Mutagenetix > Incidental Mutation

Incidental Mutation 'R8158:Vmn2r82'

633381

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r82

Ensembl Gene

ENSMUSG00000091468

Gene Name

vomeronasal 2, receptor 82

Synonyms

EG624845

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.076) question?

Stock #

R8158 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

79356576-79397198 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 79377802 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Threonine at position 74 (N74T)

Ref Sequence

ENSEMBL: ENSMUSP00000130114 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170596]

AlphaFold

G3UWA2

Predicted Effect

probably benign
Transcript: ENSMUST00000170596
AA Change: N74T

PolyPhen 2 Score 0.119 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000130114
Gene: ENSMUSG00000091468
AA Change: N74T

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	79	474	6e-35	PFAM
Pfam:NCD3G	517	570	9.3e-22	PFAM
Pfam:7tm_3	603	838	6.5e-49	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700016D06Rik	T	A	8: 11,665,056	D46V	probably damaging	Het
Abl2	T	A	1: 156,642,069	S968T	probably benign	Het
Adcy8	T	C	15: 64,783,806	D608G	probably benign	Het
Adgb	C	T	10: 10,378,734	V1162M	probably benign	Het
Agrn	A	G	4: 156,173,889	V1040A	probably benign	Het
Amer3	A	T	1: 34,587,660	T327S	possibly damaging	Het
Apaf1	C	T	10: 91,059,658	C426Y	probably benign	Het
Bsn	T	C	9: 108,110,033	D2840G	unknown	Het
Camsap1	A	T	2: 25,944,428	C406*	probably null	Het
Cbr2	A	T	11: 120,730,297	M158K	probably damaging	Het
Ccdc105	G	C	10: 78,748,675	Q338E	probably benign	Het
Cgrrf1	T	C	14: 46,853,735	S239P	probably benign	Het
Clnk	C	A	5: 38,794,911		probably null	Het
Csad	T	C	15: 102,177,762	M445V	probably damaging	Het
Dnajb7	T	A	15: 81,407,399	N246Y	probably damaging	Het
Dok6	T	C	18: 89,473,947	I169V	probably benign	Het
Hist1h2bm	T	C	13: 21,722,461	Y122H	probably benign	Het
Il1f10	T	G	2: 24,291,255	I11R	possibly damaging	Het
Irs1	C	T	1: 82,289,533	V321M	probably damaging	Het
Itga9	T	C	9: 118,877,143	F938L	probably damaging	Het
Kif7	T	C	7: 79,704,694	D781G	probably damaging	Het
Klhl41	A	T	2: 69,671,161	N322I	probably damaging	Het
Krtap31-1	T	G	11: 99,908,075	C35G	possibly damaging	Het
Lsm11	A	T	11: 45,933,997	D234E	probably benign	Het
Med6	T	C	12: 81,573,903	K223R	probably benign	Het
Mfsd4b2	G	A	10: 39,922,068	T97I	probably benign	Het
Mrgpra1	A	T	7: 47,335,456	C158*	probably null	Het
Nr2e1	T	C	10: 42,582,885	T8A	probably benign	Het
Oas2	T	C	5: 120,749,773	M1V	probably null	Het
Olfr1048	T	C	2: 86,236,160	Y218C	probably damaging	Het
Olfr1132	T	A	2: 87,634,889	N286I	probably damaging	Het
Olfr307	T	A	7: 86,335,677	I240F	probably benign	Het
Olfr393	T	A	11: 73,847,871	M85L	probably benign	Het
Olfr60	T	C	7: 140,345,249	I247V	probably benign	Het
Otof	C	T	5: 30,380,194	G1257D	probably benign	Het
Phf2	T	C	13: 48,817,760	T479A	probably benign	Het
Pik3c2a	T	C	7: 116,342,997	N1516S	probably benign	Het
Qrich1	C	A	9: 108,556,037	T622K	probably damaging	Het
Rasip1	A	G	7: 45,632,519	K482R	probably damaging	Het
Rgl2	T	C	17: 33,936,944	F698L	probably benign	Het
Scarb1	T	C	5: 125,303,137	D124G	probably benign	Het
Sclt1	A	G	3: 41,671,482	I350T	probably benign	Het
Sec23ip	T	G	7: 128,767,640	V696G	probably damaging	Het
Serpina1e	G	T	12: 103,951,095	T105K	probably benign	Het
Sin3a	A	G	9: 57,113,544		probably null	Het
Slc34a2	A	G	5: 53,060,840	T154A	probably damaging	Het
Slc9a3	T	C	13: 74,155,122	L178P	probably damaging	Het
Smarca2	A	C	19: 26,682,048	I913L	probably benign	Het
Tcea3	G	A	4: 136,273,716		probably null	Het
Tmcc1	T	A	6: 116,043,474	H339L		Het
Trpm2	G	T	10: 77,947,897	H247Q	probably damaging	Het
Tyr	T	A	7: 87,472,516	D356V	probably damaging	Het
Vmn2r54	A	T	7: 12,615,961	C565S	probably damaging	Het
Zcchc4	T	C	5: 52,815,918	Y375H	probably damaging	Het
Zfhx3	T	A	8: 108,948,721	D2134E	possibly damaging	Het
Zfhx4	T	A	3: 5,398,950	Y1414*	probably null	Het
Zfp282	A	T	6: 47,890,692	E267D	possibly damaging	Het
Zfp442	A	T	2: 150,409,176	C269S	possibly damaging	Het
Zp3	T	A	5: 135,985,564	S246T	probably benign	Het

Other mutations in Vmn2r82

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01800:Vmn2r82	APN	10	79356747	missense	probably benign	0.03
IGL01860:Vmn2r82	APN	10	79378857	missense	probably benign	0.18
IGL01927:Vmn2r82	APN	10	79378072	missense	probably damaging	1.00
IGL01929:Vmn2r82	APN	10	79378711	missense	probably damaging	1.00
IGL02028:Vmn2r82	APN	10	79379223	missense	probably benign
IGL02112:Vmn2r82	APN	10	79395999	missense	probably benign	0.19
IGL02632:Vmn2r82	APN	10	79356708	missense	probably benign	0.45
IGL02665:Vmn2r82	APN	10	79379371	missense	probably damaging	0.99
IGL02716:Vmn2r82	APN	10	79377844	missense	probably benign	0.20
IGL03030:Vmn2r82	APN	10	79381315	missense	possibly damaging	0.85
IGL03190:Vmn2r82	APN	10	79356809	splice site	probably null
IGL03349:Vmn2r82	APN	10	79377869	missense	probably benign	0.25
IGL03048:Vmn2r82	UTSW	10	79396626	missense	probably damaging	0.98
R0080:Vmn2r82	UTSW	10	79396505	missense	probably benign	0.00
R0193:Vmn2r82	UTSW	10	79381295	missense	probably damaging	1.00
R0217:Vmn2r82	UTSW	10	79378800	missense	possibly damaging	0.46
R0285:Vmn2r82	UTSW	10	79396557	missense	probably damaging	1.00
R1193:Vmn2r82	UTSW	10	79377905	nonsense	probably null
R1385:Vmn2r82	UTSW	10	79396491	nonsense	probably null
R1386:Vmn2r82	UTSW	10	79378711	missense	probably damaging	1.00
R1442:Vmn2r82	UTSW	10	79379367	missense	probably benign	0.03
R1467:Vmn2r82	UTSW	10	79396299	missense	probably benign	0.00
R1467:Vmn2r82	UTSW	10	79396299	missense	probably benign	0.00
R1518:Vmn2r82	UTSW	10	79378868	missense	probably damaging	1.00
R1538:Vmn2r82	UTSW	10	79356744	missense	possibly damaging	0.92
R1607:Vmn2r82	UTSW	10	79379419	missense	possibly damaging	0.67
R1812:Vmn2r82	UTSW	10	79379212	missense	probably benign	0.33
R1906:Vmn2r82	UTSW	10	79396510	missense	probably damaging	1.00
R1954:Vmn2r82	UTSW	10	79396056	missense	probably damaging	1.00
R1972:Vmn2r82	UTSW	10	79378846	missense	probably damaging	1.00
R2093:Vmn2r82	UTSW	10	79395979	missense	probably benign	0.30
R2156:Vmn2r82	UTSW	10	79378888	missense	probably damaging	1.00
R2202:Vmn2r82	UTSW	10	79356685	missense	probably benign
R2442:Vmn2r82	UTSW	10	79385376	missense	probably damaging	1.00
R2444:Vmn2r82	UTSW	10	79377868	missense	possibly damaging	0.65
R2857:Vmn2r82	UTSW	10	79381256	missense	probably damaging	0.98
R2858:Vmn2r82	UTSW	10	79381256	missense	probably damaging	0.98
R2884:Vmn2r82	UTSW	10	79396248	missense	probably benign	0.00
R2886:Vmn2r82	UTSW	10	79396248	missense	probably benign	0.00
R4369:Vmn2r82	UTSW	10	79396080	missense	probably benign	0.01
R4445:Vmn2r82	UTSW	10	79379040	missense	possibly damaging	0.87
R4589:Vmn2r82	UTSW	10	79356714	missense	probably damaging	1.00
R4703:Vmn2r82	UTSW	10	79378807	missense	probably damaging	1.00
R4908:Vmn2r82	UTSW	10	79378755	missense	probably benign	0.00
R4937:Vmn2r82	UTSW	10	79379176	missense	probably benign	0.01
R5199:Vmn2r82	UTSW	10	79396087	missense	probably damaging	1.00
R5391:Vmn2r82	UTSW	10	79356657	missense	probably null	0.01
R5601:Vmn2r82	UTSW	10	79396191	missense	probably damaging	1.00
R5635:Vmn2r82	UTSW	10	79378818	missense	probably benign	0.33
R6065:Vmn2r82	UTSW	10	79385376	missense	probably damaging	1.00
R6074:Vmn2r82	UTSW	10	79396543	missense	probably damaging	1.00
R6340:Vmn2r82	UTSW	10	79395893	missense	probably benign	0.00
R6474:Vmn2r82	UTSW	10	79379037	missense	possibly damaging	0.55
R6995:Vmn2r82	UTSW	10	79396543	missense	probably damaging	1.00
R7111:Vmn2r82	UTSW	10	79378771	missense	probably benign	0.22
R7212:Vmn2r82	UTSW	10	79379434	missense	probably benign	0.00
R7335:Vmn2r82	UTSW	10	79378888	missense	probably damaging	1.00
R7353:Vmn2r82	UTSW	10	79396618	missense	probably benign	0.11
R7354:Vmn2r82	UTSW	10	79356630	missense	probably benign	0.00
R7362:Vmn2r82	UTSW	10	79396617	missense	probably benign	0.00
R7378:Vmn2r82	UTSW	10	79396442	nonsense	probably null
R7430:Vmn2r82	UTSW	10	79381253	missense	probably damaging	1.00
R7509:Vmn2r82	UTSW	10	79396008	missense	possibly damaging	0.82
R7874:Vmn2r82	UTSW	10	79396511	missense	probably damaging	1.00
R7943:Vmn2r82	UTSW	10	79396245	missense	possibly damaging	0.74
R8324:Vmn2r82	UTSW	10	79378893	nonsense	probably null
R8340:Vmn2r82	UTSW	10	79381202	missense	probably benign	0.00
R8787:Vmn2r82	UTSW	10	79378060	missense	probably damaging	1.00
R8929:Vmn2r82	UTSW	10	79396707	missense	probably benign	0.00
R9018:Vmn2r82	UTSW	10	79396705	missense	probably damaging	1.00
R9399:Vmn2r82	UTSW	10	79378934	nonsense	probably null
R9517:Vmn2r82	UTSW	10	79377807	nonsense	probably null
R9587:Vmn2r82	UTSW	10	79379102	missense	possibly damaging	0.70
R9602:Vmn2r82	UTSW	10	79379046	missense	probably benign	0.07
Z1088:Vmn2r82	UTSW	10	79356622	missense	probably damaging	1.00
Z1177:Vmn2r82	UTSW	10	79356595	missense	probably benign	0.03
Z1177:Vmn2r82	UTSW	10	79396535	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGGTCTATAATTAACAAGGTCCCC -3'
(R):5'- GTAAGTGTAGCAGCTGATTTGC -3'

Sequencing Primer
(F):5'- AGGTCCCCCTTTCAGGATC -3'
(R):5'- TAATTAGGGATCACACTACTCAAACC -3'

Posted On

2020-06-30