Incidental Mutation 'R8136:Wls'
ID 632249
Institutional Source Beutler Lab
Gene Symbol Wls
Ensembl Gene ENSMUSG00000028173
Gene Name wntless WNT ligand secretion mediator
Synonyms 5031439A09Rik, Gpr177
MMRRC Submission 067564-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R8136 (G1)
Quality Score 225.009
Status Validated
Chromosome 3
Chromosomal Location 159545309-159644300 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) C to A at 159578761 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glutamine to Lysine at position 108 (Q108K)
Ref Sequence ENSEMBL: ENSMUSP00000067898 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000068952] [ENSMUST00000198878] [ENSMUST00000200191]
AlphaFold Q6DID7
Predicted Effect probably damaging
Transcript: ENSMUST00000068952
AA Change: Q108K

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000067898
Gene: ENSMUSG00000028173
AA Change: Q108K

DomainStartEndE-ValueType
transmembrane domain 13 35 N/A INTRINSIC
low complexity region 36 47 N/A INTRINSIC
Pfam:MIG-14_Wnt-bd 178 496 3.7e-97 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000198878
AA Change: Q108K

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000143475
Gene: ENSMUSG00000028173
AA Change: Q108K

DomainStartEndE-ValueType
transmembrane domain 13 35 N/A INTRINSIC
low complexity region 36 47 N/A INTRINSIC
Pfam:MIG-14_Wnt-bd 177 497 2.8e-94 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000200191
Coding Region Coverage
  • 1x: 99.8%
  • 3x: 99.6%
  • 10x: 98.9%
  • 20x: 96.7%
Validation Efficiency 98% (40/41)
MGI Phenotype PHENOTYPE: Mice homozygous for null alleles exhibit impaired body axis and triploblastic development dying prior to E10.5. Mice homozygous for a floxed allele activated in keratinocytes exhibit a psoriasiform dermatitis-like phenotype. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 38 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca12 T A 1: 71,287,556 (GRCm39) M2462L probably benign Het
Aptx A G 4: 40,688,107 (GRCm39) probably null Het
Bmpr1b A G 3: 141,562,143 (GRCm39) I348T probably damaging Het
Btnl1 T A 17: 34,599,014 (GRCm39) probably null Het
Col27a1 T A 4: 63,202,190 (GRCm39) D960E probably benign Het
Crygn G T 5: 24,956,090 (GRCm39) R172S probably benign Het
Cyp26a1 T C 19: 37,689,654 (GRCm39) I450T probably benign Het
Emc2 A G 15: 43,375,202 (GRCm39) Y233C probably benign Het
Esyt2 C A 12: 116,327,079 (GRCm39) T549K probably benign Het
Garin5a T C 7: 44,149,704 (GRCm39) F142L probably damaging Het
Gatm T C 2: 122,426,018 (GRCm39) D411G probably damaging Het
Gpr65 T C 12: 98,241,415 (GRCm39) Y23H probably damaging Het
Itpr1 A G 6: 108,415,321 (GRCm39) R1752G probably benign Het
Krtap4-1 GGCAGCAGGGGCTGCAGCAAGGGCTGCAGCAGGGGC GGCAGCAGGGGCTGCAGCAAGGGCTGCAGCAGGGGCTGCAGCAAGGGCTGCAGCAGGGGC 11: 99,518,660 (GRCm39) probably benign Het
Map3k19 A C 1: 127,751,492 (GRCm39) S620A probably damaging Het
Mcm9 A G 10: 53,487,439 (GRCm39) *543Q probably null Het
Mfsd2a A T 4: 122,845,660 (GRCm39) C164S probably benign Het
Mta1 C A 12: 113,095,298 (GRCm39) R484S probably damaging Het
Nlrp9a T G 7: 26,256,678 (GRCm39) F99V probably benign Het
Notch2 G A 3: 98,031,537 (GRCm39) C1137Y probably damaging Het
Or51a6 A T 7: 102,604,448 (GRCm39) M120K probably damaging Het
Pcnx1 A G 12: 81,964,780 (GRCm39) T316A probably benign Het
Peds1 A C 2: 167,486,879 (GRCm39) Y167D probably benign Het
Phf11a A G 14: 59,515,018 (GRCm39) V221A probably benign Het
Pigb A G 9: 72,929,602 (GRCm39) L327P possibly damaging Het
Rab3c A T 13: 110,317,554 (GRCm39) Y110* probably null Het
Sdk2 G A 11: 113,742,539 (GRCm39) T790M probably damaging Het
Skic3 A G 13: 76,261,222 (GRCm39) K131R probably benign Het
Slc22a2 C T 17: 12,824,917 (GRCm39) P260S probably damaging Het
Sppl2a T C 2: 126,755,201 (GRCm39) probably null Het
Tnn T A 1: 159,934,630 (GRCm39) Q1261L probably damaging Het
Treml4 C A 17: 48,571,745 (GRCm39) Y49* probably null Het
Ubr3 T C 2: 69,851,523 (GRCm39) I1827T probably damaging Het
Vrtn C T 12: 84,696,809 (GRCm39) R520C probably damaging Het
Wipf1 G A 2: 73,267,879 (GRCm39) P173L possibly damaging Het
Zfp217 G A 2: 169,961,571 (GRCm39) S252F possibly damaging Het
Zfp39 A T 11: 58,782,228 (GRCm39) V178D probably damaging Het
Zfp777 C A 6: 48,021,559 (GRCm39) R21L probably benign Het
Other mutations in Wls
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01924:Wls APN 3 159,607,080 (GRCm39) nonsense probably null
IGL02065:Wls APN 3 159,616,993 (GRCm39) missense probably damaging 0.99
IGL02073:Wls APN 3 159,612,890 (GRCm39) critical splice donor site probably null
IGL02133:Wls APN 3 159,603,007 (GRCm39) missense probably damaging 1.00
R0374:Wls UTSW 3 159,603,074 (GRCm39) nonsense probably null
R0561:Wls UTSW 3 159,578,705 (GRCm39) missense probably benign 0.32
R1697:Wls UTSW 3 159,602,995 (GRCm39) missense probably benign 0.12
R1791:Wls UTSW 3 159,617,450 (GRCm39) missense probably benign 0.17
R2444:Wls UTSW 3 159,612,867 (GRCm39) missense probably damaging 1.00
R3161:Wls UTSW 3 159,603,073 (GRCm39) missense probably damaging 1.00
R4285:Wls UTSW 3 159,639,902 (GRCm39) missense probably benign
R4468:Wls UTSW 3 159,578,564 (GRCm39) missense probably damaging 0.96
R4472:Wls UTSW 3 159,603,020 (GRCm39) missense probably benign 0.01
R4782:Wls UTSW 3 159,603,082 (GRCm39) missense probably benign 0.03
R4799:Wls UTSW 3 159,603,082 (GRCm39) missense probably benign 0.03
R4809:Wls UTSW 3 159,603,082 (GRCm39) missense probably benign 0.03
R5006:Wls UTSW 3 159,617,428 (GRCm39) missense possibly damaging 0.68
R5212:Wls UTSW 3 159,578,645 (GRCm39) missense probably benign 0.15
R5434:Wls UTSW 3 159,639,976 (GRCm39) missense probably damaging 0.97
R5694:Wls UTSW 3 159,545,624 (GRCm39) missense probably benign 0.01
R6315:Wls UTSW 3 159,640,007 (GRCm39) critical splice donor site probably null
R7069:Wls UTSW 3 159,639,965 (GRCm39) missense probably damaging 1.00
R7243:Wls UTSW 3 159,615,402 (GRCm39) missense possibly damaging 0.49
R7529:Wls UTSW 3 159,578,644 (GRCm39) missense probably benign 0.43
R7697:Wls UTSW 3 159,616,955 (GRCm39) missense probably benign 0.21
R7842:Wls UTSW 3 159,578,816 (GRCm39) missense probably benign 0.09
R8536:Wls UTSW 3 159,578,748 (GRCm39) missense probably damaging 0.96
R8816:Wls UTSW 3 159,639,928 (GRCm39) missense possibly damaging 0.89
R9074:Wls UTSW 3 159,615,403 (GRCm39) missense possibly damaging 0.86
Predicted Primers PCR Primer
(F):5'- GGATGTCCGTAAGAACCACC -3'
(R):5'- CCTTTGCAGTCTTGGGTCTGAC -3'

Sequencing Primer
(F):5'- CCATAAAACAAGATGGCTGGC -3'
(R):5'- TCACCAGGATCCAAGCGATATATGTG -3'
Posted On 2020-06-30