Mutagenetix > Incidental Mutation

Incidental Mutation 'R8118:Wnk2'

631359

Institutional Source

Beutler Lab

Gene Symbol

Wnk2

Ensembl Gene

ENSMUSG00000037989

Gene Name

WNK lysine deficient protein kinase 2

Synonyms

ESTM15, X83337, 1810073P09Rik

MMRRC Submission

067547-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.289) question?

Stock #

R8118 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

49036303-49148014 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 49090983 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 459 (V459A)

Ref Sequence

ENSEMBL: ENSMUSP00000047231 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035538] [ENSMUST00000049265] [ENSMUST00000091623] [ENSMUST00000110096] [ENSMUST00000110097] [ENSMUST00000159559] [ENSMUST00000162403] [ENSMUST00000162581]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000035538
AA Change: V459A

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000047231
Gene: ENSMUSG00000037989
AA Change: V459A

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.5e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.6e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	6.72e-6	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
low complexity region	2033	2053	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000049265
AA Change: V459A

PolyPhen 2 Score 0.985 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049327
Gene: ENSMUSG00000037989
AA Change: V459A

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
S_TKc	195	453	3.3e-19	SMART
Pfam:OSR1_C	474	511	5.6e-19	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	9.51e-6	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
internal_repeat_2	1611	1703	2.12e-5	PROSPERO
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
internal_repeat_2	1923	2023	2.12e-5	PROSPERO
low complexity region	2069	2089	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000091623
AA Change: V459A

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000089212
Gene: ENSMUSG00000037989
AA Change: V459A

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	1.6e-41	PFAM
Pfam:Pkinase	195	453	1e-54	PFAM
Pfam:OSR1_C	474	511	4e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	1.52e-5	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
internal_repeat_2	1611	1713	7.41e-5	PROSPERO
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
internal_repeat_2	1923	2027	7.41e-5	PROSPERO
low complexity region	2117	2137	N/A	INTRINSIC
low complexity region	2191	2202	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000110096
AA Change: V459A

SMART Domains

Protein: ENSMUSP00000105723
Gene: ENSMUSG00000037989
AA Change: V459A

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.6e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.6e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	1015	1038	N/A	INTRINSIC
internal_repeat_1	1064	1087	2.89e-5	PROSPERO
low complexity region	1177	1194	N/A	INTRINSIC
low complexity region	1219	1255	N/A	INTRINSIC
low complexity region	1331	1343	N/A	INTRINSIC
low complexity region	1351	1359	N/A	INTRINSIC
low complexity region	1366	1391	N/A	INTRINSIC
low complexity region	1489	1498	N/A	INTRINSIC
low complexity region	1721	1732	N/A	INTRINSIC
coiled coil region	1737	1768	N/A	INTRINSIC
low complexity region	2018	2038	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000110097
AA Change: V459A

PolyPhen 2 Score 0.828 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000105724
Gene: ENSMUSG00000037989
AA Change: V459A

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.7e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.8e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	1.16e-5	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
internal_repeat_2	1611	1713	5.74e-5	PROSPERO
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
internal_repeat_2	1923	2027	5.74e-5	PROSPERO
low complexity region	2117	2137	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000159559
AA Change: V459A

PolyPhen 2 Score 0.845 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000123915
Gene: ENSMUSG00000037989
AA Change: V459A

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.7e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.8e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	736	N/A	INTRINSIC
low complexity region	759	791	N/A	INTRINSIC
low complexity region	809	819	N/A	INTRINSIC
low complexity region	828	842	N/A	INTRINSIC
low complexity region	863	878	N/A	INTRINSIC
low complexity region	926	942	N/A	INTRINSIC
low complexity region	971	1002	N/A	INTRINSIC
low complexity region	1009	1036	N/A	INTRINSIC
low complexity region	1101	1124	N/A	INTRINSIC
internal_repeat_1	1150	1173	7.19e-6	PROSPERO
low complexity region	1263	1280	N/A	INTRINSIC
low complexity region	1305	1341	N/A	INTRINSIC
low complexity region	1417	1429	N/A	INTRINSIC
low complexity region	1437	1445	N/A	INTRINSIC
low complexity region	1452	1477	N/A	INTRINSIC
low complexity region	1575	1585	N/A	INTRINSIC
internal_repeat_2	1599	1701	3.66e-5	PROSPERO
low complexity region	1808	1819	N/A	INTRINSIC
coiled coil region	1824	1855	N/A	INTRINSIC
internal_repeat_2	1911	2015	3.66e-5	PROSPERO
low complexity region	2105	2125	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000162403
AA Change: V459A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000125539
Gene: ENSMUSG00000037989
AA Change: V459A

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.3e-42	PFAM
Pfam:Pkinase	195	453	1.6e-56	PFAM
Pfam:OSR1_C	474	511	5.3e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	736	N/A	INTRINSIC
low complexity region	759	791	N/A	INTRINSIC
low complexity region	809	819	N/A	INTRINSIC
low complexity region	828	842	N/A	INTRINSIC
low complexity region	863	878	N/A	INTRINSIC
low complexity region	926	942	N/A	INTRINSIC
low complexity region	1003	1026	N/A	INTRINSIC
internal_repeat_1	1052	1075	1.05e-5	PROSPERO
low complexity region	1165	1182	N/A	INTRINSIC
low complexity region	1207	1243	N/A	INTRINSIC
low complexity region	1319	1331	N/A	INTRINSIC
low complexity region	1339	1347	N/A	INTRINSIC
low complexity region	1354	1379	N/A	INTRINSIC
low complexity region	1477	1487	N/A	INTRINSIC
internal_repeat_2	1501	1593	2.32e-5	PROSPERO
low complexity region	1710	1721	N/A	INTRINSIC
coiled coil region	1726	1757	N/A	INTRINSIC
internal_repeat_2	1813	1913	2.32e-5	PROSPERO
low complexity region	1959	1979	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000162581
AA Change: V459A

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000125448
Gene: ENSMUSG00000037989
AA Change: V459A

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.5e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.6e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	6.72e-6	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
low complexity region	2033	2053	N/A	INTRINSIC

Coding Region Coverage

1x: 99.8%
3x: 99.5%
10x: 98.2%
20x: 91.1%

Validation Efficiency

97% (68/70)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a cytoplasmic serine-threonine kinase that belongs to the protein kinase superfamily. The protein plays an important role in the regulation of electrolyte homeostasis, cell signaling survival, and proliferation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts1	T	C	16: 85,795,933 (GRCm38)	D792G	probably damaging	Het
Adcy7	A	G	8: 88,315,756 (GRCm38)	H417R	probably damaging	Het
Agr2	A	G	12: 35,996,107 (GRCm38)	D79G	probably benign	Het
Ankle1	T	C	8: 71,407,635 (GRCm38)	S286P	probably benign	Het
Arhgef11	T	C	3: 87,735,857 (GRCm38)	S1488P	probably damaging	Het
Atp6v0a2	T	C	5: 124,712,773 (GRCm38)	M421T	probably damaging	Het
Cdk17	C	A	10: 93,216,390 (GRCm38)	Q111K	possibly damaging	Het
Cobl	A	G	11: 12,254,834 (GRCm38)	S623P	probably benign	Het
Dgka	C	T	10: 128,722,449 (GRCm38)		probably null	Het
Dsc2	C	T	18: 20,032,274 (GRCm38)	G881R	possibly damaging	Het
Dsg2	A	G	18: 20,582,801 (GRCm38)	I267V	probably benign	Het
Exoc4	T	C	6: 33,971,918 (GRCm38)	Y899H	probably damaging	Het
Fat3	A	G	9: 15,960,104 (GRCm38)	F3664L	probably benign	Het
Fbxo47	C	T	11: 97,879,515 (GRCm38)	C17Y	probably benign	Het
Gm2888	G	T	14: 3,037,628 (GRCm38)	V207F	probably benign	Het
Gm7030	C	T	17: 36,127,690 (GRCm38)	V270M	probably damaging	Het
Gpr61	A	G	3: 108,150,572 (GRCm38)	S258P	probably damaging	Het
Hectd4	C	T	5: 121,286,376 (GRCm38)	H700Y	probably benign	Het
Hs3st2	C	T	7: 121,397,428 (GRCm38)	T154I	probably benign	Het
Inf2	A	T	12: 112,601,437 (GRCm38)	H167L	probably damaging	Het
Ints3	C	T	3: 90,400,299 (GRCm38)		probably null	Het
Ippk	C	T	13: 49,446,342 (GRCm38)	P226S		Het
Itgal	C	A	7: 127,311,245 (GRCm38)	Q509K	probably benign	Het
Klhl20	A	T	1: 161,098,401 (GRCm38)		probably null	Het
Krtap4-13	C	T	11: 99,809,398 (GRCm38)	C145Y	unknown	Het
Large1	A	G	8: 73,131,944 (GRCm38)	S99P	probably benign	Het
Lexm	T	A	4: 106,613,398 (GRCm38)	R192S	possibly damaging	Het
Lrba	A	T	3: 86,354,226 (GRCm38)	I1496L	probably benign	Het
Map2	A	T	1: 66,425,391 (GRCm38)	I1647F	probably damaging	Het
Map3k10	A	G	7: 27,673,417 (GRCm38)	V203A	possibly damaging	Het
Mcmdc2	T	A	1: 9,916,374 (GRCm38)	N166K	possibly damaging	Het
Mlxipl	T	G	5: 135,137,248 (GRCm38)	L828R	possibly damaging	Het
Mnat1	A	G	12: 73,219,090 (GRCm38)	I253V	probably benign	Het
Mtfp1	A	G	11: 4,093,910 (GRCm38)	S107P	probably damaging	Het
Nebl	T	A	2: 17,379,820 (GRCm38)	Y65F	possibly damaging	Het
Nelfb	A	T	2: 25,205,159 (GRCm38)	D339E	possibly damaging	Het
Nlrc3	T	A	16: 3,965,631 (GRCm38)	I20L	probably benign	Het
Nup205	T	A	6: 35,230,516 (GRCm38)	M1501K	probably benign	Het
Olfr1487	T	A	19: 13,619,745 (GRCm38)	N151K	probably damaging	Het
Olfr304	A	T	7: 86,385,768 (GRCm38)	C297*	probably null	Het
Olfr51	A	G	11: 51,007,500 (GRCm38)	H176R	probably damaging	Het
Olfr720	A	G	14: 14,175,863 (GRCm38)	I73T	probably damaging	Het
Olfr725	T	C	14: 50,035,151 (GRCm38)	D84G	probably benign	Het
Olfr998	T	A	2: 85,590,988 (GRCm38)	Y149*	probably null	Het
Palm3	A	G	8: 84,029,809 (GRCm38)	E650G	probably damaging	Het
Prps1l1	C	A	12: 34,985,341 (GRCm38)	L152M	probably damaging	Het
Rgs7bp	C	A	13: 105,053,121 (GRCm38)	V57F	probably damaging	Het
Scaf8	T	A	17: 3,164,183 (GRCm38)	V171D	unknown	Het
Sf3a2	T	C	10: 80,803,640 (GRCm38)	Y155H	probably damaging	Het
Sfrp1	T	G	8: 23,411,984 (GRCm38)	L67R	probably damaging	Het
Shank2	A	T	7: 144,409,875 (GRCm38)	I407L	probably benign	Het
Skint6	A	C	4: 113,156,494 (GRCm38)	S353R	possibly damaging	Het
Skint6	T	C	4: 112,865,675 (GRCm38)	T902A	possibly damaging	Het
Srrm2	T	A	17: 23,808,083 (GRCm38)	I87N	unknown	Het
Stard9	G	A	2: 120,704,430 (GRCm38)	G3723S	probably benign	Het
Svs3b	A	G	2: 164,256,006 (GRCm38)	S132P	probably damaging	Het
Tbc1d17	G	T	7: 44,843,002 (GRCm38)	F412L	probably benign	Het
Tex29	A	T	8: 11,854,263 (GRCm38)	E116D	unknown	Het
Tmem204	T	C	17: 25,080,338 (GRCm38)	D69G	possibly damaging	Het
Ttll10	G	A	4: 156,044,762 (GRCm38)	R308C	probably benign	Het
Ttn	T	A	2: 76,747,164 (GRCm38)	I24462F	probably damaging	Het
Tubg2	A	G	11: 101,161,478 (GRCm38)	E411G	probably damaging	Het
Ugt2b38	T	C	5: 87,423,771 (GRCm38)	N134S	probably damaging	Het
Usp31	T	C	7: 121,677,262 (GRCm38)	T351A	probably damaging	Het
Usp33	T	C	3: 152,360,359 (GRCm38)	L92S	probably damaging	Het
Vmn2r20	A	T	6: 123,396,470 (GRCm38)	I471N	probably damaging	Het
Wdfy4	G	A	14: 33,104,115 (GRCm38)	P1193L		Het

Other mutations in Wnk2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01518:Wnk2	APN	13	49,068,192 (GRCm38)	missense	possibly damaging	0.89
IGL01575:Wnk2	APN	13	49,146,676 (GRCm38)	missense	probably damaging	1.00
IGL01601:Wnk2	APN	13	49,076,562 (GRCm38)	missense	probably damaging	1.00
IGL01775:Wnk2	APN	13	49,071,110 (GRCm38)	missense	probably damaging	1.00
IGL02013:Wnk2	APN	13	49,082,034 (GRCm38)	missense	possibly damaging	0.46
IGL02016:Wnk2	APN	13	49,056,905 (GRCm38)	missense	probably damaging	1.00
IGL02167:Wnk2	APN	13	49,071,125 (GRCm38)	critical splice acceptor site	probably null
IGL02174:Wnk2	APN	13	49,057,167 (GRCm38)	missense	probably damaging	1.00
IGL02210:Wnk2	APN	13	49,090,869 (GRCm38)	missense	probably damaging	0.98
IGL02228:Wnk2	APN	13	49,056,940 (GRCm38)	missense	probably damaging	1.00
IGL02282:Wnk2	APN	13	49,068,125 (GRCm38)	missense	probably damaging	1.00
IGL02319:Wnk2	APN	13	49,061,438 (GRCm38)	missense	possibly damaging	0.73
IGL02394:Wnk2	APN	13	49,081,899 (GRCm38)	splice site	probably null
IGL02624:Wnk2	APN	13	49,102,802 (GRCm38)	missense	probably damaging	1.00
IGL02743:Wnk2	APN	13	49,095,444 (GRCm38)	missense	probably damaging	1.00
IGL03012:Wnk2	APN	13	49,044,389 (GRCm38)	missense	probably damaging	0.99
IGL03166:Wnk2	APN	13	49,071,044 (GRCm38)	nonsense	probably null
R0034:Wnk2	UTSW	13	49,068,080 (GRCm38)	missense	possibly damaging	0.64
R0385:Wnk2	UTSW	13	49,068,128 (GRCm38)	missense	probably damaging	1.00
R0423:Wnk2	UTSW	13	49,095,418 (GRCm38)	missense	possibly damaging	0.91
R0504:Wnk2	UTSW	13	49,085,396 (GRCm38)	missense	probably damaging	1.00
R0504:Wnk2	UTSW	13	49,085,394 (GRCm38)	missense	possibly damaging	0.92
R0653:Wnk2	UTSW	13	49,057,016 (GRCm38)	missense	possibly damaging	0.85
R1135:Wnk2	UTSW	13	49,076,558 (GRCm38)	missense	probably damaging	1.00
R1445:Wnk2	UTSW	13	49,071,110 (GRCm38)	missense	probably damaging	1.00
R1464:Wnk2	UTSW	13	49,081,975 (GRCm38)	missense	probably damaging	1.00
R1464:Wnk2	UTSW	13	49,081,975 (GRCm38)	missense	probably damaging	1.00
R1468:Wnk2	UTSW	13	49,082,095 (GRCm38)	missense	probably damaging	0.99
R1468:Wnk2	UTSW	13	49,082,095 (GRCm38)	missense	probably damaging	0.99
R1480:Wnk2	UTSW	13	49,057,232 (GRCm38)	missense	probably damaging	1.00
R1605:Wnk2	UTSW	13	49,060,894 (GRCm38)	missense	probably damaging	1.00
R1719:Wnk2	UTSW	13	49,060,726 (GRCm38)	missense	possibly damaging	0.76
R1891:Wnk2	UTSW	13	49,052,724 (GRCm38)	nonsense	probably null
R1966:Wnk2	UTSW	13	49,039,011 (GRCm38)	missense	probably damaging	0.96
R2001:Wnk2	UTSW	13	49,078,682 (GRCm38)	missense	possibly damaging	0.61
R2310:Wnk2	UTSW	13	49,050,577 (GRCm38)	missense	probably damaging	0.97
R2356:Wnk2	UTSW	13	49,039,168 (GRCm38)	nonsense	probably null
R2406:Wnk2	UTSW	13	49,061,488 (GRCm38)	missense	possibly damaging	0.86
R2519:Wnk2	UTSW	13	49,071,029 (GRCm38)	missense	probably damaging	0.99
R3962:Wnk2	UTSW	13	49,070,977 (GRCm38)	missense	probably damaging	1.00
R4160:Wnk2	UTSW	13	49,090,837 (GRCm38)	missense	probably damaging	1.00
R4161:Wnk2	UTSW	13	49,090,837 (GRCm38)	missense	probably damaging	1.00
R4226:Wnk2	UTSW	13	49,090,837 (GRCm38)	missense	probably damaging	1.00
R4227:Wnk2	UTSW	13	49,090,837 (GRCm38)	missense	probably damaging	1.00
R4234:Wnk2	UTSW	13	49,061,128 (GRCm38)	missense	probably benign	0.33
R4304:Wnk2	UTSW	13	49,090,837 (GRCm38)	missense	probably damaging	1.00
R4308:Wnk2	UTSW	13	49,090,837 (GRCm38)	missense	probably damaging	1.00
R4584:Wnk2	UTSW	13	49,090,837 (GRCm38)	missense	probably damaging	1.00
R4655:Wnk2	UTSW	13	49,056,883 (GRCm38)	missense	probably damaging	1.00
R4715:Wnk2	UTSW	13	49,147,232 (GRCm38)	start codon destroyed	unknown
R4887:Wnk2	UTSW	13	49,071,002 (GRCm38)	missense	probably damaging	1.00
R4888:Wnk2	UTSW	13	49,071,002 (GRCm38)	missense	probably damaging	1.00
R4945:Wnk2	UTSW	13	49,057,246 (GRCm38)	missense	probably damaging	1.00
R5182:Wnk2	UTSW	13	49,061,161 (GRCm38)	missense	possibly damaging	0.92
R5243:Wnk2	UTSW	13	49,072,578 (GRCm38)	missense	possibly damaging	0.51
R5370:Wnk2	UTSW	13	49,102,961 (GRCm38)	missense	probably damaging	1.00
R5771:Wnk2	UTSW	13	49,102,800 (GRCm38)	missense	probably damaging	1.00
R5877:Wnk2	UTSW	13	49,067,306 (GRCm38)	missense	probably damaging	0.98
R5900:Wnk2	UTSW	13	49,102,832 (GRCm38)	missense	probably damaging	1.00
R5905:Wnk2	UTSW	13	49,076,345 (GRCm38)	missense	probably damaging	0.99
R5912:Wnk2	UTSW	13	49,060,774 (GRCm38)	missense	probably damaging	1.00
R5915:Wnk2	UTSW	13	49,078,085 (GRCm38)	missense	probably damaging	0.99
R6028:Wnk2	UTSW	13	49,076,345 (GRCm38)	missense	probably damaging	0.99
R6074:Wnk2	UTSW	13	49,051,399 (GRCm38)	missense	probably damaging	1.00
R6171:Wnk2	UTSW	13	49,060,832 (GRCm38)	missense	probably damaging	1.00
R6368:Wnk2	UTSW	13	49,061,338 (GRCm38)	missense	probably damaging	0.99
R6467:Wnk2	UTSW	13	49,146,605 (GRCm38)	missense	probably damaging	1.00
R6501:Wnk2	UTSW	13	49,146,683 (GRCm38)	missense	probably damaging	1.00
R6849:Wnk2	UTSW	13	49,067,358 (GRCm38)	missense	probably damaging	1.00
R6898:Wnk2	UTSW	13	49,071,081 (GRCm38)	missense	probably damaging	1.00
R6949:Wnk2	UTSW	13	49,101,140 (GRCm38)	missense	probably damaging	1.00
R7011:Wnk2	UTSW	13	49,071,091 (GRCm38)	missense	probably damaging	0.99
R7097:Wnk2	UTSW	13	49,102,838 (GRCm38)	missense	possibly damaging	0.86
R7121:Wnk2	UTSW	13	49,147,177 (GRCm38)	missense	probably benign	0.26
R7123:Wnk2	UTSW	13	49,081,986 (GRCm38)	missense	possibly damaging	0.90
R7423:Wnk2	UTSW	13	49,038,132 (GRCm38)	missense	probably benign	0.07
R7502:Wnk2	UTSW	13	49,147,244 (GRCm38)	splice site	probably null
R7529:Wnk2	UTSW	13	49,100,981 (GRCm38)	missense	possibly damaging	0.50
R7751:Wnk2	UTSW	13	49,078,017 (GRCm38)	missense	unknown
R7979:Wnk2	UTSW	13	49,095,408 (GRCm38)	missense	probably damaging	1.00
R8121:Wnk2	UTSW	13	49,060,939 (GRCm38)	nonsense	probably null
R8155:Wnk2	UTSW	13	49,039,101 (GRCm38)	missense	unknown
R8329:Wnk2	UTSW	13	49,095,438 (GRCm38)	missense	probably damaging	1.00
R8334:Wnk2	UTSW	13	49,050,482 (GRCm38)	critical splice donor site	probably null
R8872:Wnk2	UTSW	13	49,057,484 (GRCm38)	missense	probably benign	0.00
R8919:Wnk2	UTSW	13	49,068,235 (GRCm38)	missense	possibly damaging	0.86
R9091:Wnk2	UTSW	13	49,071,029 (GRCm38)	missense	probably benign	0.41
R9234:Wnk2	UTSW	13	49,070,798 (GRCm38)	missense	probably damaging	0.99
R9262:Wnk2	UTSW	13	49,067,954 (GRCm38)	missense	probably benign	0.12
R9268:Wnk2	UTSW	13	49,082,031 (GRCm38)	missense	possibly damaging	0.82
R9270:Wnk2	UTSW	13	49,071,029 (GRCm38)	missense	probably benign	0.41
R9386:Wnk2	UTSW	13	49,067,346 (GRCm38)	missense	probably damaging	0.98
R9582:Wnk2	UTSW	13	49,057,499 (GRCm38)	missense	probably benign	0.01
R9617:Wnk2	UTSW	13	49,038,977 (GRCm38)	missense	unknown
R9625:Wnk2	UTSW	13	49,100,969 (GRCm38)	missense	probably benign	0.20
R9794:Wnk2	UTSW	13	49,076,198 (GRCm38)	missense	probably benign	0.02
RF023:Wnk2	UTSW	13	49,146,779 (GRCm38)	missense	probably benign	0.00
X0025:Wnk2	UTSW	13	49,060,942 (GRCm38)	missense	probably damaging	0.99
X0063:Wnk2	UTSW	13	49,038,977 (GRCm38)	missense	unknown
Z1176:Wnk2	UTSW	13	49,038,061 (GRCm38)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- GTGTGCACATTACCATTTCCTG -3'
(R):5'- GGAATGCTAGTGTGCACATCTG -3'

Sequencing Primer
(F):5'- ATTTCCTGGGCCACCTCG -3'
(R):5'- ATCTGTGTCCTACCCACGGG -3'

Posted On

2020-06-30