Incidental Mutation 'R8147:Adgrg7'
| ID |
632884 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Adgrg7
|
| Ensembl Gene |
ENSMUSG00000022755 |
| Gene Name |
adhesion G protein-coupled receptor G7 |
| Synonyms |
9130020O16Rik, Gpr128 |
| MMRRC Submission |
067575-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R8147 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
16 |
| Chromosomal Location |
56544972-56616218 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to C
at 56562876 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Glutamic Acid to Glycine
at position 487
(E487G)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000023437
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000023437]
|
| AlphaFold |
Q8BM96 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000023437
AA Change: E487G
PolyPhen 2
Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
|
| SMART Domains |
Protein: ENSMUSP00000023437
Gene: ENSMUSG00000022755
AA Change: E487G
| Domain | Start | End | E-Value | Type |
|---|
|
transmembrane domain
|
12 |
34 |
N/A |
INTRINSIC |
|
SCOP:d1edmb_
|
52 |
76 |
1e-3 |
SMART |
|
GPS
|
376 |
424 |
6.16e-8 |
SMART |
|
Pfam:7tm_2
|
428 |
712 |
4.5e-40 |
PFAM |
|
| Coding Region Coverage |
- 1x: 99.8%
- 3x: 99.5%
- 10x: 98.5%
- 20x: 94.3%
|
| Validation Efficiency |
100% (55/55) |
| MGI Phenotype |
PHENOTYPE: Mice homozygous for a knock-out allele exhibit reduced body weight gain and increased frequency of peristalsis and slow wave potential in the small intestine. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 55 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Aldh9a1 |
T |
A |
1: 167,184,949 (GRCm39) |
I287N |
probably damaging |
Het |
| Aox1 |
T |
A |
1: 58,339,821 (GRCm39) |
N371K |
probably benign |
Het |
| Atr |
T |
A |
9: 95,781,113 (GRCm39) |
F1392L |
probably damaging |
Het |
| Bicra |
G |
A |
7: 15,722,395 (GRCm39) |
P374L |
possibly damaging |
Het |
| Cacna1b |
C |
T |
2: 24,569,188 (GRCm39) |
R912Q |
probably damaging |
Het |
| Cdhr1 |
T |
C |
14: 36,801,609 (GRCm39) |
N778S |
probably benign |
Het |
| Cert1 |
C |
T |
13: 96,679,736 (GRCm39) |
P22L |
probably benign |
Het |
| Dgat2 |
C |
T |
7: 98,806,187 (GRCm39) |
V337I |
possibly damaging |
Het |
| Dlg5 |
T |
C |
14: 24,208,395 (GRCm39) |
D937G |
probably benign |
Het |
| Dqx1 |
T |
C |
6: 83,037,222 (GRCm39) |
V295A |
probably benign |
Het |
| Dsg3 |
A |
G |
18: 20,673,130 (GRCm39) |
T934A |
probably benign |
Het |
| Fam81b |
A |
C |
13: 76,383,140 (GRCm39) |
V243G |
probably damaging |
Het |
| Gen1 |
A |
T |
12: 11,305,051 (GRCm39) |
|
probably null |
Het |
| Glrx3 |
G |
A |
7: 137,064,736 (GRCm39) |
S275N |
probably benign |
Het |
| Igdcc4 |
A |
T |
9: 65,031,253 (GRCm39) |
Q397L |
probably benign |
Het |
| Ints6 |
C |
T |
14: 62,951,186 (GRCm39) |
G281D |
probably damaging |
Het |
| Kalrn |
A |
T |
16: 33,875,414 (GRCm39) |
S1722T |
probably benign |
Het |
| Lrrc4b |
A |
G |
7: 44,111,829 (GRCm39) |
D567G |
probably damaging |
Het |
| Macf1 |
A |
G |
4: 123,385,491 (GRCm39) |
L1200S |
probably damaging |
Het |
| Mad1l1 |
G |
A |
5: 140,129,734 (GRCm39) |
R434C |
probably damaging |
Het |
| Mia3 |
A |
T |
1: 183,109,062 (GRCm39) |
F358I |
|
Het |
| Ncapd3 |
C |
T |
9: 26,942,014 (GRCm39) |
|
probably benign |
Het |
| Nfkb2 |
A |
G |
19: 46,295,873 (GRCm39) |
R156G |
possibly damaging |
Het |
| Nus1 |
T |
C |
10: 52,305,416 (GRCm39) |
|
probably null |
Het |
| Or2y14 |
C |
T |
11: 49,405,050 (GRCm39) |
T195I |
probably benign |
Het |
| Or4a79 |
T |
A |
2: 89,552,186 (GRCm39) |
I90F |
probably damaging |
Het |
| Or4c31 |
T |
C |
2: 88,292,427 (GRCm39) |
F267L |
possibly damaging |
Het |
| Or5aq1b |
T |
A |
2: 86,902,017 (GRCm39) |
I154F |
probably benign |
Het |
| Pafah1b2 |
A |
G |
9: 45,895,147 (GRCm39) |
|
probably null |
Het |
| Pold2 |
T |
C |
11: 5,826,842 (GRCm39) |
T31A |
probably benign |
Het |
| Ppp1r12b |
T |
C |
1: 134,801,680 (GRCm39) |
N512S |
possibly damaging |
Het |
| Prl8a8 |
A |
T |
13: 27,695,564 (GRCm39) |
L23Q |
probably damaging |
Het |
| Ptprb |
T |
C |
10: 116,153,283 (GRCm39) |
V293A |
probably damaging |
Het |
| Rpl18a |
G |
T |
8: 71,348,069 (GRCm39) |
T174N |
probably damaging |
Het |
| Senp5 |
A |
T |
16: 31,808,128 (GRCm39) |
H375Q |
probably benign |
Het |
| Setbp1 |
A |
T |
18: 78,900,015 (GRCm39) |
F1217L |
probably damaging |
Het |
| Skint4 |
A |
G |
4: 111,993,218 (GRCm39) |
I314V |
probably benign |
Het |
| Slc28a2 |
A |
G |
2: 122,288,682 (GRCm39) |
D596G |
probably benign |
Het |
| Stat5b |
A |
G |
11: 100,688,607 (GRCm39) |
I285T |
probably benign |
Het |
| Sycp3 |
T |
C |
10: 88,298,467 (GRCm39) |
|
probably null |
Het |
| Sympk |
T |
C |
7: 18,770,718 (GRCm39) |
F270L |
probably damaging |
Het |
| Taar5 |
T |
C |
10: 23,846,749 (GRCm39) |
V49A |
probably damaging |
Het |
| Tbx18 |
C |
T |
9: 87,606,411 (GRCm39) |
V245I |
probably damaging |
Het |
| Tet1 |
A |
G |
10: 62,714,586 (GRCm39) |
M403T |
probably benign |
Het |
| Tmprss11f |
A |
T |
5: 86,677,769 (GRCm39) |
F333L |
probably damaging |
Het |
| Tnpo3 |
T |
C |
6: 29,589,213 (GRCm39) |
I46V |
probably benign |
Het |
| Traf5 |
G |
T |
1: 191,746,984 (GRCm39) |
N112K |
probably damaging |
Het |
| Trim12c |
A |
T |
7: 103,991,165 (GRCm39) |
M263K |
unknown |
Het |
| Trim55 |
A |
G |
3: 19,727,011 (GRCm39) |
I359M |
probably benign |
Het |
| Utp20 |
A |
T |
10: 88,594,306 (GRCm39) |
W2140R |
probably benign |
Het |
| Vgll3 |
T |
C |
16: 65,624,964 (GRCm39) |
V104A |
probably damaging |
Het |
| Vmn2r74 |
C |
A |
7: 85,605,227 (GRCm39) |
G474* |
probably null |
Het |
| Vps18 |
G |
A |
2: 119,123,237 (GRCm39) |
A98T |
probably benign |
Het |
| Zfand4 |
T |
A |
6: 116,291,169 (GRCm39) |
S387T |
probably damaging |
Het |
| Zfp808 |
A |
G |
13: 62,320,934 (GRCm39) |
Y721C |
probably damaging |
Het |
|
| Other mutations in Adgrg7 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01783:Adgrg7
|
APN |
16 |
56,568,282 (GRCm39) |
critical splice donor site |
probably null |
|
|
IGL03122:Adgrg7
|
APN |
16 |
56,590,725 (GRCm39) |
splice site |
probably benign |
|
|
orchard
|
UTSW |
16 |
56,545,342 (GRCm39) |
missense |
probably damaging |
1.00 |
|
sevin
|
UTSW |
16 |
56,562,769 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0632:Adgrg7
|
UTSW |
16 |
56,562,952 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
R0673:Adgrg7
|
UTSW |
16 |
56,593,849 (GRCm39) |
missense |
possibly damaging |
0.48 |
|
R1690:Adgrg7
|
UTSW |
16 |
56,615,993 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R2009:Adgrg7
|
UTSW |
16 |
56,582,236 (GRCm39) |
missense |
probably benign |
0.08 |
|
R2017:Adgrg7
|
UTSW |
16 |
56,553,169 (GRCm39) |
missense |
probably benign |
0.02 |
|
R2132:Adgrg7
|
UTSW |
16 |
56,588,281 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2153:Adgrg7
|
UTSW |
16 |
56,572,791 (GRCm39) |
missense |
possibly damaging |
0.75 |
|
R2229:Adgrg7
|
UTSW |
16 |
56,572,766 (GRCm39) |
missense |
probably benign |
|
|
R2436:Adgrg7
|
UTSW |
16 |
56,582,308 (GRCm39) |
missense |
possibly damaging |
0.78 |
|
R2878:Adgrg7
|
UTSW |
16 |
56,570,817 (GRCm39) |
missense |
probably benign |
0.14 |
|
R2981:Adgrg7
|
UTSW |
16 |
56,570,769 (GRCm39) |
critical splice donor site |
probably null |
|
|
R4014:Adgrg7
|
UTSW |
16 |
56,562,651 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4023:Adgrg7
|
UTSW |
16 |
56,550,661 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4024:Adgrg7
|
UTSW |
16 |
56,550,661 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4026:Adgrg7
|
UTSW |
16 |
56,550,661 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4551:Adgrg7
|
UTSW |
16 |
56,568,375 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4834:Adgrg7
|
UTSW |
16 |
56,553,232 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5041:Adgrg7
|
UTSW |
16 |
56,550,711 (GRCm39) |
missense |
probably benign |
0.21 |
|
R5145:Adgrg7
|
UTSW |
16 |
56,562,682 (GRCm39) |
missense |
probably benign |
0.04 |
|
R5377:Adgrg7
|
UTSW |
16 |
56,550,669 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R5549:Adgrg7
|
UTSW |
16 |
56,570,790 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5915:Adgrg7
|
UTSW |
16 |
56,550,748 (GRCm39) |
splice site |
probably null |
|
|
R5957:Adgrg7
|
UTSW |
16 |
56,593,790 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R6146:Adgrg7
|
UTSW |
16 |
56,593,829 (GRCm39) |
missense |
probably benign |
0.21 |
|
R6198:Adgrg7
|
UTSW |
16 |
56,597,556 (GRCm39) |
missense |
possibly damaging |
0.64 |
|
R6233:Adgrg7
|
UTSW |
16 |
56,599,005 (GRCm39) |
missense |
possibly damaging |
0.52 |
|
R6337:Adgrg7
|
UTSW |
16 |
56,572,788 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R6633:Adgrg7
|
UTSW |
16 |
56,550,649 (GRCm39) |
missense |
probably benign |
0.05 |
|
R6693:Adgrg7
|
UTSW |
16 |
56,590,587 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R6812:Adgrg7
|
UTSW |
16 |
56,616,161 (GRCm39) |
start gained |
probably benign |
|
|
R6841:Adgrg7
|
UTSW |
16 |
56,570,787 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6868:Adgrg7
|
UTSW |
16 |
56,593,839 (GRCm39) |
missense |
probably benign |
|
|
R7076:Adgrg7
|
UTSW |
16 |
56,562,769 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7146:Adgrg7
|
UTSW |
16 |
56,550,605 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7232:Adgrg7
|
UTSW |
16 |
56,597,515 (GRCm39) |
splice site |
probably null |
|
|
R7266:Adgrg7
|
UTSW |
16 |
56,590,674 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7376:Adgrg7
|
UTSW |
16 |
56,545,342 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7390:Adgrg7
|
UTSW |
16 |
56,553,207 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R7401:Adgrg7
|
UTSW |
16 |
56,562,781 (GRCm39) |
missense |
probably benign |
0.43 |
|
R7496:Adgrg7
|
UTSW |
16 |
56,553,220 (GRCm39) |
missense |
probably benign |
|
|
R7540:Adgrg7
|
UTSW |
16 |
56,570,792 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8354:Adgrg7
|
UTSW |
16 |
56,616,045 (GRCm39) |
start gained |
probably benign |
|
|
R8372:Adgrg7
|
UTSW |
16 |
56,616,114 (GRCm39) |
start gained |
probably benign |
|
|
R8393:Adgrg7
|
UTSW |
16 |
56,582,477 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8454:Adgrg7
|
UTSW |
16 |
56,616,045 (GRCm39) |
start gained |
probably benign |
|
|
R8723:Adgrg7
|
UTSW |
16 |
56,582,282 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8891:Adgrg7
|
UTSW |
16 |
56,572,762 (GRCm39) |
missense |
probably benign |
0.03 |
|
R9017:Adgrg7
|
UTSW |
16 |
56,553,211 (GRCm39) |
missense |
probably benign |
0.01 |
|
R9570:Adgrg7
|
UTSW |
16 |
56,570,813 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9604:Adgrg7
|
UTSW |
16 |
56,597,570 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R9628:Adgrg7
|
UTSW |
16 |
56,553,193 (GRCm39) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- AGAGGCTTCATGGTTCTTATCAG -3'
(R):5'- CTCAGTTGATGATGCTTTGCAG -3'
Sequencing Primer
(F):5'- GCTTCATGGTTCTTATCAGAAGGAAG -3'
(R):5'- ATGCTTTGCAGTTTCTGTGATTTTAC -3'
|
| Posted On |
2020-06-30 |
Incidental Mutation