Mutagenetix > Incidental Mutation

Incidental Mutation 'R8122:Taar8c'

631557

Institutional Source

Beutler Lab

Gene Symbol

Taar8c

Ensembl Gene

ENSMUSG00000100004

Gene Name

trace amine-associated receptor 8C

Synonyms

MMRRC Submission

067551-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.077) question?

Stock #

R8122 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

23976776-23977810 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 23977107 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Asparagine at position 235 (S235N)

Ref Sequence

ENSEMBL: ENSMUSP00000133193 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170267]

AlphaFold

Q5QD05

Predicted Effect

probably benign
Transcript: ENSMUST00000170267
AA Change: S235N

PolyPhen 2 Score 0.144 (Sensitivity: 0.92; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000133193
Gene: ENSMUSG00000100004
AA Change: S235N

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	40	244	2.7e-8	PFAM
Pfam:7TM_GPCR_Srsx	42	327	4.9e-15	PFAM
Pfam:7tm_1	48	312	2.3e-57	PFAM

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 99.0%
20x: 97.5%

Validation Efficiency

98% (47/48)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abat	A	G	16: 8,433,761 (GRCm39)	Y426C	probably damaging	Het
Acsbg3	T	A	17: 57,193,670 (GRCm39)	V672E	possibly damaging	Het
Adgrv1	A	G	13: 81,419,037 (GRCm39)	V5986A	probably damaging	Het
Adgrv1	A	T	13: 81,588,344 (GRCm39)	V4414E	possibly damaging	Het
Ahnak2	T	C	12: 112,742,510 (GRCm39)	S521G	possibly damaging	Het
Amer2	A	T	14: 60,616,791 (GRCm39)	M329L	possibly damaging	Het
Arhgap20	A	G	9: 51,761,293 (GRCm39)	N1048S	probably damaging	Het
Asah1	T	C	8: 41,796,767 (GRCm39)	E305G	probably benign	Het
AW551984	T	A	9: 39,510,665 (GRCm39)	K223N	probably damaging	Het
Bptf	C	A	11: 106,927,417 (GRCm39)		probably null	Het
C1qtnf6	T	C	15: 78,411,446 (GRCm39)	N77D	probably benign	Het
Capn13	T	A	17: 73,674,205 (GRCm39)	I83F	probably damaging	Het
Cd6	A	G	19: 10,770,231 (GRCm39)	F487L	probably damaging	Het
Cit	T	C	5: 116,107,069 (GRCm39)	V1067A	probably damaging	Het
Cspg4b	A	T	13: 113,455,442 (GRCm39)	D496V		Het
Csrnp1	T	C	9: 119,802,273 (GRCm39)	D262G	probably damaging	Het
Cyp2d11	T	A	15: 82,276,744 (GRCm39)	Q65L	probably benign	Het
Dbt	T	A	3: 116,313,891 (GRCm39)	C19*	probably null	Het
Dgkg	A	C	16: 22,385,295 (GRCm39)		probably null	Het
Eif4g2	T	C	7: 110,677,760 (GRCm39)	I118V	possibly damaging	Het
Evl	C	T	12: 108,647,783 (GRCm39)	R295*	probably null	Het
Gpr158	A	T	2: 21,831,674 (GRCm39)	M925L	probably benign	Het
Kcnn2	T	G	18: 45,810,005 (GRCm39)	V414G	probably damaging	Het
Lama2	G	A	10: 26,930,592 (GRCm39)	H2055Y	possibly damaging	Het
Loxl3	T	A	6: 83,026,240 (GRCm39)	W443R	probably damaging	Het
Mab21l1	A	T	3: 55,690,905 (GRCm39)	D164V	probably benign	Het
Mroh7	T	C	4: 106,559,726 (GRCm39)	T734A	probably damaging	Het
Or2y1f	C	A	11: 49,184,401 (GRCm39)	N84K	probably damaging	Het
Or8g35	A	G	9: 39,381,822 (GRCm39)	S67P	probably damaging	Het
Pkhd1	C	T	1: 20,632,682 (GRCm39)	E578K	probably damaging	Het
Pld2	T	A	11: 70,432,259 (GRCm39)	L126*	probably null	Het
Polk	A	T	13: 96,620,291 (GRCm39)	N716K	probably benign	Het
Ppl	C	T	16: 4,906,725 (GRCm39)	R1190H	probably damaging	Het
Prom1	T	C	5: 44,170,295 (GRCm39)	K669E	probably benign	Het
Sag	A	G	1: 87,762,289 (GRCm39)	D318G	probably damaging	Het
Scfd1	T	A	12: 51,480,052 (GRCm39)	V528E	possibly damaging	Het
Scn4a	A	G	11: 106,221,157 (GRCm39)	L834P	probably benign	Het
Shank1	G	T	7: 43,983,015 (GRCm39)	G637V	unknown	Het
Slc12a6	A	G	2: 112,097,167 (GRCm39)	M1V	probably null	Het
St6gal1	A	G	16: 23,173,644 (GRCm39)	K242R	probably benign	Het
Syt3	A	G	7: 44,045,153 (GRCm39)	Y495C	probably damaging	Het
Tmem183a	A	T	1: 134,282,503 (GRCm39)	C201S	probably benign	Het
Top2a	T	C	11: 98,889,993 (GRCm39)	I1237V	probably benign	Het
Trub1	G	T	19: 57,473,563 (GRCm39)	V207L	probably benign	Het
Ttll13	T	A	7: 79,909,217 (GRCm39)	I634N	probably benign	Het
Ube2j1	T	G	4: 33,045,145 (GRCm39)	N208K	probably benign	Het
Wdr17	A	G	8: 55,118,011 (GRCm39)	S569P	probably damaging	Het

Other mutations in Taar8c

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00816:Taar8c	APN	10	23,977,173 (GRCm39)	missense	probably damaging	0.99
IGL01733:Taar8c	APN	10	23,977,155 (GRCm39)	missense	possibly damaging	0.86
R0669:Taar8c	UTSW	10	23,977,401 (GRCm39)	missense	probably damaging	1.00
R1186:Taar8c	UTSW	10	23,977,463 (GRCm39)	nonsense	probably null
R1452:Taar8c	UTSW	10	23,977,508 (GRCm39)	missense	probably benign	0.00
R2256:Taar8c	UTSW	10	23,976,969 (GRCm39)	missense	probably benign	0.01
R4364:Taar8c	UTSW	10	23,977,477 (GRCm39)	missense	probably benign	0.02
R4904:Taar8c	UTSW	10	23,977,147 (GRCm39)	missense	probably benign	0.01
R5314:Taar8c	UTSW	10	23,977,246 (GRCm39)	missense	probably damaging	1.00
R6163:Taar8c	UTSW	10	23,977,116 (GRCm39)	missense	probably benign	0.00
R6631:Taar8c	UTSW	10	23,977,701 (GRCm39)	missense	probably benign	0.01
R7428:Taar8c	UTSW	10	23,977,446 (GRCm39)	missense	probably damaging	1.00
R7497:Taar8c	UTSW	10	23,977,116 (GRCm39)	missense	probably benign	0.00
R7525:Taar8c	UTSW	10	23,977,764 (GRCm39)	missense	probably benign	0.03
R8280:Taar8c	UTSW	10	23,976,835 (GRCm39)	missense	probably benign	0.37
R8293:Taar8c	UTSW	10	23,977,015 (GRCm39)	missense	probably benign	0.00
R8772:Taar8c	UTSW	10	23,977,705 (GRCm39)	missense	probably benign
R9165:Taar8c	UTSW	10	23,977,500 (GRCm39)	missense	probably damaging	1.00
Z1177:Taar8c	UTSW	10	23,977,305 (GRCm39)	missense	probably benign	0.37
Z1177:Taar8c	UTSW	10	23,977,148 (GRCm39)	missense	probably benign	0.15

Predicted Primers

PCR Primer
(F):5'- TTTCATAGACATAGGCAGGAGTG -3'
(R):5'- ACAGCAGTGCAGTGTTCTAC -3'

Sequencing Primer
(F):5'- TGATGAAGCCCATGAAAGCATC -3'
(R):5'- TGTTCTACACAGGCATCAGTG -3'

Posted On

2020-06-30