Mutagenetix > Incidental Mutation

Incidental Mutation 'R8169:Olfr577'

633965

Institutional Source

Beutler Lab

Gene Symbol

Olfr577

Ensembl Gene

ENSMUSG00000043354

Gene Name

olfactory receptor 577

Synonyms

MOR7-2, GA_x6K02T2PBJ9-5685322-5684384

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.133) question?

Stock #

R8169 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

102971180-102975943 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 102973338 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 218 (L218P)

Ref Sequence

ENSEMBL: ENSMUSP00000059586 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000051505] [ENSMUST00000185326]

AlphaFold

Q8VH11

Predicted Effect

probably damaging
Transcript: ENSMUST00000051505
AA Change: L218P

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000059586
Gene: ENSMUSG00000043354
AA Change: L218P

Domain	Start	End	E-Value	Type
Pfam:7tm_4	34	312	3.3e-140	PFAM
Pfam:7TM_GPCR_Srsx	38	310	1.2e-6	PFAM
Pfam:7tm_1	44	295	7.5e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000185326

SMART Domains

Protein: ENSMUSP00000142459
Gene: ENSMUSG00000073962

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	35	300	9.7e-12	PFAM
Pfam:7tm_1	41	291	1.8e-29	PFAM
Pfam:7tm_4	140	284	2.6e-28	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

99% (80/81)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 83 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3110082I17Rik	C	A	5: 139,364,057	G79V	probably damaging	Het
4930562C15Rik	T	A	16: 4,866,218	Y226N	probably benign	Het
6430573F11Rik	A	T	8: 36,511,703	K153N	probably damaging	Het
Bach1	G	A	16: 87,722,502	C560Y	possibly damaging	Het
BC049730	T	A	7: 24,712,575	M60K	probably benign	Het
Capn13	T	A	17: 73,326,472		probably null	Het
Casq2	G	T	3: 102,110,312	A103S	possibly damaging	Het
Cfap100	A	G	6: 90,417,674	F57S		Het
Clca2	A	T	3: 145,077,892	L654Q	probably damaging	Het
Cntnap5c	T	A	17: 58,104,770		probably null	Het
Crtc2	G	C	3: 90,263,576	G652A	probably damaging	Het
Csnk1g2	A	G	10: 80,639,802	D401G	probably damaging	Het
Dars	T	C	1: 128,376,265	N242D	probably null	Het
Dnah10	T	C	5: 124,800,882	L2677P	probably damaging	Het
Dnal1	G	A	12: 84,124,556	A3T	probably benign	Het
Dpep2	C	T	8: 105,996,217	V60I		Het
Ehmt2	T	A	17: 34,903,363	I302N	probably benign	Het
Eif3a	C	T	19: 60,762,190	R1309Q	unknown	Het
Eno4	T	A	19: 58,946,652	Y100N	probably benign	Het
Ephx2	A	T	14: 66,112,153		probably null	Het
Fam102a	T	C	2: 32,563,748	I203T	probably benign	Het
Fbxw14	A	T	9: 109,277,216	I251K	probably benign	Het
Fcgbp	T	C	7: 28,085,494		probably null	Het
Foxm1	A	C	6: 128,371,708		probably null	Het
Fscn3	T	A	6: 28,430,329	I166N	possibly damaging	Het
Gdpd4	G	T	7: 97,972,128	V193L	probably benign	Het
Gm9573	T	C	17: 35,621,180	T705A	unknown	Het
Gnpat	T	G	8: 124,880,130	C352G	probably benign	Het
H2-Q7	T	A	17: 35,439,934	Y120*	probably null	Het
Hmha1	G	A	10: 80,027,872	A819T	probably damaging	Het
Klri1	G	A	6: 129,717,107	R6C	probably benign	Het
Kmo	T	C	1: 175,649,163	V154A	probably benign	Het
Kmt2c	A	G	5: 25,354,687	L1031P	probably damaging	Het
Lrrc14b	T	G	13: 74,363,167	T265P	possibly damaging	Het
Lrrc2	C	T	9: 110,980,886	T330I	probably benign	Het
Lyn	T	A	4: 3,783,050	S428T	probably damaging	Het
Mfsd8	T	A	3: 40,837,115	M59L	probably benign	Het
Mok	T	A	12: 110,808,365	Q341L	probably benign	Het
Myh3	C	A	11: 67,089,030	N598K	probably benign	Het
Nbeal1	G	C	1: 60,237,151	V684L	probably benign	Het
Nlrp4e	A	G	7: 23,320,506	I139M	probably benign	Het
Olfr139	T	A	11: 74,044,881	Q131L	possibly damaging	Het
Olfr142	G	A	2: 90,252,098	R297*	probably null	Het
Olfr667	A	T	7: 104,916,412	Y295N	possibly damaging	Het
Olfr741	T	C	14: 50,486,235	V259A	probably benign	Het
Osbpl7	G	A	11: 97,054,850	S312N	probably damaging	Het
Paxip1	A	G	5: 27,772,095	L323P	unknown	Het
Pcdh18	T	A	3: 49,745,235	H926L	probably damaging	Het
Pcm1	T	G	8: 41,310,116	S1460R	possibly damaging	Het
Pdha2	A	G	3: 141,211,394	S118P	possibly damaging	Het
Pdss1	A	T	2: 22,901,812	Y86F	probably benign	Het
Ppargc1a	A	T	5: 51,473,684	D534E	probably benign	Het
Prune2	A	G	19: 17,125,091	K2538R	probably benign	Het
Ptprq	A	T	10: 107,582,490	I1675N	probably damaging	Het
Rgma	C	T	7: 73,375,882	P3L	probably benign	Het
Ros1	A	G	10: 52,064,672		probably null	Het
Rsg1	A	G	4: 141,218,219	H127R	probably damaging	Het
Sema4g	C	T	19: 44,998,971	R519W	probably damaging	Het
Serpinc1	T	A	1: 160,993,401	F139L	probably damaging	Het
Slc22a29	A	G	19: 8,207,332	I198T	probably damaging	Het
Slc4a5	T	C	6: 83,303,391	V1007A	probably benign	Het
Sptbn1	T	C	11: 30,197,783	Y17C	possibly damaging	Het
Tex261	C	A	6: 83,775,017		probably null	Het
Timmdc1	T	A	16: 38,510,786	T128S	probably benign	Het
Tmem120b	C	A	5: 123,099,936	Y96*	probably null	Het
Tmem196	T	A	12: 120,018,576	F182I	possibly damaging	Het
Tnxb	G	A	17: 34,699,207	V2365M	possibly damaging	Het
Tph1	T	C	7: 46,653,809		silent	Het
Trpc1	G	A	9: 95,710,270	Q551*	probably null	Het
Tubgcp3	T	C	8: 12,616,099	N828D	probably benign	Het
Unc13a	C	A	8: 71,656,289	G478W	probably damaging	Het
Usp15	T	A	10: 123,125,893	T627S		Het
Vmn1r115	A	T	7: 20,844,219	I256N	probably damaging	Het
Vmn2r6	T	A	3: 64,539,889	K585N	probably benign	Het
Vsir	G	T	10: 60,358,268		probably null	Het
Xirp2	A	G	2: 67,513,199	D1928G	probably benign	Het
Xylt1	A	G	7: 117,650,619	Y672C	probably damaging	Het
Yrdc	A	G	4: 124,851,087	S105G	probably benign	Het
Zfc3h1	T	A	10: 115,418,711	N1403K	probably damaging	Het
Zfp160	A	T	17: 21,027,036	H616L	probably damaging	Het
Zfp637	T	A	6: 117,845,291	F127I	probably damaging	Het
Zfp69	G	T	4: 120,930,534	A528D	probably damaging	Het
Zpr1	T	A	9: 46,278,347	L342Q	possibly damaging	Het

Other mutations in Olfr577

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02246:Olfr577	APN	7	102973744	missense	possibly damaging	0.62
IGL03111:Olfr577	APN	7	102973531	missense	probably damaging	1.00
R1529:Olfr577	UTSW	7	102973879	missense	probably damaging	1.00
R1753:Olfr577	UTSW	7	102973056	missense	probably benign
R3005:Olfr577	UTSW	7	102973258	missense	possibly damaging	0.56
R4457:Olfr577	UTSW	7	102973527	missense	probably damaging	1.00
R4675:Olfr577	UTSW	7	102973806	missense	probably damaging	0.99
R4808:Olfr577	UTSW	7	102973911	missense	probably damaging	0.99
R4891:Olfr577	UTSW	7	102973552	missense	probably benign	0.12
R4917:Olfr577	UTSW	7	102973407	missense	possibly damaging	0.93
R4918:Olfr577	UTSW	7	102973407	missense	possibly damaging	0.93
R5328:Olfr577	UTSW	7	102973968	missense	possibly damaging	0.46
R6375:Olfr577	UTSW	7	102973753	missense	probably damaging	1.00
R6683:Olfr577	UTSW	7	102973713	missense	probably benign	0.05
R6958:Olfr577	UTSW	7	102973884	missense	possibly damaging	0.67
R7022:Olfr577	UTSW	7	102973968	missense	possibly damaging	0.46
R7429:Olfr577	UTSW	7	102973762	missense	probably damaging	1.00
R7430:Olfr577	UTSW	7	102973762	missense	probably damaging	1.00
R7490:Olfr577	UTSW	7	102973810	missense	probably damaging	1.00
R7808:Olfr577	UTSW	7	102973110	missense	possibly damaging	0.56
R8544:Olfr577	UTSW	7	102973731	missense	probably damaging	1.00
R9027:Olfr577	UTSW	7	102973353	missense	probably damaging	1.00
R9265:Olfr577	UTSW	7	102973905	nonsense	probably null
X0027:Olfr577	UTSW	7	102973686	missense	probably benign	0.05
Z1176:Olfr577	UTSW	7	102973309	missense	not run
Z1177:Olfr577	UTSW	7	102973309	missense	not run

Predicted Primers

PCR Primer
(F):5'- CATGACCACCTGAACTAGATGGG -3'
(R):5'- AATAGGTCTGGCCTCTCTGG -3'

Sequencing Primer
(F):5'- GGGGCCTGTTTCCCAAAG -3'
(R):5'- GGCCGTAGTGTTGCACTC -3'

Posted On

2020-07-13