Mutagenetix > Incidental Mutation

Incidental Mutation 'R8115:Wdfy4'

631154

Institutional Source

Beutler Lab

Gene Symbol

Wdfy4

Ensembl Gene

ENSMUSG00000051506

Gene Name

WD repeat and FYVE domain containing 4

Synonyms

MMRRC Submission

067544-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8115 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

32681504-32907465 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 32826072 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Leucine at position 1193 (P1193L)

Ref Sequence

ENSEMBL: ENSMUSP00000117068 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061753] [ENSMUST00000130509]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000061753
AA Change: P1148L

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000057556
Gene: ENSMUSG00000051506
AA Change: P1148L

Domain	Start	End	E-Value	Type
low complexity region	69	77	N/A	INTRINSIC
low complexity region	204	222	N/A	INTRINSIC
low complexity region	508	522	N/A	INTRINSIC
low complexity region	618	632	N/A	INTRINSIC
low complexity region	644	661	N/A	INTRINSIC
low complexity region	1585	1604	N/A	INTRINSIC
low complexity region	1899	1909	N/A	INTRINSIC
Pfam:PH_BEACH	2237	2348	1.2e-9	PFAM
Beach	2378	2660	3.69e-196	SMART
WD40	2761	2801	1.98e1	SMART
WD40	2811	2850	5.18e-7	SMART
WD40	2853	2891	9.94e-1	SMART
WD40	2893	2940	3.17e-2	SMART
WD40	2986	3021	3.31e0	SMART

Predicted Effect

SMART Domains

Protein: ENSMUSP00000117068
Gene: ENSMUSG00000051506
AA Change: P1193L

Domain	Start	End	E-Value	Type
low complexity region	69	77	N/A	INTRINSIC
low complexity region	204	222	N/A	INTRINSIC
low complexity region	508	522	N/A	INTRINSIC
low complexity region	618	632	N/A	INTRINSIC
low complexity region	644	661	N/A	INTRINSIC
low complexity region	1596	1615	N/A	INTRINSIC
low complexity region	1795	1819	N/A	INTRINSIC
low complexity region	2019	2029	N/A	INTRINSIC
Pfam:PH_BEACH	2362	2473	1.2e-9	PFAM
Beach	2503	2785	3.69e-196	SMART
WD40	2886	2926	1.98e1	SMART
WD40	2936	2975	5.18e-7	SMART
WD40	2978	3016	9.94e-1	SMART
WD40	3018	3065	3.17e-2	SMART
WD40	3111	3146	3.31e0	SMART

Meta Mutation Damage Score

0.6747

Coding Region Coverage

1x: 99.7%
3x: 99.5%
10x: 98.4%
20x: 94.2%

Validation Efficiency

100% (68/68)

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A1bg	T	G	15: 60,791,996 (GRCm39)	I211L	probably benign	Het
Abhd16b	G	A	2: 181,135,527 (GRCm39)	R143H	possibly damaging	Het
Abt1	T	C	13: 23,606,402 (GRCm39)	E184G	probably damaging	Het
Aqp1	AGG	AGGG	6: 55,322,498 (GRCm39)		probably null	Het
Asxl3	G	A	18: 22,650,642 (GRCm39)	R877Q	probably damaging	Het
B3galt1	A	G	2: 67,948,320 (GRCm39)	T12A	possibly damaging	Het
Bcat1	G	A	6: 144,955,819 (GRCm39)	P354L	probably damaging	Het
C9orf72	T	C	4: 35,218,763 (GRCm39)	Y32C		Het
Cad	T	C	5: 31,218,271 (GRCm39)	F452L	possibly damaging	Het
Cadps2	T	A	6: 23,328,897 (GRCm39)	I973F	probably benign	Het
Cenpq	T	C	17: 41,243,720 (GRCm39)	N43D	probably damaging	Het
Chd9	G	T	8: 91,762,960 (GRCm39)	V2262L	probably damaging	Het
Cttnbp2nl	G	T	3: 104,913,402 (GRCm39)	Q161K	probably damaging	Het
D630003M21Rik	A	T	2: 158,058,510 (GRCm39)	H463Q	probably benign	Het
Defb1	C	T	8: 22,284,500 (GRCm39)	H40Y	probably benign	Het
Dmrtb1	A	T	4: 107,534,256 (GRCm39)	D186E	probably benign	Het
Dsc2	C	T	18: 20,165,331 (GRCm39)	G881R	possibly damaging	Het
Efr3a	T	A	15: 65,738,644 (GRCm39)	F758I	probably damaging	Het
Fap	A	G	2: 62,349,385 (GRCm39)	I501T	probably benign	Het
Galr3	C	T	15: 78,927,524 (GRCm39)	R322*	probably null	Het
Hrnr	C	A	3: 93,231,039 (GRCm39)	R426S	unknown	Het
Hsp90ab1	A	T	17: 45,880,201 (GRCm39)	M476K	possibly damaging	Het
Igsf3	G	T	3: 101,362,595 (GRCm39)	R872L	probably benign	Het
Ippk	C	T	13: 49,599,818 (GRCm39)	P226S		Het
Iqsec3	T	G	6: 121,449,989 (GRCm39)	R178S	unknown	Het
Itgbl1	T	C	14: 124,094,955 (GRCm39)	C327R	probably damaging	Het
Itsn2	A	T	12: 4,723,602 (GRCm39)	Q1179L	possibly damaging	Het
Kcnb2	T	C	1: 15,781,851 (GRCm39)	*908R	probably null	Het
Kdm5b	G	T	1: 134,547,411 (GRCm39)	W1020L	possibly damaging	Het
Kpna3	C	A	14: 61,608,367 (GRCm39)	V364L	probably damaging	Het
Lsm14a	T	C	7: 34,074,662 (GRCm39)	I93V	probably benign	Het
Mast2	A	G	4: 116,292,644 (GRCm39)	S109P	probably benign	Het
Muc4	G	A	16: 32,575,678 (GRCm39)	S1726N	unknown	Het
Myo7a	T	C	7: 97,715,653 (GRCm39)	D1477G	probably damaging	Het
Nostrin	G	A	2: 69,011,264 (GRCm39)		probably null	Het
Oasl1	T	A	5: 115,074,996 (GRCm39)	V352E	probably damaging	Het
Or9a2	T	A	6: 41,748,472 (GRCm39)	T254S	probably benign	Het
Pafah1b1	A	G	11: 74,575,319 (GRCm39)	V195A	probably damaging	Het
Pcdhb4	G	A	18: 37,442,453 (GRCm39)	V588M	probably damaging	Het
Pcsk5	A	G	19: 17,487,530 (GRCm39)		probably null	Het
Peg10	A	G	6: 4,756,707 (GRCm39)	I428V	unknown	Het
Pmfbp1	G	A	8: 110,263,669 (GRCm39)	V824M	probably damaging	Het
Prkag3	T	C	1: 74,787,118 (GRCm39)	R47G	possibly damaging	Het
Prl8a1	T	A	13: 27,758,028 (GRCm39)	H227L	probably benign	Het
Prune2	A	G	19: 17,101,288 (GRCm39)	D2264G	probably benign	Het
Psg28	A	G	7: 18,164,311 (GRCm39)	Y134H	probably benign	Het
Rab3gap2	T	A	1: 184,999,447 (GRCm39)	Y1019N	possibly damaging	Het
Rbm15	A	T	3: 107,238,966 (GRCm39)	F477L	probably damaging	Het
Ric1	A	T	19: 29,563,973 (GRCm39)	N576Y	probably damaging	Het
S1pr1	T	A	3: 115,506,298 (GRCm39)	T99S	probably benign	Het
Serpinb9b	G	T	13: 33,219,531 (GRCm39)	V153F	probably null	Het
Sh2d3c	A	G	2: 32,615,276 (GRCm39)	E122G	probably benign	Het
Slc6a11	T	A	6: 114,108,442 (GRCm39)	W69R	probably damaging	Het
Slco6c1	T	A	1: 97,000,686 (GRCm39)	I539F	probably damaging	Het
Smgc	G	T	15: 91,733,322 (GRCm39)		probably null	Het
Spata17	T	C	1: 186,849,653 (GRCm39)	Y194C	probably damaging	Het
Tap1	A	T	17: 34,412,293 (GRCm39)		probably null	Het
Topbp1	T	C	9: 103,197,740 (GRCm39)	S440P	probably benign	Het
Trpc6	T	C	9: 8,609,982 (GRCm39)	L150P	probably damaging	Het
Ttll4	C	T	1: 74,726,489 (GRCm39)	Q696*	probably null	Het
Ubash3b	C	T	9: 40,937,624 (GRCm39)	E447K	probably damaging	Het
Unc5cl	T	C	17: 48,774,438 (GRCm39)	S477P	possibly damaging	Het
Unc80	A	G	1: 66,688,072 (GRCm39)	T2357A	probably benign	Het
Vmn1r58	A	T	7: 5,413,341 (GRCm39)	S296R	probably benign	Het
Vmn2r85	G	T	10: 130,261,820 (GRCm39)	N172K	probably benign	Het
Yipf7	T	A	5: 69,684,570 (GRCm39)	T82S	probably benign	Het
Zap70	A	G	1: 36,820,287 (GRCm39)	S523G	probably damaging	Het
Zfp354a	A	G	11: 50,960,490 (GRCm39)	T233A	probably benign	Het

Other mutations in Wdfy4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00231:Wdfy4	APN	14	32,824,496 (GRCm39)	missense	possibly damaging	0.93
IGL01116:Wdfy4	APN	14	32,681,934 (GRCm39)	missense	probably damaging	1.00
IGL01449:Wdfy4	APN	14	32,825,994 (GRCm39)	missense	probably damaging	0.99
IGL01567:Wdfy4	APN	14	32,873,618 (GRCm39)	missense	probably benign	0.01
IGL01700:Wdfy4	APN	14	32,742,195 (GRCm39)	splice site	probably benign
IGL01931:Wdfy4	APN	14	32,877,710 (GRCm39)	missense	probably damaging	1.00
IGL01981:Wdfy4	APN	14	32,855,673 (GRCm39)	missense	probably damaging	1.00
IGL01988:Wdfy4	APN	14	32,798,437 (GRCm39)	missense	possibly damaging	0.75
IGL02026:Wdfy4	APN	14	32,815,257 (GRCm39)	missense	probably damaging	1.00
IGL02066:Wdfy4	APN	14	32,871,523 (GRCm39)	missense	probably benign
IGL02468:Wdfy4	APN	14	32,688,389 (GRCm39)	missense	probably benign	0.01
IGL02512:Wdfy4	APN	14	32,764,448 (GRCm39)	missense	probably benign	0.01
IGL02597:Wdfy4	APN	14	32,812,818 (GRCm39)	nonsense	probably null
IGL02752:Wdfy4	APN	14	32,798,283 (GRCm39)	missense	probably damaging	1.00
IGL02792:Wdfy4	APN	14	32,817,262 (GRCm39)	missense	probably benign	0.01
IGL02826:Wdfy4	APN	14	32,693,707 (GRCm39)	missense	possibly damaging	0.47
IGL02903:Wdfy4	APN	14	32,831,607 (GRCm39)	missense	probably damaging	1.00
IGL02955:Wdfy4	APN	14	32,798,241 (GRCm39)	missense	probably damaging	1.00
IGL03031:Wdfy4	APN	14	32,862,608 (GRCm39)	missense	probably damaging	1.00
IGL03102:Wdfy4	APN	14	32,688,392 (GRCm39)	missense	probably damaging	1.00
IGL03123:Wdfy4	APN	14	32,884,827 (GRCm39)	missense	probably benign	0.01
IGL03198:Wdfy4	APN	14	32,847,844 (GRCm39)	missense	probably damaging	1.00
IGL03250:Wdfy4	APN	14	32,699,124 (GRCm39)	missense	probably damaging	0.99
IGL03277:Wdfy4	APN	14	32,790,861 (GRCm39)	missense	probably benign	0.01
IGL03398:Wdfy4	APN	14	32,769,247 (GRCm39)	missense	probably benign	0.14
dodgers	UTSW	14	32,699,063 (GRCm39)	nonsense	probably null
Dollar	UTSW	14	32,742,268 (GRCm39)	missense	probably damaging	1.00
Giants	UTSW	14	32,792,575 (GRCm39)	nonsense	probably null
gigantea	UTSW	14	32,696,111 (GRCm39)	critical splice donor site	probably null
kings_canyon	UTSW	14	32,831,476 (GRCm39)	nonsense	probably null
moro	UTSW	14	32,686,583 (GRCm39)	splice site	probably null
popped	UTSW	14	32,688,356 (GRCm39)	missense	probably damaging	0.99
sequoia	UTSW	14	32,822,860 (GRCm39)	critical splice donor site	probably null
Sherman	UTSW	14	32,817,908 (GRCm39)	missense	possibly damaging	0.89
stretched	UTSW	14	32,795,492 (GRCm39)	nonsense	probably null
watchtower	UTSW	14	32,805,596 (GRCm39)	critical splice donor site	probably null
R0014:Wdfy4	UTSW	14	32,829,130 (GRCm39)	missense	possibly damaging	0.72
R0067:Wdfy4	UTSW	14	32,884,708 (GRCm39)	missense	probably null	1.00
R0085:Wdfy4	UTSW	14	32,800,200 (GRCm39)	missense	possibly damaging	0.81
R0277:Wdfy4	UTSW	14	32,805,742 (GRCm39)	missense	possibly damaging	0.83
R0436:Wdfy4	UTSW	14	32,805,769 (GRCm39)	splice site	probably benign
R0496:Wdfy4	UTSW	14	32,862,695 (GRCm39)	splice site	probably benign
R0514:Wdfy4	UTSW	14	32,802,732 (GRCm39)	missense	probably benign	0.22
R0548:Wdfy4	UTSW	14	32,764,578 (GRCm39)	missense	probably benign
R0590:Wdfy4	UTSW	14	32,763,131 (GRCm39)	missense	probably benign	0.09
R0647:Wdfy4	UTSW	14	32,831,656 (GRCm39)	missense	possibly damaging	0.96
R0766:Wdfy4	UTSW	14	32,862,569 (GRCm39)	missense	probably damaging	1.00
R0981:Wdfy4	UTSW	14	32,869,049 (GRCm39)	missense	probably benign	0.03
R1024:Wdfy4	UTSW	14	32,801,923 (GRCm39)	missense	possibly damaging	0.81
R1113:Wdfy4	UTSW	14	32,693,695 (GRCm39)	missense	possibly damaging	0.47
R1252:Wdfy4	UTSW	14	32,693,729 (GRCm39)	splice site	probably null
R1415:Wdfy4	UTSW	14	32,763,137 (GRCm39)	missense	possibly damaging	0.60
R1475:Wdfy4	UTSW	14	32,830,645 (GRCm39)	missense	probably benign	0.14
R1483:Wdfy4	UTSW	14	32,822,923 (GRCm39)	missense	probably benign	0.41
R1490:Wdfy4	UTSW	14	32,874,495 (GRCm39)	critical splice donor site	probably null
R1512:Wdfy4	UTSW	14	32,682,765 (GRCm39)	missense	probably damaging	0.98
R1615:Wdfy4	UTSW	14	32,764,469 (GRCm39)	missense	probably damaging	1.00
R1628:Wdfy4	UTSW	14	32,681,918 (GRCm39)	missense	probably damaging	1.00
R1643:Wdfy4	UTSW	14	32,795,542 (GRCm39)	critical splice acceptor site	probably null
R1729:Wdfy4	UTSW	14	32,817,962 (GRCm39)	missense	possibly damaging	0.85
R1859:Wdfy4	UTSW	14	32,825,940 (GRCm39)	missense	probably damaging	0.99
R1933:Wdfy4	UTSW	14	32,855,301 (GRCm39)	missense	probably benign	0.08
R1957:Wdfy4	UTSW	14	32,693,641 (GRCm39)	missense	probably damaging	1.00
R1968:Wdfy4	UTSW	14	32,828,001 (GRCm39)	missense	possibly damaging	0.95
R2032:Wdfy4	UTSW	14	32,868,946 (GRCm39)	missense	probably benign	0.11
R2241:Wdfy4	UTSW	14	32,795,468 (GRCm39)	missense	possibly damaging	0.81
R2391:Wdfy4	UTSW	14	32,884,764 (GRCm39)	missense	possibly damaging	0.92
R2888:Wdfy4	UTSW	14	32,831,476 (GRCm39)	nonsense	probably null
R2889:Wdfy4	UTSW	14	32,831,476 (GRCm39)	nonsense	probably null
R3114:Wdfy4	UTSW	14	32,811,860 (GRCm39)	missense	probably damaging	0.97
R3757:Wdfy4	UTSW	14	32,745,331 (GRCm39)	missense	probably benign	0.17
R3758:Wdfy4	UTSW	14	32,745,331 (GRCm39)	missense	probably benign	0.17
R3797:Wdfy4	UTSW	14	32,862,602 (GRCm39)	missense	probably damaging	1.00
R3890:Wdfy4	UTSW	14	32,769,237 (GRCm39)	missense	probably damaging	1.00
R3892:Wdfy4	UTSW	14	32,769,237 (GRCm39)	missense	probably damaging	1.00
R3945:Wdfy4	UTSW	14	32,688,352 (GRCm39)	missense	probably damaging	0.99
R4011:Wdfy4	UTSW	14	32,824,637 (GRCm39)	splice site	probably benign
R4091:Wdfy4	UTSW	14	32,847,837 (GRCm39)	missense	possibly damaging	0.93
R4449:Wdfy4	UTSW	14	32,818,040 (GRCm39)	missense	probably damaging	1.00
R4585:Wdfy4	UTSW	14	32,809,912 (GRCm39)	missense	possibly damaging	0.89
R4628:Wdfy4	UTSW	14	32,824,515 (GRCm39)	missense	probably damaging	0.97
R4629:Wdfy4	UTSW	14	32,824,515 (GRCm39)	missense	probably damaging	0.97
R4655:Wdfy4	UTSW	14	32,711,893 (GRCm39)	missense	probably damaging	0.98
R4689:Wdfy4	UTSW	14	32,831,505 (GRCm39)	missense	possibly damaging	0.88
R4718:Wdfy4	UTSW	14	32,867,273 (GRCm39)	missense	probably benign	0.03
R4862:Wdfy4	UTSW	14	32,822,860 (GRCm39)	critical splice donor site	probably null
R4884:Wdfy4	UTSW	14	32,710,852 (GRCm39)	nonsense	probably null
R4894:Wdfy4	UTSW	14	32,877,717 (GRCm39)	missense	probably benign	0.03
R4929:Wdfy4	UTSW	14	32,769,213 (GRCm39)	missense	possibly damaging	0.90
R4932:Wdfy4	UTSW	14	32,750,970 (GRCm39)	missense	probably damaging	1.00
R5014:Wdfy4	UTSW	14	32,822,897 (GRCm39)	missense	probably benign	0.02
R5020:Wdfy4	UTSW	14	32,801,892 (GRCm39)	missense	probably damaging	1.00
R5049:Wdfy4	UTSW	14	32,874,627 (GRCm39)	missense	possibly damaging	0.78
R5276:Wdfy4	UTSW	14	32,769,232 (GRCm39)	missense	probably damaging	1.00
R5318:Wdfy4	UTSW	14	32,800,300 (GRCm39)	missense	possibly damaging	0.95
R5338:Wdfy4	UTSW	14	32,812,823 (GRCm39)	missense	probably damaging	1.00
R5349:Wdfy4	UTSW	14	32,710,856 (GRCm39)	missense	probably damaging	1.00
R5411:Wdfy4	UTSW	14	32,681,959 (GRCm39)	missense	probably damaging	1.00
R5435:Wdfy4	UTSW	14	32,742,268 (GRCm39)	missense	probably damaging	1.00
R5463:Wdfy4	UTSW	14	32,873,689 (GRCm39)	missense	probably benign	0.17
R5591:Wdfy4	UTSW	14	32,829,087 (GRCm39)	missense	probably benign	0.09
R5598:Wdfy4	UTSW	14	32,855,454 (GRCm39)	missense	probably damaging	1.00
R5654:Wdfy4	UTSW	14	32,829,575 (GRCm39)	splice site	probably null
R5890:Wdfy4	UTSW	14	32,824,534 (GRCm39)	missense	possibly damaging	0.91
R5894:Wdfy4	UTSW	14	32,855,317 (GRCm39)	missense	possibly damaging	0.86
R5964:Wdfy4	UTSW	14	32,827,968 (GRCm39)	missense	probably damaging	1.00
R6036:Wdfy4	UTSW	14	32,868,947 (GRCm39)	missense	probably damaging	0.97
R6036:Wdfy4	UTSW	14	32,868,947 (GRCm39)	missense	probably damaging	0.97
R6074:Wdfy4	UTSW	14	32,805,596 (GRCm39)	critical splice donor site	probably null
R6135:Wdfy4	UTSW	14	32,693,668 (GRCm39)	missense	probably damaging	0.99
R6276:Wdfy4	UTSW	14	32,831,482 (GRCm39)	missense	possibly damaging	0.54
R6357:Wdfy4	UTSW	14	32,823,006 (GRCm39)	nonsense	probably null
R6370:Wdfy4	UTSW	14	32,790,807 (GRCm39)	missense	probably benign	0.16
R6390:Wdfy4	UTSW	14	32,826,051 (GRCm39)	missense	probably damaging	0.99
R6413:Wdfy4	UTSW	14	32,689,604 (GRCm39)	missense	probably damaging	1.00
R6450:Wdfy4	UTSW	14	32,830,649 (GRCm39)	missense	probably damaging	1.00
R6522:Wdfy4	UTSW	14	32,868,901 (GRCm39)	missense	probably damaging	0.98
R6657:Wdfy4	UTSW	14	32,769,208 (GRCm39)	missense	possibly damaging	0.70
R6761:Wdfy4	UTSW	14	32,817,908 (GRCm39)	missense	possibly damaging	0.89
R6763:Wdfy4	UTSW	14	32,764,469 (GRCm39)	missense	probably damaging	1.00
R6952:Wdfy4	UTSW	14	32,681,923 (GRCm39)	missense	probably damaging	1.00
R6985:Wdfy4	UTSW	14	32,821,074 (GRCm39)	missense	possibly damaging	0.68
R7024:Wdfy4	UTSW	14	32,686,583 (GRCm39)	splice site	probably null
R7101:Wdfy4	UTSW	14	32,682,777 (GRCm39)	missense
R7114:Wdfy4	UTSW	14	32,693,531 (GRCm39)	splice site	probably null
R7139:Wdfy4	UTSW	14	32,873,535 (GRCm39)	missense
R7255:Wdfy4	UTSW	14	32,696,239 (GRCm39)	missense
R7324:Wdfy4	UTSW	14	32,769,271 (GRCm39)	missense
R7379:Wdfy4	UTSW	14	32,873,566 (GRCm39)	missense
R7399:Wdfy4	UTSW	14	32,790,863 (GRCm39)	missense
R7408:Wdfy4	UTSW	14	32,800,264 (GRCm39)	missense
R7410:Wdfy4	UTSW	14	32,696,191 (GRCm39)	missense
R7411:Wdfy4	UTSW	14	32,828,088 (GRCm39)	missense
R7412:Wdfy4	UTSW	14	32,871,541 (GRCm39)	missense
R7445:Wdfy4	UTSW	14	32,792,575 (GRCm39)	nonsense	probably null
R7595:Wdfy4	UTSW	14	32,696,111 (GRCm39)	critical splice donor site	probably null
R7618:Wdfy4	UTSW	14	32,707,696 (GRCm39)	missense
R7622:Wdfy4	UTSW	14	32,800,231 (GRCm39)	missense
R7828:Wdfy4	UTSW	14	32,710,878 (GRCm39)	missense	possibly damaging	0.90
R7888:Wdfy4	UTSW	14	32,812,920 (GRCm39)	missense
R7946:Wdfy4	UTSW	14	32,792,705 (GRCm39)	missense
R7946:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R7986:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R7990:Wdfy4	UTSW	14	32,819,752 (GRCm39)	missense
R8001:Wdfy4	UTSW	14	32,695,492 (GRCm39)	critical splice donor site	probably null
R8010:Wdfy4	UTSW	14	32,693,584 (GRCm39)	missense
R8015:Wdfy4	UTSW	14	32,829,704 (GRCm39)	missense
R8032:Wdfy4	UTSW	14	32,751,043 (GRCm39)	nonsense	probably null
R8041:Wdfy4	UTSW	14	32,875,965 (GRCm39)	critical splice donor site	probably null
R8090:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8092:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8112:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8114:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8117:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8117:Wdfy4	UTSW	14	32,699,063 (GRCm39)	nonsense	probably null
R8118:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8140:Wdfy4	UTSW	14	32,864,317 (GRCm39)	missense
R8155:Wdfy4	UTSW	14	32,884,776 (GRCm39)	missense
R8163:Wdfy4	UTSW	14	32,873,545 (GRCm39)	missense
R8293:Wdfy4	UTSW	14	32,696,218 (GRCm39)	missense
R8325:Wdfy4	UTSW	14	32,689,444 (GRCm39)	missense
R8353:Wdfy4	UTSW	14	32,695,581 (GRCm39)	missense	probably benign
R8370:Wdfy4	UTSW	14	32,815,208 (GRCm39)	missense
R8437:Wdfy4	UTSW	14	32,798,332 (GRCm39)	missense
R8497:Wdfy4	UTSW	14	32,688,356 (GRCm39)	missense	probably damaging	0.99
R8545:Wdfy4	UTSW	14	32,800,258 (GRCm39)	missense	probably benign	0.01
R8671:Wdfy4	UTSW	14	32,693,722 (GRCm39)	splice site	probably benign
R8708:Wdfy4	UTSW	14	32,689,489 (GRCm39)	missense
R8747:Wdfy4	UTSW	14	32,874,611 (GRCm39)	missense
R8794:Wdfy4	UTSW	14	32,869,049 (GRCm39)	missense	probably benign	0.03
R8846:Wdfy4	UTSW	14	32,867,105 (GRCm39)	missense
R8880:Wdfy4	UTSW	14	32,795,492 (GRCm39)	nonsense	probably null
R9109:Wdfy4	UTSW	14	32,760,704 (GRCm39)	splice site	probably null
R9131:Wdfy4	UTSW	14	32,819,807 (GRCm39)	missense
R9309:Wdfy4	UTSW	14	32,817,313 (GRCm39)	missense
R9349:Wdfy4	UTSW	14	32,875,996 (GRCm39)	missense
R9451:Wdfy4	UTSW	14	32,855,518 (GRCm39)	missense
R9563:Wdfy4	UTSW	14	32,692,833 (GRCm39)	missense
R9587:Wdfy4	UTSW	14	32,769,230 (GRCm39)	nonsense	probably null
R9599:Wdfy4	UTSW	14	32,855,428 (GRCm39)	missense
R9670:Wdfy4	UTSW	14	32,769,219 (GRCm39)	missense
R9718:Wdfy4	UTSW	14	32,847,893 (GRCm39)	missense
R9742:Wdfy4	UTSW	14	32,809,987 (GRCm39)	missense
X0028:Wdfy4	UTSW	14	32,802,593 (GRCm39)	missense	probably benign
X0053:Wdfy4	UTSW	14	32,884,899 (GRCm39)	start codon destroyed	probably null	0.99
X0062:Wdfy4	UTSW	14	32,829,575 (GRCm39)	splice site	probably null
Z1177:Wdfy4	UTSW	14	32,809,942 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- GGCCGAGTTTGATAATAAGTGC -3'
(R):5'- CTGTTGCCTTCATAGGATCTGC -3'

Sequencing Primer
(F):5'- ATAAGTGCTAGAGTATCCGCTG -3'
(R):5'- AGGATCTGCTAATTAATCGTTTGCCC -3'

Posted On

2020-06-30