Mutagenetix > Incidental Mutation

Incidental Mutation 'R8158:Agrn'

633351

Institutional Source

Beutler Lab

Gene Symbol

Agrn

Ensembl Gene

ENSMUSG00000041936

Gene Name

agrin

Synonyms

Agrin, NMF380, nmf380

MMRRC Submission

067584-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.430) question?

Stock #

R8158 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

156249747-156281945 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 156258346 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 1040 (V1040A)

Ref Sequence

ENSEMBL: ENSMUSP00000137931 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071248] [ENSMUST00000105574] [ENSMUST00000105575] [ENSMUST00000180572]

AlphaFold

A2ASQ1

Predicted Effect

probably benign
Transcript: ENSMUST00000071248
AA Change: V933A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000071229
Gene: ENSMUSG00000041936
AA Change: V933A

Domain	Start	End	E-Value	Type
transmembrane domain	25	47	N/A	INTRINSIC
FOLN	66	91	8.25e-6	SMART
KAZAL	91	137	1.22e-17	SMART
FOLN	142	166	7.58e-5	SMART
EGF_like	142	181	7.38e1	SMART
KAZAL	166	212	1.51e-13	SMART
KAZAL	241	284	1.8e-6	SMART
KAZAL	310	356	1.55e-10	SMART
FOLN	362	384	8.25e-6	SMART
KAZAL	384	429	1.14e-17	SMART
KAZAL	449	494	6.43e-17	SMART
FOLN	496	519	2.94e-2	SMART
KAZAL	507	559	8.96e-16	SMART
low complexity region	565	572	N/A	INTRINSIC
KAZAL	599	645	1.12e-16	SMART
EGF_Lam	688	739	3.29e-15	SMART
EGF_Lam	742	786	6.7e-7	SMART
FOLN	795	817	1.94e-2	SMART
KAZAL	817	864	3.9e-16	SMART
low complexity region	889	906	N/A	INTRINSIC
low complexity region	949	978	N/A	INTRINSIC
SEA	1014	1139	5.57e-35	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000105574
AA Change: V933A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000101199
Gene: ENSMUSG00000041936
AA Change: V933A

Domain	Start	End	E-Value	Type
transmembrane domain	25	47	N/A	INTRINSIC
FOLN	66	91	8.25e-6	SMART
KAZAL	91	137	1.22e-17	SMART
FOLN	142	166	7.58e-5	SMART
EGF_like	142	181	7.38e1	SMART
KAZAL	166	212	1.51e-13	SMART
KAZAL	241	284	1.8e-6	SMART
KAZAL	310	356	1.55e-10	SMART
FOLN	362	384	8.25e-6	SMART
KAZAL	384	429	1.14e-17	SMART
KAZAL	449	494	6.43e-17	SMART
FOLN	496	519	2.94e-2	SMART
KAZAL	507	559	8.96e-16	SMART
low complexity region	565	572	N/A	INTRINSIC
KAZAL	599	645	1.12e-16	SMART
EGF_Lam	688	739	3.29e-15	SMART
EGF_Lam	742	786	6.7e-7	SMART
FOLN	795	817	1.94e-2	SMART
KAZAL	817	864	3.9e-16	SMART
low complexity region	889	906	N/A	INTRINSIC
low complexity region	949	978	N/A	INTRINSIC
SEA	1014	1136	2.26e-35	SMART
low complexity region	1142	1169	N/A	INTRINSIC
low complexity region	1183	1198	N/A	INTRINSIC
EGF	1214	1249	1.49e-4	SMART
LamG	1274	1410	4e-45	SMART
EGF	1434	1468	2.23e-3	SMART
EGF	1473	1507	7.13e-2	SMART
LamG	1542	1678	6.51e-36	SMART
EGF	1699	1735	4.35e-6	SMART
LamG	1771	1907	5.01e-37	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000105575
AA Change: V933A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000101200
Gene: ENSMUSG00000041936
AA Change: V933A

Domain	Start	End	E-Value	Type
transmembrane domain	25	47	N/A	INTRINSIC
FOLN	66	91	8.25e-6	SMART
KAZAL	91	137	1.22e-17	SMART
FOLN	142	166	7.58e-5	SMART
EGF_like	142	181	7.38e1	SMART
KAZAL	166	212	1.51e-13	SMART
KAZAL	241	284	1.8e-6	SMART
KAZAL	310	356	1.55e-10	SMART
FOLN	362	384	8.25e-6	SMART
KAZAL	384	429	1.14e-17	SMART
KAZAL	449	494	6.43e-17	SMART
FOLN	496	519	2.94e-2	SMART
KAZAL	507	559	8.96e-16	SMART
low complexity region	565	572	N/A	INTRINSIC
KAZAL	599	645	1.12e-16	SMART
EGF_Lam	688	739	3.29e-15	SMART
EGF_Lam	742	786	6.7e-7	SMART
FOLN	795	817	1.94e-2	SMART
KAZAL	817	864	3.9e-16	SMART
low complexity region	889	906	N/A	INTRINSIC
low complexity region	949	978	N/A	INTRINSIC
SEA	1014	1136	2.26e-35	SMART
low complexity region	1142	1169	N/A	INTRINSIC
low complexity region	1183	1198	N/A	INTRINSIC
EGF	1214	1249	1.49e-4	SMART
LamG	1274	1410	4e-45	SMART
EGF	1434	1468	2.23e-3	SMART
EGF	1473	1507	7.13e-2	SMART
LamG	1542	1682	9.2e-36	SMART
EGF	1703	1739	4.35e-6	SMART
LamG	1794	1930	5.01e-37	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000180572
AA Change: V1040A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000137931
Gene: ENSMUSG00000041936
AA Change: V1040A

Domain	Start	End	E-Value	Type
signal peptide	1	31	N/A	INTRINSIC
Pfam:NtA	32	159	5.1e-91	PFAM
FOLN	173	198	8.25e-6	SMART
KAZAL	198	244	1.22e-17	SMART
FOLN	249	273	7.58e-5	SMART
EGF_like	249	288	7.38e1	SMART
KAZAL	273	319	1.51e-13	SMART
KAZAL	348	391	1.8e-6	SMART
KAZAL	417	463	1.55e-10	SMART
FOLN	469	491	8.25e-6	SMART
KAZAL	491	536	1.14e-17	SMART
KAZAL	556	601	6.43e-17	SMART
FOLN	603	626	2.94e-2	SMART
KAZAL	614	666	8.96e-16	SMART
low complexity region	672	679	N/A	INTRINSIC
KAZAL	706	752	1.12e-16	SMART
EGF_Lam	795	846	3.29e-15	SMART
EGF_Lam	849	893	6.7e-7	SMART
FOLN	902	924	1.94e-2	SMART
KAZAL	924	971	3.9e-16	SMART
low complexity region	996	1013	N/A	INTRINSIC
low complexity region	1056	1085	N/A	INTRINSIC
SEA	1121	1243	2.26e-35	SMART
low complexity region	1249	1276	N/A	INTRINSIC
low complexity region	1290	1305	N/A	INTRINSIC
EGF	1321	1356	1.49e-4	SMART
LamG	1381	1517	4e-45	SMART
EGF	1541	1575	2.23e-3	SMART
EGF	1580	1614	7.13e-2	SMART
LamG	1649	1785	6.51e-36	SMART
EGF	1806	1842	4.35e-6	SMART
LamG	1878	2014	5.01e-37	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.3%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in this gene was found. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]
PHENOTYPE: Nullizygous mice display embryonic failure of NMJ formation, inability to breathe or move and perinatal lethality. Homozygotes for an ENU-induced allele show poor hindlimb motor control, myopathy, muscle atrophy, spasms and fiber-type switching, NMJ disaggregation, camptodactyly and premature death. [provided by MGI curators]

Allele List at MGI

All alleles(12) : Targeted, knock-out(4) Targeted, other(1) Gene trapped(7)

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abl2	T	A	1: 156,469,639 (GRCm39)	S968T	probably benign	Het
Adcy8	T	C	15: 64,655,655 (GRCm39)	D608G	probably benign	Het
Adgb	C	T	10: 10,254,478 (GRCm39)	V1162M	probably benign	Het
Amer3	A	T	1: 34,626,741 (GRCm39)	T327S	possibly damaging	Het
Apaf1	C	T	10: 90,895,520 (GRCm39)	C426Y	probably benign	Het
Bsn	T	C	9: 107,987,232 (GRCm39)	D2840G	unknown	Het
Camsap1	A	T	2: 25,834,440 (GRCm39)	C406*	probably null	Het
Cbr2	A	T	11: 120,621,123 (GRCm39)	M158K	probably damaging	Het
Cgrrf1	T	C	14: 47,091,192 (GRCm39)	S239P	probably benign	Het
Clnk	C	A	5: 38,952,254 (GRCm39)		probably null	Het
Csad	T	C	15: 102,086,197 (GRCm39)	M445V	probably damaging	Het
Dnajb7	T	A	15: 81,291,600 (GRCm39)	N246Y	probably damaging	Het
Dok6	T	C	18: 89,492,071 (GRCm39)	I169V	probably benign	Het
H2bc14	T	C	13: 21,906,631 (GRCm39)	Y122H	probably benign	Het
Il1f10	T	G	2: 24,181,267 (GRCm39)	I11R	possibly damaging	Het
Irs1	C	T	1: 82,267,254 (GRCm39)	V321M	probably damaging	Het
Itga9	T	C	9: 118,706,211 (GRCm39)	F938L	probably damaging	Het
Kif7	T	C	7: 79,354,442 (GRCm39)	D781G	probably damaging	Het
Klhl41	A	T	2: 69,501,505 (GRCm39)	N322I	probably damaging	Het
Krtap31-1	T	G	11: 99,798,901 (GRCm39)	C35G	possibly damaging	Het
Lsm11	A	T	11: 45,824,824 (GRCm39)	D234E	probably benign	Het
Med6	T	C	12: 81,620,677 (GRCm39)	K223R	probably benign	Het
Mfsd4b2	G	A	10: 39,798,064 (GRCm39)	T97I	probably benign	Het
Mrgpra1	A	T	7: 46,985,204 (GRCm39)	C158*	probably null	Het
Nr2e1	T	C	10: 42,458,881 (GRCm39)	T8A	probably benign	Het
Oas2	T	C	5: 120,887,838 (GRCm39)	M1V	probably null	Het
Or13a27	T	C	7: 139,925,162 (GRCm39)	I247V	probably benign	Het
Or14a260	T	A	7: 85,984,885 (GRCm39)	I240F	probably benign	Het
Or1e33	T	A	11: 73,738,697 (GRCm39)	M85L	probably benign	Het
Or8k17	T	C	2: 86,066,504 (GRCm39)	Y218C	probably damaging	Het
Or8w1	T	A	2: 87,465,233 (GRCm39)	N286I	probably damaging	Het
Otof	C	T	5: 30,537,538 (GRCm39)	G1257D	probably benign	Het
Phf2	T	C	13: 48,971,236 (GRCm39)	T479A	probably benign	Het
Pik3c2a	T	C	7: 115,942,232 (GRCm39)	N1516S	probably benign	Het
Qrich1	C	A	9: 108,433,236 (GRCm39)	T622K	probably damaging	Het
Rasip1	A	G	7: 45,281,943 (GRCm39)	K482R	probably damaging	Het
Rgl2	T	C	17: 34,155,918 (GRCm39)	F698L	probably benign	Het
Scarb1	T	C	5: 125,380,201 (GRCm39)	D124G	probably benign	Het
Sclt1	A	G	3: 41,625,917 (GRCm39)	I350T	probably benign	Het
Sec23ip	T	G	7: 128,369,364 (GRCm39)	V696G	probably damaging	Het
Serpina1e	G	T	12: 103,917,354 (GRCm39)	T105K	probably benign	Het
Sin3a	A	G	9: 57,020,828 (GRCm39)		probably null	Het
Slc34a2	A	G	5: 53,218,182 (GRCm39)	T154A	probably damaging	Het
Slc9a3	T	C	13: 74,303,241 (GRCm39)	L178P	probably damaging	Het
Smarca2	A	C	19: 26,659,448 (GRCm39)	I913L	probably benign	Het
Spaca7b	T	A	8: 11,715,056 (GRCm39)	D46V	probably damaging	Het
Tcea3	G	A	4: 136,001,027 (GRCm39)		probably null	Het
Tektl1	G	C	10: 78,584,509 (GRCm39)	Q338E	probably benign	Het
Tmcc1	T	A	6: 116,020,435 (GRCm39)	H339L		Het
Trpm2	G	T	10: 77,783,731 (GRCm39)	H247Q	probably damaging	Het
Tyr	T	A	7: 87,121,724 (GRCm39)	D356V	probably damaging	Het
Vmn2r54	A	T	7: 12,349,888 (GRCm39)	C565S	probably damaging	Het
Vmn2r82	A	C	10: 79,213,636 (GRCm39)	N74T	probably benign	Het
Zcchc4	T	C	5: 52,973,260 (GRCm39)	Y375H	probably damaging	Het
Zfhx3	T	A	8: 109,675,353 (GRCm39)	D2134E	possibly damaging	Het
Zfhx4	T	A	3: 5,464,010 (GRCm39)	Y1414*	probably null	Het
Zfp282	A	T	6: 47,867,626 (GRCm39)	E267D	possibly damaging	Het
Zfp442	A	T	2: 150,251,096 (GRCm39)	C269S	possibly damaging	Het
Zp3	T	A	5: 136,014,418 (GRCm39)	S246T	probably benign	Het

Other mutations in Agrn

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00479:Agrn	APN	4	156,255,029 (GRCm39)	splice site	probably benign
IGL00811:Agrn	APN	4	156,253,231 (GRCm39)	missense	possibly damaging	0.70
IGL01066:Agrn	APN	4	156,261,800 (GRCm39)	missense	probably benign	0.00
IGL01412:Agrn	APN	4	156,255,491 (GRCm39)	splice site	probably benign
IGL01414:Agrn	APN	4	156,279,696 (GRCm39)	splice site	probably null
IGL02075:Agrn	APN	4	156,254,667 (GRCm39)	missense	probably benign	0.40
IGL02609:Agrn	APN	4	156,259,680 (GRCm39)	splice site	probably benign
IGL02669:Agrn	APN	4	156,259,018 (GRCm39)	splice site	probably benign
IGL02671:Agrn	APN	4	156,259,018 (GRCm39)	splice site	probably benign
IGL02672:Agrn	APN	4	156,259,018 (GRCm39)	splice site	probably benign
IGL02674:Agrn	APN	4	156,259,018 (GRCm39)	splice site	probably benign
IGL02724:Agrn	APN	4	156,257,264 (GRCm39)	nonsense	probably null
IGL02804:Agrn	APN	4	156,258,512 (GRCm39)	missense	probably benign	0.00
IGL02986:Agrn	APN	4	156,263,311 (GRCm39)	missense	possibly damaging	0.84
IGL03160:Agrn	APN	4	156,254,820 (GRCm39)	missense	probably damaging	0.98
BB004:Agrn	UTSW	4	156,257,266 (GRCm39)	missense	probably damaging	0.99
BB014:Agrn	UTSW	4	156,257,266 (GRCm39)	missense	probably damaging	0.99
F6893:Agrn	UTSW	4	156,258,636 (GRCm39)	missense	probably benign
R0092:Agrn	UTSW	4	156,263,410 (GRCm39)	missense	probably damaging	1.00
R0100:Agrn	UTSW	4	156,259,415 (GRCm39)	missense	probably damaging	1.00
R0100:Agrn	UTSW	4	156,259,415 (GRCm39)	missense	probably damaging	1.00
R0482:Agrn	UTSW	4	156,258,012 (GRCm39)	missense	probably damaging	0.98
R0531:Agrn	UTSW	4	156,263,891 (GRCm39)	missense	probably benign	0.38
R0536:Agrn	UTSW	4	156,264,010 (GRCm39)	missense	probably benign	0.01
R0690:Agrn	UTSW	4	156,258,910 (GRCm39)	missense	probably damaging	1.00
R0750:Agrn	UTSW	4	156,251,394 (GRCm39)	nonsense	probably null
R1079:Agrn	UTSW	4	156,261,682 (GRCm39)	missense	probably damaging	1.00
R1199:Agrn	UTSW	4	156,256,756 (GRCm39)	missense	probably benign	0.00
R1222:Agrn	UTSW	4	156,261,842 (GRCm39)	missense	probably damaging	0.99
R1534:Agrn	UTSW	4	156,261,141 (GRCm39)	missense	probably damaging	1.00
R1587:Agrn	UTSW	4	156,263,897 (GRCm39)	missense	probably damaging	0.99
R1625:Agrn	UTSW	4	156,257,317 (GRCm39)	missense	probably damaging	1.00
R1698:Agrn	UTSW	4	156,251,015 (GRCm39)	missense	probably benign	0.03
R1717:Agrn	UTSW	4	156,250,976 (GRCm39)	frame shift	probably null
R1718:Agrn	UTSW	4	156,250,976 (GRCm39)	frame shift	probably null
R1721:Agrn	UTSW	4	156,259,630 (GRCm39)	nonsense	probably null
R1765:Agrn	UTSW	4	156,261,284 (GRCm39)	nonsense	probably null
R1840:Agrn	UTSW	4	156,251,872 (GRCm39)	missense	probably damaging	1.00
R1865:Agrn	UTSW	4	156,250,976 (GRCm39)	frame shift	probably null
R2105:Agrn	UTSW	4	156,261,756 (GRCm39)	nonsense	probably null
R2265:Agrn	UTSW	4	156,263,675 (GRCm39)	missense	probably damaging	0.99
R2266:Agrn	UTSW	4	156,263,675 (GRCm39)	missense	probably damaging	0.99
R2269:Agrn	UTSW	4	156,263,675 (GRCm39)	missense	probably damaging	0.99
R2382:Agrn	UTSW	4	156,260,973 (GRCm39)	missense	probably damaging	0.97
R2497:Agrn	UTSW	4	156,258,268 (GRCm39)	missense	probably benign	0.28
R2509:Agrn	UTSW	4	156,250,881 (GRCm39)	splice site	probably null
R2510:Agrn	UTSW	4	156,250,881 (GRCm39)	splice site	probably null
R2511:Agrn	UTSW	4	156,250,881 (GRCm39)	splice site	probably null
R2994:Agrn	UTSW	4	156,251,785 (GRCm39)	missense	possibly damaging	0.79
R3824:Agrn	UTSW	4	156,253,759 (GRCm39)	missense	probably damaging	1.00
R4736:Agrn	UTSW	4	156,256,858 (GRCm39)	missense	probably benign	0.38
R4755:Agrn	UTSW	4	156,257,979 (GRCm39)	intron	probably benign
R4853:Agrn	UTSW	4	156,270,007 (GRCm39)	critical splice donor site	probably null
R4878:Agrn	UTSW	4	156,255,302 (GRCm39)	missense	probably damaging	1.00
R5117:Agrn	UTSW	4	156,270,010 (GRCm39)	missense	probably benign	0.30
R5228:Agrn	UTSW	4	156,251,403 (GRCm39)	missense	probably damaging	1.00
R5236:Agrn	UTSW	4	156,263,315 (GRCm39)	missense	possibly damaging	0.93
R5269:Agrn	UTSW	4	156,253,447 (GRCm39)	missense	probably benign	0.10
R5282:Agrn	UTSW	4	156,257,492 (GRCm39)	missense	probably damaging	1.00
R5449:Agrn	UTSW	4	156,251,737 (GRCm39)	critical splice donor site	probably null
R5560:Agrn	UTSW	4	156,262,954 (GRCm39)	missense	probably damaging	0.99
R5668:Agrn	UTSW	4	156,251,770 (GRCm39)	missense	probably damaging	0.97
R5725:Agrn	UTSW	4	156,258,332 (GRCm39)	missense	probably benign	0.25
R5967:Agrn	UTSW	4	156,259,560 (GRCm39)	missense	probably damaging	1.00
R6226:Agrn	UTSW	4	156,258,066 (GRCm39)	missense	probably damaging	0.96
R6338:Agrn	UTSW	4	156,255,042 (GRCm39)	missense	probably benign	0.17
R6351:Agrn	UTSW	4	156,263,891 (GRCm39)	missense	probably benign	0.00
R6437:Agrn	UTSW	4	156,261,235 (GRCm39)	missense	probably damaging	0.96
R6490:Agrn	UTSW	4	156,251,819 (GRCm39)	nonsense	probably null
R6909:Agrn	UTSW	4	156,261,464 (GRCm39)	missense	possibly damaging	0.90
R7110:Agrn	UTSW	4	156,263,332 (GRCm39)	missense	possibly damaging	0.88
R7123:Agrn	UTSW	4	156,257,297 (GRCm39)	missense	probably benign
R7163:Agrn	UTSW	4	156,262,966 (GRCm39)	missense	probably damaging	1.00
R7180:Agrn	UTSW	4	156,256,296 (GRCm39)	missense	probably benign	0.00
R7251:Agrn	UTSW	4	156,259,063 (GRCm39)	missense	probably damaging	1.00
R7289:Agrn	UTSW	4	156,263,389 (GRCm39)	missense	probably damaging	1.00
R7335:Agrn	UTSW	4	156,260,989 (GRCm39)	missense	probably damaging	1.00
R7336:Agrn	UTSW	4	156,259,371 (GRCm39)	nonsense	probably null
R7406:Agrn	UTSW	4	156,256,758 (GRCm39)	missense	possibly damaging	0.93
R7460:Agrn	UTSW	4	156,258,881 (GRCm39)	missense	probably damaging	0.98
R7531:Agrn	UTSW	4	156,254,261 (GRCm39)	missense	probably damaging	1.00
R7585:Agrn	UTSW	4	156,255,131 (GRCm39)	missense	probably benign	0.08
R7646:Agrn	UTSW	4	156,279,811 (GRCm39)	missense	probably damaging	0.99
R7652:Agrn	UTSW	4	156,253,675 (GRCm39)	critical splice donor site	probably null
R7714:Agrn	UTSW	4	156,279,854 (GRCm39)	missense	probably damaging	1.00
R7751:Agrn	UTSW	4	156,260,886 (GRCm39)	missense	probably damaging	1.00
R7852:Agrn	UTSW	4	156,253,514 (GRCm39)	missense	probably benign	0.01
R7927:Agrn	UTSW	4	156,257,266 (GRCm39)	missense	probably damaging	0.99
R8039:Agrn	UTSW	4	156,253,468 (GRCm39)	missense	probably benign	0.12
R8056:Agrn	UTSW	4	156,254,868 (GRCm39)	missense	probably benign
R8061:Agrn	UTSW	4	156,263,411 (GRCm39)	missense	probably damaging	1.00
R8159:Agrn	UTSW	4	156,256,825 (GRCm39)	missense	probably benign	0.27
R8325:Agrn	UTSW	4	156,258,119 (GRCm39)	missense	probably benign	0.01
R8338:Agrn	UTSW	4	156,253,018 (GRCm39)	missense	probably benign	0.01
R8739:Agrn	UTSW	4	156,257,045 (GRCm39)	missense	probably benign
R8956:Agrn	UTSW	4	156,250,995 (GRCm39)	missense	probably damaging	0.99
R9094:Agrn	UTSW	4	156,253,264 (GRCm39)	missense	probably benign	0.01
R9112:Agrn	UTSW	4	156,261,514 (GRCm39)	missense	probably damaging	1.00
R9384:Agrn	UTSW	4	156,257,106 (GRCm39)	missense	probably damaging	1.00
R9472:Agrn	UTSW	4	156,254,841 (GRCm39)	missense
R9619:Agrn	UTSW	4	156,258,490 (GRCm39)	missense	probably benign	0.00
R9629:Agrn	UTSW	4	156,257,094 (GRCm39)	nonsense	probably null
R9732:Agrn	UTSW	4	156,258,446 (GRCm39)	missense	probably benign	0.13
R9749:Agrn	UTSW	4	156,258,114 (GRCm39)	missense	probably benign	0.02
R9757:Agrn	UTSW	4	156,261,235 (GRCm39)	missense	probably benign	0.03
R9792:Agrn	UTSW	4	156,261,129 (GRCm39)	missense	probably benign	0.09
R9793:Agrn	UTSW	4	156,261,129 (GRCm39)	missense	probably benign	0.09
Z1177:Agrn	UTSW	4	156,264,033 (GRCm39)	missense	possibly damaging	0.95
Z1177:Agrn	UTSW	4	156,256,001 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GAAACGCAGTCTAAGCCCAG -3'
(R):5'- TGACTCGAGCCCCTAACATC -3'

Sequencing Primer
(F):5'- AGTCTAAGCCCAGCCCCG -3'
(R):5'- ACATCCGTCTTTTTGCAGAGAG -3'

Posted On

2020-06-30