Incidental Mutation 'R8136:Gpr65'
ID 632268
Institutional Source Beutler Lab
Gene Symbol Gpr65
Ensembl Gene ENSMUSG00000021886
Gene Name G-protein coupled receptor 65
Synonyms TDAG8, Dig1, Gpcr25
MMRRC Submission 067564-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R8136 (G1)
Quality Score 225.009
Status Validated
Chromosome 12
Chromosomal Location 98234894-98242903 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 98241415 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Tyrosine to Histidine at position 23 (Y23H)
Ref Sequence ENSEMBL: ENSMUSP00000074581 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000075072]
AlphaFold no structure available at present
Predicted Effect probably damaging
Transcript: ENSMUST00000075072
AA Change: Y23H

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000074581
Gene: ENSMUSG00000021886
AA Change: Y23H

DomainStartEndE-ValueType
Pfam:7tm_1 33 290 1.9e-41 PFAM
Coding Region Coverage
  • 1x: 99.8%
  • 3x: 99.6%
  • 10x: 98.9%
  • 20x: 96.7%
Validation Efficiency 98% (40/41)
MGI Phenotype PHENOTYPE: Homozygous mutant mice have thymocytes and splenocytes that are insensitive to pH-dependent cAMP production. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 38 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca12 T A 1: 71,287,556 (GRCm39) M2462L probably benign Het
Aptx A G 4: 40,688,107 (GRCm39) probably null Het
Bmpr1b A G 3: 141,562,143 (GRCm39) I348T probably damaging Het
Btnl1 T A 17: 34,599,014 (GRCm39) probably null Het
Col27a1 T A 4: 63,202,190 (GRCm39) D960E probably benign Het
Crygn G T 5: 24,956,090 (GRCm39) R172S probably benign Het
Cyp26a1 T C 19: 37,689,654 (GRCm39) I450T probably benign Het
Emc2 A G 15: 43,375,202 (GRCm39) Y233C probably benign Het
Esyt2 C A 12: 116,327,079 (GRCm39) T549K probably benign Het
Garin5a T C 7: 44,149,704 (GRCm39) F142L probably damaging Het
Gatm T C 2: 122,426,018 (GRCm39) D411G probably damaging Het
Itpr1 A G 6: 108,415,321 (GRCm39) R1752G probably benign Het
Krtap4-1 GGCAGCAGGGGCTGCAGCAAGGGCTGCAGCAGGGGC GGCAGCAGGGGCTGCAGCAAGGGCTGCAGCAGGGGCTGCAGCAAGGGCTGCAGCAGGGGC 11: 99,518,660 (GRCm39) probably benign Het
Map3k19 A C 1: 127,751,492 (GRCm39) S620A probably damaging Het
Mcm9 A G 10: 53,487,439 (GRCm39) *543Q probably null Het
Mfsd2a A T 4: 122,845,660 (GRCm39) C164S probably benign Het
Mta1 C A 12: 113,095,298 (GRCm39) R484S probably damaging Het
Nlrp9a T G 7: 26,256,678 (GRCm39) F99V probably benign Het
Notch2 G A 3: 98,031,537 (GRCm39) C1137Y probably damaging Het
Or51a6 A T 7: 102,604,448 (GRCm39) M120K probably damaging Het
Pcnx1 A G 12: 81,964,780 (GRCm39) T316A probably benign Het
Peds1 A C 2: 167,486,879 (GRCm39) Y167D probably benign Het
Phf11a A G 14: 59,515,018 (GRCm39) V221A probably benign Het
Pigb A G 9: 72,929,602 (GRCm39) L327P possibly damaging Het
Rab3c A T 13: 110,317,554 (GRCm39) Y110* probably null Het
Sdk2 G A 11: 113,742,539 (GRCm39) T790M probably damaging Het
Skic3 A G 13: 76,261,222 (GRCm39) K131R probably benign Het
Slc22a2 C T 17: 12,824,917 (GRCm39) P260S probably damaging Het
Sppl2a T C 2: 126,755,201 (GRCm39) probably null Het
Tnn T A 1: 159,934,630 (GRCm39) Q1261L probably damaging Het
Treml4 C A 17: 48,571,745 (GRCm39) Y49* probably null Het
Ubr3 T C 2: 69,851,523 (GRCm39) I1827T probably damaging Het
Vrtn C T 12: 84,696,809 (GRCm39) R520C probably damaging Het
Wipf1 G A 2: 73,267,879 (GRCm39) P173L possibly damaging Het
Wls C A 3: 159,578,761 (GRCm39) Q108K probably damaging Het
Zfp217 G A 2: 169,961,571 (GRCm39) S252F possibly damaging Het
Zfp39 A T 11: 58,782,228 (GRCm39) V178D probably damaging Het
Zfp777 C A 6: 48,021,559 (GRCm39) R21L probably benign Het
Other mutations in Gpr65
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00435:Gpr65 APN 12 98,241,815 (GRCm39) missense probably damaging 1.00
IGL00717:Gpr65 APN 12 98,242,314 (GRCm39) missense probably benign 0.09
IGL01643:Gpr65 APN 12 98,242,013 (GRCm39) missense probably damaging 1.00
IGL01942:Gpr65 APN 12 98,241,974 (GRCm39) missense possibly damaging 0.93
IGL02023:Gpr65 APN 12 98,242,127 (GRCm39) missense probably benign 0.25
IGL02803:Gpr65 APN 12 98,241,469 (GRCm39) missense probably damaging 1.00
R1343:Gpr65 UTSW 12 98,241,888 (GRCm39) missense probably benign 0.00
R1520:Gpr65 UTSW 12 98,241,434 (GRCm39) missense probably benign 0.01
R1771:Gpr65 UTSW 12 98,242,259 (GRCm39) missense probably damaging 0.96
R1812:Gpr65 UTSW 12 98,242,001 (GRCm39) missense probably damaging 1.00
R2261:Gpr65 UTSW 12 98,241,494 (GRCm39) missense probably damaging 1.00
R2263:Gpr65 UTSW 12 98,241,494 (GRCm39) missense probably damaging 1.00
R5720:Gpr65 UTSW 12 98,241,361 (GRCm39) missense probably damaging 1.00
R7458:Gpr65 UTSW 12 98,242,324 (GRCm39) missense probably damaging 0.99
R9376:Gpr65 UTSW 12 98,241,523 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- ACCACTCTCTAAATGCATGGATG -3'
(R):5'- CTTTGCACAAGGTGGGAGAG -3'

Sequencing Primer
(F):5'- CACTCTCTAAATGCATGGATGAACTC -3'
(R):5'- GAGAAAGTCCAGTTGTCTTTATTCC -3'
Posted On 2020-06-30