Mutagenetix > Incidental Mutation

Incidental Mutation 'R8130:Zfp934'

631991

Institutional Source

Beutler Lab

Gene Symbol

Zfp934

Ensembl Gene

ENSMUSG00000074865

Gene Name

zinc finger protein 934

Synonyms

6720457D02Rik

MMRRC Submission

067559-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.126) question?

Stock #

R8130 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

62664609-62727170 bp(-) (GRCm39)

Type of Mutation

critical splice donor site (1 bp from exon)

DNA Base Change (assembly)

C to A at 62667985 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000152181 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076195] [ENSMUST00000082203] [ENSMUST00000101547] [ENSMUST00000220648] [ENSMUST00000221250] [ENSMUST00000222429] [ENSMUST00000222746] [ENSMUST00000222852]

AlphaFold

K7N6H3

Predicted Effect

probably benign
Transcript: ENSMUST00000076195

SMART Domains

Protein: ENSMUSP00000075552
Gene: ENSMUSG00000055228

Domain	Start	End	E-Value	Type
KRAB	4	66	8.26e-16	SMART
ZnF_C2H2	133	155	2.09e-3	SMART
ZnF_C2H2	161	183	1.4e-4	SMART
ZnF_C2H2	189	211	2.57e-3	SMART
ZnF_C2H2	217	239	5.5e-3	SMART
ZnF_C2H2	245	267	7.26e-3	SMART
ZnF_C2H2	273	295	1.4e-4	SMART
ZnF_C2H2	301	323	8.6e-5	SMART
ZnF_C2H2	329	351	1.2e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000082203

SMART Domains

Protein: ENSMUSP00000080837
Gene: ENSMUSG00000074865

Domain	Start	End	E-Value	Type
Blast:KRAB	1	34	3e-16	BLAST
ZnF_C2H2	101	123	1.04e-3	SMART
ZnF_C2H2	129	149	1.68e1	SMART
ZnF_C2H2	157	179	2.75e-3	SMART
ZnF_C2H2	185	207	1.98e-4	SMART
ZnF_C2H2	213	235	3.21e-4	SMART
ZnF_C2H2	241	263	3.44e-4	SMART
ZnF_C2H2	269	292	4.98e-1	SMART
ZnF_C2H2	298	320	4.87e-4	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000101547

SMART Domains

Protein: ENSMUSP00000099083
Gene: ENSMUSG00000074865

Domain	Start	End	E-Value	Type
KRAB	3	65	5.01e-15	SMART
ZnF_C2H2	132	154	1.04e-3	SMART
ZnF_C2H2	160	180	1.68e1	SMART
ZnF_C2H2	188	210	2.75e-3	SMART
ZnF_C2H2	216	238	1.98e-4	SMART
ZnF_C2H2	244	266	3.21e-4	SMART
ZnF_C2H2	272	294	3.44e-4	SMART
ZnF_C2H2	300	322	2.27e-4	SMART
ZnF_C2H2	328	350	3.44e-4	SMART
ZnF_C2H2	356	378	9.22e-5	SMART
ZnF_C2H2	384	406	2.43e-4	SMART
ZnF_C2H2	412	434	1.3e-4	SMART
ZnF_C2H2	440	462	4.87e-4	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000220648

Predicted Effect

probably benign
Transcript: ENSMUST00000221250

Predicted Effect

probably benign
Transcript: ENSMUST00000222429

Predicted Effect

probably benign
Transcript: ENSMUST00000222746

Predicted Effect

probably null
Transcript: ENSMUST00000222852

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 99.1%
20x: 97.3%

Validation Efficiency

96% (52/54)

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700123K08Rik	T	C	5: 138,561,271 (GRCm39)	I130M	probably damaging	Het
4921539E11Rik	T	C	4: 103,092,895 (GRCm39)	D142G	probably damaging	Het
Abcb10	C	T	8: 124,691,757 (GRCm39)	A403T		Het
Arfgef2	T	A	2: 166,678,170 (GRCm39)	S218T	possibly damaging	Het
Armc8	C	T	9: 99,433,600 (GRCm39)	V40I	probably benign	Het
Caps2	A	T	10: 112,018,381 (GRCm39)	D177V	probably benign	Het
Cdh8	A	T	8: 99,757,676 (GRCm39)	F641I	probably damaging	Het
Cemip	T	C	7: 83,596,384 (GRCm39)	S1127G	probably benign	Het
Cmtm1	C	A	8: 105,036,088 (GRCm39)	Q180H	unknown	Het
Col15a1	C	T	4: 47,312,196 (GRCm39)	T1337I	probably damaging	Het
Col18a1	A	T	10: 76,910,284 (GRCm39)	M555K	probably benign	Het
Ctrb1	A	G	8: 112,415,823 (GRCm39)	F89L	possibly damaging	Het
Disp1	T	C	1: 182,917,199 (GRCm39)	T76A	probably benign	Het
Dpep1	T	C	8: 123,926,965 (GRCm39)	V263A	probably damaging	Het
Dysf	G	A	6: 84,114,358 (GRCm39)	E1216K	probably damaging	Het
F830016B08Rik	A	G	18: 60,433,052 (GRCm39)	Y45C	probably benign	Het
Fam186a	CGG	CG	15: 99,841,914 (GRCm39)		probably null	Het
Gcm1	T	A	9: 77,971,816 (GRCm39)	D252E	probably benign	Het
Gm11559	A	G	11: 99,755,416 (GRCm39)	T22A	unknown	Het
Gm4847	C	T	1: 166,465,917 (GRCm39)	R224Q	probably damaging	Het
Hypk	A	G	2: 121,286,859 (GRCm39)		probably benign	Het
I830077J02Rik	C	T	3: 105,834,233 (GRCm39)	C64Y	possibly damaging	Het
Igkv4-86	T	C	6: 68,887,650 (GRCm39)	I30V	probably benign	Het
Il17ra	A	G	6: 120,455,416 (GRCm39)	I342V	probably benign	Het
Kcnip3	C	T	2: 127,352,828 (GRCm39)	A64T	possibly damaging	Het
Kdm5d	T	A	Y: 940,658 (GRCm39)	D1056E	possibly damaging	Het
Kif19b	C	T	5: 140,460,716 (GRCm39)	R461C	probably damaging	Het
Krtap19-2	C	T	16: 88,670,903 (GRCm39)	G81R	unknown	Het
Ldlrad1	G	A	4: 107,066,688 (GRCm39)	A8T	probably benign	Het
Lpin1	A	T	12: 16,629,965 (GRCm39)	I69N		Het
Matcap1	G	A	8: 106,012,145 (GRCm39)	R101W	probably benign	Het
Mki67	C	T	7: 135,299,293 (GRCm39)	D1914N	probably damaging	Het
Mmrn1	A	T	6: 60,937,707 (GRCm39)	Q235L	probably damaging	Het
Mndal	T	A	1: 173,699,111 (GRCm39)	K185*	probably null	Het
Muc6	T	C	7: 141,233,354 (GRCm39)	T802A	probably damaging	Het
Necab1	A	T	4: 15,005,073 (GRCm39)	F130L	probably damaging	Het
Nemf	A	T	12: 69,402,826 (GRCm39)	M70K	possibly damaging	Het
Nploc4	A	G	11: 120,280,240 (GRCm39)	I436T	possibly damaging	Het
Obscn	C	A	11: 59,015,439 (GRCm39)	R1011M	probably damaging	Het
Or4k44	A	T	2: 111,367,825 (GRCm39)	F270I	probably damaging	Het
Or5aq1b	T	C	2: 86,901,570 (GRCm39)	K303E	probably benign	Het
Or8k24	T	A	2: 86,215,911 (GRCm39)	M284L	probably benign	Het
Ormdl1	T	A	1: 53,338,139 (GRCm39)	M1K	probably null	Het
Pramel34	A	T	5: 93,784,597 (GRCm39)	L289Q	probably damaging	Het
Psmb3	A	G	11: 97,594,723 (GRCm39)	D38G	probably benign	Het
Psme4	T	A	11: 30,792,026 (GRCm39)	L1171Q	probably damaging	Het
Repin1	G	T	6: 48,574,279 (GRCm39)	E403*	probably null	Het
Slc22a18	G	A	7: 143,052,911 (GRCm39)	V379I	probably damaging	Het
Speg	T	A	1: 75,392,240 (GRCm39)	F1632L	probably damaging	Het
Taok1	G	A	11: 77,470,659 (GRCm39)	R49C	possibly damaging	Het
Ttll6	T	C	11: 96,047,425 (GRCm39)	S675P	probably benign	Het
Usp8	A	T	2: 126,559,918 (GRCm39)		probably benign	Het
Vmn2r81	T	A	10: 79,110,538 (GRCm39)	N550K	possibly damaging	Het
Zfp119a	A	T	17: 56,172,971 (GRCm39)	C291S	probably damaging	Het
Zfp329	C	A	7: 12,544,313 (GRCm39)	G404C	probably damaging	Het

Other mutations in Zfp934

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R1915:Zfp934	UTSW	13	62,665,769 (GRCm39)	missense	probably damaging	1.00
R1957:Zfp934	UTSW	13	62,666,108 (GRCm39)	missense	possibly damaging	0.83
R2113:Zfp934	UTSW	13	62,666,507 (GRCm39)	missense	probably damaging	0.98
R2145:Zfp934	UTSW	13	62,665,648 (GRCm39)	missense	probably damaging	1.00
R3732:Zfp934	UTSW	13	62,665,599 (GRCm39)	missense	probably damaging	1.00
R3797:Zfp934	UTSW	13	62,665,702 (GRCm39)	missense	probably benign
R4326:Zfp934	UTSW	13	62,665,373 (GRCm39)	missense	probably benign	0.01
R4327:Zfp934	UTSW	13	62,665,373 (GRCm39)	missense	probably benign	0.01
R4909:Zfp934	UTSW	13	62,665,768 (GRCm39)	missense	probably damaging	1.00
R5236:Zfp934	UTSW	13	62,665,527 (GRCm39)	missense	probably damaging	1.00
R5237:Zfp934	UTSW	13	62,665,652 (GRCm39)	missense	probably damaging	1.00
R6305:Zfp934	UTSW	13	62,666,370 (GRCm39)	missense	probably damaging	0.99
R6729:Zfp934	UTSW	13	62,640,746 (GRCm39)	missense	probably damaging	1.00
R7072:Zfp934	UTSW	13	62,668,339 (GRCm39)	missense	probably damaging	0.99
R7186:Zfp934	UTSW	13	62,640,204 (GRCm39)	missense	probably benign	0.12
R7235:Zfp934	UTSW	13	62,665,964 (GRCm39)	missense
R7453:Zfp934	UTSW	13	62,666,517 (GRCm39)	missense	probably benign	0.40
R7552:Zfp934	UTSW	13	62,640,705 (GRCm39)	missense	probably damaging	1.00
R7589:Zfp934	UTSW	13	62,666,130 (GRCm39)	missense
R7624:Zfp934	UTSW	13	62,666,338 (GRCm39)	missense	probably benign	0.27
R7651:Zfp934	UTSW	13	62,666,327 (GRCm39)	missense	probably benign
R7780:Zfp934	UTSW	13	62,666,358 (GRCm39)	missense	possibly damaging	0.92
R7857:Zfp934	UTSW	13	62,665,361 (GRCm39)	missense
R7891:Zfp934	UTSW	13	62,668,003 (GRCm39)	missense	probably benign	0.35
R8298:Zfp934	UTSW	13	62,666,295 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- GGATACACACTAAGGCATTGTTTCC -3'
(R):5'- CTCTACAAACATGTCATGCTGG -3'

Sequencing Primer
(F):5'- AAGCTGTACTAATTTACACTGCTCC -3'
(R):5'- CATGTCATGCTGGAGACCTACAG -3'

Posted On

2020-06-30