Mutagenetix > Incidental Mutation

Incidental Mutation 'R8142:Zbtb34'

632570

Institutional Source

Beutler Lab

Gene Symbol

Zbtb34

Ensembl Gene

ENSMUSG00000068966

Gene Name

zinc finger and BTB domain containing 34

Synonyms

LOC241311

MMRRC Submission

067570-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.262) question?

Stock #

R8142 (G1)

Quality Score

201.009

Status

Not validated

Chromosome

Chromosomal Location

33296120-33321336 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to T at 33302493 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Stop codon at position 16 (S16*)

Ref Sequence

ENSEMBL: ENSMUSP00000088560 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091037] [ENSMUST00000113158]

AlphaFold

A2ATY4

Predicted Effect

probably null
Transcript: ENSMUST00000091037
AA Change: S16*

SMART Domains

Protein: ENSMUSP00000088560
Gene: ENSMUSG00000068966
AA Change: S16*

Domain	Start	End	E-Value	Type
BTB	50	144	1.28e-22	SMART
low complexity region	259	273	N/A	INTRINSIC
ZnF_C2H2	390	412	1.06e-4	SMART
ZnF_C2H2	418	440	1.12e-3	SMART
ZnF_C2H2	446	469	1.69e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000113158
AA Change: S16*

SMART Domains

Protein: ENSMUSP00000108783
Gene: ENSMUSG00000068966
AA Change: S16*

Domain	Start	End	E-Value	Type
BTB	50	144	1.28e-22	SMART
low complexity region	259	273	N/A	INTRINSIC
ZnF_C2H2	390	412	1.06e-4	SMART
ZnF_C2H2	418	440	1.12e-3	SMART
ZnF_C2H2	446	469	1.69e-3	SMART

Coding Region Coverage

1x: 99.7%
3x: 99.5%
10x: 98.0%
20x: 91.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Atp6v1e2	A	T	17: 87,252,083 (GRCm39)	I105N	possibly damaging	Het
Azi2	A	G	9: 117,878,475 (GRCm39)	D105G	probably damaging	Het
Bmpr2	T	C	1: 59,909,465 (GRCm39)	S980P	probably damaging	Het
Ccno	T	G	13: 113,125,489 (GRCm39)	L151R	probably damaging	Het
Cdh2	A	C	18: 16,734,791 (GRCm39)	I801S	probably benign	Het
Cyp2f2	G	A	7: 26,828,678 (GRCm39)	V183I	probably benign	Het
Dab1	T	A	4: 104,535,921 (GRCm39)	V110D	probably damaging	Het
Dnah3	T	C	7: 119,660,189 (GRCm39)	T828A	probably benign	Het
Dnah5	G	T	15: 28,384,519 (GRCm39)	V3088L	probably benign	Het
Dtd2	T	A	12: 52,046,593 (GRCm39)	D82V	probably damaging	Het
Dusp5	T	C	19: 53,525,912 (GRCm39)	F185L	probably damaging	Het
Epha10	A	G	4: 124,779,639 (GRCm39)	T162A	probably damaging	Het
Fat4	A	T	3: 38,945,352 (GRCm39)	D1415V	probably damaging	Het
Fitm1	T	C	14: 55,813,247 (GRCm39)	Y37H	possibly damaging	Het
Flg2	G	T	3: 93,122,782 (GRCm39)	E1651*	probably null	Het
Ifit3	G	T	19: 34,564,901 (GRCm39)	C149F	probably damaging	Het
Lepr	C	A	4: 101,622,616 (GRCm39)	H465Q	possibly damaging	Het
Ltn1	A	C	16: 87,178,529 (GRCm39)	S1567A	probably benign	Het
Marchf1	T	C	8: 66,908,778 (GRCm39)	V166A	probably benign	Het
Marveld2	T	C	13: 100,737,448 (GRCm39)	H424R	possibly damaging	Het
Mypop	A	G	7: 18,735,051 (GRCm39)	T383A	unknown	Het
Ngef	G	C	1: 87,468,463 (GRCm39)	R99G	probably benign	Het
Nherf4	A	G	9: 44,162,078 (GRCm39)		probably null	Het
Npepps	G	T	11: 97,109,398 (GRCm39)	A726D	probably damaging	Het
Or6c63-ps1	T	A	10: 128,900,519 (GRCm39)	D9V	probably benign	Het
Pcdhgb4	A	G	18: 37,854,166 (GRCm39)	D187G	probably damaging	Het
Per2	T	C	1: 91,349,269 (GRCm39)	E1034G	possibly damaging	Het
Pira1	A	G	7: 3,739,842 (GRCm39)	S418P	possibly damaging	Het
Pkhd1l1	G	A	15: 44,378,327 (GRCm39)	R1027H	probably benign	Het
Pon2	A	T	6: 5,266,239 (GRCm39)	V260D	probably benign	Het
Rnase13	A	G	14: 52,159,893 (GRCm39)	I82T	probably damaging	Het
Sbno1	A	G	5: 124,546,608 (GRCm39)	S194P	probably benign	Het
Serpinb6c	T	A	13: 34,064,096 (GRCm39)	I320L	probably benign	Het
Sh3rf3	C	T	10: 58,885,205 (GRCm39)	R363*	probably null	Het
Slc11a1	T	C	1: 74,424,418 (GRCm39)	F500L	probably benign	Het
Slc1a4	A	G	11: 20,257,890 (GRCm39)		probably null	Het
Slc1a7	G	A	4: 107,869,473 (GRCm39)	V513M	probably benign	Het
Sorcs2	T	C	5: 36,219,958 (GRCm39)	N362S	possibly damaging	Het
Stau2	A	G	1: 16,530,575 (GRCm39)	S115P	unknown	Het
Stk38l	A	G	6: 146,660,070 (GRCm39)	N34S	probably benign	Het
Tmem201	T	C	4: 149,803,114 (GRCm39)	T585A	probably benign	Het
Ttc6	A	T	12: 57,744,258 (GRCm39)	I1297F	possibly damaging	Het
Uncx	A	G	5: 139,532,655 (GRCm39)	D240G	possibly damaging	Het
Vmn2r88	A	G	14: 51,651,564 (GRCm39)	I293V		Het
Vps11	T	C	9: 44,265,852 (GRCm39)	T476A	probably benign	Het
Vrtn	T	C	12: 84,697,395 (GRCm39)	L715P	probably damaging	Het
Wdr72	T	G	9: 74,046,949 (GRCm39)	V65G	probably damaging	Het
Zbtb21	T	C	16: 97,752,675 (GRCm39)	E536G	probably damaging	Het
Zfhx2	G	A	14: 55,310,895 (GRCm39)	L600F	possibly damaging	Het

Other mutations in Zbtb34

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02721:Zbtb34	APN	2	33,301,270 (GRCm39)	missense	probably damaging	1.00
R0398:Zbtb34	UTSW	2	33,301,060 (GRCm39)	nonsense	probably null
R1767:Zbtb34	UTSW	2	33,301,348 (GRCm39)	missense	possibly damaging	0.95
R4973:Zbtb34	UTSW	2	33,301,626 (GRCm39)	missense	probably benign	0.28
R5150:Zbtb34	UTSW	2	33,301,133 (GRCm39)	missense	probably damaging	1.00
R6063:Zbtb34	UTSW	2	33,301,842 (GRCm39)	missense	possibly damaging	0.75
R6489:Zbtb34	UTSW	2	33,301,558 (GRCm39)	missense	probably damaging	1.00
R6525:Zbtb34	UTSW	2	33,302,145 (GRCm39)	missense	probably damaging	0.97
R7973:Zbtb34	UTSW	2	33,301,531 (GRCm39)	missense	probably benign
R8909:Zbtb34	UTSW	2	33,301,701 (GRCm39)	missense	possibly damaging	0.57
R8978:Zbtb34	UTSW	2	33,301,048 (GRCm39)	missense	possibly damaging	0.92
R9038:Zbtb34	UTSW	2	33,301,248 (GRCm39)	missense	probably damaging	1.00
R9337:Zbtb34	UTSW	2	33,301,716 (GRCm39)	missense	probably damaging	1.00
R9727:Zbtb34	UTSW	2	33,301,533 (GRCm39)	missense	probably benign
Z1088:Zbtb34	UTSW	2	33,301,120 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTGCTGAATGGTCCCGGAAATAC -3'
(R):5'- TGATGCTGGGCCAATGGATC -3'

Sequencing Primer
(F):5'- AATACGGGGAGCTGGCTGC -3'
(R):5'- TGGGCCAATGGATCATTCAC -3'

Posted On

2020-06-30