Incidental Mutation 'R8168:Ptp4a3'
ID 633920
Institutional Source Beutler Lab
Gene Symbol Ptp4a3
Ensembl Gene ENSMUSG00000059895
Gene Name protein tyrosine phosphatase 4a3
Synonyms Prl-3
MMRRC Submission 067594-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.121) question?
Stock # R8168 (G1)
Quality Score 225.009
Status Validated
Chromosome 15
Chromosomal Location 73594991-73629075 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to G at 73628695 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Tyrosine to Aspartic acid at position 152 (Y152D)
Ref Sequence ENSEMBL: ENSMUSP00000060956 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000053232] [ENSMUST00000151999] [ENSMUST00000163582] [ENSMUST00000165541] [ENSMUST00000167582]
AlphaFold Q9D658
Predicted Effect probably damaging
Transcript: ENSMUST00000053232
AA Change: Y152D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000060956
Gene: ENSMUSG00000059895
AA Change: Y152D

DomainStartEndE-ValueType
Pfam:DSPc 26 155 7.1e-10 PFAM
Pfam:Y_phosphatase 27 153 3.9e-11 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000151999
SMART Domains Protein: ENSMUSP00000118236
Gene: ENSMUSG00000072487

DomainStartEndE-ValueType
low complexity region 5 16 N/A INTRINSIC
SCOP:d1gw5a_ 294 635 1e-2 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000163582
AA Change: Y152D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000131281
Gene: ENSMUSG00000059895
AA Change: Y152D

DomainStartEndE-ValueType
Pfam:DSPc 26 155 7.1e-10 PFAM
Pfam:Y_phosphatase 27 153 3.9e-11 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000165541
AA Change: Y152D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000132097
Gene: ENSMUSG00000059895
AA Change: Y152D

DomainStartEndE-ValueType
Pfam:Y_phosphatase 13 152 5.1e-9 PFAM
Pfam:DSPc 34 154 4.8e-9 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000167582
AA Change: Y133D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000131036
Gene: ENSMUSG00000059895
AA Change: Y133D

DomainStartEndE-ValueType
Pfam:Y_phosphatase 5 134 7.8e-12 PFAM
Pfam:DSPc 5 136 1.8e-10 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.6%
  • 20x: 98.7%
Validation Efficiency 100% (44/44)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the protein-tyrosine phosphatase family. Protein tyrosine phosphatases are cell signaling molecules that play regulatory roles in a variety of cellular processes. Studies of this class of protein tyrosine phosphatase in mice demonstrates that they are prenylated in vivo, suggesting their association with cell plasma membrane. The encoded protein may enhance cell proliferation, and overexpression of this gene has been implicated in tumor metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit partial preweaning lethality and decreased incidence of AOM-DDS induced tumors. Mice homozygous for a different targeted allele exhibit decreased circulating glucose levels in an intraperitoneal glucose tolerance test. [provided by MGI curators]
Allele List at MGI

All alleles(11) : Targeted(2) Gene trapped(9)
Other mutations in this stock
Total: 38 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ano4 A G 10: 88,816,857 (GRCm39) I652T probably damaging Het
Arhgef1 A G 7: 24,624,831 (GRCm39) T862A probably benign Het
Atp6v1b2 T C 8: 69,560,983 (GRCm39) S404P possibly damaging Het
Catsperz A G 19: 6,900,020 (GRCm39) S162P possibly damaging Het
Cfap410 G A 10: 77,818,778 (GRCm39) A150T probably benign Het
Cfap54 A T 10: 92,744,739 (GRCm39) S2170T unknown Het
Copa A T 1: 171,927,239 (GRCm39) H204L probably damaging Het
Cwc22 A C 2: 77,757,615 (GRCm39) V171G probably damaging Het
Dock5 A T 14: 68,007,646 (GRCm39) probably null Het
Flrt1 G A 19: 7,074,002 (GRCm39) L182F probably damaging Het
Foxf1 T C 8: 121,811,901 (GRCm39) V255A probably damaging Het
Gnptab G T 10: 88,254,995 (GRCm39) A194S probably benign Het
Gzf1 C T 2: 148,526,686 (GRCm39) R386C probably damaging Het
H2-Aa T A 17: 34,506,695 (GRCm39) T16S possibly damaging Het
Hdgfl2 T C 17: 56,389,282 (GRCm39) F52S probably damaging Het
Htt A G 5: 35,040,300 (GRCm39) N2154S probably benign Het
Ifi207 A C 1: 173,557,504 (GRCm39) S411R probably benign Het
Jcad A T 18: 4,675,094 (GRCm39) D952V probably benign Het
Lama3 T A 18: 12,639,999 (GRCm39) W65R probably null Het
Mef2c G T 13: 83,804,469 (GRCm39) L356F probably damaging Het
Mrgprb2 A T 7: 48,201,767 (GRCm39) S319R probably benign Het
Mtrr C T 13: 68,720,732 (GRCm39) V288I probably benign Het
Myo18a T A 11: 77,711,968 (GRCm39) I713N probably damaging Het
Nelfa A T 5: 34,079,251 (GRCm39) N107K possibly damaging Het
Nim1k A T 13: 120,174,288 (GRCm39) V202D probably damaging Het
Nlrc4 T C 17: 74,752,206 (GRCm39) T726A probably benign Het
Or4p8 T A 2: 88,727,120 (GRCm39) M274L probably benign Het
Or9m1 T A 2: 87,733,543 (GRCm39) H159L probably damaging Het
Phaf1 C T 8: 105,975,401 (GRCm39) P330L probably benign Het
Prdm10 G A 9: 31,258,263 (GRCm39) A514T probably benign Het
Prg4 T A 1: 150,331,601 (GRCm39) E357D unknown Het
Raph1 A G 1: 60,538,779 (GRCm39) C389R unknown Het
Rcc1 T C 4: 132,063,096 (GRCm39) E170G probably benign Het
Spag17 C T 3: 99,942,300 (GRCm39) T775M possibly damaging Het
Srcap T A 7: 127,141,695 (GRCm39) V1825D probably damaging Het
Tshr T A 12: 91,478,739 (GRCm39) H195Q probably benign Het
Vmn1r174 A T 7: 23,454,096 (GRCm39) D254V probably damaging Het
Vps13a A C 19: 16,726,912 (GRCm39) I244M probably benign Het
Other mutations in Ptp4a3
AlleleSourceChrCoordTypePredicted EffectPPH Score
P0005:Ptp4a3 UTSW 15 73,627,160 (GRCm39) missense possibly damaging 0.95
R2033:Ptp4a3 UTSW 15 73,625,618 (GRCm39) missense probably damaging 1.00
R2159:Ptp4a3 UTSW 15 73,623,865 (GRCm39) missense probably benign 0.00
R5976:Ptp4a3 UTSW 15 73,627,885 (GRCm39) missense possibly damaging 0.89
R6874:Ptp4a3 UTSW 15 73,595,259 (GRCm39) unclassified probably benign
R7894:Ptp4a3 UTSW 15 73,628,756 (GRCm39) missense probably benign 0.02
Predicted Primers PCR Primer
(F):5'- GCCTTCATGAAATCCAGGCTTC -3'
(R):5'- TTCAGAAAGACAGCCGTGG -3'

Sequencing Primer
(F):5'- TCTCCCAGAGTACCAGCTTAG -3'
(R):5'- CAGCCGTGGGTACAGATG -3'
Posted On 2020-07-13