Incidental Mutation 'R8168:Nim1k'
ID633919
Institutional Source Beutler Lab
Gene Symbol Nim1k
Ensembl Gene ENSMUSG00000095930
Gene NameNIM1 serine/threonine protein kinase
SynonymsE130304F04Rik, Nim1
MMRRC Submission
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R8168 (G1)
Quality Score225.009
Status Validated
Chromosome13
Chromosomal Location119710094-119755882 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to T at 119712752 bp
ZygosityHeterozygous
Amino Acid Change Valine to Aspartic acid at position 202 (V202D)
Ref Sequence ENSEMBL: ENSMUSP00000136377 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000178142] [ENSMUST00000179869]
Predicted Effect probably damaging
Transcript: ENSMUST00000178142
AA Change: V202D

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)
SMART Domains Protein: ENSMUSP00000136377
Gene: ENSMUSG00000095930
AA Change: V202D

DomainStartEndE-ValueType
S_TKc 74 325 8.66e-92 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000179869
SMART Domains Protein: ENSMUSP00000136944
Gene: ENSMUSG00000093930

DomainStartEndE-ValueType
Pfam:HMG_CoA_synt_N 13 186 4e-111 PFAM
Pfam:HMG_CoA_synt_C 187 469 4e-134 PFAM
Meta Mutation Damage Score 0.6329 question?
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.6%
  • 20x: 98.7%
Validation Efficiency 100% (44/44)
Allele List at MGI
Other mutations in this stock
Total: 38 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1810043G02Rik G A 10: 77,982,944 A150T probably benign Het
9430020K01Rik A T 18: 4,675,094 D952V probably benign Het
AI607873 A C 1: 173,729,938 S411R probably benign Het
Ano4 A G 10: 88,980,995 I652T probably damaging Het
Arhgef1 A G 7: 24,925,406 T862A probably benign Het
Atp6v1b2 T C 8: 69,108,331 S404P possibly damaging Het
Cfap54 A T 10: 92,908,877 S2170T unknown Het
Copa A T 1: 172,099,672 H204L probably damaging Het
Cwc22 A C 2: 77,927,271 V171G probably damaging Het
D230025D16Rik C T 8: 105,248,769 P330L probably benign Het
Dock5 A T 14: 67,770,197 probably null Het
Flrt1 G A 19: 7,096,637 L182F probably damaging Het
Foxf1 T C 8: 121,085,162 V255A probably damaging Het
Gnptab G T 10: 88,419,133 A194S probably benign Het
Gzf1 C T 2: 148,684,766 R386C probably damaging Het
H2-Aa T A 17: 34,287,721 T16S possibly damaging Het
Hdgfrp2 T C 17: 56,082,282 F52S probably damaging Het
Htt A G 5: 34,882,956 N2154S probably benign Het
Lama3 T A 18: 12,506,942 W65R probably null Het
Mef2c G T 13: 83,656,350 L356F probably damaging Het
Mrgprb2 A T 7: 48,552,019 S319R probably benign Het
Mtrr C T 13: 68,572,613 V288I probably benign Het
Myo18a T A 11: 77,821,142 I713N probably damaging Het
Nelfa A T 5: 33,921,907 N107K possibly damaging Het
Nlrc4 T C 17: 74,445,211 T726A probably benign Het
Olfr1154 T A 2: 87,903,199 H159L probably damaging Het
Olfr1208 T A 2: 88,896,776 M274L probably benign Het
Prdm10 G A 9: 31,346,967 A514T probably benign Het
Prg4 T A 1: 150,455,850 E357D unknown Het
Ptp4a3 T G 15: 73,756,846 Y152D probably damaging Het
Raph1 A G 1: 60,499,620 C389R unknown Het
Rcc1 T C 4: 132,335,785 E170G probably benign Het
Spag17 C T 3: 100,034,984 T775M possibly damaging Het
Srcap T A 7: 127,542,523 V1825D probably damaging Het
Tex40 A G 19: 6,922,652 S162P possibly damaging Het
Tshr T A 12: 91,511,965 H195Q probably benign Het
Vmn1r174 A T 7: 23,754,671 D254V probably damaging Het
Vps13a A C 19: 16,749,548 I244M probably benign Het
Other mutations in Nim1k
AlleleSourceChrCoordTypePredicted EffectPPH Score
R1334:Nim1k UTSW 13 119712488 missense probably benign 0.05
R1782:Nim1k UTSW 13 119712151 missense probably benign 0.00
R2216:Nim1k UTSW 13 119714215 missense probably damaging 0.99
R3710:Nim1k UTSW 13 119712099 missense probably benign
R4385:Nim1k UTSW 13 119712626 missense probably damaging 0.98
R4430:Nim1k UTSW 13 119712542 missense possibly damaging 0.63
R4484:Nim1k UTSW 13 119712174 nonsense probably null
R4812:Nim1k UTSW 13 119712384 missense probably benign
R5383:Nim1k UTSW 13 119727799 missense probably benign 0.25
R5436:Nim1k UTSW 13 119727529 intron probably benign
R5511:Nim1k UTSW 13 119727594 missense probably damaging 1.00
R6682:Nim1k UTSW 13 119712188 missense probably benign 0.09
R6922:Nim1k UTSW 13 119727727 missense probably damaging 0.99
R7053:Nim1k UTSW 13 119727609 missense probably damaging 1.00
R7455:Nim1k UTSW 13 119712459 missense probably damaging 1.00
R8333:Nim1k UTSW 13 119712486 missense probably damaging 1.00
R8401:Nim1k UTSW 13 119712677 missense probably damaging 1.00
R8411:Nim1k UTSW 13 119714271 missense possibly damaging 0.95
R8515:Nim1k UTSW 13 119712450 nonsense probably null
R8540:Nim1k UTSW 13 119714182 missense probably benign 0.34
Z1177:Nim1k UTSW 13 119727702 missense probably benign 0.02
Predicted Primers PCR Primer
(F):5'- GTGCCAGTCACCATGAAGTAC -3'
(R):5'- CACTGGGTTAAAGGTCAGGG -3'

Sequencing Primer
(F):5'- GTGCCAGTCACCATGAAGTACAAAAG -3'
(R):5'- TTAAAGGTCAGGGCAGAGTTTG -3'
Posted On2020-07-13