Incidental Mutation 'R8124:Klf15'
ID631640
Institutional Source Beutler Lab
Gene Symbol Klf15
Ensembl Gene ENSMUSG00000030087
Gene NameKruppel-like factor 15
SynonymsKKLF, 1810013I09Rik, CKLF
MMRRC Submission
Accession Numbers
Is this an essential gene? Possibly essential (E-score: 0.621) question?
Stock #R8124 (G1)
Quality Score225.009
Status Validated
Chromosome6
Chromosomal Location90462576-90475238 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 90466881 bp
ZygosityHeterozygous
Amino Acid Change Phenylalanine to Serine at position 146 (F146S)
Ref Sequence ENSEMBL: ENSMUSP00000032174 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000032174] [ENSMUST00000113530] [ENSMUST00000203039] [ENSMUST00000203607]
Predicted Effect probably damaging
Transcript: ENSMUST00000032174
AA Change: F146S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000032174
Gene: ENSMUSG00000030087
AA Change: F146S

DomainStartEndE-ValueType
low complexity region 87 100 N/A INTRINSIC
low complexity region 141 154 N/A INTRINSIC
low complexity region 192 211 N/A INTRINSIC
low complexity region 291 305 N/A INTRINSIC
ZnF_C2H2 320 344 1.26e-2 SMART
ZnF_C2H2 350 374 1.98e-4 SMART
ZnF_C2H2 380 402 1.13e-4 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000113530
AA Change: F146S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000109158
Gene: ENSMUSG00000030087
AA Change: F146S

DomainStartEndE-ValueType
low complexity region 87 100 N/A INTRINSIC
low complexity region 141 154 N/A INTRINSIC
low complexity region 192 211 N/A INTRINSIC
low complexity region 291 305 N/A INTRINSIC
ZnF_C2H2 320 344 1.26e-2 SMART
ZnF_C2H2 350 374 1.98e-4 SMART
ZnF_C2H2 380 402 1.13e-4 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000203039
AA Change: F146S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000144962
Gene: ENSMUSG00000030087
AA Change: F146S

DomainStartEndE-ValueType
low complexity region 87 100 N/A INTRINSIC
low complexity region 141 154 N/A INTRINSIC
low complexity region 192 211 N/A INTRINSIC
low complexity region 291 305 N/A INTRINSIC
ZnF_C2H2 320 344 1.26e-2 SMART
ZnF_C2H2 350 374 1.98e-4 SMART
ZnF_C2H2 380 402 1.13e-4 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000203607
AA Change: F146S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000144808
Gene: ENSMUSG00000030087
AA Change: F146S

DomainStartEndE-ValueType
low complexity region 87 100 N/A INTRINSIC
low complexity region 141 154 N/A INTRINSIC
low complexity region 192 211 N/A INTRINSIC
low complexity region 291 305 N/A INTRINSIC
ZnF_C2H2 320 344 1.26e-2 SMART
ZnF_C2H2 350 374 1.98e-4 SMART
ZnF_C2H2 380 402 1.13e-4 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000205136
Coding Region Coverage
  • 1x: 99.8%
  • 3x: 99.6%
  • 10x: 98.6%
  • 20x: 94.5%
Validation Efficiency 98% (51/52)
MGI Phenotype PHENOTYPE: Mice homozygous for a null allele display impaired gluconeogenesis with severe fasting induce hypoglycemia. Homozygotes are also more sensitive to induced cardiac stress and display mild cardiac and aortic abnormalities. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 51 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Agap3 T A 5: 24,478,130 I320N probably benign Het
Ahnak G A 19: 9,007,123 G1924S probably damaging Het
Ap2a2 G A 7: 141,598,844 R141H probably benign Het
Atf7ip2 T C 16: 10,209,135 V89A possibly damaging Het
Clpsl2 G A 17: 28,550,728 G55R probably damaging Het
Csnk2a2 G A 8: 95,455,947 P296L Het
Dab2 T A 15: 6,429,397 C263* probably null Het
Dao AGG AG 5: 114,015,209 probably benign Het
Ddx60 C T 8: 61,983,911 A965V probably benign Het
Dennd5a A T 7: 109,897,935 I1119N probably damaging Het
Dpy30 A G 17: 74,316,104 probably benign Het
Epg5 G A 18: 77,964,996 A780T probably benign Het
Etl4 A G 2: 20,806,640 D1546G probably benign Het
Evl C T 12: 108,681,524 R295* probably null Het
F13a1 T C 13: 37,025,805 K62R probably damaging Het
Gm2832 T C 14: 41,278,937 M44T Het
Gm884 A G 11: 103,620,431 V237A unknown Het
Gm9195 T C 14: 72,442,623 I2249V probably benign Het
Hmcn2 T C 2: 31,400,124 V2323A probably benign Het
Ice2 T A 9: 69,400,495 N20K probably damaging Het
Lgi3 A G 14: 70,534,738 Y241C probably damaging Het
Lig4 A T 8: 9,972,954 D275E probably damaging Het
Lrit1 T A 14: 37,062,048 S444R probably benign Het
Mip T C 10: 128,226,201 V107A possibly damaging Het
Myo15 G A 11: 60,507,453 V1581M Het
Nckap1l A G 15: 103,473,821 D481G possibly damaging Het
Obox3 A T 7: 15,589,949 probably null Het
Olfr1438-ps1 T A 19: 12,333,470 N129Y probably damaging Het
Olfr491 C A 7: 108,317,777 N294K possibly damaging Het
Olfr707 G A 7: 106,891,881 T76I possibly damaging Het
Olfr749 C T 14: 50,736,286 R292H probably benign Het
Olfr944 T C 9: 39,217,671 F105L probably benign Het
Papd7 G A 13: 69,533,597 probably benign Het
Pilrb2 T A 5: 137,871,044 E98V probably damaging Het
Plekhm1 A G 11: 103,366,949 V1053A probably benign Het
Prdm2 A T 4: 143,135,265 V485E probably damaging Het
Riox2 G A 16: 59,486,591 E282K probably benign Het
Rps6ka2 T A 17: 7,281,829 V379E possibly damaging Het
Skiv2l2 A C 13: 112,927,337 D7E probably benign Het
Slc4a7 G A 14: 14,729,211 E20K possibly damaging Het
Sned1 G A 1: 93,282,989 probably null Het
Spata3 C T 1: 86,024,353 R110C unknown Het
St6gal1 G A 16: 23,357,835 A393T probably benign Het
Tmem132d T C 5: 127,792,560 Q570R probably damaging Het
Tmem87a T C 2: 120,392,195 T168A probably benign Het
Trank1 A G 9: 111,378,927 R1747G probably benign Het
Txk C T 5: 72,703,263 probably null Het
Vcan T A 13: 89,704,254 L862F possibly damaging Het
Wdr64 A T 1: 175,799,278 probably null Het
Zfp106 G T 2: 120,524,331 Q1343K probably benign Het
Zfp719 A G 7: 43,589,890 T301A probably benign Het
Other mutations in Klf15
AlleleSourceChrCoordTypePredicted EffectPPH Score
R3425:Klf15 UTSW 6 90466820 missense probably benign 0.01
R5443:Klf15 UTSW 6 90467360 missense possibly damaging 0.50
R5985:Klf15 UTSW 6 90466721 missense possibly damaging 0.69
R6171:Klf15 UTSW 6 90466619 missense possibly damaging 0.59
R6529:Klf15 UTSW 6 90467412 missense probably damaging 1.00
R7861:Klf15 UTSW 6 90466838 missense probably benign 0.00
R8082:Klf15 UTSW 6 90466484 missense possibly damaging 0.95
R8397:Klf15 UTSW 6 90466796 missense probably damaging 0.99
R8486:Klf15 UTSW 6 90467178 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GGCAGCATCTTGGATTTCCTCC -3'
(R):5'- ACAGGCTGGATCTGCAGTAG -3'

Sequencing Primer
(F):5'- GGATTTCCTCCTGTCCCGGG -3'
(R):5'- ACCTGCACTTTGGGCAC -3'
Posted On2020-06-30