Incidental Mutation 'R8142:Dusp5'
ID 632614
Institutional Source Beutler Lab
Gene Symbol Dusp5
Ensembl Gene ENSMUSG00000034765
Gene Name dual specificity phosphatase 5
Synonyms LOC240672
MMRRC Submission 067570-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R8142 (G1)
Quality Score 194.009
Status Not validated
Chromosome 19
Chromosomal Location 53517576-53530240 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 53525912 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Phenylalanine to Leucine at position 185 (F185L)
Ref Sequence ENSEMBL: ENSMUSP00000047900 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000038287]
AlphaFold Q1HL35
Predicted Effect probably damaging
Transcript: ENSMUST00000038287
AA Change: F185L

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000047900
Gene: ENSMUSG00000034765
AA Change: F185L

DomainStartEndE-ValueType
RHOD 9 138 1.88e-13 SMART
DSPc 178 316 4.48e-56 SMART
Coding Region Coverage
  • 1x: 99.7%
  • 3x: 99.5%
  • 10x: 98.0%
  • 20x: 91.7%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased proliferation and apoptosis and altered metabolic profiles in T cells. Mice homozygous for other null alleles exhibit altered eosinophilic response to parasicitc infection and increased susceptibility to induced tumors. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 49 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Atp6v1e2 A T 17: 87,252,083 (GRCm39) I105N possibly damaging Het
Azi2 A G 9: 117,878,475 (GRCm39) D105G probably damaging Het
Bmpr2 T C 1: 59,909,465 (GRCm39) S980P probably damaging Het
Ccno T G 13: 113,125,489 (GRCm39) L151R probably damaging Het
Cdh2 A C 18: 16,734,791 (GRCm39) I801S probably benign Het
Cyp2f2 G A 7: 26,828,678 (GRCm39) V183I probably benign Het
Dab1 T A 4: 104,535,921 (GRCm39) V110D probably damaging Het
Dnah3 T C 7: 119,660,189 (GRCm39) T828A probably benign Het
Dnah5 G T 15: 28,384,519 (GRCm39) V3088L probably benign Het
Dtd2 T A 12: 52,046,593 (GRCm39) D82V probably damaging Het
Epha10 A G 4: 124,779,639 (GRCm39) T162A probably damaging Het
Fat4 A T 3: 38,945,352 (GRCm39) D1415V probably damaging Het
Fitm1 T C 14: 55,813,247 (GRCm39) Y37H possibly damaging Het
Flg2 G T 3: 93,122,782 (GRCm39) E1651* probably null Het
Ifit3 G T 19: 34,564,901 (GRCm39) C149F probably damaging Het
Lepr C A 4: 101,622,616 (GRCm39) H465Q possibly damaging Het
Ltn1 A C 16: 87,178,529 (GRCm39) S1567A probably benign Het
Marchf1 T C 8: 66,908,778 (GRCm39) V166A probably benign Het
Marveld2 T C 13: 100,737,448 (GRCm39) H424R possibly damaging Het
Mypop A G 7: 18,735,051 (GRCm39) T383A unknown Het
Ngef G C 1: 87,468,463 (GRCm39) R99G probably benign Het
Nherf4 A G 9: 44,162,078 (GRCm39) probably null Het
Npepps G T 11: 97,109,398 (GRCm39) A726D probably damaging Het
Or6c63-ps1 T A 10: 128,900,519 (GRCm39) D9V probably benign Het
Pcdhgb4 A G 18: 37,854,166 (GRCm39) D187G probably damaging Het
Per2 T C 1: 91,349,269 (GRCm39) E1034G possibly damaging Het
Pira1 A G 7: 3,739,842 (GRCm39) S418P possibly damaging Het
Pkhd1l1 G A 15: 44,378,327 (GRCm39) R1027H probably benign Het
Pon2 A T 6: 5,266,239 (GRCm39) V260D probably benign Het
Rnase13 A G 14: 52,159,893 (GRCm39) I82T probably damaging Het
Sbno1 A G 5: 124,546,608 (GRCm39) S194P probably benign Het
Serpinb6c T A 13: 34,064,096 (GRCm39) I320L probably benign Het
Sh3rf3 C T 10: 58,885,205 (GRCm39) R363* probably null Het
Slc11a1 T C 1: 74,424,418 (GRCm39) F500L probably benign Het
Slc1a4 A G 11: 20,257,890 (GRCm39) probably null Het
Slc1a7 G A 4: 107,869,473 (GRCm39) V513M probably benign Het
Sorcs2 T C 5: 36,219,958 (GRCm39) N362S possibly damaging Het
Stau2 A G 1: 16,530,575 (GRCm39) S115P unknown Het
Stk38l A G 6: 146,660,070 (GRCm39) N34S probably benign Het
Tmem201 T C 4: 149,803,114 (GRCm39) T585A probably benign Het
Ttc6 A T 12: 57,744,258 (GRCm39) I1297F possibly damaging Het
Uncx A G 5: 139,532,655 (GRCm39) D240G possibly damaging Het
Vmn2r88 A G 14: 51,651,564 (GRCm39) I293V Het
Vps11 T C 9: 44,265,852 (GRCm39) T476A probably benign Het
Vrtn T C 12: 84,697,395 (GRCm39) L715P probably damaging Het
Wdr72 T G 9: 74,046,949 (GRCm39) V65G probably damaging Het
Zbtb21 T C 16: 97,752,675 (GRCm39) E536G probably damaging Het
Zbtb34 G T 2: 33,302,493 (GRCm39) S16* probably null Het
Zfhx2 G A 14: 55,310,895 (GRCm39) L600F possibly damaging Het
Other mutations in Dusp5
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01949:Dusp5 APN 19 53,525,904 (GRCm39) missense probably damaging 1.00
IGL02017:Dusp5 APN 19 53,525,937 (GRCm39) missense probably damaging 1.00
R4523:Dusp5 UTSW 19 53,526,032 (GRCm39) missense probably damaging 1.00
R5350:Dusp5 UTSW 19 53,529,665 (GRCm39) missense probably damaging 1.00
R7941:Dusp5 UTSW 19 53,525,964 (GRCm39) missense probably benign
R8021:Dusp5 UTSW 19 53,517,929 (GRCm39) missense probably benign 0.13
R8155:Dusp5 UTSW 19 53,529,537 (GRCm39) nonsense probably null
R8336:Dusp5 UTSW 19 53,529,406 (GRCm39) missense probably damaging 1.00
R8353:Dusp5 UTSW 19 53,518,113 (GRCm39) missense possibly damaging 0.48
R8881:Dusp5 UTSW 19 53,529,745 (GRCm39) missense probably benign 0.05
R9640:Dusp5 UTSW 19 53,526,051 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TCACTGAGTCATGAGGGTAGAG -3'
(R):5'- TGGGAGCTAATGTCAGCAGTG -3'

Sequencing Primer
(F):5'- AGTGCCAGGGTGCAGGTAC -3'
(R):5'- AGCAGTGTGGCTGTCCTC -3'
Posted On 2020-06-30