Mutagenetix > Incidental Mutation

Incidental Mutation 'R8179:Vmn2r86'

634562

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r86

Ensembl Gene

ENSMUSG00000092162

Gene Name

vomeronasal 2, receptor 86

Synonyms

EG625109

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.071) question?

Stock #

R8179 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

130445707-130455894 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 130453084 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Tyrosine at position 183 (H183Y)

Ref Sequence

ENSEMBL: ENSMUSP00000126596 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170257]

AlphaFold

G5E8Y4

Predicted Effect

probably benign
Transcript: ENSMUST00000170257
AA Change: H183Y

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000126596
Gene: ENSMUSG00000092162
AA Change: H183Y

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	425	1.1e-25	PFAM
Pfam:NCD3G	508	562	2.4e-19	PFAM
Pfam:7tm_3	595	829	6.4e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca6	A	G	11: 110,245,274	Y170H	probably damaging	Het
Ace3	G	T	11: 106,004,557	V602L	probably benign	Het
Ahr	T	C	12: 35,510,051	Q201R	probably benign	Het
Aldh18a1	A	C	19: 40,557,508	C612G	probably damaging	Het
Ankrd40	G	A	11: 94,334,715	A191T	probably benign	Het
Aox1	A	G	1: 58,097,958	D1169G	probably damaging	Het
Arfgap2	T	C	2: 91,275,323	F505L	probably damaging	Het
Arhgap40	T	C	2: 158,539,856	F423L	probably damaging	Het
Atf6b	T	C	17: 34,653,994	M628T	probably damaging	Het
Casp8ap2	T	A	4: 32,643,939	L1004*	probably null	Het
Cc2d2a	T	A	5: 43,699,953	L494Q	probably damaging	Het
Cd180	T	C	13: 102,705,633	S396P	probably benign	Het
Cdk19	T	C	10: 40,394,372	I59T	possibly damaging	Het
Cfap54	G	T	10: 92,997,316	F1149L	possibly damaging	Het
Chp1	C	T	2: 119,547,772		probably benign	Het
Clmp	T	C	9: 40,781,179	F248S	probably benign	Het
Ctc1	T	A	11: 69,024,224	I237K	probably benign	Het
Dcaf1	G	T	9: 106,857,916	V688L	probably damaging	Het
Ddx54	T	A	5: 120,627,102	M812K	probably benign	Het
Disc1	C	T	8: 125,087,577	P60L	probably benign	Het
Disp2	C	G	2: 118,792,549	P1254R	probably damaging	Het
Dnah11	T	A	12: 117,878,549	I4432F	possibly damaging	Het
Dnajc15	A	T	14: 77,852,953	I58N		Het
E330021D16Rik	A	G	6: 136,401,242	S197P	probably damaging	Het
Elovl5	A	G	9: 77,976,899	N159S	probably damaging	Het
Fgfr3	T	C	5: 33,727,755	V71A	probably benign	Het
Frs2	A	T	10: 117,076,886	H167Q	probably damaging	Het
Gm3286	T	C	5: 95,519,894	V111A	probably benign	Het
Gm4553	A	T	7: 142,164,857	V278E	unknown	Het
Grin2d	A	T	7: 45,858,028	Y416*	probably null	Het
Hmcn1	A	T	1: 150,722,514	V1679E	probably benign	Het
Igkv18-36	T	C	6: 69,992,495	Y105C	probably damaging	Het
Lingo1	T	C	9: 56,619,850	D491G	probably damaging	Het
Map3k1	A	C	13: 111,749,047	I1445M	probably damaging	Het
Med30	A	T	15: 52,712,568	Q20L	probably damaging	Het
Mgat5b	A	G	11: 116,931,728	E96G	probably benign	Het
Mmp16	T	A	4: 17,853,854		probably null	Het
Mogat1	A	G	1: 78,527,618	D176G	possibly damaging	Het
Mre11a	T	A	9: 14,797,066	D142E	probably null	Het
Mybpc2	C	A	7: 44,509,830	V599L	probably benign	Het
Myot	A	T	18: 44,354,130	R345*	probably null	Het
Olfr419	T	G	1: 174,250,564	D121A	possibly damaging	Het
Olfr968	C	T	9: 39,771,904	V299I	probably benign	Het
Olfr975	G	A	9: 39,950,412	R120C	probably benign	Het
Pglyrp2	T	C	17: 32,416,029	Y453C	possibly damaging	Het
Phrf1	A	G	7: 141,256,580	Y255C	unknown	Het
Plppr4	A	G	3: 117,331,678	S171P	probably damaging	Het
Ppip5k1	A	T	2: 121,341,614		probably null	Het
Rapgef3	A	T	15: 97,760,740	H170Q	probably benign	Het
Rnf10	ACCTCATCTCGTC	AC	5: 115,260,117		probably null	Het
Rpe65	A	G	3: 159,624,699	Y501C	probably benign	Het
Ruvbl2	A	G	7: 45,422,772	I346T	probably damaging	Het
Sema3g	A	G	14: 31,220,585	I48V	probably benign	Het
Slc4a10	A	C	2: 62,243,448	S285R	possibly damaging	Het
Slc6a11	G	A	6: 114,245,606	G521S	probably benign	Het
Slit3	A	G	11: 35,664,076	N912D	probably benign	Het
Traf3ip1	T	C	1: 91,500,801	V128A	unknown	Het
Trpc7	T	A	13: 56,887,880	N80I	probably damaging	Het
Ttc21b	G	A	2: 66,201,480	H1031Y	probably benign	Het
Tubg2	A	T	11: 101,160,256	I235F	probably benign	Het
Vmn2r118	A	T	17: 55,608,484	Y489N	probably benign	Het
Vmn2r54	G	T	7: 12,632,091	C305*	probably null	Het
Xrcc5	T	C	1: 72,356,857	V603A	probably damaging	Het
Zfp110	T	A	7: 12,844,571	Y136*	probably null	Het
Zfp112	C	T	7: 24,125,638	P348S	probably benign	Het

Other mutations in Vmn2r86

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01295:Vmn2r86	APN	10	130453026	missense	probably damaging	0.99
IGL01328:Vmn2r86	APN	10	130452496	missense	possibly damaging	0.78
IGL01377:Vmn2r86	APN	10	130452986	missense	probably damaging	0.99
IGL01548:Vmn2r86	APN	10	130446282	missense	probably benign	0.22
IGL01804:Vmn2r86	APN	10	130452989	missense	probably damaging	0.99
IGL01921:Vmn2r86	APN	10	130455741	missense	probably benign	0.00
IGL02406:Vmn2r86	APN	10	130448639	missense	possibly damaging	0.81
IGL02625:Vmn2r86	APN	10	130452912	missense	probably damaging	1.00
IGL02960:Vmn2r86	APN	10	130453767	missense	possibly damaging	0.74
IGL03104:Vmn2r86	APN	10	130446632	missense	probably damaging	1.00
R0408:Vmn2r86	UTSW	10	130446854	missense	probably damaging	1.00
R0437:Vmn2r86	UTSW	10	130446543	missense	probably damaging	1.00
R0577:Vmn2r86	UTSW	10	130452575	missense	probably benign	0.04
R0726:Vmn2r86	UTSW	10	130446396	missense	probably damaging	1.00
R0811:Vmn2r86	UTSW	10	130453628	missense	probably benign	0.00
R0812:Vmn2r86	UTSW	10	130453628	missense	probably benign	0.00
R1055:Vmn2r86	UTSW	10	130446357	missense	probably damaging	1.00
R1066:Vmn2r86	UTSW	10	130446276	missense	probably benign	0.01
R1199:Vmn2r86	UTSW	10	130448574	splice site	probably benign
R1332:Vmn2r86	UTSW	10	130446870	missense	probably damaging	1.00
R1568:Vmn2r86	UTSW	10	130453141	missense	probably benign	0.09
R1866:Vmn2r86	UTSW	10	130446386	missense	probably damaging	1.00
R1897:Vmn2r86	UTSW	10	130452445	missense	probably damaging	1.00
R2017:Vmn2r86	UTSW	10	130446713	missense	probably benign	0.39
R3162:Vmn2r86	UTSW	10	130455804	missense	probably damaging	0.99
R3162:Vmn2r86	UTSW	10	130455804	missense	probably damaging	0.99
R3858:Vmn2r86	UTSW	10	130455725	missense	probably benign
R4049:Vmn2r86	UTSW	10	130447097	missense	probably damaging	0.98
R4378:Vmn2r86	UTSW	10	130452600	missense	possibly damaging	0.67
R4411:Vmn2r86	UTSW	10	130452600	missense	possibly damaging	0.67
R4413:Vmn2r86	UTSW	10	130452600	missense	possibly damaging	0.67
R4422:Vmn2r86	UTSW	10	130452976	missense	possibly damaging	0.87
R4738:Vmn2r86	UTSW	10	130447070	missense	probably damaging	0.99
R4767:Vmn2r86	UTSW	10	130455737	missense	probably benign	0.00
R4872:Vmn2r86	UTSW	10	130453591	missense	probably damaging	0.98
R4880:Vmn2r86	UTSW	10	130453615	missense	probably benign	0.33
R5092:Vmn2r86	UTSW	10	130446587	missense	probably damaging	1.00
R5421:Vmn2r86	UTSW	10	130446936	missense	probably benign	0.41
R6007:Vmn2r86	UTSW	10	130453666	missense	probably damaging	1.00
R6330:Vmn2r86	UTSW	10	130446527	missense	probably benign	0.05
R6355:Vmn2r86	UTSW	10	130455894	start codon destroyed	probably damaging	0.98
R6397:Vmn2r86	UTSW	10	130446262	nonsense	probably null
R6419:Vmn2r86	UTSW	10	130446926	missense	probably damaging	1.00
R6933:Vmn2r86	UTSW	10	130446257	missense	probably damaging	1.00
R6937:Vmn2r86	UTSW	10	130448654	missense	probably damaging	1.00
R6959:Vmn2r86	UTSW	10	130446531	missense	probably damaging	1.00
R7010:Vmn2r86	UTSW	10	130455857	missense	probably benign
R7549:Vmn2r86	UTSW	10	130446828	missense	probably damaging	1.00
R8257:Vmn2r86	UTSW	10	130452410	missense	possibly damaging	0.87
R8286:Vmn2r86	UTSW	10	130449986	missense	probably benign	0.03
R8479:Vmn2r86	UTSW	10	130446866	missense	probably damaging	1.00
R8805:Vmn2r86	UTSW	10	130446527	missense	probably benign	0.05
R8960:Vmn2r86	UTSW	10	130453803	missense	probably benign	0.27
R9021:Vmn2r86	UTSW	10	130447065	missense	probably damaging	1.00
R9120:Vmn2r86	UTSW	10	130453808	missense	probably benign	0.00
R9137:Vmn2r86	UTSW	10	130446540	missense	probably damaging	1.00
R9311:Vmn2r86	UTSW	10	130452571	missense	probably damaging	1.00
R9312:Vmn2r86	UTSW	10	130452537	missense	probably benign	0.02
R9433:Vmn2r86	UTSW	10	130446698	missense	possibly damaging	0.88
R9696:Vmn2r86	UTSW	10	130449833	missense	possibly damaging	0.49

Predicted Primers

PCR Primer
(F):5'- TTTCTGGGATCACATTCACAAAAGC -3'
(R):5'- GGAAATTCATACTTGTCAGGAATAGCC -3'

Sequencing Primer
(F):5'- GCTAAACAAATACCATGCCTTTGG -3'
(R):5'- GGAGTTCTTAAAGGTGGGTA -3'

Posted On

2020-07-13