Incidental Mutation 'R8163:Gpr25'
ID 633601
Institutional Source Beutler Lab
Gene Symbol Gpr25
Ensembl Gene ENSMUSG00000052759
Gene Name G protein-coupled receptor 25
Synonyms LOC383563
MMRRC Submission 067589-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R8163 (G1)
Quality Score 225.009
Status Validated
Chromosome 1
Chromosomal Location 136187535-136188611 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 136187596 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Glycine at position 339 (D339G)
Ref Sequence ENSEMBL: ENSMUSP00000083583 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000086395]
AlphaFold P0C5I1
Predicted Effect probably damaging
Transcript: ENSMUST00000086395
AA Change: D339G

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000083583
Gene: ENSMUSG00000052759
AA Change: D339G

Pfam:7tm_1 56 304 1.1e-40 PFAM
low complexity region 328 342 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.4%
  • 20x: 98.2%
Validation Efficiency 100% (53/53)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is intronless and encodes a member of the G-protein coupled receptor 1 family. G-protein coupled receptors are membrane proteins which activate signaling cascades as a response to extracellular stress. This gene has been linked to arterial stiffness. [provided by RefSeq, Nov 2012]
Allele List at MGI
Other mutations in this stock
Total: 51 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
9930022D16Rik A G 11: 109,304,883 (GRCm39) I11V unknown Het
Ago3 G T 4: 126,262,377 (GRCm39) T391K probably benign Het
Asap1 A G 15: 63,963,899 (GRCm39) W1100R probably damaging Het
Bbof1 T C 12: 84,473,536 (GRCm39) S289P possibly damaging Het
Cd3d T C 9: 44,896,952 (GRCm39) F109L probably benign Het
Cebpd A T 16: 15,705,841 (GRCm39) E218V probably damaging Het
Cfl1 T C 19: 5,543,528 (GRCm39) probably benign Het
Cpa2 A C 6: 30,564,350 (GRCm39) I394L probably damaging Het
Ddx50 C A 10: 62,475,678 (GRCm39) V348F possibly damaging Het
Dnajc28 G A 16: 91,413,795 (GRCm39) R150* probably null Het
Echs1 A G 7: 139,692,357 (GRCm39) V130A possibly damaging Het
Eif2s2 A G 2: 154,734,621 (GRCm39) S2P probably benign Het
Fastk A G 5: 24,649,273 (GRCm39) I38T probably benign Het
Fat3 G A 9: 15,871,055 (GRCm39) R3779C probably damaging Het
Fat4 A T 3: 39,033,881 (GRCm39) N2511I possibly damaging Het
Fbxw13 G A 9: 109,012,122 (GRCm39) T315I probably benign Het
Fzd5 A T 1: 64,774,352 (GRCm39) Y470N probably damaging Het
G530012D18Rik C G 1: 85,504,935 (GRCm39) D113E unknown Het
Igsf9b T A 9: 27,233,907 (GRCm39) probably null Het
Il5 T A 11: 53,614,813 (GRCm39) M125K possibly damaging Het
Iqub G A 6: 24,449,714 (GRCm39) T717I probably benign Het
Katnb1 T C 8: 95,823,014 (GRCm39) F403S probably damaging Het
Krt26 T C 11: 99,220,498 (GRCm39) I451V probably benign Het
Krt88 T C 15: 101,351,389 (GRCm39) L132P probably damaging Het
Kynu G T 2: 43,518,966 (GRCm39) G245V probably damaging Het
Lap3 C T 5: 45,669,389 (GRCm39) R513* probably null Het
Lin9 C T 1: 180,486,691 (GRCm39) R126W probably damaging Het
Lrrc37 A T 11: 103,506,688 (GRCm39) I1760K unknown Het
Lrrtm2 C T 18: 35,346,777 (GRCm39) R175H probably damaging Het
N4bp2l2 A G 5: 150,584,774 (GRCm39) L60P probably damaging Het
Ncr1 T C 7: 4,343,828 (GRCm39) F142S probably damaging Het
Nktr TAGAAG TAG 9: 121,579,929 (GRCm39) probably benign Het
Or5b95 A G 19: 12,657,552 (GRCm39) I27V probably benign Het
Parp16 T A 9: 65,137,231 (GRCm39) H152Q probably damaging Het
Plaa C G 4: 94,457,640 (GRCm39) V777L probably benign Het
Prnp A G 2: 131,778,908 (GRCm39) T187A probably benign Het
Pzp T G 6: 128,489,157 (GRCm39) I485L probably benign Het
Rc3h1 T A 1: 160,782,629 (GRCm39) Y703N probably damaging Het
Rngtt G A 4: 33,325,109 (GRCm39) C110Y probably damaging Het
Rps10 G A 17: 27,853,085 (GRCm39) R95C probably benign Het
Scn9a T A 2: 66,314,745 (GRCm39) I1658F probably damaging Het
Sec16a A T 2: 26,306,433 (GRCm39) W702R Het
Taf6 G T 5: 138,180,238 (GRCm39) Q339K possibly damaging Het
Tbcd C T 11: 121,384,711 (GRCm39) T315M probably benign Het
Tmem198 C T 1: 75,459,671 (GRCm39) P209S possibly damaging Het
Vmn2r78 G A 7: 86,603,660 (GRCm39) A613T probably damaging Het
Vps13c G A 9: 67,857,720 (GRCm39) E2651K probably benign Het
Wdfy4 C T 14: 32,873,545 (GRCm39) V255I Het
Zfhx3 A G 8: 109,675,925 (GRCm39) D2325G probably damaging Het
Zfp36l2 T C 17: 84,494,551 (GRCm39) N29D possibly damaging Het
Zfp692 T A 11: 58,201,199 (GRCm39) probably null Het
Other mutations in Gpr25
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL03028:Gpr25 APN 1 136,188,550 (GRCm39) missense probably benign 0.00
R1728:Gpr25 UTSW 1 136,188,448 (GRCm39) missense probably benign
R1729:Gpr25 UTSW 1 136,188,448 (GRCm39) missense probably benign
R1732:Gpr25 UTSW 1 136,187,866 (GRCm39) missense probably benign 0.01
R1739:Gpr25 UTSW 1 136,188,448 (GRCm39) missense probably benign
R1762:Gpr25 UTSW 1 136,188,448 (GRCm39) missense probably benign
R1783:Gpr25 UTSW 1 136,188,448 (GRCm39) missense probably benign
R1785:Gpr25 UTSW 1 136,188,448 (GRCm39) missense probably benign
R1907:Gpr25 UTSW 1 136,188,538 (GRCm39) missense probably benign 0.00
R2877:Gpr25 UTSW 1 136,188,553 (GRCm39) missense possibly damaging 0.86
R3081:Gpr25 UTSW 1 136,187,623 (GRCm39) missense possibly damaging 0.68
R5132:Gpr25 UTSW 1 136,188,103 (GRCm39) missense probably damaging 1.00
R7133:Gpr25 UTSW 1 136,188,559 (GRCm39) missense probably damaging 1.00
R8248:Gpr25 UTSW 1 136,188,415 (GRCm39) missense probably benign 0.03
R9051:Gpr25 UTSW 1 136,188,026 (GRCm39) missense probably benign 0.00
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2020-07-13