28	383	2.8e-120	PFAM
transmembrane domain	424	446	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000097546
AA Change: L67*

SMART Domains

Protein: ENSMUSP00000095152
Gene: ENSMUSG00000006010
AA Change: L67*

Domain	Start	End	E-Value	Type
Pfam:ODR4-like	28	364	1.8e-107	PFAM
transmembrane domain	402	424	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000097547
AA Change: L67*

SMART Domains

Protein: ENSMUSP00000095153
Gene: ENSMUSG00000006010
AA Change: L67*

Domain	Start	End	E-Value	Type
Pfam:ODR4-like	28	209	1.2e-57	PFAM
Pfam:ODR4-like	206	354	3.1e-43	PFAM
transmembrane domain	392	414	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000111913
AA Change: L67*

SMART Domains

Protein: ENSMUSP00000107544
Gene: ENSMUSG00000006010
AA Change: L67*

Domain	Start	End	E-Value	Type
Pfam:ODR4-like	28	386	7.1e-113	PFAM
transmembrane domain	424	446	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000119161

SMART Domains

Protein: ENSMUSP00000112606
Gene: ENSMUSG00000006005

Domain	Start	End	E-Value	Type
coiled coil region	49	370	N/A	INTRINSIC
coiled coil region	423	515	N/A	INTRINSIC
low complexity region	518	534	N/A	INTRINSIC
coiled coil region	539	604	N/A	INTRINSIC
low complexity region	690	703	N/A	INTRINSIC
low complexity region	782	795	N/A	INTRINSIC
low complexity region	811	826	N/A	INTRINSIC
low complexity region	1003	1019	N/A	INTRINSIC
Pfam:TPR_MLP1_2	1036	1167	9.1e-33	PFAM
coiled coil region	1215	1421	N/A	INTRINSIC
coiled coil region	1473	1629	N/A	INTRINSIC
internal_repeat_3	1630	1691	1.48e-5	PROSPERO
low complexity region	1695	1717	N/A	INTRINSIC
low complexity region	1761	1777	N/A	INTRINSIC
internal_repeat_5	1814	1827	5.58e-5	PROSPERO
internal_repeat_3	1819	1881	1.48e-5	PROSPERO
internal_repeat_4	1875	1895	5.58e-5	PROSPERO
internal_repeat_1	1893	1919	2.03e-6	PROSPERO
low complexity region	1920	1933	N/A	INTRINSIC
low complexity region	1942	1981	N/A	INTRINSIC
low complexity region	1989	2014	N/A	INTRINSIC
internal_repeat_4	2017	2036	5.58e-5	PROSPERO
low complexity region	2059	2078	N/A	INTRINSIC
internal_repeat_2	2084	2135	3.95e-6	PROSPERO
internal_repeat_5	2127	2140	5.58e-5	PROSPERO
internal_repeat_1	2154	2179	2.03e-6	PROSPERO
internal_repeat_2	2156	2212	3.95e-6	PROSPERO
low complexity region	2239	2251	N/A	INTRINSIC
low complexity region	2263	2277	N/A	INTRINSIC
low complexity region	2292	2314	N/A	INTRINSIC
low complexity region	2346	2357	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000124973

SMART Domains

Protein: ENSMUSP00000117616
Gene: ENSMUSG00000006005

Domain	Start	End	E-Value	Type
low complexity region	3	14	N/A	INTRINSIC
low complexity region	24	77	N/A	INTRINSIC
coiled coil region	123	444	N/A	INTRINSIC
coiled coil region	497	589	N/A	INTRINSIC
low complexity region	592	608	N/A	INTRINSIC
coiled coil region	613	678	N/A	INTRINSIC
low complexity region	764	777	N/A	INTRINSIC
low complexity region	856	869	N/A	INTRINSIC
low complexity region	885	900	N/A	INTRINSIC
low complexity region	1077	1093	N/A	INTRINSIC
Pfam:TPR_MLP1_2	1112	1240	5.1e-37	PFAM
coiled coil region	1289	1495	N/A	INTRINSIC
low complexity region	1682	1698	N/A	INTRINSIC
internal_repeat_5	1703	1750	8.04e-5	PROSPERO
internal_repeat_3	1704	1765	1.07e-5	PROSPERO
low complexity region	1769	1791	N/A	INTRINSIC
low complexity region	1835	1851	N/A	INTRINSIC
internal_repeat_5	1857	1900	8.04e-5	PROSPERO
internal_repeat_6	1887	1911	8.04e-5	PROSPERO
internal_repeat_3	1893	1955	1.07e-5	PROSPERO
internal_repeat_4	1949	1969	4.1e-5	PROSPERO
internal_repeat_1	1967	1993	1.42e-6	PROSPERO
low complexity region	1994	2007	N/A	INTRINSIC
low complexity region	2016	2055	N/A	INTRINSIC
low complexity region	2063	2088	N/A	INTRINSIC
internal_repeat_4	2091	2110	4.1e-5	PROSPERO
internal_repeat_6	2108	2132	8.04e-5	PROSPERO
low complexity region	2133	2152	N/A	INTRINSIC
internal_repeat_2	2158	2209	2.78e-6	PROSPERO
internal_repeat_1	2228	2253	1.42e-6	PROSPERO
internal_repeat_2	2230	2286	2.78e-6	PROSPERO
low complexity region	2313	2325	N/A	INTRINSIC
low complexity region	2337	2351	N/A	INTRINSIC
low complexity region	2366	2388	N/A	INTRINSIC
low complexity region	2420	2431	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150615

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000140112

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000148389

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000131701

Meta Mutation Damage Score

0.9713

Coding Region Coverage

1x: 99.8%
3x: 99.5%
10x: 98.5%
20x: 94.7%

Validation Efficiency

100% (64/64)

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A630010A05Rik	G	T	16: 14,407,091 (GRCm39)	E7*	probably null	Het
Abca7	T	C	10: 79,844,874 (GRCm39)	I1532T	probably damaging	Het
Adam12	T	C	7: 133,569,617 (GRCm39)	D286G	probably damaging	Het
Ahnak2	T	C	12: 112,741,163 (GRCm39)	T970A	probably benign	Het
Apcdd1	A	G	18: 63,083,127 (GRCm39)	N319S	probably damaging	Het
Arih1	A	G	9: 59,303,836 (GRCm39)	M423T	probably benign	Het
Ccdc110	A	T	8: 46,396,140 (GRCm39)	Q677L	probably damaging	Het
Ccdc121rt3	C	A	5: 112,503,563 (GRCm39)	R47L	probably benign	Het
Ccdc171	A	G	4: 83,614,537 (GRCm39)	N1046S	probably damaging	Het
Ccdc63	G	T	5: 122,251,244 (GRCm39)	Q389K	possibly damaging	Het
Cd274	T	C	19: 29,361,528 (GRCm39)	S279P	probably damaging	Het
Cdcp2	A	T	4: 106,962,555 (GRCm39)	I243F	probably damaging	Het
Cfap298	T	C	16: 90,731,545 (GRCm39)	T22A	probably benign	Het
Cul2	A	T	18: 3,426,164 (GRCm39)	K414*	probably null	Het
Daglb	A	G	5: 143,464,218 (GRCm39)	H243R	probably benign	Het
Dnah5	A	T	15: 28,240,122 (GRCm39)	Y493F	probably benign	Het
Dsc2	C	T	18: 20,165,331 (GRCm39)	G881R	possibly damaging	Het
Eea1	G	T	10: 95,830,851 (GRCm39)	E171*	probably null	Het
Eml6	C	T	11: 29,704,910 (GRCm39)	G1545R	probably damaging	Het
Epg5	A	G	18: 78,073,365 (GRCm39)	I2463V	probably benign	Het
Fgl1	A	G	8: 41,644,620 (GRCm39)	Y295H	probably damaging	Het
Frrs1	A	G	3: 116,675,425 (GRCm39)	T118A	probably damaging	Het
Gdi2	T	C	13: 3,598,906 (GRCm39)	V30A	probably damaging	Het
Ints4	A	G	7: 97,165,732 (GRCm39)		probably null	Het
Iqsec3	A	G	6: 121,390,458 (GRCm39)	S338P	unknown	Het
Klhl3	C	T	13: 58,161,677 (GRCm39)	V473M	possibly damaging	Het
Ldah	A	G	12: 8,334,039 (GRCm39)	E310G	probably damaging	Het
Mtnr1b	A	G	9: 15,785,563 (GRCm39)	V65A	probably damaging	Het
Myo7b	G	T	18: 32,098,677 (GRCm39)	T1735K	probably damaging	Het
Neb	A	T	2: 52,215,734 (GRCm39)	V191D	possibly damaging	Het
Oas1e	T	C	5: 120,924,708 (GRCm39)	T377A	unknown	Het
Or10al6	T	C	17: 38,082,880 (GRCm39)	F112S	possibly damaging	Het
Or10v9	G	A	19: 11,832,466 (GRCm39)	P284S	probably damaging	Het
Or8k53	A	T	2: 86,177,530 (GRCm39)	H193Q	probably benign	Het
Platr25	T	C	13: 62,821,738 (GRCm39)	S70G	possibly damaging	Het
Plcl2	T	C	17: 50,994,815 (GRCm39)	S1095P	probably damaging	Het
Polq	A	G	16: 36,862,577 (GRCm39)	K637E	possibly damaging	Het
Rbm47	T	C	5: 66,184,196 (GRCm39)	I136V	probably benign	Het
Serpinb3c	A	C	1: 107,204,034 (GRCm39)	N59K	probably benign	Het
Shcbp1	T	A	8: 4,817,930 (GRCm39)	N122Y	probably damaging	Het
Sim2	T	C	16: 93,923,503 (GRCm39)	V347A	probably benign	Het
Slc22a30	A	T	19: 8,381,904 (GRCm39)	Y122*	probably null	Het
Slc34a2	G	A	5: 53,225,701 (GRCm39)	S450N	probably benign	Het
Snx17	A	T	5: 31,355,046 (GRCm39)	M354L	probably benign	Het
Stard9	G	A	2: 120,534,911 (GRCm39)	G3723S	probably benign	Het
Surf6	G	A	2: 26,782,380 (GRCm39)	Q316*	probably null	Het
Tiprl	A	G	1: 165,055,991 (GRCm39)	S44P	probably benign	Het
Tmem39a	T	G	16: 38,411,359 (GRCm39)	L438R	probably damaging	Het
Tmem39a	C	A	16: 38,411,358 (GRCm39)	L438M	probably damaging	Het
Tns2	G	C	15: 102,019,825 (GRCm39)	V572L	probably benign	Het
Trdn	T	C	10: 32,959,624 (GRCm39)		probably benign	Het
Trpc7	T	C	13: 56,952,411 (GRCm39)	I587V	probably benign	Het
Usp47	A	G	7: 111,692,394 (GRCm39)	D952G	probably damaging	Het
Vmn2r58	A	T	7: 41,511,392 (GRCm39)	N470K	probably damaging	Het
Vmn2r67	A	T	7: 84,805,097 (GRCm39)	I5N	probably benign	Het
Wdfy4	G	A	14: 32,826,072 (GRCm39)	P1193L		Het
Ythdc2	A	G	18: 45,010,807 (GRCm39)	D1272G	probably benign	Het
Zfp959	A	G	17: 56,205,496 (GRCm39)	Y511C	probably benign	Het

Other mutations in Odr4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01876:Odr4	APN	1	150,258,089 (GRCm39)	missense	probably benign	0.10
IGL02189:Odr4	APN	1	150,247,784 (GRCm39)	missense	possibly damaging	0.94
IGL02257:Odr4	APN	1	150,262,155 (GRCm39)	missense	probably damaging	0.99
IGL02485:Odr4	APN	1	150,239,240 (GRCm39)	critical splice donor site	probably null
IGL02585:Odr4	APN	1	150,239,272 (GRCm39)	missense	probably damaging	0.96
IGL02712:Odr4	APN	1	150,262,107 (GRCm39)	critical splice donor site	probably null
IGL02902:Odr4	APN	1	150,260,179 (GRCm39)	critical splice donor site	probably null
IGL03014:Odr4	APN	1	150,258,804 (GRCm39)	splice site	probably benign
IGL03124:Odr4	APN	1	150,262,176 (GRCm39)	missense	probably benign	0.00
IGL03181:Odr4	APN	1	150,239,290 (GRCm39)	missense	probably benign	0.06
IGL03344:Odr4	APN	1	150,239,295 (GRCm39)	missense	probably damaging	0.99
R1170:Odr4	UTSW	1	150,262,142 (GRCm39)	missense	probably benign	0.00
R1796:Odr4	UTSW	1	150,251,305 (GRCm39)	missense	probably benign
R1902:Odr4	UTSW	1	150,264,360 (GRCm39)	splice site	probably null
R2149:Odr4	UTSW	1	150,264,310 (GRCm39)	missense	probably benign	0.05
R2155:Odr4	UTSW	1	150,258,086 (GRCm39)	missense	possibly damaging	0.68
R2375:Odr4	UTSW	1	150,265,985 (GRCm39)	critical splice donor site	probably null
R3786:Odr4	UTSW	1	150,260,282 (GRCm39)	missense	probably benign	0.21
R3948:Odr4	UTSW	1	150,264,308 (GRCm39)	nonsense	probably null
R4589:Odr4	UTSW	1	150,260,238 (GRCm39)	missense	probably benign	0.11
R4590:Odr4	UTSW	1	150,262,103 (GRCm39)	splice site	probably null
R4815:Odr4	UTSW	1	150,250,597 (GRCm39)	missense	probably damaging	0.99
R5196:Odr4	UTSW	1	150,258,140 (GRCm39)	missense	probably damaging	1.00
R5437:Odr4	UTSW	1	150,239,269 (GRCm39)	missense	probably benign	0.01
R5549:Odr4	UTSW	1	150,247,909 (GRCm39)	missense	possibly damaging	0.86
R5677:Odr4	UTSW	1	150,250,588 (GRCm39)	missense	probably damaging	1.00
R5896:Odr4	UTSW	1	150,256,111 (GRCm39)	missense	probably benign	0.10
R6472:Odr4	UTSW	1	150,257,273 (GRCm39)	missense	probably benign	0.15
R7108:Odr4	UTSW	1	150,258,041 (GRCm39)	missense	probably benign	0.01
R7402:Odr4	UTSW	1	150,262,107 (GRCm39)	critical splice donor site	probably null
R7662:Odr4	UTSW	1	150,258,045 (GRCm39)	missense	probably benign
R7767:Odr4	UTSW	1	150,247,788 (GRCm39)	missense	probably benign	0.00
R7810:Odr4	UTSW	1	150,268,659 (GRCm39)	utr 5 prime	probably benign
R7916:Odr4	UTSW	1	150,260,249 (GRCm39)	missense	probably benign	0.01
R8120:Odr4	UTSW	1	150,260,177 (GRCm39)	splice site	probably null
R8435:Odr4	UTSW	1	150,258,020 (GRCm39)	missense	possibly damaging	0.90
R9397:Odr4	UTSW	1	150,238,616 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACCATTCAATATTCAGAGCCTTCTC -3'
(R):5'- GCTGTTCACTAAAATCAGTCCC -3'

Sequencing Primer
(F):5'- AGCCTTCTCTAAAACTGTTTAAGTAC -3'
(R):5'- CAGTCCCCAAGAATATAGTTTTCATC -3'

Posted On

2020-06-30