Mutagenetix > Incidental Mutation

Incidental Mutation 'R8168:Vmn1r174'

633903

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r174

Ensembl Gene

ENSMUSG00000090411

Gene Name

vomeronasal 1 receptor 174

Synonyms

V1rd22

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.053) question?

Stock #

R8168 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

23752270-23762403 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 23754671 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Valine at position 254 (D254V)

Ref Sequence

ENSEMBL: ENSMUSP00000126478 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167551]

AlphaFold

E9PYW5

Predicted Effect

probably damaging
Transcript: ENSMUST00000167551
AA Change: D254V

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000126478
Gene: ENSMUSG00000090411
AA Change: D254V

Domain	Start	End	E-Value	Type
Pfam:TAS2R	8	300	4e-9	PFAM
Pfam:V1R	43	300	5e-19	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

100% (44/44)

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810043G02Rik	G	A	10: 77,982,944	A150T	probably benign	Het
9430020K01Rik	A	T	18: 4,675,094	D952V	probably benign	Het
AI607873	A	C	1: 173,729,938	S411R	probably benign	Het
Ano4	A	G	10: 88,980,995	I652T	probably damaging	Het
Arhgef1	A	G	7: 24,925,406	T862A	probably benign	Het
Atp6v1b2	T	C	8: 69,108,331	S404P	possibly damaging	Het
Cfap54	A	T	10: 92,908,877	S2170T	unknown	Het
Copa	A	T	1: 172,099,672	H204L	probably damaging	Het
Cwc22	A	C	2: 77,927,271	V171G	probably damaging	Het
D230025D16Rik	C	T	8: 105,248,769	P330L	probably benign	Het
Dock5	A	T	14: 67,770,197		probably null	Het
Flrt1	G	A	19: 7,096,637	L182F	probably damaging	Het
Foxf1	T	C	8: 121,085,162	V255A	probably damaging	Het
Gnptab	G	T	10: 88,419,133	A194S	probably benign	Het
Gzf1	C	T	2: 148,684,766	R386C	probably damaging	Het
H2-Aa	T	A	17: 34,287,721	T16S	possibly damaging	Het
Hdgfrp2	T	C	17: 56,082,282	F52S	probably damaging	Het
Htt	A	G	5: 34,882,956	N2154S	probably benign	Het
Lama3	T	A	18: 12,506,942	W65R	probably null	Het
Mef2c	G	T	13: 83,656,350	L356F	probably damaging	Het
Mrgprb2	A	T	7: 48,552,019	S319R	probably benign	Het
Mtrr	C	T	13: 68,572,613	V288I	probably benign	Het
Myo18a	T	A	11: 77,821,142	I713N	probably damaging	Het
Nelfa	A	T	5: 33,921,907	N107K	possibly damaging	Het
Nim1k	A	T	13: 119,712,752	V202D	probably damaging	Het
Nlrc4	T	C	17: 74,445,211	T726A	probably benign	Het
Olfr1154	T	A	2: 87,903,199	H159L	probably damaging	Het
Olfr1208	T	A	2: 88,896,776	M274L	probably benign	Het
Prdm10	G	A	9: 31,346,967	A514T	probably benign	Het
Prg4	T	A	1: 150,455,850	E357D	unknown	Het
Ptp4a3	T	G	15: 73,756,846	Y152D	probably damaging	Het
Raph1	A	G	1: 60,499,620	C389R	unknown	Het
Rcc1	T	C	4: 132,335,785	E170G	probably benign	Het
Spag17	C	T	3: 100,034,984	T775M	possibly damaging	Het
Srcap	T	A	7: 127,542,523	V1825D	probably damaging	Het
Tex40	A	G	19: 6,922,652	S162P	possibly damaging	Het
Tshr	T	A	12: 91,511,965	H195Q	probably benign	Het
Vps13a	A	C	19: 16,749,548	I244M	probably benign	Het

Other mutations in Vmn1r174

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00331:Vmn1r174	APN	7	23754533	missense	possibly damaging	0.77
IGL00950:Vmn1r174	APN	7	23754486	missense	possibly damaging	0.96
IGL01484:Vmn1r174	APN	7	23754324	nonsense	probably null
IGL02014:Vmn1r174	APN	7	23754158	missense	probably damaging	1.00
IGL02190:Vmn1r174	APN	7	23754827	missense	unknown
IGL03265:Vmn1r174	APN	7	23754473	nonsense	probably null
IGL03335:Vmn1r174	APN	7	23754512	missense	probably benign	0.41
R0529:Vmn1r174	UTSW	7	23754197	missense	probably benign	0.00
R1489:Vmn1r174	UTSW	7	23754556	nonsense	probably null
R1645:Vmn1r174	UTSW	7	23754352	missense	possibly damaging	0.87
R1691:Vmn1r174	UTSW	7	23753912	start codon destroyed	probably null	1.00
R1753:Vmn1r174	UTSW	7	23754197	missense	probably benign	0.00
R1939:Vmn1r174	UTSW	7	23754107	missense	probably damaging	0.99
R1988:Vmn1r174	UTSW	7	23754625	missense	probably damaging	0.98
R2299:Vmn1r174	UTSW	7	23754004	missense	probably benign	0.08
R4429:Vmn1r174	UTSW	7	23754140	missense	probably benign	0.02
R4516:Vmn1r174	UTSW	7	23754343	missense	probably benign	0.01
R4589:Vmn1r174	UTSW	7	23754779	nonsense	probably null
R5175:Vmn1r174	UTSW	7	23754728	missense	probably benign	0.03
R5392:Vmn1r174	UTSW	7	23754802	missense	unknown
R5503:Vmn1r174	UTSW	7	23754137	missense	probably benign	0.03
R5568:Vmn1r174	UTSW	7	23754494	missense	probably damaging	0.96
R6705:Vmn1r174	UTSW	7	23754426	missense	probably benign	0.25
R8190:Vmn1r174	UTSW	7	23754143	missense	probably damaging	1.00
R8523:Vmn1r174	UTSW	7	23754757	missense	probably damaging	1.00
R8555:Vmn1r174	UTSW	7	23754545	missense	possibly damaging	0.84
R8913:Vmn1r174	UTSW	7	23753950	missense	possibly damaging	0.90
R8979:Vmn1r174	UTSW	7	23754467	missense	possibly damaging	0.92
R8990:Vmn1r174	UTSW	7	23754531	missense	possibly damaging	0.71
X0011:Vmn1r174	UTSW	7	23754481	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- GTCCACGATGCCATATTCATCAG -3'
(R):5'- CAGATGTTCTACCCAGGTTTATTGAG -3'

Sequencing Primer
(F):5'- CGATGCCATATTCATCAGCATCATGG -3'
(R):5'- CCCAGGTTTATTGAGTATCAGTGAC -3'

Posted On

2020-07-13