Mutagenetix > Incidental Mutation

Incidental Mutation 'R8118:Olfr998'

631315

Institutional Source

Beutler Lab

Gene Symbol

Olfr998

Ensembl Gene

ENSMUSG00000111454

Gene Name

olfactory receptor 998

Synonyms

GA_x6K02T2Q125-47069356-47070300, MOR175-5

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.210) question?

Stock #

R8118 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

85588948-85591893 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 85590988 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 149 (Y149*)

Ref Sequence

ENSEMBL: ENSMUSP00000150713 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000052307] [ENSMUST00000215083]

AlphaFold

Q8VF76

Predicted Effect

probably null
Transcript: ENSMUST00000052307
AA Change: Y149*

SMART Domains

Protein: ENSMUSP00000059970
Gene: ENSMUSG00000111454
AA Change: Y149*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	2.6e-54	PFAM
Pfam:7tm_1	41	308	9.5e-18	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000215083
AA Change: Y149*

Coding Region Coverage

1x: 99.8%
3x: 99.5%
10x: 98.2%
20x: 91.1%

Validation Efficiency

97% (68/70)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts1	T	C	16: 85,795,933	D792G	probably damaging	Het
Adcy7	A	G	8: 88,315,756	H417R	probably damaging	Het
Agr2	A	G	12: 35,996,107	D79G	probably benign	Het
Ankle1	T	C	8: 71,407,635	S286P	probably benign	Het
Arhgef11	T	C	3: 87,735,857	S1488P	probably damaging	Het
Atp6v0a2	T	C	5: 124,712,773	M421T	probably damaging	Het
Cdk17	C	A	10: 93,216,390	Q111K	possibly damaging	Het
Cobl	A	G	11: 12,254,834	S623P	probably benign	Het
Dgka	C	T	10: 128,722,449		probably null	Het
Dsc2	C	T	18: 20,032,274	G881R	possibly damaging	Het
Dsg2	A	G	18: 20,582,801	I267V	probably benign	Het
Exoc4	T	C	6: 33,971,918	Y899H	probably damaging	Het
Fat3	A	G	9: 15,960,104	F3664L	probably benign	Het
Fbxo47	C	T	11: 97,879,515	C17Y	probably benign	Het
Gm2888	G	T	14: 3,037,628	V207F	probably benign	Het
Gm7030	C	T	17: 36,127,690	V270M	probably damaging	Het
Gpr61	A	G	3: 108,150,572	S258P	probably damaging	Het
Hectd4	C	T	5: 121,286,376	H700Y	probably benign	Het
Hs3st2	C	T	7: 121,397,428	T154I	probably benign	Het
Inf2	A	T	12: 112,601,437	H167L	probably damaging	Het
Ints3	C	T	3: 90,400,299		probably null	Het
Ippk	C	T	13: 49,446,342	P226S		Het
Itgal	C	A	7: 127,311,245	Q509K	probably benign	Het
Klhl20	A	T	1: 161,098,401		probably null	Het
Krtap4-13	C	T	11: 99,809,398	C145Y	unknown	Het
Large1	A	G	8: 73,131,944	S99P	probably benign	Het
Lexm	T	A	4: 106,613,398	R192S	possibly damaging	Het
Lrba	A	T	3: 86,354,226	I1496L	probably benign	Het
Map2	A	T	1: 66,425,391	I1647F	probably damaging	Het
Map3k10	A	G	7: 27,673,417	V203A	possibly damaging	Het
Mcmdc2	T	A	1: 9,916,374	N166K	possibly damaging	Het
Mlxipl	T	G	5: 135,137,248	L828R	possibly damaging	Het
Mnat1	A	G	12: 73,219,090	I253V	probably benign	Het
Mtfp1	A	G	11: 4,093,910	S107P	probably damaging	Het
Nebl	T	A	2: 17,379,820	Y65F	possibly damaging	Het
Nelfb	A	T	2: 25,205,159	D339E	possibly damaging	Het
Nlrc3	T	A	16: 3,965,631	I20L	probably benign	Het
Nup205	T	A	6: 35,230,516	M1501K	probably benign	Het
Olfr1487	T	A	19: 13,619,745	N151K	probably damaging	Het
Olfr304	A	T	7: 86,385,768	C297*	probably null	Het
Olfr51	A	G	11: 51,007,500	H176R	probably damaging	Het
Olfr720	A	G	14: 14,175,863	I73T	probably damaging	Het
Olfr725	T	C	14: 50,035,151	D84G	probably benign	Het
Palm3	A	G	8: 84,029,809	E650G	probably damaging	Het
Prps1l1	C	A	12: 34,985,341	L152M	probably damaging	Het
Rgs7bp	C	A	13: 105,053,121	V57F	probably damaging	Het
Scaf8	T	A	17: 3,164,183	V171D	unknown	Het
Sf3a2	T	C	10: 80,803,640	Y155H	probably damaging	Het
Sfrp1	T	G	8: 23,411,984	L67R	probably damaging	Het
Shank2	A	T	7: 144,409,875	I407L	probably benign	Het
Skint6	T	C	4: 112,865,675	T902A	possibly damaging	Het
Skint6	A	C	4: 113,156,494	S353R	possibly damaging	Het
Srrm2	T	A	17: 23,808,083	I87N	unknown	Het
Stard9	G	A	2: 120,704,430	G3723S	probably benign	Het
Svs3b	A	G	2: 164,256,006	S132P	probably damaging	Het
Tbc1d17	G	T	7: 44,843,002	F412L	probably benign	Het
Tex29	A	T	8: 11,854,263	E116D	unknown	Het
Tmem204	T	C	17: 25,080,338	D69G	possibly damaging	Het
Ttll10	G	A	4: 156,044,762	R308C	probably benign	Het
Ttn	T	A	2: 76,747,164	I24462F	probably damaging	Het
Tubg2	A	G	11: 101,161,478	E411G	probably damaging	Het
Ugt2b38	T	C	5: 87,423,771	N134S	probably damaging	Het
Usp31	T	C	7: 121,677,262	T351A	probably damaging	Het
Usp33	T	C	3: 152,360,359	L92S	probably damaging	Het
Vmn2r20	A	T	6: 123,396,470	I471N	probably damaging	Het
Wdfy4	G	A	14: 33,104,115	P1193L		Het
Wnk2	A	G	13: 49,090,983	V459A	probably damaging	Het

Other mutations in Olfr998

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01763:Olfr998	APN	2	85591347	missense	probably benign	0.00
R0091:Olfr998	UTSW	2	85591352	missense	probably benign	0.38
R0241:Olfr998	UTSW	2	85590810	missense	probably benign	0.20
R0241:Olfr998	UTSW	2	85590810	missense	probably benign	0.20
R0268:Olfr998	UTSW	2	85591301	missense	possibly damaging	0.78
R0481:Olfr998	UTSW	2	85591104	missense	possibly damaging	0.94
R1816:Olfr998	UTSW	2	85590925	missense	probably benign	0.00
R1988:Olfr998	UTSW	2	85590641	missense	probably benign	0.00
R2008:Olfr998	UTSW	2	85591422	missense	probably damaging	1.00
R2060:Olfr998	UTSW	2	85591283	missense	possibly damaging	0.78
R2273:Olfr998	UTSW	2	85590588	missense	probably damaging	1.00
R4409:Olfr998	UTSW	2	85590930	missense	probably damaging	1.00
R4783:Olfr998	UTSW	2	85590938	missense	probably benign	0.03
R4785:Olfr998	UTSW	2	85590938	missense	probably benign	0.03
R5098:Olfr998	UTSW	2	85590632	missense	probably benign	0.00
R5176:Olfr998	UTSW	2	85591435	missense	possibly damaging	0.88
R5462:Olfr998	UTSW	2	85591296	missense	probably damaging	1.00
R6092:Olfr998	UTSW	2	85590606	missense	probably benign	0.14
R6278:Olfr998	UTSW	2	85590998	missense	probably benign	0.00
R7022:Olfr998	UTSW	2	85590598	missense	probably benign	0.01
R7673:Olfr998	UTSW	2	85591062	missense	possibly damaging	0.75
R8054:Olfr998	UTSW	2	85590840	missense	probably damaging	1.00
R8940:Olfr998	UTSW	2	85591184	missense	probably benign
R9617:Olfr998	UTSW	2	85590935	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CATAGCTCCTAAGATGCTCTGTG -3'
(R):5'- GAACACTCCTATGGCTCCAG -3'

Sequencing Primer
(F):5'- AGCTCCTAAGATGCTCTGTGATATC -3'
(R):5'- TCCTATGGCTCCAGAAAGAAC -3'

Posted On

2020-06-30