Mutagenetix > Incidental Mutation

Incidental Mutation 'R0693:Or7g32'

63400

Institutional Source

Beutler Lab

Gene Symbol

Or7g32

Ensembl Gene

ENSMUSG00000052625

Gene Name

olfactory receptor family 7 subfamily G member 32

Synonyms

MOR155-1, GA_x6K02T2PVTD-13234278-13235216, Olfr851

MMRRC Submission

038878-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.127) question?

Stock #

R0693 (G1)

Quality Score

104

Status

Not validated

Chromosome

Chromosomal Location

19404591-19414111 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to A at 19389268 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Stop codon at position 90 (Q90*)

Ref Sequence

ENSEMBL: ENSMUSP00000148623 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077347] [ENSMUST00000211832]

AlphaFold

Q7TRG0

Predicted Effect

probably null
Transcript: ENSMUST00000077347
AA Change: Q93*

SMART Domains

Protein: ENSMUSP00000076569
Gene: ENSMUSG00000094535
AA Change: Q93*

Domain	Start	End	E-Value	Type
low complexity region	10	21	N/A	INTRINSIC
Pfam:7tm_4	34	311	1.8e-51	PFAM
Pfam:7TM_GPCR_Srsx	38	304	1e-6	PFAM
Pfam:7tm_1	44	293	5.1e-24	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000211832
AA Change: Q90*

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 94.8%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 20 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arhgef12	A	T	9: 42,929,697 (GRCm39)	N199K	probably damaging	Het
Ccnj	T	A	19: 40,825,551 (GRCm39)	L87H	probably damaging	Het
Cntn3	T	C	6: 102,145,908 (GRCm39)	T978A	possibly damaging	Het
Garnl3	A	G	2: 32,975,919 (GRCm39)	F16L	probably damaging	Het
Gbgt1	G	A	2: 28,394,842 (GRCm39)	G160D	probably damaging	Het
Gm5114	T	C	7: 39,058,188 (GRCm39)	D477G	probably benign	Het
Gp1ba	T	C	11: 70,531,284 (GRCm39)		probably benign	Het
Inpp4b	A	T	8: 82,723,943 (GRCm39)	T492S	probably benign	Het
Islr2	T	C	9: 58,107,027 (GRCm39)	T78A	possibly damaging	Het
Klhl9	T	G	4: 88,638,527 (GRCm39)	K571N	probably benign	Het
Msl3l2	G	A	10: 55,991,947 (GRCm39)	R224Q	possibly damaging	Het
Nfe2l3	A	G	6: 51,410,034 (GRCm39)	T50A	possibly damaging	Het
Or8b40	A	G	9: 38,027,325 (GRCm39)	T78A	probably benign	Het
Prkg1	T	A	19: 30,572,378 (GRCm39)	Q426L	probably benign	Het
Rnf215	G	A	11: 4,090,401 (GRCm39)		probably null	Het
Sp100	T	A	1: 85,594,726 (GRCm39)		probably null	Het
Tbc1d21	G	A	9: 58,268,570 (GRCm39)	T263M	probably damaging	Het
Tenm2	G	A	11: 35,915,636 (GRCm39)	T1966M	probably damaging	Het
Tnni3k	T	C	3: 154,667,609 (GRCm39)	Y268C	probably damaging	Het
Usp34	A	G	11: 23,402,637 (GRCm39)	T2477A	probably damaging	Het

Other mutations in Or7g32

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00094:Or7g32	APN	9	19,408,155 (GRCm39)	missense	probably damaging	1.00
IGL01992:Or7g32	APN	9	19,408,070 (GRCm39)	missense	probably benign	0.00
IGL02455:Or7g32	APN	9	19,408,559 (GRCm39)	nonsense	probably null
IGL02468:Or7g32	APN	9	19,408,473 (GRCm39)	missense	probably benign
IGL02685:Or7g32	APN	9	19,408,098 (GRCm39)	missense	probably benign
IGL02723:Or7g32	APN	9	19,388,805 (GRCm39)	missense	probably damaging	1.00
IGL03294:Or7g32	APN	9	19,389,285 (GRCm39)	missense	possibly damaging	0.95
PIT4305001:Or7g32	UTSW	9	19,389,357 (GRCm39)	missense	probably damaging	1.00
R0153:Or7g32	UTSW	9	19,408,233 (GRCm39)	missense	probably damaging	1.00
R0364:Or7g32	UTSW	9	19,389,268 (GRCm39)	nonsense	probably null
R0379:Or7g32	UTSW	9	19,388,776 (GRCm39)	missense	possibly damaging	0.75
R0449:Or7g32	UTSW	9	19,389,388 (GRCm39)	missense	possibly damaging	0.89
R0682:Or7g32	UTSW	9	19,388,645 (GRCm39)	missense	probably benign	0.03
R0789:Or7g32	UTSW	9	19,408,458 (GRCm39)	missense	possibly damaging	0.68
R1484:Or7g32	UTSW	9	19,389,423 (GRCm39)	missense	probably damaging	1.00
R1599:Or7g32	UTSW	9	19,389,517 (GRCm39)	missense	probably damaging	0.97
R1626:Or7g32	UTSW	9	19,389,495 (GRCm39)	missense	probably damaging	1.00
R1742:Or7g32	UTSW	9	19,389,337 (GRCm39)	missense	probably damaging	1.00
R2041:Or7g32	UTSW	9	19,408,131 (GRCm39)	missense	probably benign
R2060:Or7g32	UTSW	9	19,408,533 (GRCm39)	missense	possibly damaging	0.88
R4232:Or7g32	UTSW	9	19,389,022 (GRCm39)	missense	probably damaging	0.98
R4237:Or7g32	UTSW	9	19,388,893 (GRCm39)	missense	probably benign	0.00
R4474:Or7g32	UTSW	9	19,408,173 (GRCm39)	missense	probably damaging	1.00
R5081:Or7g32	UTSW	9	19,408,557 (GRCm39)	missense	probably benign	0.05
R5116:Or7g32	UTSW	9	19,389,094 (GRCm39)	missense	possibly damaging	0.67
R5643:Or7g32	UTSW	9	19,388,853 (GRCm39)	missense	probably benign	0.22
R6271:Or7g32	UTSW	9	19,389,337 (GRCm39)	missense	probably damaging	1.00
R6815:Or7g32	UTSW	9	19,389,061 (GRCm39)	missense	probably benign	0.20
R6853:Or7g32	UTSW	9	19,408,102 (GRCm39)	nonsense	probably null
R7150:Or7g32	UTSW	9	19,408,145 (GRCm39)	missense	probably benign	0.44
R7222:Or7g32	UTSW	9	19,388,763 (GRCm39)	missense	probably damaging	1.00
R7378:Or7g32	UTSW	9	19,408,398 (GRCm39)	missense	probably damaging	1.00
R7456:Or7g32	UTSW	9	19,408,844 (GRCm39)	missense	probably damaging	1.00
R7527:Or7g32	UTSW	9	19,408,685 (GRCm39)	missense	probably damaging	0.98
R7587:Or7g32	UTSW	9	19,408,818 (GRCm39)	missense	probably damaging	1.00
R7592:Or7g32	UTSW	9	19,389,128 (GRCm39)	missense	possibly damaging	0.52
R8155:Or7g32	UTSW	9	19,389,453 (GRCm39)	missense	probably benign	0.17
R8215:Or7g32	UTSW	9	19,408,796 (GRCm39)	missense	probably damaging	1.00
R8220:Or7g32	UTSW	9	19,408,317 (GRCm39)	missense	probably damaging	0.97
R8296:Or7g32	UTSW	9	19,408,377 (GRCm39)	missense	probably damaging	1.00
R8732:Or7g32	UTSW	9	19,408,098 (GRCm39)	missense	probably benign
R8813:Or7g32	UTSW	9	19,389,477 (GRCm39)	missense	possibly damaging	0.75
R9152:Or7g32	UTSW	9	19,408,448 (GRCm39)	missense	probably damaging	1.00
R9187:Or7g32	UTSW	9	19,389,166 (GRCm39)	missense	probably benign
R9528:Or7g32	UTSW	9	19,389,444 (GRCm39)	missense	probably damaging	1.00
R9789:Or7g32	UTSW	9	19,389,382 (GRCm39)	missense	probably benign	0.35
R9795:Or7g32	UTSW	9	19,408,412 (GRCm39)	missense	probably damaging	1.00
RF034:Or7g32	UTSW	9	19,388,928 (GRCm39)	missense	possibly damaging	0.46
X0058:Or7g32	UTSW	9	19,389,519 (GRCm39)	missense	probably benign	0.10
Z1177:Or7g32	UTSW	9	19,388,633 (GRCm39)	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- CAGGGTTCCATGATGACTGTGTACC -3'
(R):5'- GCTGGGAGGCAGTCTGTGATAATC -3'

Sequencing Primer
(F):5'- GAGACAGTTTTCCATGCCAC -3'
(R):5'- catcctgatctttatgctggtttc -3'

Posted On

2013-07-30