Mutagenetix > Incidental Mutation

Incidental Mutation 'R8143:Or7g34'

632645

Institutional Source

Beutler Lab

Gene Symbol

Or7g34

Ensembl Gene

ENSMUSG00000095667

Gene Name

olfactory receptor family 7 subfamily G member 34

Synonyms

MOR147-3, GA_x6K02T2PVTD-13313295-13312357, Olfr854

MMRRC Submission

067571-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.419) question?

Stock #

R8143 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19477731-19478678 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 19478587 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 31 (V31A)

Ref Sequence

ENSEMBL: ENSMUSP00000072248 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072419] [ENSMUST00000212872]

AlphaFold

Q8VFF1

Predicted Effect

probably benign
Transcript: ENSMUST00000072419
AA Change: V31A

PolyPhen 2 Score 0.036 (Sensitivity: 0.94; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000072248
Gene: ENSMUSG00000095667
AA Change: V31A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	34	311	2.3e-51	PFAM
Pfam:7TM_GPCR_Srsx	38	308	9.6e-7	PFAM
Pfam:7tm_1	44	293	1.9e-23	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000212872
AA Change: V28A

PolyPhen 2 Score 0.036 (Sensitivity: 0.94; Specificity: 0.82)

Coding Region Coverage

1x: 99.7%
3x: 99.4%
10x: 97.3%
20x: 85.9%

Validation Efficiency

100% (65/65)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930402F06Rik	C	A	2: 35,265,884 (GRCm39)	C262F	probably damaging	Het
Acss1	T	C	2: 150,509,801 (GRCm39)		probably null	Het
Adamtsl1	T	C	4: 86,260,492 (GRCm39)	V909A	possibly damaging	Het
Adprh	G	A	16: 38,270,694 (GRCm39)	T37M	probably benign	Het
Ak9	T	A	10: 41,213,588 (GRCm39)	Y264*	probably null	Het
Aox3	G	T	1: 58,198,074 (GRCm39)	A629S	probably benign	Het
Apcs	G	A	1: 172,721,900 (GRCm39)	P149S	probably damaging	Het
Arnt	T	A	3: 95,377,294 (GRCm39)		probably null	Het
Asb13	G	A	13: 3,692,065 (GRCm39)	G15D	probably damaging	Het
Bltp1	T	G	3: 37,000,657 (GRCm39)		probably null	Het
Cacnb1	T	G	11: 97,894,146 (GRCm39)	T459P	probably benign	Het
Chrnb2	T	C	3: 89,654,630 (GRCm39)	T718A	unknown	Het
Cttn	T	A	7: 144,014,999 (GRCm39)	K70*	probably null	Het
Cyp2f2	G	A	7: 26,828,678 (GRCm39)	V183I	probably benign	Het
Cyp39a1	T	C	17: 44,036,517 (GRCm39)	V349A	probably benign	Het
Ebf2	C	A	14: 67,649,386 (GRCm39)	Y430*	probably null	Het
Eef2	T	C	10: 81,017,182 (GRCm39)	Y730H	probably damaging	Het
Eif2ak2	G	A	17: 79,165,961 (GRCm39)	T412I	probably benign	Het
Flnc	G	C	6: 29,441,484 (GRCm39)	R422P	probably benign	Het
Fryl	G	A	5: 73,207,682 (GRCm39)	A2496V	probably benign	Het
Galnt6	A	G	15: 100,614,088 (GRCm39)	L71P	probably damaging	Het
Gm7579	C	T	7: 141,766,163 (GRCm39)	Q190*	probably null	Het
H2-T3	T	A	17: 36,498,384 (GRCm39)	R220S	probably benign	Het
Hmcn1	A	G	1: 150,734,957 (GRCm39)	V185A	probably benign	Het
Ifi208	T	A	1: 173,510,242 (GRCm39)	D132E	possibly damaging	Het
Itgb4	G	A	11: 115,884,255 (GRCm39)	V981I	probably damaging	Het
Itsn2	A	G	12: 4,683,003 (GRCm39)	T310A	unknown	Het
Kat5	A	G	19: 5,657,577 (GRCm39)		probably null	Het
Kif22	A	T	7: 126,632,397 (GRCm39)	D304E	probably damaging	Het
Mapre3	A	T	5: 31,020,719 (GRCm39)	N147Y	possibly damaging	Het
Morc2a	T	C	11: 3,628,537 (GRCm39)	V330A	probably benign	Het
Mylk	G	A	16: 34,734,525 (GRCm39)	V709M	possibly damaging	Het
Myo3a	T	G	2: 22,287,476 (GRCm39)		probably null	Het
N4bp2l2	A	T	5: 150,585,670 (GRCm39)	D103E	probably benign	Het
Nckap1	T	C	2: 80,336,530 (GRCm39)	K1062R	possibly damaging	Het
Nol9	T	C	4: 152,125,559 (GRCm39)	V170A	possibly damaging	Het
Numa1	A	G	7: 101,648,891 (GRCm39)	Q874R	possibly damaging	Het
Olig3	T	G	10: 19,232,732 (GRCm39)	V119G	probably damaging	Het
Osbpl9	A	G	4: 108,922,906 (GRCm39)	S485P	probably benign	Het
Otogl	A	G	10: 107,642,527 (GRCm39)	F1301S	probably damaging	Het
Pank3	A	G	11: 35,667,036 (GRCm39)	Y51C	probably damaging	Het
Pex5l	T	C	3: 33,136,658 (GRCm39)		probably benign	Het
Pgm1	G	A	4: 99,824,415 (GRCm39)		probably null	Het
Psmd9	T	G	5: 123,366,479 (GRCm39)	I37S	probably damaging	Het
Qtrt2	A	G	16: 43,692,117 (GRCm39)	S135P	probably damaging	Het
Rexo5	T	A	7: 119,433,484 (GRCm39)		probably null	Het
Rsrp1	T	A	4: 134,654,319 (GRCm39)	D286E	probably benign	Het
Scara3	C	T	14: 66,169,269 (GRCm39)	C116Y	probably damaging	Het
Serpina3b	A	T	12: 104,096,793 (GRCm39)	T25S	probably benign	Het
Sh3pxd2a	G	A	19: 47,257,138 (GRCm39)	P555S	probably damaging	Het
Slc1a2	T	A	2: 102,568,230 (GRCm39)	L89Q	probably damaging	Het
Tgm6	T	A	2: 129,983,763 (GRCm39)	N307K	probably damaging	Het
Tmem86b	G	T	7: 4,631,484 (GRCm39)	D189E	probably damaging	Het
Trim65	G	A	11: 116,017,287 (GRCm39)	R392C	probably benign	Het
Trpc7	T	C	13: 56,930,362 (GRCm39)	T769A	probably benign	Het
Trrap	T	A	5: 144,772,707 (GRCm39)		probably null	Het
Ttn	T	C	2: 76,693,677 (GRCm39)	E397G	possibly damaging	Het
Vcl	A	T	14: 21,037,112 (GRCm39)	M237L	possibly damaging	Het
Vmn1r36	G	A	6: 66,693,036 (GRCm39)	Q280*	probably null	Het
Vmn2r23	A	T	6: 123,718,312 (GRCm39)	D555V	probably damaging	Het
Wnk4	A	G	11: 101,153,625 (GRCm39)	N229S	probably damaging	Het
Zfp784	A	C	7: 5,038,910 (GRCm39)	V216G	possibly damaging	Het

Other mutations in Or7g34

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00930:Or7g34	APN	9	19,478,471 (GRCm39)	missense	probably damaging	1.00
IGL02072:Or7g34	APN	9	19,478,245 (GRCm39)	missense	probably benign	0.00
IGL02156:Or7g34	APN	9	19,478,494 (GRCm39)	missense	probably damaging	1.00
IGL02187:Or7g34	APN	9	19,478,393 (GRCm39)	missense	probably benign	0.16
IGL02252:Or7g34	APN	9	19,478,267 (GRCm39)	missense	probably damaging	0.99
R0519:Or7g34	UTSW	9	19,478,245 (GRCm39)	missense	probably benign	0.21
R0755:Or7g34	UTSW	9	19,478,415 (GRCm39)	missense	possibly damaging	0.94
R0899:Or7g34	UTSW	9	19,477,843 (GRCm39)	missense	probably damaging	1.00
R1832:Or7g34	UTSW	9	19,478,492 (GRCm39)	missense	possibly damaging	0.88
R2115:Or7g34	UTSW	9	19,478,618 (GRCm39)	missense	probably benign	0.12
R4326:Or7g34	UTSW	9	19,478,318 (GRCm39)	missense	possibly damaging	0.95
R4328:Or7g34	UTSW	9	19,478,318 (GRCm39)	missense	possibly damaging	0.95
R4329:Or7g34	UTSW	9	19,478,318 (GRCm39)	missense	possibly damaging	0.95
R4379:Or7g34	UTSW	9	19,478,038 (GRCm39)	missense	probably benign	0.15
R4381:Or7g34	UTSW	9	19,478,038 (GRCm39)	missense	probably benign	0.15
R5576:Or7g34	UTSW	9	19,478,369 (GRCm39)	missense	probably benign
R6102:Or7g34	UTSW	9	19,478,318 (GRCm39)	missense	possibly damaging	0.87
R7449:Or7g34	UTSW	9	19,478,162 (GRCm39)	missense	probably benign	0.06
R7515:Or7g34	UTSW	9	19,477,949 (GRCm39)	missense	probably damaging	1.00
R8813:Or7g34	UTSW	9	19,477,895 (GRCm39)	missense	probably damaging	1.00
R9515:Or7g34	UTSW	9	19,478,396 (GRCm39)	missense	possibly damaging	0.74
Z1176:Or7g34	UTSW	9	19,477,822 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CATTTGAGTGAGGCAGCCTTTG -3'
(R):5'- TCAGGGATATTATTCCAGCAACTTC -3'

Sequencing Primer
(F):5'- AGGCAGCCTTTGTATGTGATGC -3'
(R):5'- CAGCAACTTCTTAAGCTGTGACAG -3'

Posted On

2020-06-30